Restriction map for pF1KB0048
 (Linear) (Six-base) MAPSORT of: KIBB0048.seq  Check: 9936  from: 1  to: 5268



KIBB0048.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:21  ..

AccI GT'mk_AC

Cuts at:      0    1726    2475    3471    5268 
   Size:       1726     749     996    1797

  Fragments arranged by size: 

                1797    1726     996     749
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179    3485    3848    3985    3995    4028    4199    4802 
   Size:        179    3306     363     137      10      33     171     603

Cuts at:   4802    5107    5268 
   Size:        305     161

  Fragments arranged by size: 

                3306     603     363     305     179     171     161     137
                  33      10
 
AhdI GACnn_n'nnGTC

Cuts at:      0     483     918    5139    5169    5268 
   Size:        483     435    4221      30      99

  Fragments arranged by size: 

                4221     483     435      99      30
 
AleI CACnn'nnGTG

Cuts at:      0     529    5268 
   Size:        529    4739
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3074    3106    5268 
   Size:       3074      32    2162

  Fragments arranged by size: 

                3074    2162      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     337     846    3451    5268 
   Size:        337     509    2605    1817

  Fragments arranged by size: 

                2605    1817     509     337
 
ApaI G_GGCC'C

Cuts at:      0    3512    5268 
   Size:       3512    1756
 
ApoI r'AATT_y

Cuts at:      0     593     836     998    1240    1361    2289    2811    4143 
   Size:        593     243     162     242     121     928     522    1332

Cuts at:   4143    4362    4603    5268 
   Size:        219     241     665

  Fragments arranged by size: 

                1332     928     665     593     522     243     242     241
                 219     162     121
 
AvaI C'yCGr_G

Cuts at:      0    2989    3124    4871    5268 
   Size:       2989     135    1747     397

  Fragments arranged by size: 

                2989    1747     397     135
 
BamHI G'GATC_C

Cuts at:      0    2979    3938    5268 
   Size:       2979     959    1330

  Fragments arranged by size: 

                2979    1330     959
 
BanI G'GyrC_C

Cuts at:      0    3408    5268 
   Size:       3408    1860
 
BanII G_rGCy'C

Cuts at:      0      51    1630    2840    3512    5268 
   Size:         51    1579    1210     672    1756

  Fragments arranged by size: 

                1756    1579    1210     672      51
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     553    1774    2501    4646    5197    5268 
   Size:        553    1221     727    2145     551      71

  Fragments arranged by size: 

                2145    1221     727     553     551      71
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2398    2432    3105    3139    5268 
   Size:       2398      34     673      34    2129

  Fragments arranged by size: 

                2398    2129     673      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1596    2138    5268 
   Size:       1596     542    3130

  Fragments arranged by size: 

                3130    1596     542
 
BclI T'GATC_A

Cuts at:      0     933    1044    1356    2526    5268 
   Size:        933     111     312    1170    2742

  Fragments arranged by size: 

                2742    1170     933     312     111
 
BfrBI ATG'CAT

Cuts at:      0    2628    5268 
   Size:       2628    2640
 
BglII A'GATC_T

Cuts at:      0    3787    5268 
   Size:       3787    1481
 
BlpI GC'TnA_GC

Cuts at:      0    1703    1832    5268 
   Size:       1703     129    3436

  Fragments arranged by size: 

                3436    1703     129
 
Bme1580I G_kGCm'C

Cuts at:      0    3050    3512    5268 
   Size:       3050     462    1756

  Fragments arranged by size: 

                3050    1756     462
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1633    2115    4483    5268 
   Size:       1633     482    2368     785

  Fragments arranged by size: 

                2368    1633     785     482
 
BmtI G_CTAG'C

Cuts at:      0    1714    5268 
   Size:       1714    3554
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2634    5268 
   Size:       2634    2634
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1788    2423    2729    2851    3294    3827    4511    5268 
   Size:       1788     635     306     122     443     533     684     757

  Fragments arranged by size: 

                1788     757     684     635     533     443     306     122
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    4445    5268 
   Size:       4445     823
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3138    3281    4925    5062    5268 
   Size:       3138     143    1644     137     206

  Fragments arranged by size: 

                3138    1644     206     143     137
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1745    3113    3796    5268 
   Size:       1745    1368     683    1472

  Fragments arranged by size: 

                1745    1472    1368     683
 
BsaAI yAC'GTr

Cuts at:      0    3099    5268 
   Size:       3099    2169
 
BsaBI GATnn'nnATC

Cuts at:      0    1481    1967    2984    3792    5268 
   Size:       1481     486    1017     808    1476

