Restriction map for pF1KA2034
 (Circular) (Six-base) MAPSORT of: pF1KA2034.seq  Check: 5704  from: 1  to: 9221



pF1KA2034  9221 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    237    1901    2808     237 
   Size:       1664     907    6650

  Fragments arranged by size: 

                6650    1664     907
 
AatII G_ACGT'C

Cuts at:   4850    4850 
   Size:       9221
 
AccI GT'mk_AC

Cuts at:   1940    6259    1940 
   Size:       4319    4902
 
Acc65I G'GTAC_C

Cuts at:   6237    6237 
   Size:       9221
 
AclI AA'CG_TT

Cuts at:   9061    9061 
   Size:       9221
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    832    1896    2653    3469    3838    5188    5947    7046    7478 
   Size:       1064     757     816     369    1350     759    1099     432

Cuts at:   7478    7831    8493     832 
   Size:        353     662    1560

  Fragments arranged by size: 

                1560    1350    1099    1064     816     759     757     662
                 432     369     353
 
AflIII A'CryG_T

Cuts at:    512     626    6573    8373    8654     512 
   Size:        114    5947    1800     281    1079

  Fragments arranged by size: 

                5947    1800    1079     281     114
 
AgeI A'CCGG_T

Cuts at:   1112    1112 
   Size:       9221
 
AhdI GACnn_n'nnGTC

Cuts at:   3663    4019    3663 
   Size:        356    8865
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2139    4876    2139 
   Size:       2737    6484
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1792    1824    5645    5677    1792 
   Size:         32    3821      32    5336

  Fragments arranged by size: 

                5336    3821      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   1053    2113    2541    2862    3039    3186    3438    3609    3870 
   Size:       1060     428     321     177     147     252     171     261

Cuts at:   3870    3894    4248    4503    4566    4650    5649    6526    7964 
   Size:         24     354     255      63      84     999     877    1438

Cuts at:   7964    1053 
   Size:       2310

  Fragments arranged by size: 

                2310    1438    1060     999     877     428     354     321
                 261     255     252     177     171     147      84      63
                  24
 
ApaI G_GGCC'C

Cuts at:    163     209    2856    3531    3597    3603    4737    4806    5706 
   Size:         46    2647     675      66       6    1134      69     900

Cuts at:   5706     163 
   Size:       3678

  Fragments arranged by size: 

                3678    2647    1134     900     675      69      66      46
                   6
 
ApaLI G'TGCA_C

Cuts at:   8059    8059 
   Size:       9221
 
ApoI r'AATT_y

Cuts at:    922    3226    3261    6225    8574    8830     922 
   Size:       2304      35    2964    2349     256    1313

  Fragments arranged by size: 

                2964    2349    2304    1313     256      35
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       9221
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       9221
 
AvaI C'yCGr_G

Cuts at:    133     169     231    1093    1367    2672    3596    4289    4475 
   Size:         36      62     862     274    1305     924     693     186

Cuts at:   4475    4730    5282    5963    6241     133 
   Size:        255     552     681     278    3113

  Fragments arranged by size: 

                3113    1305     924     862     693     681     552     278
                 274     255     186      62      36
 
BamHI G'GATC_C

Cuts at:   6246    6246 
   Size:       9221
 
BanI G'GyrC_C

Cuts at:    129     144     235     279     615     964    1540    1889    4043 
   Size:         15      91      44     336     349     576     349    2154

Cuts at:   4043    4415    4463    5489    6237    6651    6899    6934     129 
   Size:        372      48    1026     748     414     248      35    2416

  Fragments arranged by size: 

                2416    2154    1026     748     576     414     372     349
                 349     336     248      91      48      44      35      15
            
 
BanII G_rGCy'C

Cuts at:    163     209    1100    2856    2915    3475    3531    3597    3603 
   Size:         46     891    1756      59     560      56      66       6

Cuts at:   3603    3785    4137    4737    4806    5706    5970    6235    7265 
   Size:        182     352     600      69     900     264     265    1030

Cuts at:   7265     163 
   Size:       2119

  Fragments arranged by size: 

                2119    1756    1030     900     891     600     560     352
                 265     264     182      69      66      59      56      46
                   6
 
BbeI G_GCGC'C

Cuts at:    148     968    1544    4047    4419    4467    5493    6655    6903 
   Size:        820     576    2503     372      48    1026    1162     248

Cuts at:   6903     148 
   Size:       2466

  Fragments arranged by size: 

                2503    2466    1162    1026     820     576     372     248
                  48
 
