Restriction map for pF1KB1488
 (Linear) (Six-base) MAPSORT of: KIBB1488.seq  Check: 5892  from: 1  to: 5232



KIBB1488.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2510    2646    5232 
   Size:       2510     136    2586

  Fragments arranged by size: 

                2586    2510     136
 
AatII G_ACGT'C

Cuts at:      0    4463    5232 
   Size:       4463     769
 
Acc65I G'GTAC_C

Cuts at:      0     470    5232 
   Size:        470    4762
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1268    1382    1915    2752    4153    4775    5232 
   Size:       1268     114     533     837    1401     622     457

  Fragments arranged by size: 

                1401    1268     837     622     533     457     114
 
AfeI AGC'GCT

Cuts at:      0    2101    5232 
   Size:       2101    3131
 
AflIII A'CryG_T

Cuts at:      0    2122    2603    5232 
   Size:       2122     481    2629

  Fragments arranged by size: 

                2629    2122     481
 
AhdI GACnn_n'nnGTC

Cuts at:      0     360    5232 
   Size:        360    4872
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1738    1770    5232 
   Size:       1738      32    3462

  Fragments arranged by size: 

                3462    1738      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1005    1195    1345    2661    3227    3700    5103    5232 
   Size:       1005     190     150    1316     566     473    1403     129

  Fragments arranged by size: 

                1403    1316    1005     566     473     190     150     129
            
 
ApaI G_GGCC'C

Cuts at:      0    1146    4559    5232 
   Size:       1146    3413     673

  Fragments arranged by size: 

                3413    1146     673
 
ApaLI G'TGCA_C

Cuts at:      0    4396    5232 
   Size:       4396     836
 
ApoI r'AATT_y

Cuts at:      0    2758    5232 
   Size:       2758    2474
 
AseI AT'TA_AT

Cuts at:      0    2060    5232 
   Size:       2060    3172
 
AvaI C'yCGr_G

Cuts at:      0     140     504    1408    1532    2744    3020    3115    4595 
   Size:        140     364     904     124    1212     276      95    1480

Cuts at:   4595    4684    5083    5232 
   Size:         89     399     149

  Fragments arranged by size: 

                1480    1212     904     399     364     276     149     140
                 124      95      89
 
BamHI G'GATC_C

Cuts at:      0    3164    5232 
   Size:       3164    2068
 
BanI G'GyrC_C

Cuts at:      0     264     416     470     762    1611    1729    2664    2703 
   Size:        264     152      54     292     849     118     935      39

Cuts at:   2703    3565    3612    4362    4713    5222    5232 
   Size:        862      47     750     351     509      10

  Fragments arranged by size: 

                 935     862     849     750     509     351     292     264
                 152     118      54      47      39      10
 
BanII G_rGCy'C

Cuts at:      0     857    1070    1146    1565    1718    2192    2271    2677 
   Size:        857     213      76     419     153     474      79     406

Cuts at:   2677    3560    3694    4559    5232 
   Size:        883     134     865     673

  Fragments arranged by size: 

                 883     865     857     673     474     419     406     213
                 153     134      79      76
 
BbeI G_GCGC'C

Cuts at:      0    3616    4366    5232 
   Size:       3616     750     866

  Fragments arranged by size: 

                3616     866     750
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     883    1220    2023    3456    4907    5232 
   Size:        883     337     803    1433    1451     325

  Fragments arranged by size: 

                1451    1433     883     803     337     325
 
BbvCI CC'TCA_GC

Cuts at:      0    4772    5232 
   Size:       4772     460
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4434    4468    4997    5031    5232 
   Size:       4434      34     529      34     201

  Fragments arranged by size: 

                4434     529     201      34      34
 
BclI T'GATC_A

Cuts at:      0    5051    5232 
   Size:       5051     181
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1795    2255    3586    4638    5232 
   Size:       1795     460    1331    1052     594

  Fragments arranged by size: 

