Restriction map for pF1KA1853
 (Linear) (Six-base) MAPSORT of: KIAA1853.seq  Check: 1058  from: 1  to: 1833



KIAA1853.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:21  ..

AatII G_ACGT'C

Cuts at:      0    1461    1833 
   Size:       1461     372
 
AflIII A'CryG_T

Cuts at:      0     869    1833 
   Size:        869     964
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     139    1833 
   Size:        139    1694
 
AlwNI CAG_nnn'CTG

Cuts at:      0     198    1833 
   Size:        198    1635
 
AvaI C'yCGr_G

Cuts at:      0      82     911    1500    1833 
   Size:         82     829     589     333

  Fragments arranged by size: 

                 829     589     333      82
 
BamHI G'GATC_C

Cuts at:      0    1398    1833 
   Size:       1398     435
 
BanI G'GyrC_C

Cuts at:      0     217     259     614    1833 
   Size:        217      42     355    1219

  Fragments arranged by size: 

                1219     355     217      42
 
BanII G_rGCy'C

Cuts at:      0     140     212     583     934     965    1150    1330    1735 
   Size:        140      72     371     351      31     185     180     405

Cuts at:   1735    1833 
   Size:         98

  Fragments arranged by size: 

                 405     371     351     185     180     140      98      72
                  31
 
BbvCI CC'TCA_GC

Cuts at:      0    1588    1833 
   Size:       1588     245
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      78     112    1833 
   Size:         78      34    1721

  Fragments arranged by size: 

                1721      78      34
 
BlpI GC'TnA_GC

Cuts at:      0     900    1803    1833 
   Size:        900     903      30

  Fragments arranged by size: 

                 903     900      30
 
Bme1580I G_kGCm'C

Cuts at:      0     220    1536    1833 
   Size:        220    1316     297

  Fragments arranged by size: 

                1316     297     220
 
BmgBI CAC'GTC

Cuts at:      0     872    1833 
   Size:        872     961
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     265     702     719     785     929    1833 
   Size:        265     437      17      66     144     904

  Fragments arranged by size: 

                 904     437     265     144      66      17
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     873    1062    1437    1833 
   Size:        873     189     375     396

  Fragments arranged by size: 

                 873     396     375     189
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     300     721    1185    1531    1677    1803    1833 
   Size:        300     421     464     346     146     126      30

  Fragments arranged by size: 

                 464     421     346     300     146     126      30
 
Bpu10I CC'TnA_GC

Cuts at:      0     164    1588    1833 
   Size:        164    1424     245

  Fragments arranged by size: 

                1424     245     164
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     582     966    1833 
   Size:        582     384     867

  Fragments arranged by size: 

                 867     582     384
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     914    1833 
   Size:        914     919
 
BsaHI Gr'CG_yC

Cuts at:      0    1458    1833 
   Size:       1458     375
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     921     951    1644    1674    1801    1831    1833 
   Size:        921      30     693      30     127      30       2

  Fragments arranged by size: 

                 921     693     127      30      30      30       2
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     456     999    1167    1338    1563    1590    1833 
   Size:        456     543     168     171     225      27     243

  Fragments arranged by size: 

                 543     456     243     225     171     168      27
 
BseYI C'CCAG_C

Cuts at:      0     159     186    1297    1333    1384    1564    1833 
   Size:        159      27    1111      36      51     180     269

  Fragments arranged by size: 

                1111     269     180     159      51      36      27
 
BsiEI CG_ry'CG

Cuts at:      0     624    1833 
   Size:        624    1209
 
BsiHKAI G_wGCw'C

Cuts at:      0     583     965    1150    1833 
   Size:        583     382     185     683

  Fragments arranged by size: 

                 683     583     382     185
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1449    1671    1833 
   Size:       1449     222     162

  Fragments arranged by size: 

                1449     222     162
 
Bsp1286I G_dGCh'C

Cuts at:      0     140     212     220     583     934     965    1150    1330 
   Size:        140      72       8     363     351      31     185     180

Cuts at:   1330    1536    1735    1833 
   Size:        206     199      98

  Fragments arranged by size: 

                 363     351     206     199     185     180     140      98
                  72      31       8
 
BspHI T'CATG_A

Cuts at:      0     285    1833 
   Size:        285    1548
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     425    1833 
   Size:        425    1408
 
BsrBI CCG'CTC

Cuts at:      0     822    1292    1395    1628    1833 
   Size:        822     470     103     233     205

  Fragments arranged by size: 

                 822     470     233     205     103
 
BsrFI r'CCGG_y

Cuts at:      0     524     623    1833 
   Size:        524      99    1210

  Fragments arranged by size: 

                1210     524      99
 
BssSI C'ACGA_G

Cuts at:      0    1695    1824    1833 
   Size:       1695     129       9

  Fragments arranged by size: 

                1695     129       9
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     745    1833 
   Size:        745    1088
 
BstYI r'GATC_y

Cuts at:      0    1398    1833 
   Size:       1398     435
 
Bsu36I CC'TnA_GG

Cuts at:      0     632     701    1833 
   Size:        632      69    1132

  Fragments arranged by size: 

                1132     632      69
 
BtgI C'CryG_G

Cuts at:      0     618    1833 
   Size:        618    1215
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     368    1833 
   Size:        368    1465
 
