Restriction map for pF1KB0001
 (Linear) (Six-base) MAPSORT of: KIBB0001.seq  Check: 1415  from: 1  to: 7533



KIBB0001.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     640    1113    1440    3233    3318    3588    4678    5553 
   Size:        640     473     327    1793      85     270    1090     875

Cuts at:   5553    5808    6873    7533 
   Size:        255    1065     660

  Fragments arranged by size: 

                1793    1090    1065     875     660     640     473     327
                 270     255      85
 
AatII G_ACGT'C

Cuts at:      0    5972    7533 
   Size:       5972    1561
 
AccI GT'mk_AC

Cuts at:      0    4514    4697    4952    7533 
   Size:       4514     183     255    2581

  Fragments arranged by size: 

                4514    2581     255     183
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     432     595    1615    2332    2344    2984    3490 
   Size:        181     251     163    1020     717      12     640     506

Cuts at:   3490    3547    3733    4192    4303    4348    5377    5614    7189 
   Size:         57     186     459     111      45    1029     237    1575

Cuts at:   7189    7533 
   Size:        344

  Fragments arranged by size: 

                1575    1029    1020     717     640     506     459     344
                 251     237     186     181     163     111      57      45
                  12
 
AfeI AGC'GCT

Cuts at:      0    1103    1897    7533 
   Size:       1103     794    5636

  Fragments arranged by size: 

                5636    1103     794
 
AflIII A'CryG_T

Cuts at:      0    4124    7512    7533 
   Size:       4124    3388      21

  Fragments arranged by size: 

                4124    3388      21
 
AleI CACnn'nnGTG

Cuts at:      0     589     892    3691    5092    6763    7533 
   Size:        589     303    2799    1401    1671     770

  Fragments arranged by size: 

                2799    1671    1401     770     589     303
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     557    3890    5585    6343    7345    7533 
   Size:        557    3333    1695     758    1002     188

  Fragments arranged by size: 

                3333    1695    1002     758     557     188
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    6643    6675    7533 
   Size:       6643      32     858

  Fragments arranged by size: 

                6643     858      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     912    1140    1308    1341    1611    3195    3441    3468 
   Size:        912     228     168      33     270    1584     246      27

Cuts at:   3468    3531    3603    3837    3904    5709    6495    6567    7230 
   Size:         63      72     234      67    1805     786      72     663

Cuts at:   7230    7245    7533 
   Size:         15     288

  Fragments arranged by size: 

                1805    1584     912     786     663     288     270     246
                 234     228     168      72      72      67      63      33
                  27      15
 
ApaI G_GGCC'C

Cuts at:      0     458    1062    3501    5502    5856    5891    6685    6901 
   Size:        458     604    2439    2001     354      35     794     216

Cuts at:   6901    7533 
   Size:        632

  Fragments arranged by size: 

                2439    2001     794     632     604     458     354     216
                  35
 
ApaLI G'TGCA_C

Cuts at:      0    1198    5481    6739    6816    7533 
   Size:       1198    4283    1258      77     717

  Fragments arranged by size: 

                4283    1258    1198     717      77
 
ApoI r'AATT_y

Cuts at:      0    5311    7533 
   Size:       5311    2222
 
AvaI C'yCGr_G

Cuts at:      0     370     622     926    1005    1905    1953    2152    2471 
   Size:        370     252     304      79     900      48     199     319

Cuts at:   2471    3048    3509    3964    4057    4350    4461    4610    4809 
   Size:        577     461     455      93     293     111     149     199

Cuts at:   4809    4999    5022    5028    5168    5855    6017    6821    6892 
   Size:        190      23       6     140     687     162     804      71

Cuts at:   6892    7533 
   Size:        641

  Fragments arranged by size: 

                 900     804     687     641     577     461     455     370
                 319     304     293     252     199     199     190     162
                 149     140     111      93      79      71      48      23
                   6
 
BamHI G'GATC_C

Cuts at:      0    3891    5756    7533 
   Size:       3891    1865    1777

  Fragments arranged by size: 

                3891    1865    1777
 
BanI G'GyrC_C

Cuts at:      0     628     748     886     989    1452    1852    1914    3330 
   Size:        628     120     138     103     463     400      62    1416

Cuts at:   3330    3377    3480    4049    4678    4848    4941    5798    5992 
   Size:         47     103     569     629     170      93     857     194

Cuts at:   5992    6181    7354    7533 
   Size:        189    1173     179

  Fragments arranged by size: 

                1416    1173     857     629     628     569     463     400
                 194     189     179     170     138     120     103     103
                  93      62      47
 
