Restriction map for
pF1KA2021
(Circular) (Six-base) MAPSORT of: pF1KA2021.seq Check: 6437 from: 1 to: 5021
pF1KA2021 5021 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 695 2059 695
Size: 1364 3657
Acc65I G'GTAC_C
Cuts at: 2037 2037
Size: 5021
AclI AA'CG_TT
Cuts at: 4861 4861
Size: 5021
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 824 862 922 2846 3278 4025 4293 724
Size: 100 38 60 1924 432 747 268 1452
Fragments arranged by size:
1924 1452 747 432 268 100 60 38
AfeI AGC'GCT
Cuts at: 1219 1219
Size: 5021
AflIII A'CryG_T
Cuts at: 2373 3477 4454 2373
Size: 1104 977 2940
Fragments arranged by size:
2940 1104 977
AgeI A'CCGG_T
Cuts at: 3409 3409
Size: 5021
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 448 1813 1985 448
Size: 1365 172 3484
Fragments arranged by size:
3484 1365 172
AlwNI CAG_nnn'CTG
Cuts at: 444 885 944 1113 1182 1504 1584 1650 1878
Size: 441 59 169 69 322 80 66 228
Cuts at: 1878 2326 3893 444
Size: 448 1567 1572
Fragments arranged by size:
1572 1567 448 441 322 228 169 80
69 66 59
ApaI G_GGCC'C
Cuts at: 1836 1836
Size: 5021
ApaLI G'TGCA_C
Cuts at: 364 3791 364
Size: 3427 1594
ApoI r'AATT_y
Cuts at: 2025 4374 4630 2025
Size: 2349 256 2416
Fragments arranged by size:
2416 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5021
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5021
AvaI C'yCGr_G
Cuts at: 334 2041 334
Size: 1707 3314
BamHI G'GATC_C
Cuts at: 2046 2046
Size: 5021
BanI G'GyrC_C
Cuts at: 143 194 1627 2037 2451 2699 2734 143
Size: 51 1433 410 414 248 35 2430
Fragments arranged by size:
2430 1433 414 410 248 51 35
BanII G_rGCy'C
Cuts at: 283 892 1130 1279 1412 1836 2035 3065 283
Size: 609 238 149 133 424 199 1030 2239
Fragments arranged by size:
2239 1030 609 424 238 199 149 133
BbeI G_GCGC'C
Cuts at: 147 198 2455 2703 147
Size: 51 2257 248 2465
Fragments arranged by size:
2465 2257 248 51
BbsI GAAGACnn'nnnn_
Cuts at: 820 940 2018 820
Size: 120 1078 3823
Fragments arranged by size:
3823 1078 120
BbvCI CC'TCA_GC
Cuts at: 671 671
Size: 5021
BciVI GTATCCnnnnn_n'
Cuts at: 2919 3680 5021 2919
Size: 761 1341 2919
Fragments arranged by size:
2919 1341 761
BclI T'GATC_A
Cuts at: 2541 2541
Size: 5021
BglI GCCn_nnn'nGGC
Cuts at: 1831 1831
Size: 5021
BglII A'GATC_T
Cuts at: 354 932 2536 4573 354
Size: 578 1604 2037 802
Fragments arranged by size:
2037 1604 802 578
BlpI GC'TnA_GC
Cuts at: 1273 1853 2133 1273
Size: 580 280 4161
Fragments arranged by size:
4161 580 280
Bme1580I G_kGCm'C
Cuts at: 368 1836 2646 2739 3795 368
Size: 1468 810 93 1056 1594
Fragments arranged by size:
1594 1468 1056 810 93
BmrI ACTGGGnnnn_n'
Cuts at: 350 981 2233 2314 2649 4793 350
Size: 631 1252 81 335 2144 578
Fragments arranged by size:
2144 1252 631 578 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3388 3388
Size: 5021
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 664 784 921 1123 1561 1833 242
Size: 422 120 137 202 438 272 3430
Fragments arranged by size:
3430 438 422 272 202 137 120
Bpu10I CC'TnA_GC
Cuts at: 277 671 824 886 1244 277
Size: 394 153 62 358 4054
Fragments arranged by size:
4054 394 358 153 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1848 2197 3076 3568 3866 1848
