Restriction map for pF1KA2034
 (Linear) (Six-base) MAPSORT of: KIAA2034.seq  Check: 9423  from: 1  to: 6108



KIAA2034.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     129    1793    2700    6108 
   Size:        129    1664     907    3408

  Fragments arranged by size: 

                3408    1664     907     129
 
AatII G_ACGT'C

Cuts at:      0    4742    6108 
   Size:       4742    1366
 
AccI GT'mk_AC

Cuts at:      0    1832    6108 
   Size:       1832    4276
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     724    1788    2545    3361    3730    5080    5839    6108 
   Size:        724    1064     757     816     369    1350     759     269

  Fragments arranged by size: 

                1350    1064     816     759     757     724     369     269
            
 
AflIII A'CryG_T

Cuts at:      0     404     518    6108 
   Size:        404     114    5590

  Fragments arranged by size: 

                5590     404     114
 
AgeI A'CCGG_T

Cuts at:      0    1004    6108 
   Size:       1004    5104
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3555    3911    6108 
   Size:       3555     356    2197

  Fragments arranged by size: 

                3555    2197     356
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2031    4768    6108 
   Size:       2031    2737    1340

  Fragments arranged by size: 

                2737    2031    1340
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1684    1716    5537    5569    6108 
   Size:       1684      32    3821      32     539

  Fragments arranged by size: 

                3821    1684     539      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     945    2005    2433    2754    2931    3078    3330    3501 
   Size:        945    1060     428     321     177     147     252     171

Cuts at:   3501    3762    3786    4140    4395    4458    4542    5541    6108 
   Size:        261      24     354     255      63      84     999     567

  Fragments arranged by size: 

                1060     999     945     567     428     354     321     261
                 255     252     177     171     147      84      63      24
            
 
ApaI G_GGCC'C

Cuts at:      0      55     101    2748    3423    3489    3495    4629    4698 
   Size:         55      46    2647     675      66       6    1134      69

Cuts at:   4698    5598    6108 
   Size:        900     510

  Fragments arranged by size: 

                2647    1134     900     675     510      69      66      55
                  46       6
 
ApoI r'AATT_y

Cuts at:      0     814    3118    3153    6108 
   Size:        814    2304      35    2955

  Fragments arranged by size: 

                2955    2304     814      35
 
AvaI C'yCGr_G

Cuts at:      0      25      61     123     985    1259    2564    3488    4181 
   Size:         25      36      62     862     274    1305     924     693

Cuts at:   4181    4367    4622    5174    5855    6108 
   Size:        186     255     552     681     253

  Fragments arranged by size: 

                1305     924     862     693     681     552     274     255
                 253     186      62      36      25
 
BanI G'GyrC_C

Cuts at:      0      21      36     127     171     507     856    1432    1781 
   Size:         21      15      91      44     336     349     576     349

Cuts at:   1781    3935    4307    4355    5381    6108 
   Size:       2154     372      48    1026     727

  Fragments arranged by size: 

                2154    1026     727     576     372     349     349     336
                  91      48      44      21      15
 
BanII G_rGCy'C

Cuts at:      0      55     101     992    2748    2807    3367    3423    3489 
   Size:         55      46     891    1756      59     560      56      66

Cuts at:   3489    3495    3677    4029    4629    4698    5598    5862    6108 
   Size:          6     182     352     600      69     900     264     246

  Fragments arranged by size: 

                1756     900     891     600     560     352     264     246
                 182      69      66      59      56      55      46       6
            
 
BbeI G_GCGC'C

Cuts at:      0      40     860    1436    3939    4311    4359    5385    6108 
   Size:         40     820     576    2503     372      48    1026     723

  Fragments arranged by size: 

                2503    1026     820     723     576     372      48      40
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     616    3152    3479    3721    3850    5994    6108 
   Size:        616    2536     327     242     129    2144     114

  Fragments arranged by size: 

                2536    2144     616     327     242     129     114
 
BbvCI CC'TCA_GC

Cuts at:      0    3056    3221    3763    4202    5234    5651    6108 
   Size:       3056     165     542     439    1032     417     457

