Restriction map for pF1KB0020
##################################
# NO CUT ENZYMES (   51 enzymes) #
##################################

AhdI        AscI        AseI        AsnI        AspEI       
AviII       BbvCI       BglII       BsaBI       BsiWI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstZ17I     ClaI        CpoI        
DraIII      Eam1105I    EcoRI       EcoT22I     FseI        
FspI        MamI        MluI        NcoI        NheI        
NotI        NruI        NsiI        NspI        PacI        
PaeR7I      PmeI        PshBI       PvuI        RcaI        
RsrII       SalI        ScaI        SexAI       SfiI        
SphI        SspBI       SspI        SwaI        XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AatII        4719  GACGTC               GACGT^C
Acc65I       3607  GGTACC               G^GTACC
AccI         1003  GTAGAC               GT^[AC][GT]AC
AccII         123  CGCG                 CG^CG
AclI         4326  AACGTT               AA^CGTT
AflII        4485  CTTAAG               C^TTAAG
AgeI         3175  ACCGGT               A^CCGGT
Aor51HI      1918  AGCGCT               AGC^GCT
ApaI         4426  GGGCCC               GGGCC^C
Asp700I       439  GAACAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      3607  GGTACC               G^GTACC
AvrII        4197  CCTAGG               C^CTAGG
BamHI        2180  GGATCC               G^GATCC
BclI         4959  TGATCA               T^GATCA
BfrI         4485  CTTAAG               C^TTAAG
BlnI         4197  CCTAGG               C^CTAGG
BsmI          790  GAATGC               GAATGC(1/-1)
BsrBI        4394  CCGCTC               CCGCTC(-3/-3)
BsrDI        2116  GCAATG               GCAATG(2/0)
BssSI        1096  CACGAG               CACGAG(-5/-1)
BstBI        3841  TTCGAA               TT^CGAA
BstEII        235  GGTGACC              G^GT[ACGT]ACC
BstPI         235  GGTGACC              G^GT[ACGT]ACC
BstUI         123  CGCG                 CG^CG
BstYI        2180  GGATCC               [GA]^GATC[CT]
DrdI         2518  GACTCTGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1918  AGCGCT               AGC^GCT
EcoO65I       235  GGTGACC              G^GT[ACGT]ACC
EcoRV        2739  GATATC               GAT^ATC
FbaI         4959  TGATCA               T^GATCA
KpnI         3607  GGTACC               GGTAC^C
KspI          122  CCGCGG               CCGC^GG
MfeI         4586  CAATTG               C^AATTG
MflI         2180  GGATCC               [GA]^GATC[CT]
MunI         4586  CAATTG               C^AATTG
MvnI          123  CGCG                 CG^CG
NdeI         2668  CATATG               CA^TATG
NspV         3841  TTCGAA               TT^CGAA
PinAI        3175  ACCGGT               A^CCGGT
PshAI        1441  GACCTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     4326  AACGTT               AA^CGTT
SacII         122  CCGCGG               CCGC^GG
SbfI         4336  CCTGCAGG             CCTGCA^GG
SfuI         3841  TTCGAA               TT^CGAA
SgrAI        3174  CACCGGTG             C[GA]^CCGG[CT]G
SmaI         2879  CCCGGG               CCC^GGG
SnaBI        4688  TACGTA               TAC^GTA
Sse8387I     4336  CCTGCAGG             CCTGCA^GG
XbaI         3726  TCTAGA               T^CTAGA
XhoII        2180  GGATCC               [GA]^GATC[CT]
XmaCI        2879  CCCGGG               C^CCGGG
XmaI         2879  CCCGGG               C^CCGGG
XmnI          439  GAACAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccIII       1414  TCCGGA               T^CCGGA
             1662  TCCGGA              
AflIII       1405  ACGTGT               A^C[GA][CT]GT
             1729  ACGTGT              
Alw44I       4343  GTGCAC               G^TGCAC
             5254  GTGCAC              
ApaLI        4343  GTGCAC               G^TGCAC
             5254  GTGCAC              
BalI          246  TGGCCA               TGG^CCA
              774  TGGCCA              
BbeI            4  GGCGCC               GGCGC^C
              108  GGCGCC              
BbrPI        1728  CACGTG               CAC^GTG
             4744  CACGTG              
BcgI         1689  CGAGAGCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2973  CGAAGCATCTGC        
BciVI        2019  GGATAC               GTATCC(6/5)
             3763  GTATCC              
BglI          100  GCCGAGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4419  GCCCACAGGGC         
BseAI        1414  TCCGGA               T^CCGGA
             1662  TCCGGA              
BspEI        1414  TCCGGA               T^CCGGA
             1662  TCCGGA              
DraI          541  TTTAAA               TTT^AAA
             2284  TTTAAA              
EagI           98  CGGCCG               C^GGCCG
             2531  CGGCCG              
EclXI          98  CGGCCG               C^GGCCG
             2531  CGGCCG              
Eco52I         98  CGGCCG               C^GGCCG
             2531  CGGCCG              
EcoNI         225  CCTCCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2248  CCTACCGAAGG         
FauI          130  CCCGC                CCCGC(4/6)
              151  CCCGC               
HpaI         2793  GTTAAC               GTT^AAC
             3024  GTTAAC              
KasI            4  GGCGCC               G^GCGCC
              108  GGCGCC              
MluNI         246  TGGCCA               TGG^CCA
              774  TGGCCA              
MroI         1414  TCCGGA               T^CCGGA
             1662  TCCGGA              
MscI          246  TGGCCA               TGG^CCA
              774  TGGCCA              
NarI            4  GGCGCC               GG^CGCC
              108  GGCGCC              
PflMI         777  CCAAGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3129  CCAGGCAGTGG         
PmaCI        1728  CACGTG               CAC^GTG
             4744  CACGTG              
PmlI         1728  CACGTG               CAC^GTG
             4744  CACGTG              
SfoI            4  GGCGCC               GGC^GCC
              108  GGCGCC              
SpeI          853  ACTAGT               A^CTAGT
             5076  ACTAGT              
StuI         3658  AGGCCT               AGG^CCT
             4808  AGGCCT              
Van91I        777  CCAAGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3129  CCAGGCAGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        AspHI       
AspI        AvaI        AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrFI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstXI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       PflFI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com