Restriction map for pF1KA1866
 (Linear) (Six-base) MAPSORT of: KIAA1866.seq  Check: 5636  from: 1  to: 5319



KIAA1866.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2979    3007    5319 
   Size:       2979      28    2312

  Fragments arranged by size: 

                2979    2312      28
 
AatII G_ACGT'C

Cuts at:      0    4345    5319 
   Size:       4345     974
 
AccI GT'mk_AC

Cuts at:      0      45     575    1264    1365    2206    5319 
   Size:         45     530     689     101     841    3113

  Fragments arranged by size: 

                3113     841     689     530     101      45
 
Acc65I G'GTAC_C

Cuts at:      0    3767    5319 
   Size:       3767    1552
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1243    4271    4451    4600    5319 
   Size:       1243    3028     180     149     719

  Fragments arranged by size: 

                3028    1243     719     180     149
 
AfeI AGC'GCT

Cuts at:      0    1424    3450    5319 
   Size:       1424    2026    1869

  Fragments arranged by size: 

                2026    1869    1424
 
AflII C'TTAA_G

Cuts at:      0    2379    5319 
   Size:       2379    2940
 
AflIII A'CryG_T

Cuts at:      0    1523    2579    5319 
   Size:       1523    1056    2740

  Fragments arranged by size: 

                2740    1523    1056
 
AhdI GACnn_n'nnGTC

Cuts at:      0     360    3030    3240    5319 
   Size:        360    2670     210    2079

  Fragments arranged by size: 

                2670    2079     360     210
 
AleI CACnn'nnGTG

Cuts at:      0    4627    5319 
   Size:       4627     692
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2428    2460    4735    4767    5319 
   Size:       2428      32    2275      32     552

  Fragments arranged by size: 

                2428    2275     552      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     829     913     931    1006    2076    5319 
   Size:        829      84      18      75    1070    3243

  Fragments arranged by size: 

                3243    1070     829      84      75      18
 
ApaI G_GGCC'C

Cuts at:      0    1413    2085    2658    2934    5319 
   Size:       1413     672     573     276    2385

  Fragments arranged by size: 

                2385    1413     672     573     276
 
ApaLI G'TGCA_C

Cuts at:      0    1714    5319 
   Size:       1714    3605
 
ApoI r'AATT_y

Cuts at:      0     838    4333    4438    5319 
   Size:        838    3495     105     881

  Fragments arranged by size: 

                3495     881     838     105
 
AvaI C'yCGr_G

Cuts at:      0    1298    2219    2363    2703    3098    4050    5319 
   Size:       1298     921     144     340     395     952    1269

  Fragments arranged by size: 

                1298    1269     952     921     395     340     144
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    4274    4307    5277    5310    5319 
   Size:       4274      33     970      33       9

  Fragments arranged by size: 

                4274     970      33      33       9
 
BamHI G'GATC_C

Cuts at:      0     687    4232    4937    5319 
   Size:        687    3545     705     382

  Fragments arranged by size: 

                3545     705     687     382
 
BanI G'GyrC_C

Cuts at:      0     378    1209    1488    1537    1636    3475    3767    4411 
   Size:        378     831     279      49      99    1839     292     644

Cuts at:   4411    5319 
   Size:        908

  Fragments arranged by size: 

                1839     908     831     644     378     292     279      99
                  49
 
BanII G_rGCy'C

Cuts at:      0    1107    1413    1621    1867    2085    2226    2658    2833 
   Size:       1107     306     208     246     218     141     432     175

Cuts at:   2833    2934    3466    4225    5319 
   Size:        101     532     759    1094

  Fragments arranged by size: 

                1107    1094     759     532     432     306     246     218
                 208     175     141     101
 
BbeI G_GCGC'C

Cuts at:      0    1213    1492    1640    4415    5319 
   Size:       1213     279     148    2775     904

  Fragments arranged by size: 

                2775    1213     904     279     148
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     885    2045    3236    3239    4736    5129    5319 
   Size:        885    1160    1191       3    1497     393     190

  Fragments arranged by size: 