  Fragments arranged by size: 

                3056    1032     542     457     439     417     165
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1767    1801    6108 
   Size:       1767      34    4307

  Fragments arranged by size: 

                4307    1767      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2563    6108 
   Size:       2563    3545
 
BglI GCCn_nnn'nGGC

Cuts at:      0      45      96     652    2848    3862    4198    4372    4702 
   Size:         45      51     556    2196    1014     336     174     330

Cuts at:   4702    5222    6108 
   Size:        520     886

  Fragments arranged by size: 

                2196    1014     886     556     520     336     330     174
                  51      45
 
BglII A'GATC_T

Cuts at:      0    1472    1931    2474    5183    6108 
   Size:       1472     459     543    2709     925

  Fragments arranged by size: 

                2709    1472     925     543     459
 
BlpI GC'TnA_GC

Cuts at:      0    3163    4102    4706    4723    5498    6108 
   Size:       3163     939     604      17     775     610

  Fragments arranged by size: 

                3163     939     775     610     604      17
 
Bme1580I G_kGCm'C

Cuts at:      0      26      55      62     101     130    2748    3423    3489 
   Size:         26      29       7      39      29    2618     675      66

Cuts at:   3489    3495    3996    4332    4629    4647    4698    5598    5604 
   Size:          6     501     336     297      18      51     900       6

Cuts at:   5604    6108 
   Size:        504

  Fragments arranged by size: 

                2618     900     675     504     501     336     297      66
                  51      39      29      29      26      18       7       6
                   6
 
BmgBI CAC'GTC

Cuts at:      0    3913    6108 
   Size:       3913    2195
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      49    1249    2186    6108 
   Size:         49    1200     937    3922

  Fragments arranged by size: 

                3922    1200     937      49
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     982    1011    1285    5205    6108 
   Size:        982      29     274    3920     903

  Fragments arranged by size: 

                3920     982     903     274      29
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     611     620     745     901     977    1090    1126    1363 
   Size:        611       9     125     156      76     113      36     237

Cuts at:   1363    1582    2263    2308    2791    2953    2974    3106    3352 
   Size:        219     681      45     483     162      21     132     246

Cuts at:   3352    3574    3637    3658    3742    3829    3880    3964    4060 
   Size:        222      63      21      84      87      51      84      96

Cuts at:   4060    4249    4354    4447    4501    4531    4669    4699    4732 
   Size:        189     105      93      54      30     138      30      33

Cuts at:   4732    4846    4888    5059    5071    5248    5377    5395    5428 
   Size:        114      42     171      12     177     129      18      33

Cuts at:   5428    5725    5809    5851    6108 
   Size:        297      84      42     257

  Fragments arranged by size: 

                 681     611     483     297     257     246     237     222
                 219     189     177     171     162     156     138     132
                 129     125     114     113     105      96      93      87
                  84      84      84      76      63      54      51      45
                  42      42      36      33      33      30      30      21
                  21      18      12       9
 
Bpu10I CC'TnA_GC

Cuts at:      0    2155    3056    3221    3763    4202    5234    5338    5651 
   Size:       2155     901     165     542     439    1032     104     313

Cuts at:   5651    5861    6108 
   Size:        210     247

  Fragments arranged by size: 

                2155    1032     901     542     439     313     247     210
                 165     104
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2638    3070    3506    4873    5203    5650    6108 
   Size:       2638     432     436    1367     330     447     458

  Fragments arranged by size: 

                2638    1367     458     447     436     432     330
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     862    1134    2513    4147    4405    5665    6108 
   Size:        862     272    1379    1634     258    1260     443

  Fragments arranged by size: 

                1634    1379    1260     862     443     272     258
 
BsaAI yAC'GTr

Cuts at:      0     407     519     651    4669    6108 
   Size:        407     112     132    4018    1439

  Fragments arranged by size: 

                4018    1439     407     132     112
 
BsaHI Gr'CG_yC

Cuts at:      0      37     159     857    1433    3936    4308    4356    4466 
   Size:         37     122     698     576    2503     372      48     110