BanII G_rGCy'C

Cuts at:      0     131     458     933    1055    1062    1192    2093    2224 
   Size:        131     327     475     122       7     130     901     131

Cuts at:   2224    2945    3121    3290    3501    4391    4468    5029    5502 
   Size:        721     176     169     211     890      77     561     473

Cuts at:   5502    5856    5891    6509    6685    6718    6757    6901    6979 
   Size:        354      35     618     176      33      39     144      78

Cuts at:   6979    7533 
   Size:        554

  Fragments arranged by size: 

                 901     890     721     618     561     554     475     473
                 354     327     211     176     176     169     144     131
                 131     130     122      78      77      39      35      33
                   7
 
BbeI G_GCGC'C

Cuts at:      0    1856    4682    5802    6185    7358    7533 
   Size:       1856    2826    1120     383    1173     175

  Fragments arranged by size: 

                2826    1856    1173    1120     383     175
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2695    4310    5076    5895    6045    7533 
   Size:       2695    1615     766     819     150    1488

  Fragments arranged by size: 

                2695    1615    1488     819     766     150
 
BbvCI CC'TCA_GC

Cuts at:      0     230    1714    1993    2571    3250    3978    4429    7082 
   Size:        230    1484     279     578     679     728     451    2653

Cuts at:   7082    7533 
   Size:        451

  Fragments arranged by size: 

                2653    1484     728     679     578     451     451     279
                 230
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     624     658     917     951    5139    5173    5392    5426 
   Size:        624      34     259      34    4188      34     219      34

Cuts at:   5426    5538    5572    6008    6042    6403    6437    7533 
   Size:        112      34     436      34     361      34    1096

  Fragments arranged by size: 

                4188    1096     624     436     361     259     219     112
                  34      34      34      34      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    7425    7533 
   Size:       7425     108
 
BclI T'GATC_A

Cuts at:      0    7208    7533 
   Size:       7208     325
 
BglI GCCn_nnn'nGGC

Cuts at:      0     517     626     844    1907    3223    3841    4166    4323 
   Size:        517     109     218    1063    1316     618     325     157

Cuts at:   4323    4534    5875    6161    6937    7226    7533 
   Size:        211    1341     286     776     289     307

  Fragments arranged by size: 

                1341    1316    1063     776     618     517     325     307
                 289     286     218     211     157     109
 
BlpI GC'TnA_GC

Cuts at:      0     616    1522    1615    1699    2137    3643    3778    3802 
   Size:        616     906      93      84     438    1506     135      24

Cuts at:   3802    6790    7246    7289    7505    7533 
   Size:       2988     456      43     216      28

  Fragments arranged by size: 

                2988    1506     906     616     456     438     216     135
                  93      84      43      28      24
 
Bme1580I G_kGCm'C

Cuts at:      0     458     541     631     992    1062    1202    1457    1501 
   Size:        458      83      90     361      70     140     255      44

Cuts at:   1501    1919    3335    3485    3501    4052    5485    5502    5856 
   Size:        418    1416     150      16     551    1433      17     354

Cuts at:   5856    5891    6031    6685    6743    6820    6901    7533 
   Size:         35     140     654      58      77      81     632

  Fragments arranged by size: 

                1433    1416     654     632     551     458     418     361
                 354     255     150     140     140      90      83      81
                  77      70      58      44      35      17      16
 
BmgBI CAC'GTC

Cuts at:      0    2186    2529    3231    4446    6195    7425    7533 
   Size:       2186     343     702    1215    1749    1230     108

  Fragments arranged by size: 

                2186    1749    1230    1215     702     343     108
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2154    2792    3084    7533 
   Size:       2154     638     292    4449

  Fragments arranged by size: 

                4449    2154     638     292
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1020    4953    6588    7533 
   Size:       1020    3933    1635     945

  Fragments arranged by size: 

                3933    1635    1020     945
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     471    1063    1333    1833    2296    2398    2818    2833 
   Size:        471     592     270     500     463     102     420      15

Cuts at:   2833    3046    3361    3607    3859    4128    4576    4945    5107 
   Size:        213     315     246     252     269     448     369     162

Cuts at:   5107    5713    5866    5959    6295    6517    7171    7474    7489 
   Size:        606     153      93     336     222     654     303      15

Cuts at:   7489    7533 
   Size:         44

  Fragments arranged by size: 

                 654     606     592     500     471     463     448     420
                 369     336     315     303     270     269     252     246
                 222     213     162     153     102      93      44      15
                  15
 
Bpu10I CC'TnA_GC

Cuts at:      0     230     358    1351    1714    1993    2258    2571    2674 
   Size:        230     128     993     363     279     265     313     103

