Restriction map for pF1KB0065
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AflII       
AgeI        Alw44I      ApaLI       AscI        Asp718I     
AvaI        AviII       BbeI        BbrPI       BcgI        
BfrI        BsaBI       BseAI       BsiEI       BsiWI       
BsoBI       BspDI       BspEI       BspHI       BsrBI       
BsrFI       BssHII      Bst1107I    BstUI       BstZ17I     
Cfr10I      ClaI        CpoI        DrdI        EagI        
EclXI       Eco52I      EcoNI       EcoRI       EcoRV       
FseI        FspI        KasI        KpnI        KspI        
MamI        MluI        MroI        MvnI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SwaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI         3180  GTCTAC               GT^[AC][GT]AC
AflIII       3564  ACATGT               A^C[GA][CT]GT
AhdI         4565  GACTCAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI        44  AGCGCT               AGC^GCT
AspEI        4565  GACTCAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         5257  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         2072  TGGCCA               TGG^CCA
BbvCI         676  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1548  GTATCC               GTATCC(6/5)
BcnI         5194  CCGGG                CC^[GC]GG
BglI         3832  GCCGACAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1972  CACGTA               [CT]AC^GT[GA]
Bsp1407I      162  TGTACA               T^GTACA
BspLU11I     3564  ACATGT               A^CATGT
BsrGI         162  TGTACA               T^GTACA
BssSI        3209  CACGAG               CACGAG(-5/-1)
BstBI        3098  TTCGAA               TT^CGAA
DraI         4432  TTTAAA               TTT^AAA
DsaI         4553  CCACGG               C^C[GA][CT]GG
Eam1105I     4565  GACTCAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III       44  AGCGCT               AGC^GCT
HaeII          44  AGCGCT               [GA]GCGC^[CT]
HgaI         5097  GACGC                GACGC(5/10)
HindIII      3297  AAGCTT               A^AGCTT
MluNI        2072  TGGCCA               TGG^CCA
MscI         2072  TGGCCA               TGG^CCA
NciI         5194  CCGGG                CC^[GC]GG
NspV         3098  TTCGAA               TT^CGAA
PflFI        5257  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        5995  GACGGACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI         1776  GCTCTTC              GCTCTTC(1/4)
ScaI         2676  AGTACT               AGT^ACT
SfuI         3098  TTCGAA               TT^CGAA
SphI           33  GCATGC               GCATG^C
SspBI         162  TGTACA               T^GTACA
Tth111I      5257  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

ApaI           26  GGGCCC               GGGCC^C
             5514  GGGCCC              
AvrII        3051  CCTAGG               C^CTAGG
             5205  CCTAGG              
BlnI         3051  CCTAGG               C^CTAGG
             5205  CCTAGG              
BlpI         2350  GCTAAGC              GC^T[ACGT]AGC
             3525  GCTAAGC             
Bpu1102I     2350  GCTAAGC              GC^T[ACGT]AGC
             3525  GCTAAGC             
BsaWI         744  TCCGGT               [AT]^CCGG[AT]
             4791  ACCGGA              
BsmBI        1787  GAGACG               CGTCTC(1/5)
             5756  GAGACG              
BstEII       2242  GGTGACC              G^GT[ACGT]ACC
             4615  GGTTACC             
BstPI        2242  GGTGACC              G^GT[ACGT]ACC
             4615  GGTTACC             
CelII        2350  GCTAAGC              GC^T[ACGT]AGC
             3525  GCTAAGC             
CfoI           45  GCGC                 GCG^C
             5112  GCGC                
DraIII       4505  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             5564  CACTTGGTG           
EcoO65I      2242  GGTGACC              G^GT[ACGT]ACC
             4615  GGTTACC             
EcoT22I      5465  ATGCAT               ATGCA^T
             5717  ATGCAT              
HhaI           45  GCGC                 GCG^C
             5112  GCGC                
HinP1I         45  GCGC                 G^CGC
             5112  GCGC                
HincII       2269  GTTAAC               GT[CT]^[GA]AC
             5701  GTTAAC              
HindII       2269  GTTAAC               GT[CT]^[GA]AC
             5701  GTTAAC              
HpaI         2269  GTTAAC               GTT^AAC
             5701  GTTAAC              
MfeI         2159  CAATTG               C^AATTG
             3432  CAATTG              
MunI         2159  CAATTG               C^AATTG
             3432  CAATTG              
NdeI         2689  CATATG               CA^TATG
             4045  CATATG              
NsiI         5465  ATGCAT               ATGCA^T
             5717  ATGCAT              
SexAI         179  ACCAGGT              A^CC[AT]GGT
              321  ACCTGGT             
StuI          438  AGGCCT               AGG^CCT
             5202  AGGCCT              
XbaI         4194  TCTAGA               T^CTAGA
             6045  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AatII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApoI        
AseI        AsnI        Asp700I     AspHI       AvaII       
BamHI       BanI        BanII       BbsI        BbvI        
BclI        BfaI        BglII       BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspMI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeIII      HapII       
Hin1I       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PshBI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       XmnI        
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