Cuts at:   4466    4739    5382    5571    6108 
   Size:        273     643     189     537

  Fragments arranged by size: 

                2503     698     643     576     537     372     273     189
                 122     110      48      37
 
BsaWI w'CCGG_w

Cuts at:      0    1004    2123    3677    6108 
   Size:       1004    1119    1554    2431

  Fragments arranged by size: 

                2431    1554    1119    1004
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     394     424     784     814    1684    1714    2965    2995 
   Size:        394      30     360      30     870      30    1251      30

Cuts at:   2995    3664    3694    4033    4063    6108 
   Size:        669      30     339      30    2045

  Fragments arranged by size: 

                2045    1251     870     669     394     360     339      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     238     241     789     970     973    1585    1705    2527 
   Size:        238       3     548     181       3     612     120     822

Cuts at:   2527    2848    2908    2986    2989    2992    3073    3238    3241 
   Size:        321      60      78       3       3      81     165       3

Cuts at:   3241    3244    3325    3424    3454    3457    3625    3739    3742 
   Size:          3      81      99      30       3     168     114       3

Cuts at:   3742    4165    4246    4249    4330    4333    4360    4564    4666 
   Size:        423      81       3      81       3      27     204     102

Cuts at:   4666    4669    4687    5008    5068    5110    5173    5194    5374 
   Size:          3      18     321      60      42      63      21     180

Cuts at:   5374    5425    5428    5596    5668    5764    5767    5848    5857 
   Size:         51       3     168      72      96       3      81       9

Cuts at:   5857    5860    6028    6049    6108 
   Size:          3     168      21      59

  Fragments arranged by size: 

                 822     612     548     423     321     321     238     204
                 181     180     168     168     168     165     120     114
                 102      99      96      81      81      81      81      81
                  78      72      63      60      60      59      51      42
                  30      27      21      21      18       9       3       3
                   3       3       3       3       3       3       3       3
                   3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0      71     100     604     945    2488    2501    3422    3426 
   Size:         71      29     504     341    1543      13     921       4

Cuts at:   3426    3494    4118    4286    4388    5291    5590    6108 
   Size:         68     624     168     102     903     299     518

  Fragments arranged by size: 

                1543     921     903     624     518     504     341     299
                 168     102      71      68      29      13       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     570     939    1196    1324    2037    2785    2903 
   Size:        349     221     369     257     128     713     748     118

Cuts at:   2903    3003    3775    3893    3985    4108    4900    6108 
   Size:        100     772     118      92     123     792    1208

  Fragments arranged by size: 

                1208     792     772     748     713     369     349     257
                 221     128     123     118     118     100      92
 
BsiEI CG_ry'CG

Cuts at:      0    1654    1777    4847    5871    5915    6108 
   Size:       1654     123    3070    1024      44     193

  Fragments arranged by size: 

                3070    1654    1024     193     123      44
 
BsiHKAI G_wGCw'C

Cuts at:      0    1912    2807    3367    3677    4507    4812    5280    5741 
   Size:       1912     895     560     310     830     305     468     461

Cuts at:   5741    6108 
   Size:        367

  Fragments arranged by size: 

                1912     895     830     560     468     461     367     310
                 305
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     166     665    1057    2550    2890    6108 
   Size:        166     499     392    1493     340    3218

  Fragments arranged by size: 

                3218    1493     499     392     340     166
 
Bsp1286I G_dGCh'C

Cuts at:      0      26      55      62     101     130     992    1912    2748 
   Size:         26      29       7      39      29     862     920     836

Cuts at:   2748    2807    3367    3423    3489    3495    3677    3996    4029 
   Size:         59     560      56      66       6     182     319      33

Cuts at:   4029    4332    4507    4629    4647    4698    4812    5280    5598 
   Size:        303     175     122      18      51     114     468     318

Cuts at:   5598    5604    5741    5862    6108 
   Size:          6     137     121     246

  Fragments arranged by size: 

                 920     862     836     560     468     319     318     303
                 246     182     175     137     122     121     114      66
                  59      56      51      39      33      29      29      26
                  18       7       6       6
 
