Restriction map for pF1KA1826
 (Linear) (Six-base) MAPSORT of: KIAA1826.seq  Check: 8378  from: 1  to: 1035



KIAA1826.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     391    1035 
   Size:        391     644
 
Acc65I G'GTAC_C

Cuts at:      0     215    1035 
   Size:        215     820
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     733    1035 
   Size:        344     389     302

  Fragments arranged by size: 

                 389     344     302
 
ApoI r'AATT_y

Cuts at:      0     363     460    1010    1035 
   Size:        363      97     550      25

  Fragments arranged by size: 

                 550     363      97      25
 
AseI AT'TA_AT

Cuts at:      0     119    1035 
   Size:        119     916
 
BamHI G'GATC_C

Cuts at:      0     406     444    1035 
   Size:        406      38     591

  Fragments arranged by size: 

                 591     406      38
 
BanI G'GyrC_C

Cuts at:      0     215    1035 
   Size:        215     820
 
BglII A'GATC_T

Cuts at:      0     583    1035 
   Size:        583     452
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     919    1035 
   Size:        919     116
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     288     487     490    1035 
   Size:        288     199       3     545

  Fragments arranged by size: 

                 545     288     199       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     234    1035 
   Size:        234     801
 
BsmI GAATG_Cn'

Cuts at:      0     171    1035 
   Size:        171     864
 
Bsp1286I G_dGCh'C

Cuts at:      0     234    1035 
   Size:        234     801
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     391     669    1035 
   Size:        391     278     366

  Fragments arranged by size: 

                 391     366     278
 
BsrBI CCG'CTC

Cuts at:      0     744     765    1035 
   Size:        744      21     270

  Fragments arranged by size: 

                 744     270      21
 
BstBI TT'CG_AA

Cuts at:      0    1008    1035 
   Size:       1008      27
 
BstYI r'GATC_y

Cuts at:      0     406     444     583    1035 
   Size:        406      38     139     452

  Fragments arranged by size: 

                 452     406     139      38
 
DraIII CAC_nnn'GTG

Cuts at:      0     160    1035 
   Size:        160     875
 
EarI CTCTTCn'nnn_

Cuts at:      0     140     235     320     425    1035 
   Size:        140      95      85     105     610

  Fragments arranged by size: 

                 610     140     105      95      85
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     733    1035 
   Size:        344     389     302

  Fragments arranged by size: 

                 389     344     302
 
EcoRI G'AATT_C

Cuts at:      0    1010    1035 
   Size:       1010      25
 
EcoRV GAT'ATC

Cuts at:      0     660    1035 
   Size:        660     375
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     999    1021    1035 
   Size:        999      22      14

  Fragments arranged by size: 

                 999      22      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     644     676     805     837    1035 
   Size:        644      32     129      32     198

  Fragments arranged by size: 

                 644     198     129      32      32
 
KpnI G_GTAC'C

Cuts at:      0     219    1035 
   Size:        219     816
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     620    1035 
   Size:        620     415
 
MslI CAynn'nnrTG

Cuts at:      0     548    1035 
   Size:        548     487
 
NdeI CA'TA_TG

Cuts at:      0     591    1035 
   Size:        591     444
 
PfoI T'CCnGG_A

Cuts at:      0     511    1035 
   Size:        511     524
 
PstI C_TGCA'G

Cuts at:      0     680     773     938     968    1035 
   Size:        680      93     165      30      67

  Fragments arranged by size: 

                 680     165      93      67      30
 
SfcI C'TryA_G

Cuts at:      0     170     676     769     934     964    1035 
   Size:        170     506      93     165      30      71

  Fragments arranged by size: 

                 506     170     165      93      71      30
 
SmlI C'TyrA_G

Cuts at:      0     898    1035 
   Size:        898     137
 
SspI AAT'ATT

Cuts at:      0     618    1035 
   Size:        618     417
 
StyI C'CwwG_G

Cuts at:      0     824    1035 
   Size:        824     211
 
XbaI T'CTAG_A

Cuts at:      0     580    1035 
   Size:        580     455
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     267    1035 
   Size:        267     768
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     ApoI     AseI    BamHI     BanI    BglII
    BpuEI    BseRI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BstBI
    BstYI   DraIII     EarI  Eco57MI    EcoRI    EcoRV     FalI    Hin4I
     KpnI     MmeI     MslI     NdeI     PfoI     PstI     SfcI     SmlI
     SspI     StyI     XbaI     XcmI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com