BbsI GAAGACnn'nnnn_

Cuts at:    724    3260    3587    3829    3958    6102     724 
   Size:       2536     327     242     129    2144    3843

  Fragments arranged by size: 

                3843    2536    2144     327     242     129
 
BbvCI CC'TCA_GC

Cuts at:   3164    3329    3871    4310    5342    5759    3164 
   Size:        165     542     439    1032     417    6626

  Fragments arranged by size: 

                6626    1032     542     439     417     165
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1875    1909    1875 
   Size:         34    9187
 
BciVI GTATCCnnnnn_n'

Cuts at:   2671    7119    8175    9221    2671 
   Size:       4448    1056    1046    2671

  Fragments arranged by size: 

                4448    2671    1056    1046
 
BclI T'GATC_A

Cuts at:   6741    6741 
   Size:       9221
 
BglI GCCn_nnn'nGGC

Cuts at:    153     204     760    2956    3970    4306    4480    4810    5330 
   Size:         51     556    2196    1014     336     174     330     520

Cuts at:   5330     153 
   Size:       4044

  Fragments arranged by size: 

                4044    2196    1014     556     520     336     330     174
                  51
 
BglII A'GATC_T

Cuts at:   1580    2039    2582    5291    6736    8773    1580 
   Size:        459     543    2709    1445    2037    2028

  Fragments arranged by size: 

                2709    2037    2028    1445     543     459
 
BlpI GC'TnA_GC

Cuts at:   3271    4210    4814    4831    5606    6333    3271 
   Size:        939     604      17     775     727    6159

  Fragments arranged by size: 

                6159     939     775     727     604      17
 
Bme1580I G_kGCm'C

Cuts at:    134     163     170     209     238    2856    3531    3597    3603 
   Size:         29       7      39      29    2618     675      66       6

Cuts at:   3603    4104    4440    4737    4755    4806    5706    5712    6846 
   Size:        501     336     297      18      51     900       6    1134

Cuts at:   6846    6939    8063     134 
   Size:         93    1124    1292

  Fragments arranged by size: 

                2618    1292    1134    1124     900     675     501     336
                 297      93      66      51      39      29      29      18
                   7       6       6
 
BmgBI CAC'GTC

Cuts at:   4021    4021 
   Size:       9221
 
BmrI ACTGGGnnnn_n'

Cuts at:    157    1357    2294    6433    6514    6849    8993     157 
   Size:       1200     937    4139      81     335    2144     385

  Fragments arranged by size: 

                4139    2144    1200     937     385     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1090    1119    1393    5313    7588    1090 
   Size:         29     274    3920    2275    2723

  Fragments arranged by size: 

                3920    2723    2275     274      29
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    719     728     853    1009    1085    1198    1234    1471    1690 
   Size:          9     125     156      76     113      36     237     219

Cuts at:   1690    2371    2416    2899    3061    3082    3214    3460    3682 
   Size:        681      45     483     162      21     132     246     222

Cuts at:   3682    3745    3766    3850    3937    3988    4072    4168    4357 
   Size:         63      21      84      87      51      84      96     189

Cuts at:   4357    4462    4555    4609    4639    4777    4807    4840    4954 
   Size:        105      93      54      30     138      30      33     114

Cuts at:   4954    4996    5167    5179    5356    5485    5503    5536    5833 
   Size:         42     171      12     177     129      18      33     297

Cuts at:   5833    5917    5959     719 
   Size:         84      42    3981

  Fragments arranged by size: 

                3981     681     483     297     246     237     222     219
                 189     177     171     162     156     138     132     129
                 125     114     113     105      96      93      87      84
                  84      84      76      63      54      51      45      42
                  42      36      33      33      30      30      21      21
                  18      12       9
 
Bpu10I CC'TnA_GC

Cuts at:   2263    3164    3329    3871    4310    5342    5446    5759    5969 
   Size:        901     165     542     439    1032     104     313     210

Cuts at:   5969    2263 
   Size:       5515

  Fragments arranged by size: 

                5515    1032     901     542     439     313     210     165
                 104
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2746    3178    3614    4981    5311    5758    6397    7276    7990 
   Size:        432     436    1367     330     447     639     879     714

Cuts at:   7990    8288    2746 
   Size:        298    3679

  Fragments arranged by size: 

                3679    1367     879     714     639     447     436     432
                 330     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    970    1242    2621    4255    4513    5773    8925     970 
   Size:        272    1379    1634     258    1260    3152    1266

  Fragments arranged by size: 

                3152    1634    1379    1266    1260     272     258
 
BsaAI yAC'GTr

Cuts at:    515     627     759    4777    6574     515 
   Size:        112     132    4018    1797    3162