BspEI T'CCGG_A

Cuts at:      0    2123    6108 
   Size:       2123    3985
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     129     349     884    1631    1793    2700    3140    4919 
   Size:        129     220     535     747     162     907     440    1779

Cuts at:   4919    5457    6108 
   Size:        538     651

  Fragments arranged by size: 

                1779     907     747     651     538     535     440     220
                 162     129
 
BsrBI CCG'CTC

Cuts at:      0     254     381     729    1047    1650    3174    4000    4431 
   Size:        254     127     348     318     603    1524     826     431

Cuts at:   4431    4590    4683    4986    4998    5528    5757    6108 
   Size:        159      93     303      12     530     229     351

  Fragments arranged by size: 

                1524     826     603     530     431     351     348     318
                 303     254     229     159     127      93      12
 
BsrDI GCAATG_nn'

Cuts at:      0     777    1154    5618    6108 
   Size:        777     377    4464     490

  Fragments arranged by size: 

                4464     777     490     377
 
BsrFI r'CCGG_y

Cuts at:      0    1004    1653    1916    4442    5080    5866    5951    6108 
   Size:       1004     649     263    2526     638     786      85     157

  Fragments arranged by size: 

                2526    1004     786     649     638     263     157      85
            
 
BssHII G'CGCG_C

Cuts at:      0    2838    6108 
   Size:       2838    3270
 
BssSI C'ACGA_G

Cuts at:      0     163     502    1099    2221    3742    3995    6108 
   Size:        163     339     597    1122    1521     253    2113

  Fragments arranged by size: 

                2113    1521    1122     597     339     253     163
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2763    3078    3963    6108 
   Size:       2763     315     885    2145

  Fragments arranged by size: 

                2763    2145     885     315
 
BstEII G'GTnAC_C

Cuts at:      0    2529    5332    6108 
   Size:       2529    2803     776

  Fragments arranged by size: 

                2803    2529     776
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    4479    6108 
   Size:       4479    1629
 
BstYI r'GATC_y

Cuts at:      0     284    1472    1931    2002    2360    2474    5183    5552 
   Size:        284    1188     459      71     358     114    2709     369

Cuts at:   5552    6108 
   Size:        556

  Fragments arranged by size: 

                2709    1188     556     459     369     358     284     114
                  71
 
Bsu36I CC'TnA_GG

Cuts at:      0    2620    6080    6108 
   Size:       2620    3460      28

  Fragments arranged by size: 

                3460    2620      28
 
BtgI C'CryG_G

Cuts at:      0      93     274    2248    3774    4193    4415    4472    5018 
   Size:         93     181    1974    1526     419     222      57     546

Cuts at:   5018    6108 
   Size:       1090

  Fragments arranged by size: 

                1974    1526    1090     546     419     222     181      93
                  57
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1443    6108 
   Size:       1443    4665
 
BtsI GCAGTG_nn'

Cuts at:      0     533    2677    2701    3758    4580    4717    6108 
   Size:        533    2144      24    1057     822     137    1391

  Fragments arranged by size: 

                2144    1391    1057     822     533     137      24
 
DraIII CAC_nnn'GTG

Cuts at:      0     507    2098    6108 
   Size:        507    1591    4010

  Fragments arranged by size: 

                4010    1591     507
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1071    2527    2542    6108 
   Size:       1071    1456      15    3566

  Fragments arranged by size: 

                3566    1456    1071      15
 
EaeI y'GGCC_r

Cuts at:      0     329    1038    1290    1352    1651    1655    1755    1774 
   Size:        329     709     252      62     299       4     100      19

Cuts at:   1774    2100    2168    3197    3461    3533    3569    3777    4844 
   Size:        326      68    1029     264      72      36     208    1067

Cuts at:   4844    4955    5015    5082    5261    5324    5868    5912    6108 
   Size:        111      60      67     179      63     544      44     196

  Fragments arranged by size: 

                1067    1029     709     544     329     326     299     264
                 252     208     196     179     111     100      72      68
                  67      63      62      60      44      36      19       4
            