                1795    1331    1052     594     460
 
BglII A'GATC_T

Cuts at:      0     641    5232 
   Size:        641    4591
 
BlpI GC'TnA_GC

Cuts at:      0    1717    5232 
   Size:       1717    3515
 
Bme1580I G_kGCm'C

Cuts at:      0     140     269     419    1146    1929    2708    3570    4400 
   Size:        140     129     150     727     783     779     862     830

Cuts at:   4400    4559    4716    4786    5184    5232 
   Size:        159     157      70     398      48

  Fragments arranged by size: 

                 862     830     783     779     727     398     159     157
                 150     140     129      70      48
 
BmgBI CAC'GTC

Cuts at:      0    2125    4218    4563    5232 
   Size:       2125    2093     345     669

  Fragments arranged by size: 

                2125    2093     669     345
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     681    1651    2464    2605    3696    5091    5232 
   Size:        681     970     813     141    1091    1395     141

  Fragments arranged by size: 

                1395    1091     970     813     681     141     141
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3035    5154    5232 
   Size:       3035    2119      78

  Fragments arranged by size: 

                3035    2119      78
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     244     916     982     988    1025    1732    2293 
   Size:        112     132     672      66       6      37     707     561

Cuts at:   2293    2370    2566    3692    4081    4522    4825    5185    5232 
   Size:         77     196    1126     389     441     303     360      47

  Fragments arranged by size: 

                1126     707     672     561     441     389     360     303
                 196     132     112      77      66      47      37       6
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     226    2431    4772    5232 
   Size:        226    2205    2341     460

  Fragments arranged by size: 

                2341    2205     460     226
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3030    5232 
   Size:       3030    2202
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1590    1643    1964    3112    3982    4540    4924    5232 
   Size:       1590      53     321    1148     870     558     384     308

  Fragments arranged by size: 

                1590    1148     870     558     384     321     308      53
            
 
BsaAI yAC'GTr

Cuts at:      0    2414    2604    4533    5232 
   Size:       2414     190    1929     699

  Fragments arranged by size: 

                2414    1929     699     190
 
BsaHI Gr'CG_yC

Cuts at:      0     962    3613    4363    4460    5232 
   Size:        962    2651     750      97     772

  Fragments arranged by size: 

                2651     962     772     750      97
 
BsaWI w'CCGG_w

Cuts at:      0    1742    5232 
   Size:       1742    3490
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2378    2408    2608    2638    2762    2792    3096    3126 
   Size:       2378      30     200      30     124      30     304      30

Cuts at:   3126    3720    3750    4060    4090    4372    4402    5232 
   Size:        594      30     310      30     282      30     830

  Fragments arranged by size: 

                2378     830     594     310     304     282     200     124
                  30      30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     241     295     393    1072    1436    2302    2839    3728 
   Size:        241      54      98     679     364     866     537     889

Cuts at:   3728    3982    4078    4081    4393    5143    5232 
   Size:        254      96       3     312     750      89

  Fragments arranged by size: 

                 889     866     750     679     537     364     312     254
                 241      98      96      89      54       3
 
BseYI C'CCAG_C

Cuts at:      0    1188    1690    1800    2632    2651    3326    3553    3771 
   Size:       1188     502     110     832      19     675     227     218

Cuts at:   3771    5183    5232 
   Size:       1412      49

  Fragments arranged by size: 

                1412    1188     832     675     502     227     218     110
                  49      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     847    2188    2488    3500    3793    4642    4666 
   Size:        142     705    1341     300    1012     293     849      24

Cuts at:   4666    4936    5232 
   Size:        270     296

  Fragments arranged by size: 

                1341    1012     849     705     300     296     293     270
                 142      24
 
BsiEI CG_ry'CG

Cuts at:      0    1432    5232 
   Size:       1432    3800
 
BsiHKAI G_wGCw'C

Cuts at:      0     511    1070    1718    3106    4400    5161    5232 
   Size:        511     559     648    1388    1294     761      71

  Fragments arranged by size: 

                1388    1294     761     648     559     511      71
 
BsmI GAATG_Cn'

Cuts at:      0    1199    1295    5232 
   Size:       1199      96    3937

  Fragments arranged by size: 