  Fragments arranged by size: 

                4018    3162    1797     132     112
 
BsaBI GATnn'nnATC

Cuts at:      6    6735    8772       6 
   Size:       6729    2037     455

  Fragments arranged by size: 

                6729    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    145     267     965    1541    4044    4416    4464    4574    4847 
   Size:        122     698     576    2503     372      48     110     273

Cuts at:   4847    5490    5679    6652    6900    7602    9098     145 
   Size:        643     189     973     248     702    1496     268

  Fragments arranged by size: 

                2503    1496     973     702     698     643     576     372
                 273     268     248     189     122     110      48
 
BsaWI w'CCGG_w

Cuts at:   1112    2231    3785    6410    6931    7609    8020    8167    8441 
   Size:       1119    1554    2625     521     678     411     147     274

Cuts at:   8441    1112 
   Size:       1892

  Fragments arranged by size: 

                2625    1892    1554    1119     678     521     411     274
                 147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    502     532     892     922    1792    1822    3073    3103    3772 
   Size:         30     360      30     870      30    1251      30     669

Cuts at:   3772    3802    4141    4171     502 
   Size:         30     339      30    5552

  Fragments arranged by size: 

                5552    1251     870     669     360     339      30      30
                  30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    346     349     897    1078    1081    1693    1813    2635    2956 
   Size:          3     548     181       3     612     120     822     321

Cuts at:   2956    3016    3094    3097    3100    3181    3346    3349    3352 
   Size:         60      78       3       3      81     165       3       3

Cuts at:   3352    3433    3532    3562    3565    3733    3847    3850    4273 
   Size:         81      99      30       3     168     114       3     423

Cuts at:   4273    4354    4357    4438    4441    4468    4672    4774    4777 
   Size:         81       3      81       3      27     204     102       3

Cuts at:   4777    4795    5116    5176    5218    5281    5302    5482    5533 
   Size:         18     321      60      42      63      21     180      51

Cuts at:   5533    5536    5704    5776    5872    5875    5956    5965    5968 
   Size:          3     168      72      96       3      81       9       3

Cuts at:   5968    6136    6157     346 
   Size:        168      21    3410

  Fragments arranged by size: 

                3410     822     612     548     423     321     321     204
                 181     180     168     168     168     165     120     114
                 102      99      96      81      81      81      81      81
                  78      72      63      60      60      51      42      30
                  27      21      21      18       9       3       3       3
                   3       3       3       3       3       3       3       3
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:    179     208     712    1053    2596    2609    3530    3534    3602 
   Size:         29     504     341    1543      13     921       4      68

Cuts at:   3602    4226    4394    4496    5399    5698    6646    7404    8069 
   Size:        624     168     102     903     299     948     758     665

Cuts at:   8069    8477     179 
   Size:        408     923

  Fragments arranged by size: 

                1543     948     923     921     903     758     665     624
                 504     408     341     299     168     102      68      29
                  13       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    457     678    1047    1304    1432    2145    2893    3011    3111 
   Size:        221     369     257     128     713     748     118     100

Cuts at:   3111    3883    4001    4093    4216    5008     457 
   Size:        772     118      92     123     792    4670

  Fragments arranged by size: 

                4670     792     772     748     713     369     257     221
                 128     123     118     118     100      92
 
BsiEI CG_ry'CG

Cuts at:    104    1762    1885    4955    5979    6023    6763    6809    8039 
   Size:       1658     123    3070    1024      44     740      46    1230

Cuts at:   8039     104 
   Size:       1286

  Fragments arranged by size: 

                3070    1658    1286    1230    1024     740     123      46
                  44
 
BsiHKAI G_wGCw'C

Cuts at:   2020    2915    3475    3785    4615    4920    5388    5849    6235 
   Size:        895     560     310     830     305     468     461     386

Cuts at:   6235    7013    7203    8063    2020 
   Size:        778     190     860    3178

  Fragments arranged by size: 

                3178     895     860     830     778     560     468     461
                 386     310     305     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    274     773    1165    2658    2998     274 
   Size:        499     392    1493     340    6497

  Fragments arranged by size: 

                6497    1493     499     392     340
 
Bsp1286I G_dGCh'C

Cuts at:    134     163     170     209     238    1100    2020    2856    2915 
   Size:         29       7      39      29     862     920     836      59

Cuts at:   2915    3475    3531    3597    3603    3785    4104    4137    4440 
   Size:        560      56      66       6     182     319      33     303

Cuts at:   4440    4615    4737    4755    4806    4920    5388    5706    5712 
   Size:        175     122      18      51     114     468     318       6