Cuts at:   2674    3250    3978    4114    4429    6032    6316    7082    7533 
   Size:        576     728     136     315    1603     284     766     451

  Fragments arranged by size: 

                1603     993     766     728     576     451     363     315
                 313     284     279     265     230     136     128     103
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     621    2077    3655    5461    5943    7533 
   Size:        621    1456    1578    1806     482    1590

  Fragments arranged by size: 

                1806    1590    1578    1456     621     482
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     337     359    4490    7533 
   Size:        337      22    4131    3043

  Fragments arranged by size: 

                4131    3043     337      22
 
BsaAI yAC'GTr

Cuts at:      0    2253    7029    7533 
   Size:       2253    4776     504

  Fragments arranged by size: 

                4776    2253     504
 
BsaBI GATnn'nnATC

Cuts at:      0    3109    7533 
   Size:       3109    4424
 
BsaHI Gr'CG_yC

Cuts at:      0     317    1853    2426    2717    3272    4679    4820    5513 
   Size:        317    1536     573     291     555    1407     141     693

Cuts at:   5513    5799    5900    5969    6182    6265    7355    7533 
   Size:        286     101      69     213      83    1090     178

  Fragments arranged by size: 

                1536    1407    1090     693     573     555     317     291
                 286     213     178     141     101      83      69
 
BsaWI w'CCGG_w

Cuts at:      0    4406    4887    5759    6551    7533 
   Size:       4406     481     872     792     982

  Fragments arranged by size: 

                4406     982     872     792     481
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     415     445    1993    2023    2230    2260    2584    2614 
   Size:        415      30    1548      30     207      30     324      30

Cuts at:   2614    3361    3391    4450    4480    4487    4517    4609    4639 
   Size:        747      30    1059      30       7      30      92      30

Cuts at:   4639    4690    4720    6533    6563    6643    6673    7375    7405 
   Size:         51      30    1813      30      80      30     702      30

Cuts at:   7405    7533 
   Size:        128

  Fragments arranged by size: 

                1813    1548    1059     747     702     415     324     207
                 128      92      80      51      30      30      30      30
                  30      30      30      30      30      30      30      30
                   7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     474    1804    1897    3385    3421    4573    4588 
   Size:         19     455    1330      93    1488      36    1152      15

Cuts at:   4588    4635    4942    5590    6048    7195    7533 
   Size:         47     307     648     458    1147     338

  Fragments arranged by size: 

                1488    1330    1152    1147     648     458     455     338
                 307      93      47      36      19      15
 
BseYI C'CCAG_C

Cuts at:      0     457     606    1098    1416    2463    3458    3706    3910 
   Size:        457     149     492     318    1047     995     248     204

Cuts at:   3910    4268    4634    4661    4682    5138    6254    6632    6956 
   Size:        358     366      27      21     456    1116     378     324

Cuts at:   6956    6968    6978    7533 
   Size:         12      10     555

  Fragments arranged by size: 

                1116    1047     995     555     492     457     456     378
                 366     358     324     318     248     204     149      27
                  21      12      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     613    1054    1321    1465    2319    2392    2740    3454 
   Size:        613     441     267     144     854      73     348     714

Cuts at:   3454    4015    4674    5461    5581    5746    5836    6193    6730 
   Size:        561     659     787     120     165      90     357     537

Cuts at:   6730    6907    7135    7533 
   Size:        177     228     398

  Fragments arranged by size: 

                 854     787     714     659     613     561     537     441
                 398     357     348     267     228     177     165     144
                 120      90      73
 
BsiEI CG_ry'CG

Cuts at:      0     839    2537    2646    3822    5292    6236    7533 
   Size:        839    1698     109    1176    1470     944    1297

  Fragments arranged by size: 

                1698    1470    1297    1176     944     839     109
 
BsiHKAI G_wGCw'C

Cuts at:      0    1049    1202    1621    1633    2093    2264    4603    5485 
   Size:       1049     153     419      12     460     171    2339     882

Cuts at:   5485    6223    6509    6718    6743    6820    6955    7139    7533 
   Size:        738     286     209      25      77     135     184     394

  Fragments arranged by size: 

                2339    1049     882     738     460     419     394     286
                 209     184     171     153     135      77      25      12
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    4811    4999    6205    7084    7533 
   Size:       4811     188    1206     879     449

  Fragments arranged by size: 

                4811    1206     879     449     188
 
Bsp1286I G_dGCh'C

Cuts at:      0     131     458     541     631     933     992    1049    1055 
   Size:        131     327      83      90     302      59      57       6