                3937    1199      96
 
Bsp1286I G_dGCh'C

Cuts at:      0     140     269     419     511     857    1070    1146    1565 
   Size:        140     129     150      92     346     213      76     419

Cuts at:   1565    1718    1929    2192    2271    2677    2708    3106    3560 
   Size:        153     211     263      79     406      31     398     454

Cuts at:   3560    3570    3694    4400    4559    4716    4786    5161    5184 
   Size:         10     124     706     159     157      70     375      23

Cuts at:   5184    5232 
   Size:         48

  Fragments arranged by size: 

                 706     454     419     406     398     375     346     263
                 213     211     159     157     153     150     140     129
                 124      92      79      76      70      48      31      23
                  10
 
BspHI T'CATG_A

Cuts at:      0    1550    5232 
   Size:       1550    3682
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     401    2510    2646    3264    3632    4186    5111    5232 
   Size:        401    2109     136     618     368     554     925     121

  Fragments arranged by size: 

                2109     925     618     554     401     368     136     121
            
 
BsrBI CCG'CTC

Cuts at:      0    1283    1428    4809    5232 
   Size:       1283     145    3381     423

  Fragments arranged by size: 

                3381    1283     423     145
 
BsrDI GCAATG_nn'

Cuts at:      0    3408    3662    3720    5232 
   Size:       3408     254      58    1512

  Fragments arranged by size: 

                3408    1512     254      58
 
BsrFI r'CCGG_y

Cuts at:      0    4632    4650    5232 
   Size:       4632      18     582

  Fragments arranged by size: 

                4632     582      18
 
BsrGI T'GTAC_A

Cuts at:      0     572    5232 
   Size:        572    4660
 
BssSI C'ACGA_G

Cuts at:      0    1448    3823    5232 
   Size:       1448    2375    1409

  Fragments arranged by size: 

                2375    1448    1409
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3816    4629    5179    5232 
   Size:       3816     813     550      53

  Fragments arranged by size: 

                3816     813     550      53
 
BstBI TT'CG_AA

Cuts at:      0    2756    5232 
   Size:       2756    2476
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     635    2342    3670    3779    4374    5209    5232 
   Size:        635    1707    1328     109     595     835      23

  Fragments arranged by size: 

                1707    1328     835     635     595     109      23
 
BstYI r'GATC_y

Cuts at:      0     641    1107    2276    2420    2949    3138    3164    5232 
   Size:        641     466    1169     144     529     189      26    2068

  Fragments arranged by size: 

                2068    1169     641     529     466     189     144      26
            
 
Bsu36I CC'TnA_GG

Cuts at:      0     317    5232 
   Size:        317    4915
 
BtgI C'CryG_G

Cuts at:      0     343    1220    1484    1644    2424    2708    3134    5232 
   Size:        343     877     264     160     780     284     426    2098

  Fragments arranged by size: 

                2098     877     780     426     343     284     264     160
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3423    4437    5232 
   Size:       3423    1014     795

  Fragments arranged by size: 

                3423    1014     795
 
BtsI GCAGTG_nn'

Cuts at:      0    1497    3103    3106    5232 
   Size:       1497    1606       3    2126

  Fragments arranged by size: 

                2126    1606    1497       3
 
DraIII CAC_nnn'GTG

Cuts at:      0    1134    1659    3135    4409    4745    5232 
   Size:       1134     525    1476    1274     336     487

  Fragments arranged by size: 

                1476    1274    1134     525     487     336
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2003    5232 
   Size:       2003    3229
 
EaeI y'GGCC_r

Cuts at:      0     456    1284    1429    1661    2462    3458    4448    4652 
   Size:        456     828     145     232     801     996     990     204

Cuts at:   4652    4736    5232 
   Size:         84     496

  Fragments arranged by size: 

                 996     990     828     801     496     456     232     204
                 145      84
 
EagI C'GGCC_G

Cuts at:      0    1429    5232 
   Size:       1429    3803
 
EarI CTCTTCn'nnn_

Cuts at:      0     277     403    2227    5232 
   Size:        277     126    1824    3005