                1497    1191    1160     885     393     190       3
 
BbvCI CC'TCA_GC

Cuts at:      0    1791    5319 
   Size:       1791    3528
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2892    2926    4014    4048    5319 
   Size:       2892      34    1088      34    1271

  Fragments arranged by size: 

                2892    1271    1088      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2287    5319 
   Size:       2287    3032
 
BfrBI ATG'CAT

Cuts at:      0    3564    5319 
   Size:       3564    1755
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1030    1240    1741    2497    2959    3077    3261    5319 
   Size:       1030     210     501     756     462     118     184    2058

  Fragments arranged by size: 

                2058    1030     756     501     462     210     184     118
            
 
BglII A'GATC_T

Cuts at:      0    3834    5319 
   Size:       3834    1485
 
BlpI GC'TnA_GC

Cuts at:      0    2266    5319 
   Size:       2266    3053
 
Bme1580I G_kGCm'C

Cuts at:      0     317    1413    1540    1718    2085    2216    2658    2934 
   Size:        317    1096     127     178     367     131     442     276

Cuts at:   2934    3478    5319 
   Size:        544    1841

  Fragments arranged by size: 

                1841    1096     544     442     367     317     276     178
                 131     127
 
BmgBI CAC'GTC

Cuts at:      0    2690    2786    3423    5319 
   Size:       2690      96     637    1896

  Fragments arranged by size: 

                2690    1896     637      96
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1286    2618    3081    4677    5319 
   Size:       1286    1332     463    1596     642

  Fragments arranged by size: 

                1596    1332    1286     642     463
 
BmtI G_CTAG'C

Cuts at:      0     511    5319 
   Size:        511    4808
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     141    1104    2860    3383    5319 
   Size:        141     963    1756     523    1936

  Fragments arranged by size: 

                1936    1756     963     523     141
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     413    1091    2175    2331    2817    2842    3380 
   Size:        254     159     678    1084     156     486      25     538

Cuts at:   3380    5319 
   Size:       1939

  Fragments arranged by size: 

                1939    1084     678     538     486     254     159     156
                  25
 
Bpu10I CC'TnA_GC

Cuts at:      0    1079    1791    1861    2261    2271    2501    5319 
   Size:       1079     712      70     400      10     230    2818

  Fragments arranged by size: 

                2818    1079     712     400     230      70      10
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1788    3692    4742    5319 
   Size:       1788    1904    1050     577

  Fragments arranged by size: 

                1904    1788    1050     577
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     537    1989    2150    3183    4875    5304    5319 
   Size:        537    1452     161    1033    1692     429      15

  Fragments arranged by size: 

                1692    1452    1033     537     429     161      15
 
BsaAI yAC'GTr

Cuts at:      0      17    2334    4479    5006    5319 
   Size:         17    2317    2145     527     313

  Fragments arranged by size: 

                2317    2145     527     313      17
 
BsaBI GATnn'nnATC

Cuts at:      0    2462    5319 
   Size:       2462    2857
 
BsaHI Gr'CG_yC

Cuts at:      0    1210    1489    1637    1901    3296    4027    4092    4342 
   Size:       1210     279     148     264    1395     731      65     250

Cuts at:   4342    4412    5319 
   Size:         70     907

  Fragments arranged by size: 

                1395    1210     907     731     279     264     250     148
                  70      65
 
BsaWI w'CCGG_w

Cuts at:      0     341    5319 
   Size:        341    4978
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     912     942    3571    3601    4679    4709    5319 
   Size:        912      30    2629      30    1078      30     610

  Fragments arranged by size: 

                2629    1078     912     610      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1261    1297    1300    1938    2305    2334    2472    2666 
   Size:       1261      36       3     638     367      29     138     194

Cuts at:   2666    2796    2799    3208    3211    3214    3827    5319 
   Size:        130       3     409       3       3     613    1492

  Fragments arranged by size: 

                1492    1261     638     613     409     367     194     138
                 130      36      29       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1223    1290    1646    1669    1850    1905    2369    2693 
   Size:       1223      67     356      23     181      55     464     324

Cuts at:   2693    2741    3035    3067    3106    3167    4601    4963    5108 
   Size:         48     294      32      39      61    1434     362     145

