Restriction map for pF1KA1868
 (Linear) (Six-base) MAPSORT of: KIAA1868.seq  Check: 3479  from: 1  to: 1995



KIAA1868.orf

 With 182 enzymes: SgfI * 

                        July 31, 2008 16:04  ..

Acc65I G'GTAC_C

Cuts at:      0    1985    1995 
   Size:       1985      10
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     982    1114    1285    1414    1995 
   Size:        982     132     171     129     581

  Fragments arranged by size: 

                 982     581     171     132     129
 
AflIII A'CryG_T

Cuts at:      0    1481    1995 
   Size:       1481     514
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1410    1995 
   Size:       1410     585
 
AleI CACnn'nnGTG

Cuts at:      0    1237    1995 
   Size:       1237     758
 
AlwNI CAG_nnn'CTG

Cuts at:      0     285     648     843    1017    1137    1251    1995 
   Size:        285     363     195     174     120     114     744

  Fragments arranged by size: 

                 744     363     285     195     174     120     114
 
ApaI G_GGCC'C

Cuts at:      0    1314    1995 
   Size:       1314     681
 
ApaLI G'TGCA_C

Cuts at:      0     475    1995 
   Size:        475    1520
 
ApoI r'AATT_y

Cuts at:      0      67     289    1690    1710    1995 
   Size:         67     222    1401      20     285

  Fragments arranged by size: 

                1401     285     222      67      20
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1543    1576    1995 
   Size:       1543      33     419

  Fragments arranged by size: 

                1543     419      33
 
BanI G'GyrC_C

Cuts at:      0     906    1334    1985    1995 
   Size:        906     428     651      10

  Fragments arranged by size: 

                 906     651     428      10
 
BanII G_rGCy'C

Cuts at:      0    1314    1575    1946    1995 
   Size:       1314     261     371      49

  Fragments arranged by size: 

                1314     371     261      49
 
BbeI G_GCGC'C

Cuts at:      0    1338    1995 
   Size:       1338     657
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      80    1640    1903    1995 
   Size:         80    1560     263      92

  Fragments arranged by size: 

                1560     263      92      80
 
BclI T'GATC_A

Cuts at:      0     779    1995 
   Size:        779    1216
 
BglI GCCn_nnn'nGGC

Cuts at:      0     904    1495    1995 
   Size:        904     591     500

  Fragments arranged by size: 

                 904     591     500
 
Bme1580I G_kGCm'C

Cuts at:      0     479    1314    1995 
   Size:        479     835     681

  Fragments arranged by size: 

                 835     681     479
 
BmgBI CAC'GTC

Cuts at:      0    1155    1995 
   Size:       1155     840
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1984    1995 
   Size:       1984      11
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415     500     781    1067    1273    1447    1995 
   Size:        415      85     281     286     206     174     548

  Fragments arranged by size: 

                 548     415     286     281     206     174      85
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     435    1995 
   Size:        435    1560
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     200     336     391    1054    1995 
   Size:        200     136      55     663     941

  Fragments arranged by size: 

                 941     663     200     136      55
 
BsaHI Gr'CG_yC

Cuts at:      0    1335    1995 
   Size:       1335     660
 
BsaWI w'CCGG_w

Cuts at:      0     345    1995 
   Size:        345    1650
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     250     370    1134    1285    1995 
   Size:        250     120     764     151     710

  Fragments arranged by size: 

                 764     710     250     151     120
 
BseYI C'CCAG_C

Cuts at:      0    1826    1995 
   Size:       1826     169
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1123    1945    1995 
   Size:       1123     822      50

  Fragments arranged by size: 

                1123     822      50
 
BsiHKAI G_wGCw'C

Cuts at:      0     242     479    1511    1575    1995 
   Size:        242     237    1032      64     420

  Fragments arranged by size: 

                1032     420     242     237      64
 
BsmI GAATG_Cn'

Cuts at:      0     106    1995 
   Size:        106    1889
 
Bsp1286I G_dGCh'C

Cuts at:      0     242     479    1314    1511    1575    1946    1995 
   Size:        242     237     835     197      64     371      49