 
EagI C'GGCC_G

Cuts at:      0    1651    1774    4844    5868    5912    6108 
   Size:       1651     123    3070    1024      44     196

  Fragments arranged by size: 

                3070    1651    1024     196     123      44
 
EarI CTCTTCn'nnn_

Cuts at:      0    1060    1159    1381    1543    2221    2338    2504    2825 
   Size:       1060      99     222     162     678     117     166     321

Cuts at:   2825    3004    3119    4931    5396    6108 
   Size:        179     115    1812     465     712

  Fragments arranged by size: 

                1812    1060     712     678     465     321     222     179
                 166     162     117     115      99
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      45     247     262     889    2024    3598    3814    4959 
   Size:         45     202      15     627    1135    1574     216    1145

Cuts at:   4959    6108 
   Size:       1149

  Fragments arranged by size: 

                1574    1149    1145    1135     627     216     202      45
                  15
 
EcoICRI GAG'CTC

Cuts at:      0    2805    3365    3675    6108 
   Size:       2805     560     310    2433

  Fragments arranged by size: 

                2805    2433     560     310
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     611     620     724     745     901     977    1090    1126 
   Size:        611       9     104      21     156      76     113      36

Cuts at:   1126    1363    1582    1788    2263    2308    2545    2791    2953 
   Size:        237     219     206     475      45     237     246     162

Cuts at:   2953    2974    3106    3352    3361    3574    3637    3658    3730 
   Size:         21     132     246       9     213      63      21      72

Cuts at:   3730    3742    3829    3880    3964    4060    4249    4354    4447 
   Size:         12      87      51      84      96     189     105      93

Cuts at:   4447    4501    4531    4669    4699    4732    4846    4888    5059 
   Size:         54      30     138      30      33     114      42     171

Cuts at:   5059    5071    5080    5248    5377    5395    5428    5725    5809 
   Size:         12       9     168     129      18      33     297      84

Cuts at:   5809    5839    5851    6108 
   Size:         30      12     257

  Fragments arranged by size: 

                 611     475     297     257     246     246     237     237
                 219     213     206     189     171     168     162     156
                 138     132     129     114     113     105     104      96
                  93      87      84      84      76      72      63      54
                  51      45      42      36      33      33      30      30
                  30      21      21      21      18      12      12      12
                   9       9       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     349    1354    2335    3304    3514    3535    3607    5341 
   Size:        349    1005     981     969     210      21      72    1734

Cuts at:   5341    5578    6108 
   Size:        237     530

  Fragments arranged by size: 

                1734    1005     981     969     530     349     237     210
                  72      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     539    1083    1347    2283    2493    2585    3129    3485 
   Size:        539     544     264     936     210      92     544     356

Cuts at:   3485    3549    3600    3783    4178    4380    4626    4695    5223 
   Size:         64      51     183     395     202     246      69     528

Cuts at:   5223    5594    6086    6108 
   Size:        371     492      22

  Fragments arranged by size: 

                 936     544     544     539     528     492     395     371
                 356     264     246     210     202     183      92      69
                  64      51      22
 
EcoRI G'AATT_C

Cuts at:      0    3153    6108 
   Size:       3153    2955
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2496    6108 
   Size:       2496    3612
 
FseI GG_CCGG'CC

Cuts at:      0    1657    5870    6108 
   Size:       1657    4213     238

  Fragments arranged by size: 

                4213    1657     238
 
FspI TGC'GCA

Cuts at:      0    5707    6108 
   Size:       5707     401
 
HaeII r_GCGC'y

Cuts at:      0      40     208     501     860     956    1374    1436    2434 
   Size:         40     168     293     359      96     418      62     998

Cuts at:   2434    2601    2618    2789    3597    3625    3774    3939    4060 
   Size:        167      17     171     808      28     149     165     121

Cuts at:   4060    4311    4359    4395    5217    5385    6108 
   Size:        251      48      36     822     168     723

  Fragments arranged by size: 