Cuts at:   5712    5849    5970    6235    6846    6939    7013    7203    7265 
   Size:        137     121     265     611      93      74     190      62

Cuts at:   7265    8063     134 
   Size:        798    1292

  Fragments arranged by size: 

                1292     920     862     836     798     611     560     468
                 319     318     303     265     190     182     175     137
                 122     121     114      93      74      66      62      59
                  56      51      39      33      29      29      18       7
                   6       6
 
BspEI T'CCGG_A

Cuts at:   2231    2231 
   Size:       9221
 
BspHI T'CATG_A

Cuts at:   7653    7653 
   Size:       9221
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    237     457     992    1739    1901    2808    3248    5027    5565 
   Size:        220     535     747     162     907     440    1779     538

Cuts at:   5565    6271    6485    6787    7168     237 
   Size:        706     214     302     381    2290

  Fragments arranged by size: 

                2290    1779     907     747     706     538     535     440
                 381     302     220     214     162
 
BsrBI CCG'CTC

Cuts at:     50     362     489     837    1155    1758    3282    4108    4539 
   Size:        312     127     348     318     603    1524     826     431

Cuts at:   4539    4698    4791    5094    5106    5636    5865    7513    7567 
   Size:        159      93     303      12     530     229    1648      54

Cuts at:   7567    9051    9216      50 
   Size:       1484     165      55

  Fragments arranged by size: 

                1648    1524    1484     826     603     530     431     348
                 318     312     303     229     165     159     127      93
                  55      54      12
 
BsrDI GCAATG_nn'

Cuts at:    885    1262    5726    7133     885 
   Size:        377    4464    1407    2973

  Fragments arranged by size: 

                4464    2973    1407     377
 
BsrFI r'CCGG_y

Cuts at:   1112    1761    2024    4550    5188    5974    6059    7219    7400 
   Size:        649     263    2526     638     786      85    1160     181

Cuts at:   7400    1112 
   Size:       2933

  Fragments arranged by size: 

                2933    2526    1160     786     649     638     263     181
                  85
 
BssHII G'CGCG_C

Cuts at:   2946    2946 
   Size:       9221
 
BssSI C'ACGA_G

Cuts at:    271     610    1207    2329    3850    4103    7492    8200     271 
   Size:        339     597    1122    1521     253    3389     708    1292

  Fragments arranged by size: 

                3389    1521    1292    1122     708     597     339     253
            
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2871    3186    4071    6598    2871 
   Size:        315     885    2527    5494

  Fragments arranged by size: 

                5494    2527     885     315
 
BstBI TT'CG_AA

Cuts at:   7582    7582 
   Size:       9221
 
BstEII G'GTnAC_C

Cuts at:   2637    5440    2637 
   Size:       2803    6418
 
BstXI CCAn_nnnn'nTGG

Cuts at:   4587    4587 
   Size:       9221
 
BstYI r'GATC_y

Cuts at:      1     392    1580    2039    2110    2468    2582    5291    5660 
   Size:        391    1188     459      71     358     114    2709     369

Cuts at:   5660    6246    6713    6736    7071    7317    7623    7635    7721 
   Size:        586     467      23     335     246     306      12      86

Cuts at:   7721    8773       1 
   Size:       1052     449

  Fragments arranged by size: 

                2709    1188    1052     586     467     459     449     391
                 369     358     335     306     246     114      86      71
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:   2728    6188    2728 
   Size:       3460    5761
 
BtgI C'CryG_G

Cuts at:    107     201     382    2356    3882    4301    4523    4580    5126 
   Size:         94     181    1974    1526     419     222      57     546

Cuts at:   5126     107 
   Size:       4202

  Fragments arranged by size: 

                4202    1974    1526     546     419     222     181      94
                  57
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1551    7171    7352    7515    1551 
   Size:       5620     181     163    3257

  Fragments arranged by size: 

                5620    3257     181     163
 
BtsI GCAGTG_nn'

Cuts at:    641    2785    2809    3866    4688    4825    6464     641 
   Size:       2144      24    1057     822     137    1639    3398

  Fragments arranged by size: 

                3398    2144    1639    1057     822     137      24
 
DraI TTT'AAA

Cuts at:   6220    6220 
   Size:       9221
 
DraIII CAC_nnn'GTG

Cuts at:    615    2206     615 
   Size:       1591    7630
 
DrdI GACnn_nn'nnGTC

Cuts at:   1179    2635    2650    6927    8271    1179 
   Size:       1456      15    4277    1344    2129

  Fragments arranged by size: 