  Fragments arranged by size: 

                 835     371     242     237     197      64      49
 
BspHI T'CATG_A

Cuts at:      0      18    1448    1880    1995 
   Size:         18    1430     432     115

  Fragments arranged by size: 

                1430     432     115      18
 
BsrBI CCG'CTC

Cuts at:      0    1934    1995 
   Size:       1934      61
 
BsrDI GCAATG_nn'

Cuts at:      0    1092    1628    1671    1995 
   Size:       1092     536      43     324

  Fragments arranged by size: 

                1092     536     324      43
 
BsrGI T'GTAC_A

Cuts at:      0    1577    1995 
   Size:       1577     418
 
BssSI C'ACGA_G

Cuts at:      0     864    1146    1504    1995 
   Size:        864     282     358     491

  Fragments arranged by size: 

                 864     491     358     282
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1017    1995 
   Size:       1017     978
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     471    1995 
   Size:        471    1524
 
BstYI r'GATC_y

Cuts at:      0     142     180     698    1524    1995 
   Size:        142      38     518     826     471

  Fragments arranged by size: 

                 826     518     471     142      38
 
BtgI C'CryG_G

Cuts at:      0    1862    1995 
   Size:       1862     133
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1366    1995 
   Size:       1366     629
 
BtsI GCAGTG_nn'

Cuts at:      0    1933    1995 
   Size:       1933      62
 
DraI TTT'AAA

Cuts at:      0     489    1995 
   Size:        489    1506
 
EaeI y'GGCC_r

Cuts at:      0    1172    1533    1971    1995 
   Size:       1172     361     438      24

  Fragments arranged by size: 

                1172     438     361      24
 
EarI CTCTTCn'nnn_

Cuts at:      0     502    1712    1766    1995 
   Size:        502    1210      54     229

  Fragments arranged by size: 

                1210     502     229      54
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     155     802    1669    1920    1995 
   Size:        155     647     867     251      75

  Fragments arranged by size: 

                 867     647     251     155      75
 
EcoICRI GAG'CTC

Cuts at:      0    1573    1995 
   Size:       1573     422
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415     500     781     982    1067    1114    1273    1285 
   Size:        415      85     281     201      85      47     159      12

Cuts at:   1285    1414    1447    1995 
   Size:        129      33     548

  Fragments arranged by size: 

                 548     415     281     201     159     129      85      85
                  47      33      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1310    1311    1398    1995 
   Size:       1310       1      87     597

  Fragments arranged by size: 

                1310     597      87       1
 
EcoRI G'AATT_C

Cuts at:      0     289    1690    1995 
   Size:        289    1401     305

  Fragments arranged by size: 

                1401     305     289
 
EcoRV GAT'ATC

Cuts at:      0    1284    1995 
   Size:       1284     711
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     237    1995 
   Size:        237    1758
 
HaeII r_GCGC'y

Cuts at:      0    1029    1338    1995 
   Size:       1029     309     657

  Fragments arranged by size: 

                1029     657     309
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     145     177     253     285    1396    1428    1995 
   Size:        145      32      76      32    1111      32     567

  Fragments arranged by size: 

                1111     567     145      76      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     580    1852    1995 
   Size:        580    1272     143

  Fragments arranged by size: 

                1272     580     143
 
KasI G'GCGC_C

Cuts at:      0    1334    1995 
   Size:       1334     661
 
KpnI G_GTAC'C

Cuts at:      0    1989    1995 
   Size:       1989       6
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     515    1995 
   Size:        515    1480
 
MscI TGG'CCA

Cuts at:      0    1174    1535    1995 
   Size:       1174     361     460

  Fragments arranged by size: 

                1174     460     361
 
MslI CAynn'nnrTG

Cuts at:      0    1237    1995 
   Size:       1237     758
 
MspA1I CmG'CkG

Cuts at:      0     648     770    1341    1995 
   Size:        648     122     571     654

  Fragments arranged by size: 