  Fragments arranged by size: 

                3005    1824     277     126
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2500    2518    2654    3199    4434    4909    5232 
   Size:       2500      18     136     545    1235     475     323

  Fragments arranged by size: 

                2500    1235     545     475     323     136      18
 
EcoICRI GAG'CTC

Cuts at:      0    1068    1716    5232 
   Size:       1068     648    3516

  Fragments arranged by size: 

                3516    1068     648
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     244     916     982     988    1025    1268    1382 
   Size:        112     132     672      66       6      37     243     114

Cuts at:   1382    1732    1915    2293    2370    2566    2752    3692    4081 
   Size:        350     183     378      77     196     186     940     389

Cuts at:   4081    4153    4522    4775    4825    5185    5232 
   Size:         72     369     253      50     360      47

  Fragments arranged by size: 

                 940     672     389     378     369     360     350     253
                 243     196     186     183     132     114     112      77
                  72      66      50      47      37       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     862     943    1156    1400    2284    4063    5232 
   Size:        862      81     213     244     884    1779    1169

  Fragments arranged by size: 

                1779    1169     884     862     244     213      81
 
EcoO109I rG'GnC_Cy

Cuts at:      0     102     313     632    1142    1143    1405    1571    1694 
   Size:        102     211     319     510       1     262     166     123

Cuts at:   1694    2292    2750    2978    3252    3354    3768    4137    4185 
   Size:        598     458     228     274     102     414     369      48

Cuts at:   4185    4555    4556    5232 
   Size:        370       1     676

  Fragments arranged by size: 

                 676     598     510     458     414     370     369     319
                 274     262     228     211     166     123     102     102
                  48       1       1
 
EcoRI G'AATT_C

Cuts at:      0    2758    5232 
   Size:       2758    2474
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     234     900    1785    5232 
   Size:        234     666     885    3447

  Fragments arranged by size: 

                3447     885     666     234
 
FspI TGC'GCA

Cuts at:      0    2227    2323    4993    5232 
   Size:       2227      96    2670     239

  Fragments arranged by size: 

                2670    2227     239      96
 
HaeII r_GCGC'y

Cuts at:      0    1801    2103    3616    4366    5232 
   Size:       1801     302    1513     750     866

  Fragments arranged by size: 

                1801    1513     866     750     302
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     346     378    1057    1089    1526    1558    1667    1699 
   Size:        346      32     679      32     437      32     109      32

Cuts at:   1699    2764    2796    3919    3951    4372    4404    5043    5075 
   Size:       1065      32    1123      32     421      32     639      32

Cuts at:   5075    5232 
   Size:        157

  Fragments arranged by size: 

                1123    1065     679     639     437     421     346     157
                 109      32      32      32      32      32      32      32
                  32
 
KasI G'GCGC_C

Cuts at:      0    3612    4362    5232 
   Size:       3612     750     870

  Fragments arranged by size: 

                3612     870     750
 
KpnI G_GTAC'C

Cuts at:      0     474    5232 
   Size:        474    4758
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     698    2286    3082    3776    4318    5232 
   Size:        698    1588     796     694     542     914

  Fragments arranged by size: 

                1588     914     796     698     694     542
 
MscI TGG'CCA

Cuts at:      0     458    1663    2464    4450    4738    5232 
   Size:        458    1205     801    1986     288     494

  Fragments arranged by size: 

                1986    1205     801     494     458     288
 
MslI CAynn'nnrTG

Cuts at:      0     526     776    2317    3655    5232 
   Size:        526     250    1541    1338    1577

  Fragments arranged by size: 

                1577    1541    1338     526     250
 
MspA1I CmG'CkG

Cuts at:      0     327     462    1948    2154    2544    2722    3577    4035 
   Size:        327     135    1486     206     390     178     855     458

Cuts at:   4035    4326    4978    5169    5232 
   Size:        291     652     191      63

  Fragments arranged by size: 