Cuts at:   1055    1062    1192    1202    1457    1501    1621    1633    1919 
   Size:          7     130      10     255      44     120      12     286

Cuts at:   1919    2093    2224    2264    2945    3121    3290    3335    3485 
   Size:        174     131      40     681     176     169      45     150

Cuts at:   3485    3501    4052    4391    4468    4603    5029    5485    5502 
   Size:         16     551     339      77     135     426     456      17

Cuts at:   5502    5856    5891    6031    6223    6509    6685    6718    6743 
   Size:        354      35     140     192     286     176      33      25

Cuts at:   6743    6757    6820    6901    6955    6979    7139    7533 
   Size:         14      63      81      54      24     160     394

  Fragments arranged by size: 

                 681     551     456     426     394     354     339     327
                 302     286     286     255     192     176     176     174
                 169     160     150     140     135     131     131     130
                 120      90      83      81      77      63      59      57
                  54      45      44      40      35      33      25      24
                  17      16      14      12      10       7       6
 
BspEI T'CCGG_A

Cuts at:      0    5759    6551    7533 
   Size:       5759     792     982

  Fragments arranged by size: 

                5759     982     792
 
BspHI T'CATG_A

Cuts at:      0    6470    7533 
   Size:       6470    1063
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     640    1076    1113    1440    1703    3149    3233    3318 
   Size:        640     436      37     327     263    1446      84      85

Cuts at:   3318    3587    3588    4678    5553    5772    5808    6662    6873 
   Size:        269       1    1090     875     219      36     854     211

Cuts at:   6873    7533 
   Size:        660

  Fragments arranged by size: 

                1446    1090     875     854     660     640     436     327
                 269     263     219     211      85      84      37      36
                   1
 
BsrBI CCG'CTC

Cuts at:      0     335     671     821    1473    5337    6723    7533 
   Size:        335     336     150     652    3864    1386     810

  Fragments arranged by size: 

                3864    1386     810     652     336     335     150
 
BsrDI GCAATG_nn'

Cuts at:      0    5864    6087    7533 
   Size:       5864     223    1446

  Fragments arranged by size: 

                5864    1446     223
 
BsrFI r'CCGG_y

Cuts at:      0      19     425     745    2029    2392    2532    3739    4211 
   Size:         19     406     320    1284     363     140    1207     472

Cuts at:   4211    4245    5446    5789    6143    6913    7533 
   Size:         34    1201     343     354     770     620

  Fragments arranged by size: 

                1284    1207    1201     770     620     472     406     363
                 354     343     320     140      34      19
 
BsrGI T'GTAC_A

Cuts at:      0    3197    7533 
   Size:       3197    4336
 
BssHII G'CGCG_C

Cuts at:      0    3390    7338    7533 
   Size:       3390    3948     195

  Fragments arranged by size: 

                3948    3390     195
 
BssSI C'ACGA_G

Cuts at:      0     379    5734    7533 
   Size:        379    5355    1799

  Fragments arranged by size: 

                5355    1799     379
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1308    1452    3330    3441    3531    3904    4038    5193 
   Size:       1308     144    1878     111      90     373     134    1155

Cuts at:   5193    7533 
   Size:       2340

  Fragments arranged by size: 

                2340    1878    1308    1155     373     144     134     111
                  90
 
BstEII G'GTnAC_C

Cuts at:      0    3453    3748    6012    7435    7533 
   Size:       3453     295    2264    1423      98

  Fragments arranged by size: 

                3453    2264    1423     295      98
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     495    3918    4772    4863    6597    7533 
   Size:        495    3423     854      91    1734     936

  Fragments arranged by size: 

                3423    1734     936     854     495      91
 
BstYI r'GATC_y

Cuts at:      0    1094    3503    3891    5756    7533 
   Size:       1094    2409     388    1865    1777

  Fragments arranged by size: 

                2409    1865    1777    1094     388
 
Bsu36I CC'TnA_GG

Cuts at:      0     792    1210    1292    1961    4571    7533 
   Size:        792     418      82     669    2610    2962

  Fragments arranged by size: 

                2962    2610     792     669     418      82
 
BtgI C'CryG_G

Cuts at:      0     876    1149    1401    1856    1865    2936    3307    3692 
   Size:        876     273     252     455       9    1071     371     385

Cuts at:   3692    3744    5093    5373    5493    5891    5915    6119    6389 
   Size:         52    1349     280     120     398      24     204     270

Cuts at:   6389    7199    7317    7533 
   Size:        810     118     216

  Fragments arranged by size: 

