Restriction map for pF1KA1803
 (Linear) (Six-base) MAPSORT of: KIAA1803.seq  Check: 7120  from: 1  to: 4236



KIAA1803.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3649    4041    4236 
   Size:       3649     392     195

  Fragments arranged by size: 

                3649     392     195
 
AccI GT'mk_AC

Cuts at:      0     831    1076    4236 
   Size:        831     245    3160

  Fragments arranged by size: 

                3160     831     245
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     896    1430    3031    3143    3299    3917    4236 
   Size:        896     534    1601     112     156     618     319

  Fragments arranged by size: 

                1601     896     618     534     319     156     112
 
AflIII A'CryG_T

Cuts at:      0    4011    4236 
   Size:       4011     225
 
AgeI A'CCGG_T

Cuts at:      0    1265    4236 
   Size:       1265    2971
 
AleI CACnn'nnGTG

Cuts at:      0    1411    4236 
   Size:       1411    2825
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2360    4236 
   Size:       2360    1876
 
AlwNI CAG_nnn'CTG

Cuts at:      0     653     811    2539    2733    4236 
   Size:        653     158    1728     194    1503

  Fragments arranged by size: 

                1728    1503     653     194     158
 
ApaI G_GGCC'C

Cuts at:      0      23    3469    4236 
   Size:         23    3446     767

  Fragments arranged by size: 

                3446     767      23
 
ApaLI G'TGCA_C

Cuts at:      0     948    1180    2461    4236 
   Size:        948     232    1281    1775

  Fragments arranged by size: 

                1775    1281     948     232
 
ApoI r'AATT_y

Cuts at:      0    1345    2284    2446    3298    3307    3967    4236 
   Size:       1345     939     162     852       9     660     269

  Fragments arranged by size: 

                1345     939     852     660     269     162       9
 
AvaI C'yCGr_G

Cuts at:      0    1390    1396    3457    4236 
   Size:       1390       6    2061     779

  Fragments arranged by size: 

                2061    1390     779       6
 
BanI G'GyrC_C

Cuts at:      0    1069    1258    1517    2403    2611    4236 
   Size:       1069     189     259     886     208    1625

  Fragments arranged by size: 

                1625    1069     886     259     208     189
 
BanII G_rGCy'C

Cuts at:      0      23     506    1397    1420    1463    1599    3164    3469 
   Size:         23     483     891      23      43     136    1565     305

Cuts at:   3469    3850    3997    4236 
   Size:        381     147     239

  Fragments arranged by size: 

                1565     891     483     381     305     239     147     136
                  43      23      23
 
BbeI G_GCGC'C

Cuts at:      0    1262    2615    4236 
   Size:       1262    1353    1621

  Fragments arranged by size: 

                1621    1353    1262
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    3140    3905    4236 
   Size:       3140     765     331

  Fragments arranged by size: 

                3140     765     331
 
BbvCI CC'TCA_GC

Cuts at:      0    1169    1796    4236 
   Size:       1169     627    2440

  Fragments arranged by size: 

                2440    1169     627
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     224     258    1684    1718    2465    2499    4236 
   Size:        224      34    1426      34     747      34    1737

  Fragments arranged by size: 

                1737    1426     747     224      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2680    4236 
   Size:       2680    1556
 
BclI T'GATC_A

Cuts at:      0    1763    4236 
   Size:       1763    2473
 
BfrBI ATG'CAT

Cuts at:      0    2127    4236 
   Size:       2127    2109
 
BglII A'GATC_T

Cuts at:      0    3474    4236 
   Size:       3474     762
 
BlpI GC'TnA_GC

Cuts at:      0    1801    4236 
   Size:       1801    2435
 
Bme1580I G_kGCm'C

Cuts at:      0      23     952    1184    1635    2465    3446    3469    4236 
   Size:         23     929     232     451     830     981      23     767

  Fragments arranged by size: 

                 981     929     830     767     451     232      23      23
            
 
BmgBI CAC'GTC

Cuts at:      0     422    1221    2505    4236 
   Size:        422     799    1284    1731

  Fragments arranged by size: 

                1731    1284     799     422
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1356    4236 
   Size:       1356    2880
 
BmtI G_CTAG'C

Cuts at:      0     910    4236 
   Size:        910    3326
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2274    4236 
   Size:       2274    1962
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319    1716    3630    3646    3834    4231    4236 
   Size:        319    1397    1914      16     188     397       5

  Fragments arranged by size: 

                1914    1397     397     319     188      16       5
 
Bpu10I CC'TnA_GC

Cuts at:      0    1169    1796    2048    4236 
   Size:       1169     627     252    2188

  Fragments arranged by size: 

                2188    1169     627     252
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3010    4236 
   Size:       3010    1226
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1503    3718    4236 
   Size:       1503    2215     518

  Fragments arranged by size: 

                2215    1503     518
 
BsaAI yAC'GTr

Cuts at:      0    3432    4236 
   Size:       3432     804
 
BsaHI Gr'CG_yC

Cuts at:      0    1259    2603    2612    3339    4236 
   Size:       1259    1344       9     727     897

  Fragments arranged by size: 

                1344    1259     897     727       9
 
BsaWI w'CCGG_w

Cuts at:      0     140    1265    1514    4236 
   Size:        140    1125     249    2722

  Fragments arranged by size: 

                2722    1125     249     140
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1450    1480    1483    1513    1525    1555    3085    3115 
   Size:       1450      30       3      30      12      30    1530      30

Cuts at:   3115    4236 
   Size:       1121

  Fragments arranged by size: 

