Restriction map for pF1KB0001
##################################
# NO CUT ENZYMES (   55 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AhdI        
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       AvrII       BfrI        BglII       BlnI        
BsiWI       BsmI        BspDI       BspLU11I    Bst1107I    
BstBI       BstZ17I     ClaI        DraI        Eam1105I    
EcoRI       EcoT22I     FspI        HindIII     HpaI        
KpnI        MfeI        MseI        MunI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
Psp1406I    SalI        ScaI        SfuI        SpeI        
SspI        SwaI        Tru9I       XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AatII        5968  GACGTC               GACGT^C
AcsI         5311  AAATTC               [GA]^AATT[CT]
ApoI         5311  AAATTC               [GA]^AATT[CT]
BbrPI        2251  CACGTG               CAC^GTG
BciVI        7414  GTATCC               GTATCC(6/5)
BclI         7208  TGATCA               T^GATCA
BsaBI        3105  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I     3197  TGTACA               T^GTACA
BspHI        6470  TCATGA               T^CATGA
BsrGI        3197  TGTACA               T^GTACA
EcoRV         799  GATATC               GAT^ATC
FbaI         7208  TGATCA               T^GATCA
FseI         3738  GGCCGGCC             GGCCGG^CC
KspI         3307  CCGCGG               CCGC^GG
MamI         3105  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI         7101  CATATG               CA^TATG
PmaCI        2251  CACGTG               CAC^GTG
PmlI         2251  CACGTG               CAC^GTG
PvuI         6233  CGATCG               CGAT^CG
RcaI         6470  TCATGA               T^CATGA
SacII        3307  CCGCGG               CCGC^GG
SgrAI        5788  CGCCGGTG             C[GA]^CCGG[CT]G
SnaBI        7027  TACGTA               TAC^GTA
SspBI        3197  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccIII       5759  TCCGGA               T^CCGGA
             6551  TCCGGA              
AflIII       4124  ACGCGT               A^C[GA][CT]GT
             7512  ACGCGT              
Aor51HI      1101  AGCGCT               AGC^GCT
             1895  AGCGCT              
Asp700I      1581  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4176  GAAGTCCTTC          
BamHI        3891  GGATCC               G^GATCC
             5756  GGATCC              
BsaAI        2251  CACGTG               [CT]AC^GT[GA]
             7027  TACGTA              
BseAI        5759  TCCGGA               T^CCGGA
             6551  TCCGGA              
BspEI        5759  TCCGGA               T^CCGGA
             6551  TCCGGA              
BsrDI        5865  CATTGC               GCAATG(2/0)
             6080  GCAATG              
BssHII       3390  GCGCGC               G^CGCGC
             7338  GCGCGC              
BssSI         379  CTCGTG               CACGAG(-5/-1)
             5734  CTCGTG              
CpoI          148  CGGTCCG              CG^G[AT]CCG
             2026  CGGACCG             
DraIII       1500  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2263  CACGCGGTG           
EagI          836  CGGCCG               C^GGCCG
             2534  CGGCCG              
EclXI         836  CGGCCG               C^GGCCG
             2534  CGGCCG              
Eco47III     1101  AGCGCT               AGC^GCT
             1895  AGCGCT              
Eco52I        836  CGGCCG               C^GGCCG
             2534  CGGCCG              
MluI         4124  ACGCGT               A^CGCGT
             7512  ACGCGT              
MroI         5759  TCCGGA               T^CCGGA
             6551  TCCGGA              
RsrII         148  CGGTCCG              CG^G[AT]CCG
             2026  CGGACCG             
SapI         2901  GCTCTTC              GCTCTTC(1/4)
             4197  GCTCTTC             
SbfI         3399  CCTGCAGG             CCTGCA^GG
             3554  CCTGCAGG            
SphI         1355  GCATGC               GCATG^C
             6601  GCATGC              
Sse8387I     3399  CCTGCAGG             CCTGCA^GG
             3554  CCTGCAGG            
TfiI          292  GATTC                G^A[AT]TC
             1552  GATTC               
XmnI         1581  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4176  GAAGTCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  153 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        Alw44I      AlwI        AlwNI       AocI        
ApaI        ApaLI       AspHI       AspI        AvaI        
AvaII       BalI        BanI        BanII       BbeI        
BbsI        BbvCI       BbvI        BcgI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DrdI        
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflFI       PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SfiI        SfoI        SmaI        SmlI        StuI        
StyI        TaqI        TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI        
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