                1486     855     652     458     390     327     291     206
                 191     178     135      63
 
NaeI GCC'GGC

Cuts at:      0    4652    5232 
   Size:       4652     580
 
NarI GG'CG_CC

Cuts at:      0    3613    4363    5232 
   Size:       3613     750     869

  Fragments arranged by size: 

                3613     869     750
 
NcoI C'CATG_G

Cuts at:      0     343    1644    2424    5232 
   Size:        343    1301     780    2808

  Fragments arranged by size: 

                2808    1301     780     343
 
NdeI CA'TA_TG

Cuts at:      0     840    5232 
   Size:        840    4392
 
NgoMIV G'CCGG_C

Cuts at:      0    4650    5232 
   Size:       4650     582
 
NspI r_CATG'y

Cuts at:      0     579    3162    3384    5232 
   Size:        579    2583     222    1848

  Fragments arranged by size: 

                2583    1848     579     222
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     330    1195    1608    1659    3135    5232 
   Size:        330     865     413      51    1476    2097

  Fragments arranged by size: 

                2097    1476     865     413     330      51
 
PfoI T'CCnGG_A

Cuts at:      0     128     593     956    1253    3350    3467    4157    5201 
   Size:        128     465     363     297    2097     117     690    1044

Cuts at:   5201    5232 
   Size:         31

  Fragments arranged by size: 

                2097    1044     690     465     363     297     128     117
                  31
 
PmlI CAC'GTG

Cuts at:      0    2414    2604    5232 
   Size:       2414     190    2628

  Fragments arranged by size: 

                2628    2414     190
 
PpuMI rG'GwC_Cy

Cuts at:      0     102     632    1571    2292    2750    3252    3354    4137 
   Size:        102     530     939     721     458     502     102     783

Cuts at:   4137    4185    5232 
   Size:         48    1047

  Fragments arranged by size: 

                1047     939     783     721     530     502     458     102
                 102      48
 
PshAI GACnn'nnGTC

Cuts at:      0    1582    3359    5232 
   Size:       1582    1777    1873

  Fragments arranged by size: 

                1873    1777    1582
 
PspOMI G'GGCC_C

Cuts at:      0    1142    4555    5232 
   Size:       1142    3413     677

  Fragments arranged by size: 

                3413    1142     677
 
PspXI vC'TCGA_Gb

Cuts at:      0    2744    3020    5083    5232 
   Size:       2744     276    2063     149

  Fragments arranged by size: 

                2744    2063     276     149
 
PstI C_TGCA'G

Cuts at:      0      56    1026    1160    1334    1350    1833    2285    2795 
   Size:         56     970     134     174      16     483     452     510

Cuts at:   2795    3302    4034    4076    4307    4325    4820    5232 
   Size:        507     732      42     231      18     495     412

  Fragments arranged by size: 

                 970     732     510     507     495     483     452     412
                 231     174     134      56      42      18      16
 
PvuII CAG'CTG

Cuts at:      0     327    2544    3577    4035    4326    5232 
   Size:        327    2217    1033     458     291     906

  Fragments arranged by size: 

                2217    1033     906     458     327     291
 
SacI G_AGCT'C

Cuts at:      0    1070    1718    5232 
   Size:       1070     648    3514

  Fragments arranged by size: 

                3514    1070     648
 
SanDI GG'GwC_CC

Cuts at:      0     102    5232 
   Size:        102    5130
 
SapI GCTCTTCn'nnn_

Cuts at:      0     277     403    2227    5232 
   Size:        277     126    1824    3005

  Fragments arranged by size: 

                3005    1824     277     126
 
SbfI CC_TGCA'GG

Cuts at:      0    2285    5232 
   Size:       2285    2947
 
SfcI C'TryA_G

Cuts at:      0      52    1022    1156    1330    1346    1829    2281    2791 
   Size:         52     970     134     174      16     483     452     510

Cuts at:   2791    3298    4030    4072    4189    4303    4321    4816    4833 
   Size:        507     732      42     117     114      18     495      17

Cuts at:   4833    5232 
   Size:        399

  Fragments arranged by size: 

