Restriction map for pF1KA1803
##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AhdI        AocI        Aor51HI     AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BamHI       BfrI        BglI        BlnI        BsaBI       
BseAI       BsiWI       Bsp1407I    BspDI       BspEI       
BsrGI       BssHII      Bsu36I      ClaI        CpoI        
DrdI        Eam1105I    Eco47III    Eco81I      EcoNI       
EcoRV       FseI        FspI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NcoI        NotI        NruI        PacI        PflMI       
PmeI        PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SfiI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        TfiI        Van91I      XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccII        2779  CGCG                 CG^CG
AflIII       4011  ACATGT               A^C[GA][CT]GT
AgeI         1265  ACCGGT               A^CCGGT
Asp700I      3369  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         2707  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         2765  TGGCCA               TGG^CCA
BbrPI        3430  CACGTG               CAC^GTG
BciVI        2686  GGATAC               GTATCC(6/5)
BclI         1763  TGATCA               T^GATCA
BglII        3474  AGATCT               A^GATCT
BlpI         1800  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1800  GCTGAGC              GC^T[ACGT]AGC
BsaAI        3430  CACGTG               [CT]AC^GT[GA]
BsgI         3541  CTGCAC               GTGCAG(16/14)
BsmBI        1228  GAGACG               CGTCTC(1/5)
BspHI        2742  TCATGA               T^CATGA
BspLU11I     4011  ACATGT               A^CATGT
BsrBI         301  GAGCGG               CCGCTC(-3/-3)
BssSI        1545  CACGAG               CACGAG(-5/-1)
Bst1107I      830  GTATAC               GTA^TAC
BstEII       1161  GGTGACC              G^GT[ACGT]ACC
BstPI        1161  GGTGACC              G^GT[ACGT]ACC
BstUI        2779  CGCG                 CG^CG
BstZ17I       830  GTATAC               GTA^TAC
CelII        1800  GCTGAGC              GC^T[ACGT]AGC
DraI         3409  TTTAAA               TTT^AAA
EcoO65I      1161  GGTGACC              G^GT[ACGT]ACC
EcoT22I      2125  ATGCAT               ATGCA^T
FbaI         1763  TGATCA               T^GATCA
HincII       3805  GTTAAC               GT[CT]^[GA]AC
HindII       3805  GTTAAC               GT[CT]^[GA]AC
HindIII      3370  AAGCTT               A^AGCTT
HpaI         3805  GTTAAC               GTT^AAC
MluNI        2765  TGGCCA               TGG^CCA
MscI         2765  TGGCCA               TGG^CCA
MvnI         2779  CGCG                 CG^CG
NaeI         2918  GCCGGC               GCC^GGC
NdeI         2845  CATATG               CA^TATG
NgoMIV       2918  GCCGGC               G^CCGGC
NheI          906  GCTAGC               G^CTAGC
NsiI         2125  ATGCAT               ATGCA^T
PaeR7I       1390  CTCGAG               C^TCGAG
PflFI        2707  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1265  ACCGGT               A^CCGGT
PmaCI        3430  CACGTG               CAC^GTG
PmlI         3430  CACGTG               CAC^GTG
PpuMI        2150  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI        1145  GACACCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         2742  TCATGA               T^CATGA
SacI         3846  GAGCTC               GAGCT^C
SapI         3550  GAAGAGC              GCTCTTC(1/4)
ScaI         1532  AGTACT               AGT^ACT
SexAI        1493  ACCTGGT              A^CC[AT]GGT
StuI         2380  AGGCCT               AGG^CCT
Tth111I      2707  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          444  TCTAGA               T^CTAGA
XcmI         2606  CCAGTGGCGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1390  CTCGAG               C^TCGAG
XmnI         3369  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI          830  GTATAC               GT^[AC][GT]AC
             1075  GTCTAC              
ApaI           19  GGGCCC               GGGCC^C
             3465  GGGCCC              
BbeI         1258  GGCGCC               GGCGC^C
             2611  GGCGCC              
BbsI         3133  GAAGAC               GAAGAC(2/6)
             3898  GAAGAC              
BbvCI        1795  CCTCAGC              CCTCAGC(-5/-2)
             1168  GCTGAGG             
BfaI          445  CTAG                 C^TAG
              907  CTAG                
BpuAI        3133  GAAGAC               GAAGAC(2/6)
             3898  GAAGAC              
BsaI         3724  GAGACC               GGTCTC(1/5)
             1497  GGTCTC              
BsmI         4215  GAATGC               GAATGC(1/-1)
              703  GCATTC              
BspMI        3640  ACCTGC               ACCTGC(4/8)
             4050  GCAGGT              
BstBI         233  TTCGAA               TT^CGAA
              661  TTCGAA              
BstXI        1934  CCAGCAAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1988  CCACACTGATGG        
BstYI        1238  GGATCT               [GA]^GATC[CT]
             3474  AGATCT              
DraIII       1504  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3532  CACATCGTG           
EagI         1958  CGGCCG               C^GGCCG
             2677  CGGCCG              
EclXI        1958  CGGCCG               C^GGCCG
             2677  CGGCCG              
Eco52I       1958  CGGCCG               C^GGCCG
             2677  CGGCCG              
EcoT14I        24  CCAAGG               C^C[AT][AT]GG
             1781  CCAAGG              
HgaI         2602  GACGC                GACGC(5/10)
             3339  GCGTC               
KasI         1258  GGCGCC               G^GCGCC
             2611  GGCGCC              
MaeI          445  CTAG                 C^TAG
              907  CTAG                
MflI         1238  GGATCT               [GA]^GATC[CT]
             3474  AGATCT              
NarI         1258  GGCGCC               GG^CGCC
             2611  GGCGCC              
NspV          233  TTCGAA               TT^CGAA
              661  TTCGAA              
PstI          901  CTGCAG               CTGCA^G
             2108  CTGCAG              
SfoI         1258  GGCGCC               GGC^GCC
             2611  GGCGCC              
SfuI          233  TTCGAA               TT^CGAA
              661  TTCGAA              
SmlI         1390  CTCGAG               C^T[CT][GA]AG
             3025  CTCAAG              
SphI         2123  GCATGC               GCATG^C
             2958  GCATGC              
StyI           24  CCAAGG               C^C[AT][AT]GG
             1781  CCAAGG              
XhoII        1238  GGATCT               [GA]^GATC[CT]
             3474  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       ApaLI       ApoI        
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BcgI        BcnI        BmyI        BpmI        
BsaHI       BsaJI       BsaWI       BseRI       BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrDI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRI       EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
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