                4277    2129    1456    1344      15
 
EaeI y'GGCC_r

Cuts at:    437    1146    1398    1460    1759    1763    1863    1882    2208 
   Size:        709     252      62     299       4     100      19     326

Cuts at:   2208    2276    3305    3569    3641    3677    3885    4952    5063 
   Size:         68    1029     264      72      36     208    1067     111

Cuts at:   5063    5123    5190    5369    5432    5976    6020    6806    6980 
   Size:         60      67     179      63     544      44     786     174

Cuts at:   6980    7371    7398    8743     437 
   Size:        391      27    1345     915

  Fragments arranged by size: 

                1345    1067    1029     915     786     709     544     391
                 326     299     264     252     208     179     174     111
                 100      72      68      67      63      62      60      44
                  36      27      19       4
 
EagI C'GGCC_G

Cuts at:   1759    1882    4952    5976    6020    6806    1759 
   Size:        123    3070    1024      44     786    4174

  Fragments arranged by size: 

                4174    3070    1024     786     123      44
 
EarI CTCTTCn'nnn_

Cuts at:   1168    1267    1489    1651    2329    2446    2612    2933    3112 
   Size:         99     222     162     678     117     166     321     179

Cuts at:   3112    3227    5039    5504    7244    7454    1168 
   Size:        115    1812     465    1740     210    2935

  Fragments arranged by size: 

                2935    1812    1740     678     465     321     222     210
                 179     166     162     117     115      99
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    153     355     370     997    2132    3706    3922    5067    7429 
   Size:        202      15     627    1135    1574     216    1145    2362

Cuts at:   7429    8171    8317    9031     153 
   Size:        742     146     714     343

  Fragments arranged by size: 

                2362    1574    1145    1135     742     714     627     343
                 216     202     146      15
 
EcoICRI GAG'CTC

Cuts at:   2913    3473    3783    6233    2913 
   Size:        560     310    2450    5901

  Fragments arranged by size: 

                5901    2450     560     310
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    719     728     832     853    1009    1085    1198    1234    1471 
   Size:          9     104      21     156      76     113      36     237

Cuts at:   1471    1690    1896    2371    2416    2653    2899    3061    3082 
   Size:        219     206     475      45     237     246     162      21

Cuts at:   3082    3214    3460    3469    3682    3745    3766    3838    3850 
   Size:        132     246       9     213      63      21      72      12

Cuts at:   3850    3937    3988    4072    4168    4357    4462    4555    4609 
   Size:         87      51      84      96     189     105      93      54

Cuts at:   4609    4639    4777    4807    4840    4954    4996    5167    5179 
   Size:         30     138      30      33     114      42     171      12

Cuts at:   5179    5188    5356    5485    5503    5536    5833    5917    5947 
   Size:          9     168     129      18      33     297      84      30

Cuts at:   5947    5959    7046    7478    7831    8493     719 
   Size:         12    1087     432     353     662    1447

  Fragments arranged by size: 

                1447    1087     662     475     432     353     297     246
                 246     237     237     219     213     206     189     171
                 168     162     156     138     132     129     114     113
                 105     104      96      93      87      84      84      76
                  72      63      54      51      45      42      36      33
                  33      30      30      30      21      21      21      18
                  12      12      12       9       9       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:    457    1462    2443    3412    3622    3643    3715    5449    5686 
   Size:       1005     981     969     210      21      72    1734     237

Cuts at:   5686     457 
   Size:       3992

  Fragments arranged by size: 

                3992    1734    1005     981     969     237     210      72
                  21
 
EcoO109I rG'GnC_Cy

Cuts at:    647    1191    1455    2391    2601    2693    3237    3593    3657 
   Size:        544     264     936     210      92     544     356      64

Cuts at:   3657    3708    3891    4286    4488    4734    4803    5331    5702 
   Size:         51     183     395     202     246      69     528     371

Cuts at:   5702    6194    6360     647 
   Size:        492     166    3508

  Fragments arranged by size: 

                3508     936     544     544     528     492     395     371
                 356     264     246     210     202     183     166      92
                  69      64      51
 
EcoRI G'AATT_C

Cuts at:   3261    6225    3261 
   Size:       2964    6257
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2604    7240    2604 
   Size:       4636    4585
 
FseI GG_CCGG'CC

Cuts at:   1765    5978    1765 
   Size:       4213    5008
 
FspI TGC'GCA

Cuts at:   5815    7002    5815 
   Size:       1187    8034
 
HaeII r_GCGC'y

Cuts at:    148     316     609     968    1064    1482    1544    2542    2709 
   Size:        168     293     359      96     418      62     998     167