  Fragments arranged by size: 

                1481    1476    1017     808     486
 
BsaWI w'CCGG_w

Cuts at:      0     977    2571    3480    5268 
   Size:        977    1594     909    1788

  Fragments arranged by size: 

                1788    1594     977     909
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1244    1274    1433    1463    2634    2664    2832    2862 
   Size:       1244      30     159      30    1171      30     168      30

Cuts at:   2862    3074    3104    3292    3322    4227    4257    4509    4539 
   Size:        212      30     188      30     905      30     252      30

Cuts at:   4539    5268 
   Size:        729

  Fragments arranged by size: 

                1244    1171     905     729     252     212     188     168
                 159      30      30      30      30      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     471    2607    2848    4235    4238    5157    5268 
   Size:        471    2136     241    1387       3     919     111

  Fragments arranged by size: 

                2136    1387     919     471     241     111       3
 
BseYI C'CCAG_C

Cuts at:      0    1222    2775    2844    3488    4271    5268 
   Size:       1222    1553      69     644     783     997

  Fragments arranged by size: 

                1553    1222     997     783     644      69
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    4064    4619    5268 
   Size:       4064     555     649

  Fragments arranged by size: 

                4064     649     555
 
BsiHKAI G_wGCw'C

Cuts at:      0    3873    4326    5268 
   Size:       3873     453     942

  Fragments arranged by size: 

                3873     942     453
 
BsmI GAATG_Cn'

Cuts at:      0    2630    5268 
   Size:       2630    2638
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     815    1988    4304    5268 
   Size:        815    1173    2316     964

  Fragments arranged by size: 

                2316    1173     964     815
 
Bsp1286I G_dGCh'C

Cuts at:      0      51    1630    2840    3050    3512    3873    4326    5268 
   Size:         51    1579    1210     210     462     361     453     942

  Fragments arranged by size: 

                1579    1210     942     462     453     361     210      51
            
 
BspEI T'CCGG_A

Cuts at:      0     977    5268 
   Size:        977    4291
 
BspHI T'CATG_A

Cuts at:      0    1200    2631    5268 
   Size:       1200    1431    2637

  Fragments arranged by size: 

                2637    1431    1200
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3586    5268 
   Size:       3586    1682
 
BsrBI CCG'CTC

Cuts at:      0    1831    5268 
   Size:       1831    3437
 
BsrDI GCAATG_nn'

Cuts at:      0     197     972    3743    4085    4252    4896    5268 
   Size:        197     775    2771     342     167     644     372

  Fragments arranged by size: 

                2771     775     644     372     342     197     167
 
BsrFI r'CCGG_y

Cuts at:      0     896    5268 
   Size:        896    4372
 
BsrGI T'GTAC_A

Cuts at:      0    1610    1719    3932    5268 
   Size:       1610     109    2213    1336

  Fragments arranged by size: 

                2213    1610    1336     109
 
BssSI C'ACGA_G

Cuts at:      0    1991    5268 
   Size:       1991    3277
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    4087    5268 
   Size:       4087    1181
 
BstBI TT'CG_AA

Cuts at:      0    1644    1806    5268 
   Size:       1644     162    3462

  Fragments arranged by size: 

                3462    1644     162
 
BstYI r'GATC_y

Cuts at:      0    2955    2979    3729    3787    3938    5214    5268 
   Size:       2955      24     750      58     151    1276      54

  Fragments arranged by size: 

                2955    1276     750     151      58      54      24
 
BstZ17I GTA'TAC

Cuts at:      0    1727    5268 
   Size:       1727    3541
 
BtgI C'CryG_G

Cuts at:      0     469    3615    5070    5268 
   Size:        469    3146    1455     198

  Fragments arranged by size: 

                3146    1455     469     198
 
BtsI GCAGTG_nn'

Cuts at:      0    3691    5038    5268 
   Size:       3691    1347     230

  Fragments arranged by size: 

                3691    1347     230
 
ClaI AT'CG_AT

Cuts at:      0    4539    5268 
   Size:       4539     729
 
DraIII CAC_nnn'GTG

Cuts at:      0    4330    5116    5268 
   Size:       4330     786     152

  Fragments arranged by size: 

                4330     786     152
 
EaeI y'GGCC_r

Cuts at:      0     387     427    3271    4862    5268 
   Size:        387      40    2844    1591     406