                1349    1071     876     810     455     398     385     371
                 280     273     270     252     216     204     120     118
                  52      24       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     427     447    1353    2052    5037    5043    5649    7533 
   Size:        427      20     906     699    2985       6     606    1884

  Fragments arranged by size: 

                2985    1884     906     699     606     427      20       6
            
 
BtsI GCAGTG_nn'

Cuts at:      0     541    2077    3202    3439    3686    4036    5415    5594 
   Size:        541    1536    1125     237     247     350    1379     179

Cuts at:   5594    5710    6181    6820    7533 
   Size:        116     471     639     713

  Fragments arranged by size: 

                1536    1379    1125     713     639     541     471     350
                 247     237     179     116
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    7332    7367    7533 
   Size:       7332      35     166

  Fragments arranged by size: 

                7332     166      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1505    2268    7533 
   Size:       1505     763    5265

  Fragments arranged by size: 

                5265    1505     763
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     705    2647    6235    7533 
   Size:        705    1942    3588    1298

  Fragments arranged by size: 

                3588    1942    1298     705
 
EaeI y'GGCC_r

Cuts at:      0     419     686     836    1022    1734    2390    2534    3122 
   Size:        419     267     150     186     712     656     144     588

Cuts at:   3122    3263    3737    3741    3812    4101    4733    4865    5621 
   Size:        141     474       4      71     289     632     132     756

Cuts at:   5621    5912    6167    6911    7533 
   Size:        291     255     744     622

  Fragments arranged by size: 

                 756     744     712     656     632     622     588     474
                 419     291     289     267     255     186     150     144
                 141     132      71       4
 
EagI C'GGCC_G

Cuts at:      0     836    2534    7533 
   Size:        836    1698    4999

  Fragments arranged by size: 

                4999    1698     836
 
EarI CTCTTCn'nnn_

Cuts at:      0    2659    2908    2953    4144    4204    4314    5243    6295 
   Size:       2659     249      45    1191      60     110     929    1052

Cuts at:   6295    7006    7533 
   Size:        711     527

  Fragments arranged by size: 

                2659    1191    1052     929     711     527     249     110
                  60      45
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2181    4369    4531    5260    5776    6105    6463    6595 
   Size:       2181    2188     162     729     516     329     358     132

Cuts at:   6595    7533 
   Size:        938

  Fragments arranged by size: 

                2188    2181     938     729     516     358     329     162
                 132
 
EcoICRI GAG'CTC

Cuts at:      0    2091    6507    6716    7533 
   Size:       2091    4416     209     817

  Fragments arranged by size: 

                4416    2091     817     209
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     432     471     595    1063    1333    1615    1833 
   Size:        181     251      39     124     468     270     282     218

Cuts at:   1833    2296    2332    2344    2398    2818    2833    2984    3046 
   Size:        463      36      12      54     420      15     151      62

Cuts at:   3046    3361    3490    3547    3607    3733    3859    4128    4192 
   Size:        315     129      57      60     126     126     269      64

Cuts at:   4192    4303    4348    4576    4945    5107    5377    5614    5713 
   Size:        111      45     228     369     162     270     237      99

Cuts at:   5713    5866    5959    6295    6517    7171    7189    7474    7489 
   Size:        153      93     336     222     654      18     285      15

Cuts at:   7489    7533 
   Size:         44

  Fragments arranged by size: 

                 654     468     463     420     369     336     315     285
                 282     270     270     269     251     237     228     222
                 218     181     162     153     151     129     126     126
                 124     111      99      93      64      62      60      57
                  54      45      44      39      36      18      15      15
                  12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1024    1747    1783    1812    2299    2956    3403    4117 
   Size:       1024     723      36      29     487     657     447     714

Cuts at:   4117    4555    6382    7393    7533 
   Size:        438    1827    1011     140

  Fragments arranged by size: 

                1827    1024    1011     723     714     657     487     447
                 438     140      36      29
 
EcoO109I rG'GnC_Cy

Cuts at:      0     165     354     454    1059    1320    1338    1957    1965 
   Size:        165     189     100     605     261      18     619       8

Cuts at:   1965    2241    3497    3513    3633    3834    3969    4526    4617 
   Size:        276    1256      16     120     201     135     557      91

Cuts at:   4617    4860    5379    5498    5852    5859    6375    6681    6682 
   Size:        243     519     119     354       7     516     306       1

Cuts at:   6682    6897    6898    6929    7173    7227    7368    7533 
   Size:        215       1      31     244      54     141     165

  Fragments arranged by size: 

                1256     619     605     557     519     516     354     306
                 276     261     244     243     215     201     189     165
                 165     141     135     120     119     100      91      54
                  31      18      16       8       7       1       1
 