                 998     822     808     723     418     359     293     251
                 171     168     168     167     165     149     121      96
                  62      48      40      36      28      17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     559     591     784     816    2345    2377    3193    3225 
   Size:        559      32     193      32    1529      32     816      32

Cuts at:   3225    3541    3573    3664    3696    3897    3929    4033    4057 
   Size:        316      32      91      32     201      32     104      24

Cuts at:   4057    4065    4089    4404    4436    5669    5701    5836    5868 
   Size:          8      24     315      32    1233      32     135      32

Cuts at:   5868    6108 
   Size:        240

  Fragments arranged by size: 

                1529    1233     816     559     316     315     240     201
                 193     135     104      91      32      32      32      32
                  32      32      32      32      32      32      24      24
                   8
 
HincII GTy'rAC

Cuts at:      0    1833    6108 
   Size:       1833    4275
 
KasI G'GCGC_C

Cuts at:      0      36     856    1432    3935    4307    4355    5381    6108 
   Size:         36     820     576    2503     372      48    1026     727

  Fragments arranged by size: 

                2503    1026     820     727     576     372      48      36
            
 
MfeI C'AATT_G

Cuts at:      0    2857    6108 
   Size:       2857    3251
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    5375    6030    6053    6108 
   Size:       5375     655      23      55

  Fragments arranged by size: 

                5375     655      55      23
 
MscI TGG'CCA

Cuts at:      0    1354    2170    3463    3571    4957    5326    6108 
   Size:       1354     816    1293     108    1386     369     782

  Fragments arranged by size: 

                1386    1354    1293     816     782     369     108
 
MslI CAynn'nnrTG

Cuts at:      0      19    1114    1555    4904    6108 
   Size:         19    1095     441    3349    1204

  Fragments arranged by size: 

                3349    1204    1095     441      19
 
MspA1I CmG'CkG

Cuts at:      0      95     276     735     942    1210    1382    1482    1773 
   Size:         95     181     459     207     268     172     100     291

Cuts at:   1773    2204    2250    2505    2560    2634    2826    3084    3220 
   Size:        431      46     255      55      74     192     258     136

Cuts at:   3220    3402    3426    3498    3807    3930    3954    4122    4227 
   Size:        182      24      72     309     123      24     168     105

Cuts at:   4227    4243    4491    4561    4843    4953    4965    5373    5497 
   Size:         16     248      70     282     110      12     408     124

Cuts at:   5497    5541    5787    5817    5829    6108 
   Size:         44     246      30      12     279

  Fragments arranged by size: 

                 459     431     408     309     291     282     279     268
                 258     255     248     246     207     192     182     181
                 172     168     136     124     123     110     105     100
                  95      74      72      70      55      46      44      30
                  24      24      16      12      12
 
NaeI GCC'GGC

Cuts at:      0    1655    4444    5082    5868    6108 
   Size:       1655    2789     638     786     240

  Fragments arranged by size: 

                2789    1655     786     638     240
 
NarI GG'CG_CC

Cuts at:      0      37     857    1433    3936    4308    4356    5382    6108 
   Size:         37     820     576    2503     372      48    1026     726

  Fragments arranged by size: 

                2503    1026     820     726     576     372      48      37
            
 
NcoI C'CATG_G

Cuts at:      0    4193    4472    6108 
   Size:       4193     279    1636

  Fragments arranged by size: 

                4193    1636     279
 
NgoMIV G'CCGG_C

Cuts at:      0    1653    4442    5080    5866    6108 
   Size:       1653    2789     638     786     242

  Fragments arranged by size: 

                2789    1653     786     638     242
 
NspI r_CATG'y

Cuts at:      0     555    6108 
   Size:        555    5553
 
PasI CC'CwG_GG

Cuts at:      0    3786    6108 
   Size:       3786    2322
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     945    1850    2098    2433    3330    3786    4395    4542 
   Size:        945     905     248     335     897     456     609     147

Cuts at:   4542    6108 
   Size:       1566

  Fragments arranged by size: 

                1566     945     905     897     609     456     335     248
                 147
 
PfoI T'CCnGG_A

Cuts at:      0     374    1058    1451    2285    2336    3140    5555    5777 
   Size:        374     684     393     834      51     804    2415     222