Size: 349 879 492 298 3003
Fragments arranged by size:
3003 879 492 349 298
BsaI GGTCTCn'nnnn_
Cuts at: 631 1571 1880 4725 631
Size: 940 309 2845 927
Fragments arranged by size:
2845 940 927 309
BsaAI yAC'GTr
Cuts at: 2374 2374
Size: 5021
BsaBI GATnn'nnATC
Cuts at: 6 2535 4572 6
Size: 2529 2037 455
Fragments arranged by size:
2529 2037 455
BsaHI Gr'CG_yC
Cuts at: 144 195 2452 2700 3402 4898 144
Size: 51 2257 248 702 1496 267
Fragments arranged by size:
2257 1496 702 267 248 51
BsaWI w'CCGG_w
Cuts at: 234 2210 2731 3409 3683 3830 4241 234
Size: 1976 521 678 274 147 411 1014
Fragments arranged by size:
1976 1014 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 874 904 874
Size: 30 4991
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 607 610 613 616 619 622 625 661 882
Size: 3 3 3 3 3 3 36 221
Cuts at: 882 1168 1282 1501 1504 607
Size: 286 114 219 3 4124
Fragments arranged by size:
4124 286 221 219 114 36 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 153 576 819 2446 3204 3781 4277 153
Size: 423 243 1627 758 577 496 897
Fragments arranged by size:
1627 897 758 577 496 423 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1172 1424 1573 1172
Size: 252 149 4620
Fragments arranged by size:
4620 252 149
BsiEI CG_ry'CG
Cuts at: 104 2563 2609 3817 104
Size: 2459 46 1208 1308
Fragments arranged by size:
2459 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 368 892 1160 1514 1688 2035 2813 3003 3795
Size: 524 268 354 174 347 778 190 792
Cuts at: 3795 368
Size: 1594
Fragments arranged by size:
1594 792 778 524 354 347 268 190
174
BsmI GAATG_Cn'
Cuts at: 1393 1393
Size: 5021
Bsp1286I G_dGCh'C
Cuts at: 283 368 892 1130 1160 1279 1412 1514 1688
Size: 85 524 238 30 119 133 102 174
Cuts at: 1688 1836 2035 2646 2739 2813 3003 3065 3795
Size: 148 199 611 93 74 190 62 730
Cuts at: 3795 283
Size: 1509
Fragments arranged by size:
1509 730 611 524 238 199 190 174
148 133 119 102 93 85 74 62
30
BspEI T'CCGG_A
Cuts at: 234 4241 234
Size: 4007 1014
BspHI T'CATG_A
Cuts at: 4197 4197
Size: 5021
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1098 2071 2285 2587 2968 1098
Size: 973 214 302 381 3151
Fragments arranged by size:
3151 973 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3313 3367 4851 5016 50
Size: 3263 54 1484 165 55
Fragments arranged by size:
3263 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 908 1014 1196 1322 1529 2933 908
Size: 106 182 126 207 1404 2996
Fragments arranged by size:
2996 1404 207 182 126 106
BsrFI r'CCGG_y
Cuts at: 146 252 3019 3200 3409 146
Size: 106 2767 181 209 1758
Fragments arranged by size:
2767 1758 209 181 106
BssSI C'ACGA_G
Cuts at: 3292 3650 3292
Size: 358 4663
BstAPI GCAn_nnn'nTGC
Cuts at: 1113 1504 1587 2398 1113
Size: 391 83 811 3736
Fragments arranged by size:
3736 811 391 83
BstBI TT'CG_AA
Cuts at: 1617 3382 1617
Size: 1765 3256
BstXI CCAn_nnnn'nTGG
Cuts at: 1101 1455 1542 1101
Size: 354 87 4580
Fragments arranged by size:
4580 354 87
BstYI r'GATC_y
Cuts at: 1 354 932 2046 2513 2536 2871 3117 4129
Size: 353 578 1114 467 23 335 246 1012
Cuts at: 4129 4215 4227 4573 1
Size: 86 12 346 449
Fragments arranged by size:
1114 1012 578 467 449 353 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 632 786 1785 107