EcoRV GAT'ATC

Cuts at:      0     801    7533 
   Size:        801    6732
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3369    4197    7533 
   Size:       3369     828    3336

  Fragments arranged by size: 

                3369    3336     828
 
FseI GG_CCGG'CC

Cuts at:      0    3743    7533 
   Size:       3743    3790
 
HaeII r_GCGC'y

Cuts at:      0    1105    1470    1856    1899    3917    3929    4682    5437 
   Size:       1105     365     386      43    2018      12     753     755

Cuts at:   5437    5802    6185    7159    7358    7533 
   Size:        365     383     974     199     175

  Fragments arranged by size: 

                2018    1105     974     755     753     386     383     365
                 365     199     175      43      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     213     245    1541    1573    1811    1843    2584    2616 
   Size:        213      32    1296      32     238      32     741      32

Cuts at:   2616    3961    3993    4291    4323    4450    4482    4609    4641 
   Size:       1345      32     298      32     127      32     127      32

Cuts at:   4641    7533 
   Size:       2892

  Fragments arranged by size: 

                2892    1345    1296     741     298     238     213     127
                 127      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    2208    5223    5741    6429    7533 
   Size:       2208    3015     518     688    1104

  Fragments arranged by size: 

                3015    2208    1104     688     518
 
KasI G'GCGC_C

Cuts at:      0    1852    4678    5798    6181    7354    7533 
   Size:       1852    2826    1120     383    1173     179

  Fragments arranged by size: 

                2826    1852    1173    1120     383     179
 
MluI A'CGCG_T

Cuts at:      0    4124    7512    7533 
   Size:       4124    3388      21

  Fragments arranged by size: 

                4124    3388      21
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      26    1004    1088    1217    3365    4448    4937    7533 
   Size:         26     978      84     129    2148    1083     489    2596

  Fragments arranged by size: 

                2596    2148    1083     978     489     129      84      26
            
 
MscI TGG'CCA

Cuts at:      0     421    1024    4103    4735    4867    7533 
   Size:        421     603    3079     632     132    2666

  Fragments arranged by size: 

                3079    2666     632     603     421     132
 
MslI CAynn'nnrTG

Cuts at:      0     589     892    1390    3691    5092    6205    6475    6691 
   Size:        589     303     498    2301    1401    1113     270     216

Cuts at:   6691    6763    7129    7533 
   Size:         72     366     404

  Fragments arranged by size: 

                2301    1401    1113     589     498     404     366     303
                 270     216      72
 
MspA1I CmG'CkG

Cuts at:      0     234     530     652     957    1280    1459    1608    1992 
   Size:        234     296     122     305     323     179     149     384

Cuts at:   1992    2132    2727    3309    3585    3647    3801    4268    4634 
   Size:        140     595     582     276      62     154     467     366

Cuts at:   4634    4826    5990    6372    6648    6960    7167    7288    7463 
   Size:        192    1164     382     276     312     207     121     175

Cuts at:   7463    7533 
   Size:         70

  Fragments arranged by size: 

                1164     595     582     467     384     382     366     323
                 312     305     296     276     276     234     207     192
                 179     175     154     149     140     122     121      70
                  62
 
NaeI GCC'GGC

Cuts at:      0      21     747    2394    2534    3741    4213    5448    6145 
   Size:         21     726    1647     140    1207     472    1235     697

Cuts at:   6145    6915    7533 
   Size:        770     618

  Fragments arranged by size: 

                1647    1235    1207     770     726     697     618     472
                 140      21
 
NarI GG'CG_CC

Cuts at:      0    1853    4679    5799    6182    7355    7533 
   Size:       1853    2826    1120     383    1173     178

  Fragments arranged by size: 

                2826    1853    1173    1120     383     178
 
NcoI C'CATG_G

Cuts at:      0    1856    5493    6119    7317    7533 
   Size:       1856    3637     626    1198     216

  Fragments arranged by size: 

                3637    1856    1198     626     216
 
NdeI CA'TA_TG

Cuts at:      0    7102    7533 
   Size:       7102     431
 
NgoMIV G'CCGG_C

Cuts at:      0      19     745    2392    2532    3739    4211    5446    6143 
   Size:         19     726    1647     140    1207     472    1235     697

Cuts at:   6143    6913    7533 
   Size:        770     620

  Fragments arranged by size: 

                1647    1235    1207     770     726     697     620     472
                 140      19
 
NspI r_CATG'y

Cuts at:      0      27    1252    1359    3705    4591    5194    6605    7533 
   Size:         27    1225     107    2346     886     603    1411     928