Cuts at:   5777    6108 
   Size:        331

  Fragments arranged by size: 

                2415     834     804     684     393     374     331     222
                  51
 
PmlI CAC'GTG

Cuts at:      0     519     651    4669    6108 
   Size:        519     132    4018    1439

  Fragments arranged by size: 

                4018    1439     519     132
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1684    1716    6108 
   Size:       1684      32    4392

  Fragments arranged by size: 

                4392    1684      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1083    2283    2493    3129    3549    3600    4178    6086 
   Size:       1083    1200     210     636     420      51     578    1908

Cuts at:   6086    6108 
   Size:         22

  Fragments arranged by size: 

                1908    1200    1083     636     578     420     210      51
                  22
 
PspOMI G'GGCC_C

Cuts at:      0      51      97    2744    3419    3485    3491    4625    4694 
   Size:         51      46    2647     675      66       6    1134      69

Cuts at:   4694    5594    6108 
   Size:        900     514

  Fragments arranged by size: 

                2647    1134     900     675     514      69      66      51
                  46       6
 
PspXI vC'TCGA_Gb

Cuts at:      0     985    6108 
   Size:        985    5123
 
PstI C_TGCA'G

Cuts at:      0     560    1532    1628    2010    2711    2738    2759    3083 
   Size:        560     972      96     382     701      27      21     324

Cuts at:   3083    3377    3452    3905    3959    4022    4148    4274    4451 
   Size:        294      75     453      54      63     126     126     177

Cuts at:   4451    4916    5252    5468    5885    6108 
   Size:        465     336     216     417     223

  Fragments arranged by size: 

                 972     701     560     465     453     417     382     336
                 324     294     223     216     177     126     126      96
                  75      63      54      27      21
 
PvuII CAG'CTG

Cuts at:      0     735     942    1210    1482    2505    2560    2826    3084 
   Size:        735     207     268     272    1023      55     266     258

Cuts at:   3084    3220    3426    3498    3807    3954    4122    4227    4243 
   Size:        136     206      72     309     147     168     105      16

Cuts at:   4243    4561    4953    4965    5373    5497    5541    5787    5817 
   Size:        318     392      12     408     124      44     246      30

Cuts at:   5817    5829    6108 
   Size:         12     279

  Fragments arranged by size: 

                1023     735     408     392     318     309     279     272
                 268     266     258     246     207     206     168     147
                 136     124     105      72      55      44      30      16
                  12      12
 
RsrII CG'GwC_CG

Cuts at:      0    5271    6108 
   Size:       5271     837
 
SacI G_AGCT'C

Cuts at:      0    2807    3367    3677    6108 
   Size:       2807     560     310    2431

  Fragments arranged by size: 

                2807    2431     560     310
 
SacII CC_GC'GG

Cuts at:      0      96     277    2251    6108 
   Size:         96     181    1974    3857

  Fragments arranged by size: 

                3857    1974     181      96
 
SalI G'TCGA_C

Cuts at:      0    1831    6108 
   Size:       1831    4277
 
SanDI GG'GwC_CC

Cuts at:      0    4178    6086    6108 
   Size:       4178    1908      22

  Fragments arranged by size: 

                4178    1908      22
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1543    5396    6108 
   Size:       1543    3853     712

  Fragments arranged by size: 

                3853    1543     712
 
SbfI CC_TGCA'GG

Cuts at:      0    4916    5468    6108 
   Size:       4916     552     640

  Fragments arranged by size: 

                4916     640     552
 
SexAI A'CCwGG_T

Cuts at:      0    2094    6108 
   Size:       2094    4014
 
SfcI C'TryA_G

Cuts at:      0     556    1528    1624    2006    2707    2734    2755    2773 
   Size:        556     972      96     382     701      27      21      18

Cuts at:   2773    3016    3079    3373    3448    3901    3955    4018    4144 
   Size:        243      63     294      75     453      54      63     126

Cuts at:   4144    4270    4447    4912    5248    5464    5881    6108 
   Size:        126     177     465     336     216     417     227

