Restriction map for pF1KA1866
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# NO CUT ENZYMES (   64 enzymes) #
##################################

AccIII      AclI        AgeI        AscI        AseI        
AsnI        AspI        AviII       AvrII       BclI        
BlnI        BseAI       BsgI        BsiWI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrGI       
Bst1107I    BstAPI      BstBI       BstXI       BstZ17I     
ClaI        CpoI        EcoRI       EcoRV       FbaI        
FseI        FspI        HpaI        MfeI        MroI        
MunI        NdeI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SapI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
SwaI        Tth111I     XbaI        XhoI        


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# ONE CUT ENZYMES (   34 enzymes) #
###################################

AatII        4341  GACGTC               GACGT^C
Acc65I       3767  GGTACC               G^GTACC
AflII        2379  CTTAAG               C^TTAAG
Alw44I       1714  GTGCAC               G^TGCAC
ApaLI        1714  GTGCAC               G^TGCAC
Asp718I      3767  GGTACC               G^GTACC
BbvCI        1790  CCTCAGC              CCTCAGC(-5/-2)
BciVI        2276  GTATCC               GTATCC(6/5)
BfrI         2379  CTTAAG               C^TTAAG
BglII        3834  AGATCT               A^GATCT
BlpI         2265  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     2265  GCTTAGC              GC^T[ACGT]AGC
BsaBI        2458  GATACACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         341  TCCGGT               [AT]^CCGG[AT]
BsrFI        1740  GCCGGC               [GA]^CCGG[CT]
BssSI        4806  CACGAG               CACGAG(-5/-1)
CelII        2265  GCTTAGC              GC^T[ACGT]AGC
Cfr10I       1740  GCCGGC               [GA]^CCGG[CT]
DrdI          637  GACATCAGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2649  CGGCCG               C^GGCCG
EclXI        2649  CGGCCG               C^GGCCG
Eco52I       2649  CGGCCG               C^GGCCG
EcoT22I      3562  ATGCAT               ATGCA^T
KpnI         3767  GGTACC               GGTAC^C
KspI         3490  CCGCGG               CCGC^GG
MamI         2458  GATACACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         1740  GCCGGC               GCC^GGC
NgoMIV       1740  GCCGGC               G^CCGGC
NheI          507  GCTAGC               G^CTAGC
NsiI         3562  ATGCAT               ATGCA^T
SacII        3490  CCGCGG               CCGC^GG
ScaI         2477  AGTACT               AGT^ACT
SspI         1191  AATATT               AAT^ATT
StuI         4304  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflIII       1523  ACGCGT               A^C[GA][CT]GT
             2579  ACGCGT              
AocI         1350  CCTGAGG              CC^T[ACGT]AGG
             2467  CCTCAGG             
Aor51HI      1422  AGCGCT               AGC^GCT
             3448  AGCGCT              
Asp700I       599  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3232  GAAGACCTTC          
BcgI         4025  CGACGCCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2903  GCACAGAAGTCG        
BfaI          508  CTAG                 C^TAG
             3808  CTAG                
BsiEI        2649  CGGCCG               CG[GA][CT]^CG
             3433  CGACCG              
BsmI          111  GAATGC               GAATGC(1/-1)
             3753  GAATGC              
BsrBI        2796  CCGCTC               CCGCTC(-3/-3)
             1994  GAGCGG              
BsrDI          66  CATTGC               GCAATG(2/0)
             5128  CATTGC              
BstEII       2732  GGTCACC              G^GT[ACGT]ACC
             3022  GGTGACC             
BstPI        2732  GGTCACC              G^GT[ACGT]ACC
             3022  GGTGACC             
Bsu36I       1350  CCTGAGG              CC^T[ACGT]AGG
             2467  CCTCAGG             
DraI         3217  TTTAAA               TTT^AAA
             4819  TTTAAA              
DraIII        857  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1375  CACTCGGTG           
Eco47III     1422  AGCGCT               AGC^GCT
             3448  AGCGCT              
Eco81I       1350  CCTGAGG              CC^T[ACGT]AGG
             2467  CCTCAGG             
EcoO65I      2732  GGTCACC              G^GT[ACGT]ACC
             3022  GGTGACC             
HincII       1263  GTCGAC               GT[CT]^[GA]AC
             2205  GTCGAC              
HindII       1263  GTCGAC               GT[CT]^[GA]AC
             2205  GTCGAC              
HindIII      2263  AAGCTT               A^AGCTT
             4760  AAGCTT              
MaeI          508  CTAG                 C^TAG
             3808  CTAG                
MluI         1523  ACGCGT               A^CGCGT
             2579  ACGCGT              
PshAI        1977  GACGCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2722  GACGCGGGTC          
PvuII        2963  CAGCTG               CAG^CTG
             5106  CAGCTG              
SalI         1263  GTCGAC               G^TCGAC
             2205  GTCGAC              
SphI         3564  GCATGC               GCATG^C
             4605  GCATGC              
XmnI          599  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3232  GAAGACCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  132 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AhdI        AluI        AlwI        AlwNI       
ApaI        ApoI        AspEI       AspHI       AvaI        
AvaII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvI        BcnI        
BglI        BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssHII      
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        Eam1105I    EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NarI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PmaCI       
PmlI        PpuMI       PstI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmaI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI        
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