  Fragments arranged by size: 

                2844    1591     406     387      40
 
EarI CTCTTCn'nnn_

Cuts at:      0     986    1112    3320    3356    3431    3716    4823    5268 
   Size:        986     126    2208      36      75     285    1107     445

  Fragments arranged by size: 

                2208    1107     986     445     285     126      75      36
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1763    2392    3411    5268 
   Size:       1763     629    1019    1857

  Fragments arranged by size: 

                1857    1763    1019     629
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179    1788    2423    2729    2851    3294    3485    3827 
   Size:        179    1609     635     306     122     443     191     342

Cuts at:   3827    3848    3985    3995    4028    4199    4511    4802    5107 
   Size:         21     137      10      33     171     312     291     305

Cuts at:   5107    5268 
   Size:        161

  Fragments arranged by size: 

                1609     635     443     342     312     306     305     291
                 191     179     171     161     137     122      33      21
                  10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3503    5268 
   Size:       3503    1765
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2516    3508    5268 
   Size:       2516     992    1760

  Fragments arranged by size: 

                2516    1760     992
 
EcoRI G'AATT_C

Cuts at:      0    4362    5268 
   Size:       4362     906
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1359    1432    5268 
   Size:       1359      73    3836

  Fragments arranged by size: 

                3836    1359      73
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     469     501     904     936    1667    1699    1810    1842 
   Size:        469      32     403      32     731      32     111      32

Cuts at:   1842    2178    2210    2407    2439    3292    3324    5049    5081 
   Size:        336      32     197      32     853      32    1725      32

Cuts at:   5081    5125    5155    5157    5187    5268 
   Size:         44      30       2      30      81

  Fragments arranged by size: 

                1725     853     731     469     403     336     197     111
                  81      44      32      32      32      32      32      32
                  32      32      30      30       2
 
HincII GTy'rAC

Cuts at:      0     198     405    5268 
   Size:        198     207    4863

  Fragments arranged by size: 

                4863     207     198
 
HindIII A'AGCT_T

Cuts at:      0    2735    5268 
   Size:       2735    2533
 
HpaI GTT'AAC

Cuts at:      0     198     405    5268 
   Size:        198     207    4863

  Fragments arranged by size: 

                4863     207     198
 
MfeI C'AATT_G

Cuts at:      0    2132    5268 
   Size:       2132    3136
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1272    2983    5186    5211    5268 
   Size:       1272    1711    2203      25      57

  Fragments arranged by size: 

                2203    1711    1272      57      25
 
MscI TGG'CCA

Cuts at:      0    4864    5268 
   Size:       4864     404
 
MslI CAynn'nnrTG

Cuts at:      0     529    1040    1205    5268 
   Size:        529     511     165    4063

  Fragments arranged by size: 

                4063     529     511     165
 
MspA1I CmG'CkG

Cuts at:      0     157     471     918    2352    3628    3639    4038    4822 
   Size:        157     314     447    1434    1276      11     399     784

Cuts at:   4822    5268 
   Size:        446

  Fragments arranged by size: 

                1434    1276     784     447     446     399     314     157
                  11
 
NcoI C'CATG_G

Cuts at:      0    3615    5070    5268 
   Size:       3615    1455     198

  Fragments arranged by size: 

                3615    1455     198
 
NheI G'CTAG_C

Cuts at:      0    1710    5268 
   Size:       1710    3558
 
NsiI A_TGCA'T

Cuts at:      0    2630    5268 
   Size:       2630    2638
 
NspI r_CATG'y

Cuts at:      0     229    1039    2074    2953    5268 
   Size:        229     810    1035     879    2315

  Fragments arranged by size: 

                2315    1035     879     810     229
 
PasI CC'CwG_GG

Cuts at:      0    3043    5268 
   Size:       3043    2225
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     385     967    1177    3451    4495    5268 
   Size:        385     582     210    2274    1044     773

  Fragments arranged by size: 

                2274    1044     773     582     385     210
 
PfoI T'CCnGG_A

Cuts at:      0      51     538    5208    5268 
   Size:         51     487    4670      60

  Fragments arranged by size: 

                4670     487      60      51
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3074    3106    5268 
   Size:       3074      32    2162

  Fragments arranged by size: 

                3074    2162      32
 
PspOMI G'GGCC_C

Cuts at:      0    3508    5268 
   Size:       3508    1760
 
PspXI vC'TCGA_Gb

Cuts at:      0    3124    5268 
   Size:       3124    2144
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1295    1327    2880    2912    5268 
   Size:       1295      32    1553      32    2356