  Fragments arranged by size: 

                2346    1411    1225     928     886     603     107      27
            
 
PasI CC'CwG_GG

Cuts at:      0    1743    2706    3879    3918    4765    7533 
   Size:       1743     963    1173      39     847    2768

  Fragments arranged by size: 

                2768    1743    1173     963     847      39
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1341    1743    2517    2622    3837    4634    6261    6495 
   Size:       1341     402     774     105    1215     797    1627     234

Cuts at:   6495    6777    6852    7230    7533 
   Size:        282      75     378     303

  Fragments arranged by size: 

                1627    1341    1215     797     774     402     378     303
                 282     234     105      75
 
PfoI T'CCnGG_A

Cuts at:      0      28     803    2105    2234    2810    3023    3338    3653 
   Size:         28     775    1302     129     576     213     315     315

Cuts at:   3653    4448    5297    5453    5752    5963    7533 
   Size:        795     849     156     299     211    1570

  Fragments arranged by size: 

                1570    1302     849     795     775     576     315     315
                 299     213     211     156     129      28
 
PmlI CAC'GTG

Cuts at:      0    2253    7533 
   Size:       2253    5280
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    6643    6675    7533 
   Size:       6643      32     858

  Fragments arranged by size: 

                6643     858      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     165    1338    1957    3513    3633    3969    4617    5379 
   Size:        165    1173     619    1556     120     336     648     762

Cuts at:   5379    6375    7368    7533 
   Size:        996     993     165

  Fragments arranged by size: 

                1556    1173     996     993     762     648     619     336
                 165     165     120
 
PspOMI G'GGCC_C

Cuts at:      0     454    1058    3497    5498    5852    5887    6681    6897 
   Size:        454     604    2439    2001     354      35     794     216

Cuts at:   6897    7533 
   Size:        636

  Fragments arranged by size: 

                2439    2001     794     636     604     454     354     216
                  35
 
PstI C_TGCA'G

Cuts at:      0     506    1124    2087    2303    3104    3209    3404    3446 
   Size:        506     618     963     216     801     105     195      42

Cuts at:   3446    3559    3584    3800    3863    4481    5261    6563    6815 
   Size:        113      25     216      63     618     780    1302     252

Cuts at:   6815    6965    7533 
   Size:        150     568

  Fragments arranged by size: 

                1302     963     801     780     618     618     568     506
                 252     216     216     195     150     113     105      63
                  42      25
 
PvuI CG_AT'CG

Cuts at:      0    6236    7533 
   Size:       6236    1297
 
PvuII CAG'CTG

Cuts at:      0     234    1608    1992    2727    3585    3801    6648    6960 
   Size:        234    1374     384     735     858     216    2847     312

Cuts at:   6960    7533 
   Size:        573

  Fragments arranged by size: 

                2847    1374     858     735     573     384     312     234
                 216
 
RsrII CG'GwC_CG

Cuts at:      0     149    2027    7533 
   Size:        149    1878    5506

  Fragments arranged by size: 

                5506    1878     149
 
SacI G_AGCT'C

Cuts at:      0    2093    6509    6718    7533 
   Size:       2093    4416     209     815

  Fragments arranged by size: 

                4416    2093     815     209
 
SacII CC_GC'GG

Cuts at:      0    3310    7533 
   Size:       3310    4223
 
SanDI GG'GwC_CC

Cuts at:      0    3513    3969    7533 
   Size:       3513     456    3564

  Fragments arranged by size: 

                3564    3513     456
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2908    4204    7533 
   Size:       2908    1296    3329

  Fragments arranged by size: 

                3329    2908    1296
 
SbfI CC_TGCA'GG

Cuts at:      0    3404    3559    7533 
   Size:       3404     155    3974

  Fragments arranged by size: 

                3974    3404     155
 
SexAI A'CCwGG_T

Cuts at:      0    2213    2621    2852    4499    5225    6377    6512    7533 
   Size:       2213     408     231    1647     726    1152     135    1021

  Fragments arranged by size: 

                2213    1647    1152    1021     726     408     231     135
            
 
SfcI C'TryA_G

Cuts at:      0     387     502    1120    2083    2299    3100    3205    3400 
   Size:        387     115     618     963     216     801     105     195

Cuts at:   3400    3442    3555    3580    3796    3859    4477    4953    5257 
   Size:         42     113      25     216      63     618     476     304

Cuts at:   5257    6351    6559    6811    6961    7533 
   Size:       1094     208     252     150     572

  Fragments arranged by size: 