  Fragments arranged by size: 

                 972     701     556     465     453     417     382     336
                 294     243     227     216     177     126     126      96
                  75      63      63      54      27      21      18
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    3862    6108 
   Size:       3862    2246
 
SfoI GGC'GCC

Cuts at:      0      38     858    1434    3937    4309    4357    5383    6108 
   Size:         38     820     576    2503     372      48    1026     725

  Fragments arranged by size: 

                2503    1026     820     725     576     372      48      38
            
 
SmaI CCC'GGG

Cuts at:      0      27    2566    3490    4183    4369    5176    5857    6108 
   Size:         27    2539     924     693     186     807     681     251

  Fragments arranged by size: 

                2539     924     807     693     681     251     186      27
            
 
SmlI C'TyrA_G

Cuts at:      0     985    2653    3049    3521    4888    5218    5629    6108 
   Size:        985    1668     396     472    1367     330     411     479

  Fragments arranged by size: 

                1668    1367     985     479     472     411     396     330
            
 
SphI G_CATG'C

Cuts at:      0     555    6108 
   Size:        555    5553
 
SrfI GCCC'GGGC

Cuts at:      0      27    3490    6108 
   Size:         27    3463    2618

  Fragments arranged by size: 

                3463    2618      27
 
StuI AGG'CCT

Cuts at:      0     355     763    2410    6108 
   Size:        355     408    1647    3698

  Fragments arranged by size: 

                3698    1647     408     355
 
StyI C'CwwG_G

Cuts at:      0     908    1355    1409    1428    1974    2384    2690    3578 
   Size:        908     447      54      19     546     410     306     888

Cuts at:   3578    4172    4193    4472    5516    6108 
   Size:        594      21     279    1044     592

  Fragments arranged by size: 

                1044     908     888     594     592     546     447     410
                 306     279      54      21      19
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     159     348     547    2385    5566    6108 
   Size:        159     189     199    1838    3181     542

  Fragments arranged by size: 

                3181    1838     542     199     189     159
 
TatI w'GTAC_w

Cuts at:      0    4563    6108 
   Size:       4563    1545
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1229    1261    1678    1710    2965    2997    5687    5719 
   Size:       1229      32     417      32    1255      32    2690      32

Cuts at:   5719    6108 
   Size:        389

  Fragments arranged by size: 

                2690    1255    1229     417     389      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0      15    1885    6108 
   Size:         15    1870    4223

  Fragments arranged by size: 

                4223    1870      15
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1122    1557    2391    4164    4476    4863    6108 
   Size:       1122     435     834    1773     312     387    1245

  Fragments arranged by size: 

                1773    1245    1122     834     435     387     312
 
XhoI C'TCGA_G

Cuts at:      0     985    6108 
   Size:        985    5123
 
XmaI C'CCGG_G

Cuts at:      0      25    2564    3488    4181    4367    5174    5855    6108 
   Size:         25    2539     924     693     186     807     681     253

  Fragments arranged by size: 

                2539     924     807     693     681     253     186      25
            
 
XmnI GAAnn'nnTTC

Cuts at:      0    1489    6108 
   Size:       1489    4619
 
ZraI GAC'GTC

Cuts at:      0    4740    6108 
   Size:       4740    1368
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AgeI     AhdI     AlfI
     AloI    AlwNI     ApaI     ApoI     AvaI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    HaeII
    Hin4I   HincII     KasI     MfeI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PasI    PflMI     PfoI
     PmlI     PpiI    PpuMI   PspOMI    PspXI     PstI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfcI
     SfiI     SfoI     SmaI     SmlI     SphI     SrfI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AleI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BclI    BfrBI     BmtI    BsaBI
    BsiWI     BsmI    BspHI    BsrGI    BstBI  BstZ17I     ClaI    CspCI
     DraI    EcoRV    FspAI  HindIII     HpaI     KpnI     MluI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI    PshAI
     PsiI     PsrI     PvuI     ScaI     SgfI    SgrAI    SnaBI     SpeI
     SspI     SwaI     XbaI
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