                 654     648     571     122
 
NarI GG'CG_CC

Cuts at:      0    1335    1995 
   Size:       1335     660
 
NspI r_CATG'y

Cuts at:      0    1485    1995 
   Size:       1485     510
 
PciI A'CATG_T

Cuts at:      0    1481    1995 
   Size:       1481     514
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     285     648    1995 
   Size:        285     363    1347

  Fragments arranged by size: 

                1347     363     285
 
PfoI T'CCnGG_A

Cuts at:      0     500     508    1044    1995 
   Size:        500       8     536     951

  Fragments arranged by size: 

                 951     536     500       8
 
PpuMI rG'GwC_Cy

Cuts at:      0    1398    1995 
   Size:       1398     597
 
PspOMI G'GGCC_C

Cuts at:      0    1310    1995 
   Size:       1310     685
 
PstI C_TGCA'G

Cuts at:      0    1247    1319    1346    1995 
   Size:       1247      72      27     649

  Fragments arranged by size: 

                1247     649      72      27
 
PvuII CAG'CTG

Cuts at:      0     648    1995 
   Size:        648    1347
 
SacI G_AGCT'C

Cuts at:      0    1575    1995 
   Size:       1575     420
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1712    1995 
   Size:       1712     283
 
ScaI AGT'ACT

Cuts at:      0     331    1432    1995 
   Size:        331    1101     563

  Fragments arranged by size: 

                1101     563     331
 
SfcI C'TryA_G

Cuts at:      0    1243    1315    1342    1995 
   Size:       1243      72      27     653

  Fragments arranged by size: 

                1243     653      72      27
 
SfoI GGC'GCC

Cuts at:      0    1336    1995 
   Size:       1336     659
 
SmlI C'TyrA_G

Cuts at:      0     414    1995 
   Size:        414    1581
 
SpeI A'CTAG_T

Cuts at:      0      39    1995 
   Size:         39    1956
 
StuI AGG'CCT

Cuts at:      0     870    1502    1995 
   Size:        870     632     493

  Fragments arranged by size: 

                 870     632     493
 
StyI C'CwwG_G

Cuts at:      0     902     944    1490    1995 
   Size:        902      42     546     505

  Fragments arranged by size: 

                 902     546     505      42
 
TatI w'GTAC_w

Cuts at:      0     329    1166    1430    1577    1995 
   Size:        329     837     264     147     418

  Fragments arranged by size: 

                 837     418     329     264     147
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1026    1058    1995 
   Size:       1026      32     937

  Fragments arranged by size: 

                1026     937      32
 
XbaI T'CTAG_A

Cuts at:      0     753    1995 
   Size:        753    1242
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     721    1152    1869    1995 
   Size:        721     431     717     126

  Fragments arranged by size: 

                 721     717     431     126
 
XmnI GAAnn'nnTTC

Cuts at:      0     217     542    1000    1995 
   Size:        217     325     458     995

  Fragments arranged by size: 

                 995     458     325     217
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AhdI     AleI    AlwNI     ApaI    ApaLI
     ApoI     BaeI     BanI    BanII     BbeI     BbsI     BclI     BglI
 Bme1580I    BmgBI     BmrI     BpmI    BpuEI     BsaI    BsaHI    BsaWI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrBI
    BsrDI    BsrGI    BssSI   BstAPI    BstXI    BstYI     BtgI    BtgZI
     BtsI     DraI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI    HaeII    Hin4I  HindIII     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NarI     NspI     PciI    PflMI
     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI     SapI     ScaI
     SfcI     SfoI     SmlI     SpeI     StuI     StyI     TatI     TstI
     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AlfI
     AloI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI    BbvCI
     BcgI    BciVI    BfrBI    BglII     BlpI     BmtI     BplI   Bpu10I
    BsaAI    BsaBI    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BspMI
    BsrFI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII     DrdI     EagI    EcoNI     FseI     FspI    FspAI   HincII
     HpaI     MfeI     MluI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SphI     SrfI     SspI     SwaI    TaqII  Tth111I     XhoI     XmaI
     ZraI
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