                1094     963     801     618     618     572     476     387
                 304     252     216     216     208     195     150     115
                 113     105      63      42      25
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     844    6161    6937    7533 
   Size:        844    5317     776     596

  Fragments arranged by size: 

                5317     844     776     596
 
SfoI GGC'GCC

Cuts at:      0    1854    4680    5800    6183    7356    7533 
   Size:       1854    2826    1120     383    1173     177

  Fragments arranged by size: 

                2826    1854    1173    1120     383     177
 
SgrAI Cr'CCGG_yG

Cuts at:      0    5789    7533 
   Size:       5789    1744
 
SmaI CCC'GGG

Cuts at:      0    1907    3511    4463    4612    5001    5170    5857    7533 
   Size:       1907    1604     952     149     389     169     687    1676

  Fragments arranged by size: 

                1907    1676    1604     952     687     389     169     149
            
 
SmlI C'TyrA_G

Cuts at:      0     600    2092    3670    5476    5922    7533 
   Size:        600    1492    1578    1806     446    1611

  Fragments arranged by size: 

                1806    1611    1578    1492     600     446
 
SnaBI TAC'GTA

Cuts at:      0    7029    7533 
   Size:       7029     504
 
SphI G_CATG'C

Cuts at:      0    1359    6605    7533 
   Size:       1359    5246     928

  Fragments arranged by size: 

                5246    1359     928
 
StuI AGG'CCT

Cuts at:      0     640    1685    2818    3676    3773    6155    6164    7533 
   Size:        640    1045    1133     858      97    2382       9    1369

  Fragments arranged by size: 

                2382    1369    1133    1045     858     640      97       9
            
 
StyI C'CwwG_G

Cuts at:      0     890    1025    1856    1934    2015    3449    4856    5240 
   Size:        890     135     831      78      81    1434    1407     384

Cuts at:   5240    5493    5532    6119    6614    7304    7317    7533 
   Size:        253      39     587     495     690      13     216

  Fragments arranged by size: 

                1434    1407     890     831     690     587     495     384
                 253     216     135      81      78      39      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     312    1408    3066    3196    4260    5668    6758    7003 
   Size:        312    1096    1658     130    1064    1408    1090     245

Cuts at:   7003    7057    7317    7533 
   Size:         54     260     216

  Fragments arranged by size: 

                1658    1408    1096    1090    1064     312     260     245
                 216     130      54
 
TatI w'GTAC_w

Cuts at:      0    2198    3197    5984    7295    7533 
   Size:       2198     999    2787    1311     238

  Fragments arranged by size: 

                2787    2198    1311     999     238
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1989    2021    2230    2262    3361    3393    3742    3774 
   Size:       1989      32     209      32    1099      32     349      32

Cuts at:   3774    4487    4519    4877    4909    7533 
   Size:        713      32     358      32    2624

  Fragments arranged by size: 

                2624    1989    1099     713     358     349     209      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      94    1645    1942    2790    5227    7533 
   Size:         94    1551     297     848    2437    2306

  Fragments arranged by size: 

                2437    2306    1551     848     297      94
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     465     708     913     981    1863    2501    4353    4920 
   Size:        465     243     205      68     882     638    1852     567

Cuts at:   4920    6117    6192    7029    7533 
   Size:       1197      75     837     504

  Fragments arranged by size: 

                1852    1197     882     837     638     567     504     465
                 243     205      75      68
 
XmaI C'CCGG_G

Cuts at:      0    1905    3509    4461    4610    4999    5168    5855    7533 
   Size:       1905    1604     952     149     389     169     687    1678

  Fragments arranged by size: 

                1905    1678    1604     952     687     389     169     149
            
 
XmnI GAAnn'nnTTC

Cuts at:      0    1585    4180    7533 
   Size:       1585    2595    3353

  Fragments arranged by size: 

                3353    2595    1585
 
ZraI GAC'GTC

Cuts at:      0    5970    7533 
   Size:       5970    1563
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI     FseI    HaeII    Hin4I   HincII     KasI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NspI     PasI    PflMI     PfoI     PmlI     PpiI
    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI    SacII
    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SfoI    SgrAI
     SmaI     SmlI    SnaBI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AhdI     AscI     AseI    AsiSI
    AvrII     BaeI    BfrBI    BglII     BmtI    BsiWI     BsmI    BstBI
  BstZ17I     ClaI     DraI    EcoRI     FspI    FspAI  HindIII     HpaI
     KpnI     MfeI     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI    PshAI     PsiI    PspXI     PsrI     SalI     ScaI     SgfI
     SpeI     SrfI     SspI     SwaI     XbaI     XhoI
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