Cuts at:   2709    2726    2897    3705    3733    3882    4047    4168    4419 
   Size:         17     171     808      28     149     165     121     251

Cuts at:   4419    4467    4503    5325    5493    6655    6903    8133    8904 
   Size:         48      36     822     168    1162     248    1230     771

Cuts at:   8904     148 
   Size:        465

  Fragments arranged by size: 

                1230    1162     998     822     808     771     465     418
                 359     293     251     248     171     168     168     167
                 165     149     121      96      62      48      36      28
                  17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    667     699     892     924    2453    2485    3301    3333    3649 
   Size:         32     193      32    1529      32     816      32     316

Cuts at:   3649    3681    3772    3804    4005    4037    4141    4165    4173 
   Size:         32      91      32     201      32     104      24       8

Cuts at:   4173    4197    4512    4544    5777    5809    5944    5976    7063 
   Size:         24     315      32    1233      32     135      32    1087

Cuts at:   7063    7093    7095    7125     667 
   Size:         30       2      30    2763

  Fragments arranged by size: 

                2763    1529    1233    1087     816     316     315     201
                 193     135     104      91      32      32      32      32
                  32      32      32      32      32      32      30      30
                  24      24       8       2
 
HincII GTy'rAC

Cuts at:   1941    6260    1941 
   Size:       4319    4902
 
KasI G'GCGC_C

Cuts at:    144     964    1540    4043    4415    4463    5489    6651    6899 
   Size:        820     576    2503     372      48    1026    1162     248

Cuts at:   6899     144 
   Size:       2466

  Fragments arranged by size: 

                2503    2466    1162    1026     820     576     372     248
                  48
 
KpnI G_GTAC'C

Cuts at:   6241    6241 
   Size:       9221
 
MfeI C'AATT_G

Cuts at:   2965    2965 
   Size:       9221
 
MluI A'CGCG_T

Cuts at:   8654    8654 
   Size:       9221
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   5483    6138    6161    7980    8164    5483 
   Size:        655      23    1819     184    6540

  Fragments arranged by size: 

                6540    1819     655     184      23
 
MscI TGG'CCA

Cuts at:   1462    2278    3571    3679    5065    5434    6982    1462 
   Size:        816    1293     108    1386     369    1548    3701

  Fragments arranged by size: 

                3701    1548    1386    1293     816     369     108
 
MslI CAynn'nnrTG

Cuts at:    127    1222    1663    5012    7210    7337     127 
   Size:       1095     441    3349    2198     127    2011

  Fragments arranged by size: 

                3349    2198    2011    1095     441     127
 
MspA1I CmG'CkG

Cuts at:    203     384     843    1050    1318    1490    1590    1881    2312 
   Size:        181     459     207     268     172     100     291     431

Cuts at:   2312    2358    2613    2668    2742    2934    3192    3328    3510 
   Size:         46     255      55      74     192     258     136     182

Cuts at:   3510    3534    3606    3915    4038    4062    4230    4335    4351 
   Size:         24      72     309     123      24     168     105      16

Cuts at:   4351    4599    4669    4951    5061    5073    5481    5605    5649 
   Size:        248      70     282     110      12     408     124      44

Cuts at:   5649    5895    5925    5937    6332    6646    7006    7790    8035 
   Size:        246      30      12     395     314     360     784     245

Cuts at:   8035     203 
   Size:       1389

  Fragments arranged by size: 

                1389     784     459     431     408     395     360     314
                 309     291     282     268     258     255     248     246
                 245     207     192     182     181     172     168     136
                 124     123     110     105     100      74      72      70
                  55      46      44      30      24      24      16      12
                  12
 
NaeI GCC'GGC

Cuts at:   1763    4552    5190    5976    7402    1763 
   Size:       2789     638     786    1426    3582

  Fragments arranged by size: 

                3582    2789    1426     786     638
 
NarI GG'CG_CC

Cuts at:    145     965    1541    4044    4416    4464    5490    6652    6900 
   Size:        820     576    2503     372      48    1026    1162     248

Cuts at:   6900     145 
   Size:       2466

  Fragments arranged by size: 

                2503    2466    1162    1026     820     576     372     248
                  48
 
NcoI C'CATG_G

Cuts at:    107    4301    4580     107 
   Size:       4194     279    4748

  Fragments arranged by size: 

                4748    4194     279
 
NgoMIV G'CCGG_C

Cuts at:   1761    4550    5188    5974    7400    1761 
   Size:       2789     638     786    1426    3582

  Fragments arranged by size: 