  Fragments arranged by size: 

                2356    1553    1295      32      32
 
PstI C_TGCA'G

Cuts at:      0    1332    3522    3627    5268 
   Size:       1332    2190     105    1641

  Fragments arranged by size: 

                2190    1641    1332     105
 
PvuII CAG'CTG

Cuts at:      0     157    2352    3628    4038    4822    5268 
   Size:        157    2195    1276     410     784     446

  Fragments arranged by size: 

                2195    1276     784     446     410     157
 
SacII CC_GC'GG

Cuts at:      0     472    5268 
   Size:        472    4796
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3356    3431    5268 
   Size:       3356      75    1837

  Fragments arranged by size: 

                3356    1837      75
 
ScaI AGT'ACT

Cuts at:      0    2898    5268 
   Size:       2898    2370
 
SfcI C'TryA_G

Cuts at:      0     169    1328    3518    3623    5268 
   Size:        169    1159    2190     105    1645

  Fragments arranged by size: 

                2190    1645    1159     169     105
 
SmlI C'TyrA_G

Cuts at:      0    3124    3153    3296    4940    5077    5268 
   Size:       3124      29     143    1644     137     191

  Fragments arranged by size: 

                3124    1644     191     143     137      29
 
SnaBI TAC'GTA

Cuts at:      0    3099    5268 
   Size:       3099    2169
 
SphI G_CATG'C

Cuts at:      0    1039    5268 
   Size:       1039    4229
 
SspI AAT'ATT

Cuts at:      0     630    1298    3547    4024    5268 
   Size:        630     668    2249     477    1244

  Fragments arranged by size: 

                2249    1244     668     630     477
 
StuI AGG'CCT

Cuts at:      0     882    1899    5268 
   Size:        882    1017    3369

  Fragments arranged by size: 

                3369    1017     882
 
StyI C'CwwG_G

Cuts at:      0    1334    3576    3615    5070    5268 
   Size:       1334    2242      39    1455     198

  Fragments arranged by size: 

                2242    1455    1334     198      39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     197    3056    3977    4067    5268 
   Size:        197    2859     921      90    1201

  Fragments arranged by size: 

                2859    1201     921     197      90
 
TatI w'GTAC_w

Cuts at:      0    1610    1719    2896    3753    3932    5268 
   Size:       1610     109    1177     857     179    1336

  Fragments arranged by size: 

                1610    1336    1177     857     179     109
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1244    1276    1433    1465    2832    2864    4227    4259 
   Size:       1244      32     157      32    1367      32    1363      32

Cuts at:   4259    5104    5136    5268 
   Size:        845      32     132

  Fragments arranged by size: 

                1367    1363    1244     845     157     132      32      32
                  32      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1314    3589    5268 
   Size:       1314    2275    1679

  Fragments arranged by size: 

                2275    1679    1314
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1230    1941    2017    5268 
   Size:       1230     711      76    3251

  Fragments arranged by size: 

                3251    1230     711      76
 
XhoI C'TCGA_G

Cuts at:      0    3124    5268 
   Size:       3124    2144
 
XmnI GAAnn'nnTTC

Cuts at:      0    2330    3907    5268 
   Size:       2330    1577    1361

  Fragments arranged by size: 

                2330    1577    1361
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     AleI     AloI    AlwNI     ApaI     ApoI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstYI  BstZ17I     BtgI     BtsI     ClaI   DraIII     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I   HincII
  HindIII     HpaI     MfeI     MmeI     MscI     MslI   MspA1I     NcoI
     NheI     NsiI     NspI     PasI    PflMI     PfoI     PpiI   PspOMI
    PspXI     PsrI     PstI    PvuII    SacII     SapI     ScaI     SfcI
     SmlI    SnaBI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
    BbvCI     BglI    BmgBI    BsaHI    BsiEI    BsiWI   BssHII   BstEII
    BstXI   Bsu36I    BtgZI    CspCI     DraI     DrdI     EagI  EcoICRI
    EcoRV     FseI     FspI    FspAI    HaeII     KasI     KpnI     MluI
     NaeI     NarI     NdeI   NgoMIV     NotI     NruI     PacI     PciI
     PmeI     PmlI    PpuMI    PshAI     PsiI     PvuI    RsrII     SacI
     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SpeI     SrfI     SwaI  Tth111I     XmaI     ZraI
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