Restriction map for pF1KA2034
##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

Acc65I      AclI        AflII       Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AvrII       BamHI       BclI        BfrI        BlnI        
BsaBI       BsiWI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BsrGI       Bst1107I    BstBI       
BstZ17I     ClaI        DraI        Eco47III    EcoRV       
EcoT22I     FbaI        HindIII     HpaI        KpnI        
MamI        MluI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
ScaI        SfuI        SgrAI       SnaBI       SpeI        
SspBI       SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AatII        4738  GACGTC               GACGT^C
AccI         1831  GTCGAC               GT^[AC][GT]AC
AccIII       2123  TCCGGA               T^CCGGA
AgeI         1004  ACCGGT               A^CCGGT
Asp700I      1485  GAACTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        5705  TGCGCA               TGC^GCA
BcgI         1778  CGAGGCACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2569  GGATAC               GTATCC(6/5)
BseAI        2123  TCCGGA               T^CCGGA
BspEI        2123  TCCGGA               T^CCGGA
BssHII       2838  GCGCGC               G^CGCGC
BstXI        4472  CCATGGACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI         5270  CGGACCG              CG^G[AT]CCG
EcoRI        3153  GAATTC               G^AATTC
FspI         5705  TGCGCA               TGC^GCA
HincII       1831  GTCGAC               GT[CT]^[GA]AC
HindII       1831  GTCGAC               GT[CT]^[GA]AC
MfeI         2857  CAATTG               C^AATTG
MroI         2123  TCCGGA               T^CCGGA
MseI         5726  TTAA                 T^TAA
MunI         2857  CAATTG               C^AATTG
NspI          551  GCATGC               [GA]CATG^[CT]
PaeR7I        985  CTCGAG               C^TCGAG
PinAI        1004  ACCGGT               A^CCGGT
RsrII        5270  CGGACCG              CG^G[AT]CCG
SalI         1831  GTCGAC               G^TCGAC
SexAI        2094  ACCAGGT              A^CC[AT]GGT
SfiI         3855  GGCCCTGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI          551  GCATGC               GCATG^C
Tru9I        5726  TTAA                 T^TAA
XhoI          985  CTCGAG               C^TCGAG
XmnI         1485  GAACTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AflIII        404  ACACGT               A^C[GA][CT]GT
              518  ACGTGT              
AhdI         3550  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3906  GACTGCACGTC         
AocI         2619  CCTGAGG              CC^T[ACGT]AGG
             6079  CCTGAGG             
AspEI        3550  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3906  GACTGCACGTC         
AspI           12  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1882  GACAACGTC           
BstEII       2529  GGTCACC              G^GT[ACGT]ACC
             5332  GGTAACC             
BstPI        2529  GGTCACC              G^GT[ACGT]ACC
             5332  GGTAACC             
Bsu36I       2619  CCTGAGG              CC^T[ACGT]AGG
             6079  CCTGAGG             
DraIII        502  CACGAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2093  CACCAGGTG           
Eam1105I     3550  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3906  GACTGCACGTC         
Eco81I       2619  CCTGAGG              CC^T[ACGT]AGG
             6079  CCTGAGG             
EcoO65I      2529  GGTCACC              G^GT[ACGT]ACC
             5332  GGTAACC             
FseI         1652  GGCCGGCC             GGCCGG^CC
             5865  GGCCGGCC            
NcoI         4193  CCATGG               C^CATGG
             4472  CCATGG              
PflFI          12  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1882  GACAACGTC           
SapI         5401  GAAGAGC              GCTCTTC(1/4)
             1536  GCTCTTC             
SbfI         4911  CCTGCAGG             CCTGCA^GG
             5463  CCTGCAGG            
Sse8387I     4911  CCTGCAGG             CCTGCA^GG
             5463  CCTGCAGG            
Tth111I        12  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1882  GACAACGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  148 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcnI        BfaI        BglI        BglII       
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrDI       BsrFI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DrdI        DsaI        EaeI        EagI        
EarI        EclXI       Eco52I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       PflMI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        SacI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        SmlI        StuI        
StyI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI        
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