Size: 525 154 999 3343
Fragments arranged by size:
3343 999 525 154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2971 3152 3315 2971
Size: 181 163 4677
Fragments arranged by size:
4677 181 163
BtsI GCAGTG_nn'
Cuts at: 432 920 1505 2264 432
Size: 488 585 759 3189
Fragments arranged by size:
3189 759 585 488
DraI TTT'AAA
Cuts at: 2020 2020
Size: 5021
DraIII CAC_nnn'GTG
Cuts at: 138 1518 1905 138
Size: 1380 387 3254
Fragments arranged by size:
3254 1380 387
DrdI GACnn_nn'nnGTC
Cuts at: 2727 3585 2727
Size: 858 4163
EaeI y'GGCC_r
Cuts at: 226 1526 1896 1976 2606 2780 3171 3198 4543
Size: 1300 370 80 630 174 391 27 1345
Cuts at: 4543 226
Size: 704
Fragments arranged by size:
1345 1300 704 630 391 370 174 80
27
EagI C'GGCC_G
Cuts at: 2606 2606
Size: 5021
EarI CTCTTCn'nnn_
Cuts at: 3044 3254 3044
Size: 210 4811
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 292 3229 3539 3685 4831 292
Size: 2937 310 146 1146 482
Fragments arranged by size:
2937 1146 482 310 146
EcoICRI GAG'CTC
Cuts at: 890 2033 890
Size: 1143 3878
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 664 724 784 824 862 921 922 1123
Size: 422 60 60 40 38 59 1 201
Cuts at: 1123 1561 1833 2846 3278 4025 4293 242
Size: 438 272 1013 432 747 268 970
Fragments arranged by size:
1013 970 747 438 432 422 272 268
201 60 60 59 40 38 1
EcoNI CCTnn'n_nnAGG
Cuts at: 1105 1150 1105
Size: 45 4976
EcoO109I rG'GnC_Cy
Cuts at: 396 782 882 1110 1337 1355 1371 1832 2160
Size: 386 100 228 227 18 16 461 328
Cuts at: 2160 396
Size: 3257
Fragments arranged by size:
3257 461 386 328 228 227 100 18
16
EcoRI G'AATT_C
Cuts at: 2025 2025
Size: 5021
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1907 3040 1907
Size: 1133 3888
FspI TGC'GCA
Cuts at: 508 2802 508
Size: 2294 2727
HaeII r_GCGC'y
Cuts at: 147 198 246 313 1221 2455 2703 3725 4704
Size: 51 48 67 908 1234 248 1022 979
Cuts at: 4704 147
Size: 464
Fragments arranged by size:
1234 1022 979 908 464 248 67 51
48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 229 261 518 550 807 839 874 906 919
Size: 32 257 32 257 32 35 32 13
Cuts at: 919 951 1237 1269 1399 1431 2863 2893 2895
Size: 32 286 32 130 32 1432 30 2
Cuts at: 2895 2925 229
Size: 30 2325
Fragments arranged by size:
2325 1432 286 257 257 130 35 32
32 32 32 32 32 32 30 30
13 2
HincII GTy'rAC
Cuts at: 2060 2060
Size: 5021
KasI G'GCGC_C
Cuts at: 143 194 2451 2699 143
Size: 51 2257 248 2465
Fragments arranged by size:
2465 2257 248 51
KpnI G_GTAC'C
Cuts at: 2041 2041
Size: 5021
MluI A'CGCG_T
Cuts at: 4454 4454
Size: 5021
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1103 1367 3692 3876 1103
Size: 264 2325 184 2248
Fragments arranged by size:
2325 2248 264 184
MscI TGG'CCA
Cuts at: 1978 2782 1978
Size: 804 4217
MslI CAynn'nnrTG
Cuts at: 913 1453 3010 3137 913
Size: 540 1557 127 2797
Fragments arranged by size:
2797 1557 540 127
MspA1I CmG'CkG
Cuts at: 157 670 841 1173 1672 2132 2446 2806 3819
Size: 513 171 332 499 460 314 360 1013
Cuts at: 3819 4064 157
Size: 245 1114
Fragments arranged by size:
1114 1013 513 499 460 360 332 314
245 171
NaeI GCC'GGC
Cuts at: 254 3202 254
Size: 2948 2073
NarI GG'CG_CC