Cuts at:   5108    5319 
   Size:        211

  Fragments arranged by size: 

                1434    1223     464     362     356     324     294     211
                 181     145      67      61      55      48      39      32
                  23
 
BsiEI CG_ry'CG

Cuts at:      0    2652    3436    5319 
   Size:       2652     784    1883

  Fragments arranged by size: 

                2652    1883     784
 
BsiHKAI G_wGCw'C

Cuts at:      0    1107    1718    1867    2521    2833    4225    4516    5319 
   Size:       1107     611     149     654     312    1392     291     803

  Fragments arranged by size: 

                1392    1107     803     654     611     312     291     149
            
 
BsmI GAATG_Cn'

Cuts at:      0     117    3759    5319 
   Size:        117    3642    1560

  Fragments arranged by size: 

                3642    1560     117
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1959    2279    2714    4333    5319 
   Size:       1959     320     435    1619     986

  Fragments arranged by size: 

                1959    1619     986     435     320
 
Bsp1286I G_dGCh'C

Cuts at:      0     317    1107    1413    1540    1621    1718    1867    2085 
   Size:        317     790     306     127      81      97     149     218

Cuts at:   2085    2216    2226    2521    2658    2833    2934    3466    3478 
   Size:        131      10     295     137     175     101     532      12

Cuts at:   3478    4225    4516    5319 
   Size:        747     291     803

  Fragments arranged by size: 

                 803     790     747     532     317     306     295     291
                 218     175     149     137     131     127     101      97
                  81      12      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1971    2144    2979    3007    4601    5319 
   Size:       1971     173     835      28    1594     718

  Fragments arranged by size: 

                1971    1594     835     718     173      28
 
BsrBI CCG'CTC

Cuts at:      0    1996    2798    5319 
   Size:       1996     802    2521

  Fragments arranged by size: 

                2521    1996     802
 
BsrDI GCAATG_nn'

Cuts at:      0      65    5127    5319 
   Size:         65    5062     192

  Fragments arranged by size: 

                5062     192      65
 
BsrFI r'CCGG_y

Cuts at:      0    1740    5319 
   Size:       1740    3579
 
BssHII G'CGCG_C

Cuts at:      0    1608    2947    2949    3504    5319 
   Size:       1608    1339       2     555    1815

  Fragments arranged by size: 

                1815    1608    1339     555       2
 
BssSI C'ACGA_G

Cuts at:      0    4806    5319 
   Size:       4806     513
 
BstEII G'GTnAC_C

Cuts at:      0    2732    3022    5319 
   Size:       2732     290    2297

  Fragments arranged by size: 

                2732    2297     290
 
BstYI r'GATC_y

Cuts at:      0     687    1254    3834    4232    4937    5319 
   Size:        687     567    2580     398     705     382

  Fragments arranged by size: 

                2580     705     687     567     398     382
 
Bsu36I CC'TnA_GG

Cuts at:      0    1351    2468    5319 
   Size:       1351    1117    2851

  Fragments arranged by size: 

                2851    1351    1117
 
BtgI C'CryG_G

Cuts at:      0    1821    2019    2063    2280    2849    3490    3851    4625 
   Size:       1821     198      44     217     569     641     361     774

Cuts at:   4625    4634    4980    5319 
   Size:          9     346     339

  Fragments arranged by size: 

                1821     774     641     569     361     346     339     217
                 198      44       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3321    5319 
   Size:       3321    1998
 
BtsI GCAGTG_nn'

Cuts at:      0    1409    4061    4283    4376    5319 
   Size:       1409    2652     222      93     943

  Fragments arranged by size: 

                2652    1409     943     222      93
 
DraI TTT'AAA

Cuts at:      0    3219    4821    5319 
   Size:       3219    1602     498

  Fragments arranged by size: 

                3219    1602     498
 
DraIII CAC_nnn'GTG

Cuts at:      0     862    1380    5319 
   Size:        862     518    3939

  Fragments arranged by size: 

                3939     862     518
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     643    5319 
   Size:        643    4676
 