                3582    2789    1426     786     638
 
NspI r_CATG'y

Cuts at:    663    6274    6774    8377     663 
   Size:       5611     500    1603    1507

  Fragments arranged by size: 

                5611    1603    1507     500
 
PasI CC'CwG_GG

Cuts at:   3894    3894 
   Size:       9221
 
PciI A'CATG_T

Cuts at:   8373    8373 
   Size:       9221
 
PflMI CCAn_nnn'nTGG

Cuts at:   1053    1958    2206    2541    3438    3894    4503    4650    1053 
   Size:        905     248     335     897     456     609     147    5624

  Fragments arranged by size: 

                5624     905     897     609     456     335     248     147
            
 
PfoI T'CCnGG_A

Cuts at:    482    1166    1559    2393    2444    3248    5663    5885     482 
   Size:        684     393     834      51     804    2415     222    3818

  Fragments arranged by size: 

                3818    2415     834     804     684     393     222      51
            
 
PmeI GTTT'AAAC

Cuts at:   6220    6220 
   Size:       9221
 
PmlI CAC'GTG

Cuts at:    627     759    4777     627 
   Size:        132    4018    5071

  Fragments arranged by size: 

                5071    4018     132
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1792    1824    7667    7699    1792 
   Size:         32    5843      32    3314

  Fragments arranged by size: 

                5843    3314      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   1191    2391    2601    3237    3657    3708    4286    6194    1191 
   Size:       1200     210     636     420      51     578    1908    4218

  Fragments arranged by size: 

                4218    1908    1200     636     578     420     210      51
            
 
PspOMI G'GGCC_C

Cuts at:    159     205    2852    3527    3593    3599    4733    4802    5702 
   Size:         46    2647     675      66       6    1134      69     900

Cuts at:   5702     159 
   Size:       3678

  Fragments arranged by size: 

                3678    2647    1134     900     675      69      66      46
                   6
 
PspXI vC'TCGA_Gb

Cuts at:   1093    1093 
   Size:       9221
 
PstI C_TGCA'G

Cuts at:    668    1640    1736    2118    2819    2846    2867    3191    3485 
   Size:        972      96     382     701      27      21     324     294

Cuts at:   3485    3560    4013    4067    4130    4256    4382    4559    5024 
   Size:         75     453      54      63     126     126     177     465

Cuts at:   5024    5360    5576    5993    6268    6953     668 
   Size:        336     216     417     275     685    2936

  Fragments arranged by size: 

                2936     972     701     685     465     453     417     382
                 336     324     294     275     216     177     126     126
                  96      75      63      54      27      21
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       9221
 
PvuII CAG'CTG

Cuts at:    843    1050    1318    1590    2613    2668    2934    3192    3328 
   Size:        207     268     272    1023      55     266     258     136

Cuts at:   3328    3534    3606    3915    4062    4230    4335    4351    4669 
   Size:        206      72     309     147     168     105      16     318

Cuts at:   4669    5061    5073    5481    5605    5649    5895    5925    5937 
   Size:        392      12     408     124      44     246      30      12

Cuts at:   5937    6646    7006     843 
   Size:        709     360    3058

  Fragments arranged by size: 

                3058    1023     709     408     392     360     318     309
                 272     268     266     258     246     207     206     168
                 147     136     124     105      72      55      44      30
                  16      12      12
 
RsrII CG'GwC_CG

Cuts at:   5379    7416    5379 
   Size:       2037    7184
 
SacI G_AGCT'C

Cuts at:   2915    3475    3785    6235    2915 
   Size:        560     310    2450    5901

  Fragments arranged by size: 

                5901    2450     560     310
 
SacII CC_GC'GG

Cuts at:    204     385    2359     204 
   Size:        181    1974    7066

  Fragments arranged by size: 

                7066    1974     181
 
SalI G'TCGA_C

Cuts at:   1939    6258    1939 
   Size:       4319    4902
 
SanDI GG'GwC_CC

Cuts at:   4286    6194    4286 
   Size:       1908    7313
 
SapI GCTCTTCn'nnn_

Cuts at:   1651    5504    7244    7454    1651 
   Size:       3853    1740     210    3418

  Fragments arranged by size: 

                3853    3418    1740     210
 
SbfI CC_TGCA'GG

Cuts at:   5024    5576    6268    5024 
   Size:        552     692    7977

  Fragments arranged by size: 

                7977     692     552
 
ScaI AGT'ACT

Cuts at:   8738    8738 
   Size:       9221
 
SexAI A'CCwGG_T

Cuts at:   2202    2202 
   Size:       9221
 
SfcI C'TryA_G

Cuts at:     33     664    1636    1732    2114    2815    2842    2863    2881 
   Size:        631     972      96     382     701      27      21      18