                 970     732     510     507     495     483     452     399
                 174     134     117     114      52      42      18      17
                  16
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2255    5232 
   Size:       2255    2977
 
SfoI GGC'GCC

Cuts at:      0    3614    4364    5232 
   Size:       3614     750     868

  Fragments arranged by size: 

                3614     868     750
 
SmaI CCC'GGG

Cuts at:      0    1410    4597    5232 
   Size:       1410    3187     635

  Fragments arranged by size: 

                3187    1410     635
 
SmlI C'TyrA_G

Cuts at:      0    2744    3009    3020    5083    5232 
   Size:       2744     265      11    2063     149

  Fragments arranged by size: 

                2744    2063     265     149      11
 
SphI G_CATG'C

Cuts at:      0    3384    5232 
   Size:       3384    1848
 
SspI AAT'ATT

Cuts at:      0    2381    5047    5232 
   Size:       2381    2666     185

  Fragments arranged by size: 

                2666    2381     185
 
StuI AGG'CCT

Cuts at:      0    1162    2453    5232 
   Size:       1162    1291    2779

  Fragments arranged by size: 

                2779    1291    1162
 
StyI C'CwwG_G

Cuts at:      0     343    1103    1163    1644    1808    2009    2424    2783 
   Size:        343     760      60     481     164     201     415     359

Cuts at:   2783    2849    2870    3122    3608    3885    4082    4280    4367 
   Size:         66      21     252     486     277     197     198      87

Cuts at:   4367    4961    5232 
   Size:        594     271

  Fragments arranged by size: 

                 760     594     486     481     415     359     343     277
                 271     252     201     198     197     164      87      66
                  60      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     700    1572    1670    3553    4564    4572    4957    5232 
   Size:        700     872      98    1883    1011       8     385     275

  Fragments arranged by size: 

                1883    1011     872     700     385     275      98       8
            
 
TatI w'GTAC_w

Cuts at:      0     572    3683    4223    5232 
   Size:        572    3111     540    1009

  Fragments arranged by size: 

                3111    1009     572     540
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     137     169    2378    2410    2608    2640    2762    2794 
   Size:        137      32    2209      32     198      32     122      32

Cuts at:   2794    3096    3128    3720    3752    5232 
   Size:        302      32     592      32    1480

  Fragments arranged by size: 

                2209    1480     592     302     198     137     122      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    4582    5232 
   Size:       4582     650
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     525    1380    3048    3615    5232 
   Size:        525     855    1668     567    1617

  Fragments arranged by size: 

                1668    1617     855     567     525
 
XhoI C'TCGA_G

Cuts at:      0    2744    3020    5083    5232 
   Size:       2744     276    2063     149

  Fragments arranged by size: 

                2744    2063     276     149
 
XmaI C'CCGG_G

Cuts at:      0    1408    4595    5232 
   Size:       1408    3187     637

  Fragments arranged by size: 

                3187    1408     637
 
XmnI GAAnn'nnTTC

Cuts at:      0     880    4012    5232 
   Size:        880    3132    1220

  Fragments arranged by size: 

                3132    1220     880
 
ZraI GAC'GTC

Cuts at:      0    4461    5232 
   Size:       4461     771
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI     AfeI   AflIII     AhdI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI    PflMI     PfoI
     PmlI    PpuMI    PshAI   PspOMI    PspXI     PstI    PvuII     SacI
    SanDI     SapI     SbfI     SfcI     SfiI     SfoI     SmaI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI     AclI    AflII     AgeI     AleI     AlfI     AscI    AsiSI
    AvrII     BaeI    BciVI    BfrBI     BmtI    BsaBI    BsiWI    BsmBI
    BspEI   BssHII   BstEII  BstZ17I     ClaI    CspCI     DraI    EcoRV
     FseI    FspAI   HincII  HindIII     HpaI     MfeI     MluI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PpiI
     PsiI     PsrI     PvuI    RsrII    SacII     SalI     ScaI    SexAI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SwaI     XbaI
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