Restriction map for pF1KB1488
##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AgeI        
AscI        AvrII       BciVI       BfrI        BlnI        
BsaBI       BseAI       BsiWI       BsmBI       BspDI       
BspEI       BspLU11I    BssHII      Bst1107I    BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
EcoO65I     EcoRV       EcoT22I     FseI        HincII      
HindII      HindIII     HpaI        KspI        MamI        
MfeI        MluI        MroI        MunI        NheI        
NotI        NruI        NsiI        PacI        PinAI       
PmeI        Psp1406I    PvuI        RsrII       SacII       
SalI        ScaI        SexAI       SgrAI       SnaBI       
SpeI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII        4459  GACGTC               GACGT^C
Acc65I        470  GGTACC               G^GTACC
AcsI         2758  GAATTC               [GA]^AATT[CT]
AhdI          355  GACTCTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       4396  GTGCAC               G^TGCAC
AocI          316  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      2099  AGCGCT               AGC^GCT
ApaLI        4396  GTGCAC               G^TGCAC
ApoI         2758  GAATTC               [GA]^AATT[CT]
AseI         2059  ATTAAT               AT^TAAT
AsnI         2059  ATTAAT               AT^TAAT
Asp718I       470  GGTACC               G^GTACC
AspEI         355  GACTCTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         4579  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        3164  GGATCC               G^GATCC
BbvCI        4771  GCTGAGG              CCTCAGC(-5/-2)
BclI         5051  TGATCA               T^GATCA
BglII         641  AGATCT               A^GATCT
BlpI         1716  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1716  GCTCAGC              GC^T[ACGT]AGC
BsaWI        1742  ACCGGA               [AT]^CCGG[AT]
BsiEI        1429  CGGCCG               CG[GA][CT]^CG
Bsp1407I      572  TGTACA               T^GTACA
BspHI        1550  TCATGA               T^CATGA
BsrGI         572  TGTACA               T^GTACA
BstBI        2755  TTCGAA               TT^CGAA
Bsu36I        316  CCTGAGG              CC^T[ACGT]AGG
CelII        1716  GCTCAGC              GC^T[ACGT]AGC
DrdI         1997  GACTAAGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1429  CGGCCG               C^GGCCG
Eam1105I      355  GACTCTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1429  CGGCCG               C^GGCCG
Eco47III     2099  AGCGCT               AGC^GCT
Eco52I       1429  CGGCCG               C^GGCCG
Eco81I        316  CCTGAGG              CC^T[ACGT]AGG
EcoRI        2758  GAATTC               G^AATTC
FbaI         5051  TGATCA               T^GATCA
KpnI          470  GGTACC               GGTAC^C
NaeI         4650  GCCGGC               GCC^GGC
NdeI          839  CATATG               CA^TATG
NgoMIV       4650  GCCGGC               G^CCGGC
NspV         2755  TTCGAA               TT^CGAA
PflFI        4579  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        2059  ATTAAT               AT^TAAT
RcaI         1550  TCATGA               T^CATGA
SbfI         2280  CCTGCAGG             CCTGCA^GG
SfiI         2248  GGCCAGGCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         2755  TTCGAA               TT^CGAA
SphI         3380  GCATGC               GCATG^C
Sse8387I     2280  CCTGCAGG             CCTGCA^GG
SspBI         572  TGTACA               T^GTACA
Tth111I      4579  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AflIII       2122  ACACGT               A^C[GA][CT]GT
             2603  ACGTGT              
ApaI         1142  GGGCCC               GGGCC^C
             4555  GGGCCC              
Asp700I       876  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4008  GAATGGGTTC          
BbeI         3612  GGCGCC               GGCGC^C
             4362  GGCGCC              
BbrPI        2412  CACGTG               CAC^GTG
             2602  CACGTG              
BcgI         5008  CGAGCCTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4445  GCATGGCCATCG        
BfaI          859  CTAG                 C^TAG
             1786  CTAG                
BsmI         1199  GCATTC               GAATGC(1/-1)
             1295  GCATTC              
BsrFI        4632  GCCGGT               [GA]^CCGG[CT]
             4650  GCCGGC              
BssSI        3823  CACGAG               CACGAG(-5/-1)
             1448  CTCGTG              
Cfr10I       4632  GCCGGT               [GA]^CCGG[CT]
             4650  GCCGGC              
KasI         3612  GGCGCC               G^GCGCC
             4362  GGCGCC              
MaeI          859  CTAG                 C^TAG
             1786  CTAG                
NarI         3612  GGCGCC               GG^CGCC
             4362  GGCGCC              
PmaCI        2412  CACGTG               CAC^GTG
             2602  CACGTG              
PmlI         2412  CACGTG               CAC^GTG
             2602  CACGTG              
PshAI        1578  GACCTCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3355  GACCCCTGTC          
SacI         1066  GAGCTC               GAGCT^C
             1714  GAGCTC              
SfoI         3612  GGCGCC               GGC^GCC
             4362  GGCGCC              
SmaI         1408  CCCGGG               CCC^GGG
             4595  CCCGGG              
SspI         2379  AATATT               AAT^ATT
             5045  AATATT              
StuI         1160  AGGCCT               AGG^CCT
             2451  AGGCCT              
XmaCI        1408  CCCGGG               C^CCGGG
             4595  CCCGGG              
XmaI         1408  CCCGGG               C^CCGGG
             4595  CCCGGG              
XmnI          876  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4008  GAATGGGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaI        AvaII       
AviII       BalI        BanI        BanII       BbsI        
BbvI        BcnI        BglI        BmyI        BpmI        
BpuAI       BsaAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrDI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PaeR7I      PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoI        XhoII       
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