Cuts at:   2881    3124    3187    3481    3556    4009    4063    4126    4252 
   Size:        243      63     294      75     453      54      63     126

Cuts at:   4252    4378    4555    5020    5356    5572    5989    6264    6949 
   Size:        126     177     465     336     216     417     275     685

Cuts at:   6949    7917    8108      33 
   Size:        968     191    1146

  Fragments arranged by size: 

                1146     972     968     701     685     631     465     453
                 417     382     336     294     275     243     216     191
                 177     126     126      96      75      63      63      54
                  27      21      18
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   3970    3970 
   Size:       9221
 
SfoI GGC'GCC

Cuts at:    146     966    1542    4045    4417    4465    5491    6653    6901 
   Size:        820     576    2503     372      48    1026    1162     248

Cuts at:   6901     146 
   Size:       2466

  Fragments arranged by size: 

                2503    2466    1162    1026     820     576     372     248
                  48
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       9221
 
SmaI CCC'GGG

Cuts at:    135    2674    3598    4291    4477    5284    5965    6243     135 
   Size:       2539     924     693     186     807     681     278    3113

  Fragments arranged by size: 

                3113    2539     924     807     693     681     278     186
            
 
SmlI C'TyrA_G

Cuts at:   1093    2761    3157    3629    4996    5326    5737    6376    7291 
   Size:       1668     396     472    1367     330     411     639     915

Cuts at:   7291    8005    8267    1093 
   Size:        714     262    2047

  Fragments arranged by size: 

                2047    1668    1367     915     714     639     472     411
                 396     330     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       9221
 
SphI G_CATG'C

Cuts at:    663    6274    6774     663 
   Size:       5611     500    3110

  Fragments arranged by size: 

                5611    3110     500
 
SrfI GCCC'GGGC

Cuts at:    135    3598     135 
   Size:       3463    5758
 
StuI AGG'CCT

Cuts at:    463     871    2518     463 
   Size:        408    1647    7166

  Fragments arranged by size: 

                7166    1647     408
 
StyI C'CwwG_G

Cuts at:    107    1016    1463    1517    1536    2082    2492    2798    3686 
   Size:        909     447      54      19     546     410     306     888

Cuts at:   3686    4280    4301    4580    5624    6355    6709     107 
   Size:        594      21     279    1044     731     354    2619

  Fragments arranged by size: 

                2619    1044     909     888     731     594     546     447
                 410     354     306     279      54      21      19
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    267     456     655    2493    5674    6804    6937    7396    7605 
   Size:        189     199    1838    3181    1130     133     459     209

Cuts at:   7605     267 
   Size:       1883

  Fragments arranged by size: 

                3181    1883    1838    1130     459     209     199     189
                 133
 
TatI w'GTAC_w

Cuts at:   4671    8736    4671 
   Size:       4065    5156
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1337    1369    1786    1818    3073    3105    5795    5827    8738 
   Size:         32     417      32    1255      32    2690      32    2911

Cuts at:   8738    8770    1337 
   Size:         32    1788

  Fragments arranged by size: 

                2911    2690    1788    1255     417      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:    123    1993    7018     123 
   Size:       1870    5025    2326

  Fragments arranged by size: 

                5025    2326    1870
 
XbaI T'CTAG_A

Cuts at:   6252    6252 
   Size:       9221
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1230    1665    2499    4272    4584    4971    6716    1230 
   Size:        435     834    1773     312     387    1745    3735

  Fragments arranged by size: 

                3735    1773    1745     834     435     387     312
 
XhoI C'TCGA_G

Cuts at:   1093    1093 
   Size:       9221
 
XmaI C'CCGG_G

Cuts at:    133    2672    3596    4289    4475    5282    5963    6241     133 
   Size:       2539     924     693     186     807     681     278    3113

  Fragments arranged by size: 

                3113    2539     924     807     693     681     278     186
            
 
XmnI GAAnn'nnTTC

Cuts at:   1597    1597 
   Size:       9221
 
ZraI GAC'GTC

Cuts at:   4848    4848 
   Size:       9221
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FseI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     SrfI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AleI     AscI    AvrII     BaeI    BfrBI     BmtI
    BsiWI     BsmI    BsrGI  BstZ17I     ClaI    CspCI    EcoRV    FspAI
  HindIII     HpaI     NdeI     NheI     NotI     NruI     NsiI     PacI
    PshAI     PsiI     PsrI    SgrAI    SnaBI     SspI     SwaI
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