Restriction map for pF1KA1868
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# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AflII       AgeI        AocI        Aor51HI     AscI        
AseI        AsnI        AspI        AvaI        AviII       
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BciVI       BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BseAI       BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BspEI       
BspMI       BsrFI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoT22I     FseI        
FspI        HincII      HindII      HpaI        KspI        
MamI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    SspI        SwaI        
Tth111I     XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       1985  GGTACC               G^GTACC
AcyI         1334  GGCGCC               G[GA]^CG[CT]C
AflIII       1481  ACATGT               A^C[GA][CT]GT
AhdI         1405  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        475  GTGCAC               G^TGCAC
ApaI         1310  GGGCCC               GGGCC^C
ApaLI         475  GTGCAC               G^TGCAC
Asp718I      1985  GGTACC               G^GTACC
AspEI        1405  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI         1334  GGCGCC               GGCGC^C
BclI          779  TGATCA               T^GATCA
BcnI         1966  CCCGG                CC^[GC]GG
BsaHI        1334  GGCGCC               G[GA]^CG[CT]C
BsaWI         345  ACCGGA               [AT]^CCGG[AT]
BsmI          100  GAATGC               GAATGC(1/-1)
Bsp1407I     1577  TGTACA               T^GTACA
BspLU11I     1481  ACATGT               A^CATGT
BsrBI        1932  GAGCGG               CCGCTC(-3/-3)
BsrGI        1577  TGTACA               T^GTACA
BstAPI       1011  GCAGGCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         464  CCAACCCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI          487  TTTAAA               TTT^AAA
DsaI         1862  CCACGG               C^C[GA][CT]GG
Eam1105I     1405  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        1282  GATATC               GAT^ATC
FbaI          779  TGATCA               T^GATCA
Hin1I        1334  GGCGCC               G[GA]^CG[CT]C
KasI         1334  GGCGCC               G^GCGCC
KpnI         1985  GGTACC               GGTAC^C
MslI         1233  CACAAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI         1334  GGCGCC               GG^CGCC
NciI         1966  CCCGG                CC^[GC]GG
NspI         1481  ACATGT               [GA]CATG^[CT]
PpuMI        1397  AGGACCC              [GA]G^G[AT]CC[CT]
PvuII         646  CAGCTG               CAG^CTG
SacI         1571  GAGCTC               GAGCT^C
SapI         1717  GAAGAGC              GCTCTTC(1/4)
SfoI         1334  GGCGCC               GGC^GCC
SmlI          414  CTTGAG               C^T[CT][GA]AG
SpeI           39  ACTAGT               A^CTAGT
SspBI        1577  TGTACA               T^GTACA
TaqI          224  TCGA                 T^CGA
TthHB8I       224  TCGA                 T^CGA
XbaI          753  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AvaII        1398  GGACC                G^G[AT]CC
             1776  GGACC               
BalI         1172  TGGCCA               TGG^CCA
             1533  TGGCCA              
BglI          898  GCCTCCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1489  GCCAAGGTGGC         
BsgI         1138  CTGCAC               GTGCAG(16/14)
             1924  GTGCAG              
BsrI         1200  ACTGG                ACTGG(1/-1)
             1990  CCAGT               
EcoRI         289  GAATTC               G^AATTC
             1690  GAATTC              
FauI         1338  CCCGC                CCCGC(4/6)
             1934  GCGGG               
HaeII        1025  GGCGCT               [GA]GCGC^[CT]
             1334  GGCGCC              
HapII         346  CCGG                 C^CGG
             1967  CCGG                
HgaI          803  GCGTC                GACGC(5/10)
             1195  GCGTC               
HindIII       580  AAGCTT               A^AGCTT
             1852  AAGCTT              
HpaII         346  CCGG                 C^CGG
             1967  CCGG                
MaeII         663  ACGT                 A^CGT
             1154  ACGT                
MluNI        1172  TGGCCA               TGG^CCA
             1533  TGGCCA              
MscI         1172  TGGCCA               TGG^CCA
             1533  TGGCCA              
MspI          346  CCGG                 C^CGG
             1967  CCGG                
PflMI         279  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              642  CCAGCAGCTGG         
ScaI          329  AGTACT               AGT^ACT
             1430  AGTACT              
StuI          868  AGGCCT               AGG^CCT
             1500  AGGCCT              
Van91I        279  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              642  CCAGCAGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       BanI        
BanII       BbsI        BbvI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspHI       BsrDI       BssKI       BssSI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HhaI        HinP1I      HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        PstI        RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XcmI        XhoII       XmnI        
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