EaeI y'GGCC_r

Cuts at:      0     447     764     949    1371    1466    1654    2649    3487 
   Size:        447     317     185     422      95     188     995     838

Cuts at:   3487    3516    3908    4631    5319 
   Size:         29     392     723     688

  Fragments arranged by size: 

                 995     838     723     688     447     422     392     317
                 188     185      95      29
 
EagI C'GGCC_G

Cuts at:      0    2649    5319 
   Size:       2649    2670
 
EarI CTCTTCn'nnn_

Cuts at:      0     605    1962    3273    3314    3800    4295    4579    5109 
   Size:        605    1357    1311      41     486     495     284     530

Cuts at:   5109    5319 
   Size:        210

  Fragments arranged by size: 

                1357    1311     605     530     495     486     284     210
                  41
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1048    2060    2071    5319 
   Size:       1048    1012      11    3248

  Fragments arranged by size: 

                3248    1048    1012      11
 
EcoICRI GAG'CTC

Cuts at:      0    1105    1865    2831    4223    5319 
   Size:       1105     760     966    1392    1096

  Fragments arranged by size: 

                1392    1105    1096     966     760
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     413    1091    1243    2175    2331    2817    2842 
   Size:        254     159     678     152     932     156     486      25

Cuts at:   2842    3380    4271    4451    4600    5319 
   Size:        538     891     180     149     719

  Fragments arranged by size: 

                 932     891     719     678     538     486     254     180
                 159     156     152     149      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     136    2346    3313    3353    5319 
   Size:        136    2210     967      40    1966

  Fragments arranged by size: 

                2210    1966     967     136      40
 
EcoO109I rG'GnC_Cy

Cuts at:      0      21    1164    1409    1751    2173    2655    2707    2931 
   Size:         21    1143     245     342     422     482      52     224

Cuts at:   2931    3032    3102    3413    3845    4181    4913    5167    5319 
   Size:        101      70     311     432     336     732     254     152

  Fragments arranged by size: 

                1143     732     482     432     422     342     336     311
                 254     245     224     152     101      70      52      21
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1473    1656    3253    5319 
   Size:       1473     183    1597    2066

  Fragments arranged by size: 

                2066    1597    1473     183
 
HaeII r_GCGC'y

Cuts at:      0    1213    1426    1492    1640    3121    3452    4415    5319 
   Size:       1213     213      66     148    1481     331     963     904

  Fragments arranged by size: 

                1481    1213     963     904     331     213     148      66
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     346     378    1246    1278    1693    1725    3016    3048 
   Size:        346      32     868      32     415      32    1291      32

Cuts at:   3048    3226    3258    4249    4281    4679    4711    5319 
   Size:        178      32     991      32     398      32     608

  Fragments arranged by size: 

                1291     991     868     608     415     398     346     178
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1265    2207    5319 
   Size:       1265     942    3112

  Fragments arranged by size: 

                3112    1265     942
 
HindIII A'AGCT_T

Cuts at:      0    2263    4760    5319 
   Size:       2263    2497     559

  Fragments arranged by size: 

                2497    2263     559
 
KasI G'GCGC_C

Cuts at:      0    1209    1488    1636    4411    5319 
   Size:       1209     279     148    2775     908

  Fragments arranged by size: 

                2775    1209     908     279     148
 
KpnI G_GTAC'C

Cuts at:      0    3771    5319 
   Size:       3771    1548
 
MluI A'CGCG_T

Cuts at:      0    1523    2579    5319 
   Size:       1523    1056    2740

  Fragments arranged by size: 

                2740    1523    1056
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    3110    4120    5319 
   Size:       3110    1010    1199

  Fragments arranged by size: 

                3110    1199    1010
 
MscI TGG'CCA

Cuts at:      0     766     951    1468    3518    3910    5319 
   Size:        766     185     517    2050     392    1409

  Fragments arranged by size: 

                2050    1409     766     517     392     185
 
MslI CAynn'nnrTG

Cuts at:      0     106    2624    3535    4003    4199    4624    4627    5153 
   Size:        106    2518     911     468     196     425       3     526

Cuts at:   5153    5319 
   Size:        166

  Fragments arranged by size: 

                2518     911     526     468     425     196     166     106
                   3
 
MspA1I CmG'CkG

Cuts at:      0    2965    3492    3954    5108    5319 
   Size:       2965     527     462    1154     211

  Fragments arranged by size: 

                2965    1154     527     462     211
 
NaeI GCC'GGC

Cuts at:      0    1742    5319 
   Size:       1742    3577
 
NarI GG'CG_CC

Cuts at:      0    1210    1489    1637    4412    5319 
   Size:       1210     279     148    2775     907

  Fragments arranged by size: 

                2775    1210     907     279     148
 
NcoI C'CATG_G

Cuts at:      0    1821    2280    4980    5319 
   Size:       1821     459    2700     339

  Fragments arranged by size: 

                2700    1821     459     339
 
NgoMIV G'CCGG_C

Cuts at:      0    1740    5319 
   Size:       1740    3579
 
NheI G'CTAG_C

Cuts at:      0     507    5319 
   Size:        507    4812
 
NsiI A_TGCA'T

Cuts at:      0    3566    5319 
   Size:       3566    1753
 
NspI r_CATG'y

Cuts at:      0    3049    3568    4609    5319 
   Size:       3049     519    1041     710

  Fragments arranged by size: 

                3049    1041     710     519
 
PasI CC'CwG_GG

Cuts at:      0    1483    1533    2231    2880    3281    3410    4177    5319 
   Size:       1483      50     698     649     401     129     767    1142

  Fragments arranged by size: 

                1483    1142     767     698     649     401     129      50
            
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     958    1380    1822    2991    3880    5139    5319 
   Size:        958     422     442    1169     889    1259     180

  Fragments arranged by size: 

                1259    1169     958     889     442     422     180
 
PfoI T'CCnGG_A

Cuts at:      0     276    1972    5319 
   Size:        276    1696    3347

  Fragments arranged by size: 

                3347    1696     276
 
PmlI CAC'GTG

Cuts at:      0      17    2334    5006    5319 
   Size:         17    2317    2672     313

  Fragments arranged by size: 

                2672    2317     313      17
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    5154    5186    5319 
   Size:       5154      32     133

  Fragments arranged by size: 

                5154     133      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      21    1164    1751    2173    2707    3032    3102    3845 
   Size:         21    1143     587     422     534     325      70     743

Cuts at:   3845    4181    5319 
   Size:        336    1138

  Fragments arranged by size: 

                1143    1138     743     587     534     422     336     325
                  70      21
 
PshAI GACnn'nnGTC

Cuts at:      0    1981    2726    5319 
   Size:       1981     745    2593

  Fragments arranged by size: 

                2593    1981     745
 
PsiI TTA'TAA

Cuts at:      0     779    3626    5319 
   Size:        779    2847    1693

  Fragments arranged by size: 

                2847    1693     779
 
PspOMI G'GGCC_C

Cuts at:      0    1409    2081    2654    2930    5319 
   Size:       1409     672     573     276    2389

  Fragments arranged by size: 

                2389    1409     672     573     276
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    5002    5034    5319 
   Size:       5002      32     285

  Fragments arranged by size: 

                5002     285      32
 
PstI C_TGCA'G

Cuts at:      0     183     542    1404    2186    4290    5319 
   Size:        183     359     862     782    2104    1029

  Fragments arranged by size: 

                2104    1029     862     782     359     183
 
PvuII CAG'CTG

Cuts at:      0    2965    5108    5319 
   Size:       2965    2143     211

  Fragments arranged by size: 

                2965    2143     211
 
SacI G_AGCT'C

Cuts at:      0    1107    1867    2833    4225    5319 
   Size:       1107     760     966    1392    1094

  Fragments arranged by size: 

                1392    1107    1094     966     760
 
SacII CC_GC'GG

Cuts at:      0    3493    5319 
   Size:       3493    1826
 
SalI G'TCGA_C

Cuts at:      0    1263    2205    5319 
   Size:       1263     942    3114

  Fragments arranged by size: 

                3114    1263     942
 
SanDI GG'GwC_CC

Cuts at:      0      21    2707    3102    3845    5319 
   Size:         21    2686     395     743    1474

  Fragments arranged by size: 

                2686    1474     743     395      21
 
ScaI AGT'ACT

Cuts at:      0    2479    5319 
   Size:       2479    2840
 
SfcI C'TryA_G

Cuts at:      0     179     538     576     968    1400    2182    4286    5091 
   Size:        179     359      38     392     432     782    2104     805

Cuts at:   5091    5319 
   Size:        228

  Fragments arranged by size: 

                2104     805     782     432     392     359     228     179
                  38
 
SfoI GGC'GCC

Cuts at:      0    1211    1490    1638    4413    5319 
   Size:       1211     279     148    2775     906

  Fragments arranged by size: 

                2775    1211     906     279     148
 
SmaI CCC'GGG

Cuts at:      0    1300    2365    2705    3100    5319 
   Size:       1300    1065     340     395    2219

  Fragments arranged by size: 

                2219    1300    1065     395     340
 
SmlI C'TyrA_G

Cuts at:      0    1803    2379    3707    4757    5319 
   Size:       1803     576    1328    1050     562

  Fragments arranged by size: 

                1803    1328    1050     576     562
 
SphI G_CATG'C

Cuts at:      0    3568    4609    5319 
   Size:       3568    1041     710

  Fragments arranged by size: 

                3568    1041     710
 
SspI AAT'ATT

Cuts at:      0    1193    5319 
   Size:       1193    4126
 
StuI AGG'CCT

Cuts at:      0    4306    5319 
   Size:       4306    1013
 
StyI C'CwwG_G

Cuts at:      0    1146    1160    1631    1821    1877    2280    2495    2954 
   Size:       1146      14     471     190      56     403     215     459

Cuts at:   2954    3314    4184    4980    5319 
   Size:        360     870     796     339

  Fragments arranged by size: 

                1146     870     796     471     459     403     360     339
                 215     190      56      14
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      42     672    1345    1546    1864    2720    3165    3493 
   Size:         42     630     673     201     318     856     445     328

Cuts at:   3493    4000    4857    5319 
   Size:        507     857     462

  Fragments arranged by size: 

                 857     856     673     630     507     462     445     328
                 318     201      42
 
TatI w'GTAC_w

Cuts at:      0     161     731    2477    5319 
   Size:        161     570    1746    2842

  Fragments arranged by size: 

                2842    1746     570     161
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1510    1542    1878    1910    2236    2268    2323    2355 
   Size:       1510      32     336      32     326      32      55      32

Cuts at:   2355    3412    3444    4077    4109    5319 
   Size:       1057      32     633      32    1210

  Fragments arranged by size: 

                1510    1210    1057     633     336     326      55      32
                  32      32      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      48    3781    3834    5319 
   Size:         48    3733      53    1485

  Fragments arranged by size: 

                3733    1485      53      48
 
XmaI C'CCGG_G

Cuts at:      0    1298    2363    2703    3098    5319 
   Size:       1298    1065     340     395    2221

  Fragments arranged by size: 

                2221    1298    1065     395     340
 
XmnI GAAnn'nnTTC

Cuts at:      0     603    3236    5319 
   Size:        603    2633    2083

  Fragments arranged by size: 

                2633    2083     603
 
ZraI GAC'GTC

Cuts at:      0    4343    5319 
   Size:       4343     976
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI
     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NsiI     NspI     PasI    PflMI     PfoI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI     PstI    PvuII
     SacI    SacII     SalI    SanDI     ScaI     SfcI     SfoI     SmaI
     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI     AgeI     AlfI     AscI     AseI    AsiSI    AvrII     BclI
     BsgI    BsiWI    BspEI    BspHI    BsrGI   BstAPI    BstBI    BstXI
  BstZ17I     ClaI    CspCI    EcoRI    EcoRV     FseI     FspI    FspAI
     HpaI     MfeI     NdeI     NotI     NruI     PacI     PciI     PmeI
    PspXI     PvuI    RsrII     SapI     SbfI    SexAI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SwaI  Tth111I     XbaI     XhoI
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