Restriction map for pF1KA1853
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BbeI        BbrPI       BbsI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BsaWI       BseAI       
BsgI        BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BsrDI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
DrdI        Eam1105I    Eco47III    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KasI        KpnI        MamI        
MfeI        MluI        MroI        MseI        MslI        
MunI        NarI        NcoI        NdeI        NheI        
NotI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tru9I       Tsp509I     Tth111I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        1457  GACGTC               GACGT^C
AcyI         1457  GACGTC               G[GA]^CG[CT]C
AflIII        869  ACACGT               A^C[GA][CT]GT
AlwNI         193  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       993  GAACTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI        1398  GGATCC               G^GATCC
BbvCI        1587  CCTCAGC              CCTCAGC(-5/-2)
BcgI           89  GCATCATTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI        1457  GACGTC               G[GA]^CG[CT]C
BsaI          908  GGTCTC               GGTCTC(1/5)
BsiEI         621  CGGCCG               CG[GA][CT]^CG
BspHI         285  TCATGA               T^CATGA
BspMI         434  GCAGGT               ACCTGC(4/8)
BstXI         738  CCAGATGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1398  GGATCC               [GA]^GATC[CT]
DsaI          618  CCGCGG               C^C[GA][CT]GG
EagI          621  CGGCCG               C^GGCCG
EclXI         621  CGGCCG               C^GGCCG
Eco52I        621  CGGCCG               C^GGCCG
HaeII        1484  AGCGCC               [GA]GCGC^[CT]
Hin1I        1457  GACGTC               G[GA]^CG[CT]C
HincII        728  GTCAAC               GT[CT]^[GA]AC
HindII        728  GTCAAC               GT[CT]^[GA]AC
KspI          618  CCGCGG               CCGC^GG
MflI         1398  GGATCC               [GA]^GATC[CT]
NaeI          524  GCCGGC               GCC^GGC
NgoMIV        524  GCCGGC               G^CCGGC
NruI          832  TCGCGA               TCG^CGA
PflMI         221  CCAACAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI          285  TCATGA               T^CATGA
SacII         618  CCGCGG               CCGC^GG
TaqI          519  TCGA                 T^CGA
TthHB8I       519  TCGA                 T^CGA
Van91I        221  CCAACAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          203  CCACCGAGCCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1398  GGATCC               [GA]^GATC[CT]
XmnI          993  GAACTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AocI          631  CCTGAGG              CC^T[ACGT]AGG
              700  CCTGAGG             
BalI           68  TGGCCA               TGG^CCA
              200  TGGCCA              
BlpI          899  GCTCAGC              GC^T[ACGT]AGC
             1802  GCTCAGC             
Bpu1102I      899  GCTCAGC              GC^T[ACGT]AGC
             1802  GCTCAGC             
BsmBI        1455  GAGACG               CGTCTC(1/5)
             1677  GAGACG              
BsrFI         524  GCCGGC               [GA]^CCGG[CT]
              623  GCCGGT              
BssSI        1695  CACGAG               CACGAG(-5/-1)
             1824  CACGAG              
Bsu36I        631  CCTGAGG              CC^T[ACGT]AGG
              700  CCTGAGG             
CelII         899  GCTCAGC              GC^T[ACGT]AGC
             1802  GCTCAGC             
Cfr10I        524  GCCGGC               [GA]^CCGG[CT]
              623  GCCGGT              
Eco81I        631  CCTGAGG              CC^T[ACGT]AGG
              700  CCTGAGG             
EcoNI         631  CCTGAGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              684  CCTCTGCAAGG         
EcoT14I       127  CCAAGG               C^C[AT][AT]GG
              212  CCTTGG              
HgaI          431  GACGC                GACGC(5/10)
             1449  GCGTC               
MaeIII       1086  GTCAC                ^GT[ACGT]AC
             1205  GTCAC               
MluNI          68  TGGCCA               TGG^CCA
              200  TGGCCA              
MscI           68  TGGCCA               TGG^CCA
              200  TGGCCA              
PshAI         431  GACGCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1457  GACGTCGGTC          
SmlI          597  CTCAAG               C^T[CT][GA]AG
              945  CTTGAG              
StyI          127  CCAAGG               C^C[AT][AT]GG
              212  CCTTGG              
TfiI          985  GATTC                G^A[AT]TC
             1041  GAATC               
Tsp45I       1086  GTCAC                ^GT[GC]AC
             1205  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BcnI        BfaI        BmyI        BpmI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrBI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TseI        TspRI       
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