EaeI y'GGCC_r

Cuts at:      0      68     200     621    1631    1833 
   Size:         68     132     421    1010     202

  Fragments arranged by size: 

                1010     421     202     132      68
 
EagI C'GGCC_G

Cuts at:      0     621    1833 
   Size:        621    1212
 
EarI CTCTTCn'nnn_

Cuts at:      0     349     445     483     493    1272    1833 
   Size:        349      96      38      10     779     561

  Fragments arranged by size: 

                 779     561     349      96      38      10
 
EcoICRI GAG'CTC

Cuts at:      0     581     963    1148    1833 
   Size:        581     382     185     685

  Fragments arranged by size: 

                 685     581     382     185
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     300     721    1185    1531    1677    1803    1833 
   Size:        300     421     464     346     146     126      30

  Fragments arranged by size: 

                 464     421     346     300     146     126      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     635     688    1833 
   Size:        635      53    1145

  Fragments arranged by size: 

                1145     635      53
 
EcoO109I rG'GnC_Cy

Cuts at:      0     365    1280    1349    1833 
   Size:        365     915      69     484

  Fragments arranged by size: 

                 915     484     365      69
 
HaeII r_GCGC'y

Cuts at:      0    1488    1833 
   Size:       1488     345
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     160     192     694     726     859     891    1644    1676 
   Size:        160      32     502      32     133      32     753      32

Cuts at:   1676    1801    1833 
   Size:        125      32

  Fragments arranged by size: 

                 753     502     160     133     125      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:      0     730    1833 
   Size:        730    1103
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1016    1432    1642    1833 
   Size:       1016     416     210     191

  Fragments arranged by size: 

                1016     416     210     191
 
MscI TGG'CCA

Cuts at:      0      70     202    1833 
   Size:         70     132    1631

  Fragments arranged by size: 

                1631     132      70
 
MspA1I CmG'CkG

Cuts at:      0     123     560     620     671    1431    1833 
   Size:        123     437      60      51     760     402

  Fragments arranged by size: 

                 760     437     402     123      60      51
 
NaeI GCC'GGC

Cuts at:      0     526    1833 
   Size:        526    1307
 
NgoMIV G'CCGG_C

Cuts at:      0     524    1833 
   Size:        524    1309
 
NruI TCG'CGA

Cuts at:      0     834    1833 
   Size:        834     999
 
PasI CC'CwG_GG

Cuts at:      0    1229    1833 
   Size:       1229     604
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     227    1833 
   Size:        227    1606
 
PfoI T'CCnGG_A

Cuts at:      0    1648    1833 
   Size:       1648     185
 
PpuMI rG'GwC_Cy

Cuts at:      0     365    1280    1349    1833 
   Size:        365     915      69     484

  Fragments arranged by size: 

                 915     484     365      69
 
PshAI GACnn'nnGTC

Cuts at:      0     435    1461    1833 
   Size:        435    1026     372

  Fragments arranged by size: 

                1026     435     372
 
SacI G_AGCT'C

Cuts at:      0     583     965    1150    1833 
   Size:        583     382     185     683

  Fragments arranged by size: 

                 683     583     382     185
 
SacII CC_GC'GG

Cuts at:      0     621    1833 
   Size:        621    1212
 
SfcI C'TryA_G

Cuts at:      0     375    1377    1797    1833 
   Size:        375    1002     420      36

  Fragments arranged by size: 

                1002     420     375      36
 
SmlI C'TyrA_G

Cuts at:      0     597     945    1833 
   Size:        597     348     888

  Fragments arranged by size: 

                 888     597     348
 
StyI C'CwwG_G

Cuts at:      0     127     212    1833 
   Size:        127      85    1621

  Fragments arranged by size: 

                1621     127      85
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     247    1167    1264    1451    1833 
   Size:        247     920      97     187     382

  Fragments arranged by size: 

                 920     382     247     187      97
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      63      95     921     953    1515    1547    1833 
   Size:         63      32     826      32     562      32     286

  Fragments arranged by size: 

                 826     562     286      63      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     210    1833 
   Size:        210    1623
 
XmnI GAAnn'nnTTC

Cuts at:      0     997    1833 
   Size:        997     836
 
ZraI GAC'GTC

Cuts at:      0    1459    1833 
   Size:       1459     374
 

 Enzymes that do cut:

    AatII   AflIII     AlfI    AlwNI     AvaI    BamHI     BanI    BanII
    BbvCI     BcgI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BssSI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     EaeI     EagI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII     MmeI
     MscI   MspA1I     NaeI   NgoMIV     NruI     PasI    PflMI     PfoI
    PpuMI    PshAI     SacI    SacII     SfcI     SmlI     StyI    TaqII
     TstI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI     BbeI     BbsI    BciVI     BclI    BfrBI
     BglI    BglII     BmtI    BsaAI    BsaBI    BsaWI     BsgI    BsiWI
     BsmI    BspEI    BsrDI    BsrGI   BssHII   BstAPI    BstBI   BstEII
  BstZ17I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EciI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MslI     NarI     NcoI     NdeI
     NheI     NotI     NsiI     NspI     PacI     PciI     PmeI     PmlI
     PpiI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI
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