Cuts at: 144 195 2452 2700 144
Size: 51 2257 248 2465
Fragments arranged by size:
2465 2257 248 51
NcoI C'CATG_G
Cuts at: 107 1785 107
Size: 1678 3343
NgoMIV G'CCGG_C
Cuts at: 252 3200 252
Size: 2948 2073
NspI r_CATG'y
Cuts at: 2074 2574 3481 2074
Size: 500 907 3614
Fragments arranged by size:
3614 907 500
PasI CC'CwG_GG
Cuts at: 1302 1312 1302
Size: 10 5011
PciI A'CATG_T
Cuts at: 3477 3477
Size: 5021
PflMI CCAn_nnn'nTGG
Cuts at: 160 1905 160
Size: 1745 3276
PfoI T'CCnGG_A
Cuts at: 1100 1100
Size: 5021
PmeI GTTT'AAAC
Cuts at: 2020 2020
Size: 5021
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 4160
Size: 32 4989
PpuMI rG'GwC_Cy
Cuts at: 882 1110 1371 882
Size: 228 261 4532
Fragments arranged by size:
4532 261 228
PspOMI G'GGCC_C
Cuts at: 1832 1832
Size: 5021
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1023 1055 1023
Size: 32 4989
PstI C_TGCA'G
Cuts at: 440 591 1178 1509 1745 2068 2753 440
Size: 151 587 331 236 323 685 2708
Fragments arranged by size:
2708 685 587 331 323 236 151
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5021
PvuII CAG'CTG
Cuts at: 157 841 1173 2446 2806 157
Size: 684 332 1273 360 2372
Fragments arranged by size:
2372 1273 684 360 332
RsrII CG'GwC_CG
Cuts at: 3216 3216
Size: 5021
SacI G_AGCT'C
Cuts at: 892 2035 892
Size: 1143 3878
SalI G'TCGA_C
Cuts at: 2058 2058
Size: 5021
SapI GCTCTTCn'nnn_
Cuts at: 3044 3254 3044
Size: 210 4811
SbfI CC_TGCA'GG
Cuts at: 2068 2068
Size: 5021
ScaI AGT'ACT
Cuts at: 1032 4538 1032
Size: 3506 1515
SexAI A'CCwGG_T
Cuts at: 1514 1514
Size: 5021
SfcI C'TryA_G
Cuts at: 33 436 559 587 1174 1375 1505 1741 1776
Size: 403 123 28 587 201 130 236 35
Cuts at: 1776 2064 2749 3742 3933 33
Size: 288 685 993 191 1121
Fragments arranged by size:
1121 993 685 587 403 288 236 201
191 130 123 35 28
SfoI GGC'GCC
Cuts at: 145 196 2453 2701 145
Size: 51 2257 248 2465
Fragments arranged by size:
2465 2257 248 51
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5021
SmaI CCC'GGG
Cuts at: 2043 2043
Size: 5021
SmlI C'TyrA_G
Cuts at: 1827 2176 3091 3583 3845 1827
Size: 349 915 492 262 3003
Fragments arranged by size:
3003 915 492 349 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5021
SphI G_CATG'C
Cuts at: 2074 2574 2074
Size: 500 4521
StyI C'CwwG_G
Cuts at: 107 182 1367 1541 1560 1785 2155 2509 107
Size: 75 1185 174 19 225 370 354 2619
Fragments arranged by size:
2619 1185 370 354 225 174 75 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1325 1615 2604 2737 3196 3405 1325
Size: 290 989 133 459 209 2941
Fragments arranged by size:
2941 989 459 290 209 133
TatI w'GTAC_w
Cuts at: 1030 1469 1611 4536 1030
Size: 439 142 2925 1515
Fragments arranged by size:
2925 1515 439 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 411 443 1748 1780 4538 4570 411
Size: 32 1305 32 2758 32 862
Fragments arranged by size:
2758 1305 862 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2818 2818
Size: 5021
XbaI T'CTAG_A
Cuts at: 2052 2052
Size: 5021
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 639 1669 2516 639
Size: 1030 847 3144
Fragments arranged by size:
3144 1030 847
XmaI C'CCGG_G
Cuts at: 2041 2041
Size: 5021
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI