Restriction map for pF1KA1948
 (Linear) (Six-base) MAPSORT of: KIAA1948.seq  Check: 1436  from: 1  to: 1596



KIAA1948.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     396     603    1275    1596 
   Size:        396     207     672     321

  Fragments arranged by size: 

                 672     396     321     207
 
AflII C'TTAA_G

Cuts at:      0     334    1596 
   Size:        334    1262
 
ApoI r'AATT_y

Cuts at:      0     288     569    1073    1157    1493    1577    1596 
   Size:        288     281     504      84     336      84      19

  Fragments arranged by size: 

                 504     336     288     281      84      84      19
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     399     432    1596 
   Size:        399      33    1164

  Fragments arranged by size: 

                1164     399      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     222    1596 
   Size:        222    1374
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      42     400    1596 
   Size:         42     358    1196

  Fragments arranged by size: 

                1196     358      42
 
BfrBI ATG'CAT

Cuts at:      0     741    1596 
   Size:        741     855
 
BglII A'GATC_T

Cuts at:      0     717    1596 
   Size:        717     879
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     142     291    1596 
   Size:        142     149    1305

  Fragments arranged by size: 

                1305     149     142
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32    1596 
   Size:         32    1564
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383    1596 
   Size:        383    1213
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     132     229     241     346     387    1596 
   Size:        132      97      12     105      41    1209

  Fragments arranged by size: 

                1209     132     105      97      41      12
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     226     256    1046    1076    1596 
   Size:        226      30     790      30     520

  Fragments arranged by size: 

                 790     520     226      30      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     614     734    1596 
   Size:        614     120     862

  Fragments arranged by size: 

                 862     614     120
 
BsmI GAATG_Cn'

Cuts at:      0     937    1030    1198    1282    1409    1596 
   Size:        937      93     168      84     127     187

  Fragments arranged by size: 

                 937     187     168     127      93      84
 
BsrGI T'GTAC_A

Cuts at:      0      89    1596 
   Size:         89    1507
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      56    1596 
   Size:         56    1540
 
BstYI r'GATC_y

Cuts at:      0     717    1596 
   Size:        717     879
 
BtgI C'CryG_G

Cuts at:      0     327    1128    1596 
   Size:        327     801     468

  Fragments arranged by size: 

                 801     468     327
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     924     959    1596 
   Size:        924      35     637

  Fragments arranged by size: 

                 924     637      35
 
DraI TTT'AAA

Cuts at:      0     350    1596 
   Size:        350    1246
 
EarI CTCTTCn'nnn_

Cuts at:      0      47    1596 
   Size:         47    1549
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     396     603    1275    1596 
   Size:         32     364     207     672     321

  Fragments arranged by size: 

                 672     364     321     207      32
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     401    1596 
   Size:        401    1195
 
EcoRI G'AATT_C

Cuts at:      0     569    1073    1157    1493    1596 
   Size:        569     504      84     336     103

  Fragments arranged by size: 

                 569     504     336     103      84
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1557    1596 
   Size:       1557      39
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     349     381     882     914    1596 
   Size:        349      32     501      32     682

  Fragments arranged by size: 

                 682     501     349      32      32
 
HindIII A'AGCT_T

Cuts at:      0     698     904    1596 
   Size:        698     206     692

  Fragments arranged by size: 

                 698     692     206
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      86    1596 
   Size:         86    1510
 
MslI CAynn'nnrTG

Cuts at:      0     135     578     662     746     830     998    1082    1166 
   Size:        135     443      84      84      84     168      84      84

Cuts at:   1166    1250    1334    1502    1596 
   Size:         84      84     168      94

  Fragments arranged by size: 

                 443     168     168     135      94      84      84      84
                  84      84      84      84
 
NcoI C'CATG_G

Cuts at:      0     327    1596 
   Size:        327    1269
 
NdeI CA'TA_TG

Cuts at:      0     706    1596 
   Size:        706     890
 
NsiI A_TGCA'T

Cuts at:      0     743    1596 
   Size:        743     853
 
PsiI TTA'TAA

Cuts at:      0    1349    1596 
   Size:       1349     247
 
SfcI C'TryA_G

Cuts at:      0     114    1596 
   Size:        114    1482
 
SmlI C'TyrA_G

Cuts at:      0     334     398    1596 
   Size:        334      64    1198

  Fragments arranged by size: 

                1198     334      64
 
StuI AGG'CCT

Cuts at:      0     952    1036    1288    1372    1456    1540    1596 
   Size:        952      84     252      84      84      84      56

  Fragments arranged by size: 

                 952     252      84      84      84      84      56
 
StyI C'CwwG_G

Cuts at:      0     190     327    1596 
   Size:        190     137    1269

  Fragments arranged by size: 

                1269     190     137
 
TatI w'GTAC_w

Cuts at:      0      89     726     978    1596 
   Size:         89     637     252     618

  Fragments arranged by size: 

                 637     618     252      89
 
XmnI GAAnn'nnTTC

Cuts at:      0     532    1288    1497    1596 
   Size:        532     756     209      99

  Fragments arranged by size: 

                 756     532     209      99
 

 Enzymes that do cut:

     AcuI    AflII     ApoI     BaeI     BbsI    BciVI    BfrBI    BglII
     BmrI     BpmI    BpuEI     BsaI    BsaXI     BsgI     BsmI    BsrGI
    BstXI    BstYI     BtgI    CspCI     DraI     EarI  Eco57MI    EcoNI
    EcoRI     FalI    Hin4I  HindIII     MmeI     MslI     NcoI     NdeI
     NsiI     PsiI     SfcI     SmlI     StuI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI     BclI     BglI     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI EcoO109I    EcoRV     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI
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