                1530    1450    1121      30      30      30      30      12
                   3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1240    1456    1459    1471    2020    2328    2763    3067 
   Size:       1240     216       3      12     549     308     435     304

Cuts at:   3067    3072    3090    3303    3475    4236 
   Size:          5      18     213     172     761

  Fragments arranged by size: 

                1240     761     549     435     308     304     216     213
                 172      18      12       5       3
 
BseYI C'CCAG_C

Cuts at:      0     586    1598    2523    3439    4236 
   Size:        586    1012     925     916     797

  Fragments arranged by size: 

                1012     925     916     797     586
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3526    4236 
   Size:       3526     710
 
BsiEI CG_ry'CG

Cuts at:      0     249    1961    2680    4236 
   Size:        249    1712     719    1556

  Fragments arranged by size: 

                1712    1556     719     249
 
BsiHKAI G_wGCw'C

Cuts at:      0     327     346     952    1184    1843    1852    2465    2983 
   Size:        327      19     606     232     659       9     613     518

Cuts at:   2983    3850    4236 
   Size:        867     386

  Fragments arranged by size: 

                 867     659     613     606     518     386     327     232
                  19       9
 
BsmI GAATG_Cn'

Cuts at:      0     703    4221    4236 
   Size:        703    3518      15

  Fragments arranged by size: 

                3518     703      15
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1222    4236 
   Size:       1222    3014
 
Bsp1286I G_dGCh'C

Cuts at:      0      23     327     346     506     952    1184    1397    1420 
   Size:         23     304      19     160     446     232     213      23

Cuts at:   1420    1463    1599    1635    1843    1852    2465    2983    3164 
   Size:         43     136      36     208       9     613     518     181

Cuts at:   3164    3446    3469    3850    3997    4236 
   Size:        282      23     381     147     239

  Fragments arranged by size: 

                 613     518     446     381     304     282     239     232
                 213     208     181     160     147     136      43      36
                  23      23      23      19       9
 
BspHI T'CATG_A

Cuts at:      0    2742    4236 
   Size:       2742    1494
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3649    4041    4236 
   Size:       3649     392     195

  Fragments arranged by size: 

                3649     392     195
 
BsrBI CCG'CTC

Cuts at:      0     303    4236 
   Size:        303    3933
 
BsrDI GCAATG_nn'

Cuts at:      0    1089    2523    2691    4236 
   Size:       1089    1434     168    1545

  Fragments arranged by size: 

                1545    1434    1089     168
 
BsrFI r'CCGG_y

Cuts at:      0    1265    2918    3578    4236 
   Size:       1265    1653     660     658

  Fragments arranged by size: 

                1653    1265     660     658
 
BssSI C'ACGA_G

Cuts at:      0    1545    4236 
   Size:       1545    2691
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     956    1091    2084    2353    2733    2891    3389    4236 
   Size:        956     135     993     269     380     158     498     847

  Fragments arranged by size: 

                 993     956     847     498     380     269     158     135
            
 
BstBI TT'CG_AA

Cuts at:      0     234     662    4236 
   Size:        234     428    3574

  Fragments arranged by size: 

                3574     428     234
 
BstEII G'GTnAC_C

Cuts at:      0    1161    4236 
   Size:       1161    3075
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1941    1995    4236 
   Size:       1941      54    2241

  Fragments arranged by size: 

                2241    1941      54
 
BstYI r'GATC_y

Cuts at:      0    1238    3474    4236 
   Size:       1238    2236     762

  Fragments arranged by size: 

                2236    1238     762
 
BstZ17I GTA'TAC

Cuts at:      0     832    4236 
   Size:        832    3404
 
BtgI C'CryG_G

Cuts at:      0    1116    2859    3033    3061    3927    4236 
   Size:       1116    1743     174      28     866     309

  Fragments arranged by size: 

                1743    1116     866     309     174      28
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     447    3872    4236 
   Size:        447    3425     364

  Fragments arranged by size: 

                3425     447     364
 
BtsI GCAGTG_nn'

Cuts at:      0     346     471    1716    1829    2105    2640    3291    4236 
   Size:        346     125    1245     113     276     535     651     945

  Fragments arranged by size: 

                1245     945     651     535     346     276     125     113
            
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3342    3377    4236 
   Size:       3342      35     859

  Fragments arranged by size: 

                3342     859      35
 
DraI TTT'AAA

Cuts at:      0    3411    4236 
   Size:       3411     825
 
DraIII CAC_nnn'GTG

Cuts at:      0    1509    3537    4236 
   Size:       1509    2028     699

  Fragments arranged by size: 

                2028    1509     699
 
EaeI y'GGCC_r

Cuts at:      0      37     224    1958    2677    2765    4236 
   Size:         37     187    1734     719      88    1471

  Fragments arranged by size: 

                1734    1471     719     187      88      37
 
EagI C'GGCC_G

Cuts at:      0    1958    2677    4236 
   Size:       1958     719    1559

  Fragments arranged by size: 

                1958    1559     719
 
EarI CTCTTCn'nnn_

Cuts at:      0     791    1627    1997    2393    3545    4236 
   Size:        791     836     370     396    1152     691

  Fragments arranged by size: 

                1152     836     791     691     396     370
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2569    4236 
   Size:       2569    1667
 
EcoICRI GAG'CTC

Cuts at:      0    3848    4236 
   Size:       3848     388
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     896    1430    1716    3031    3143    3299    3630 
   Size:        319     577     534     286    1315     112     156     331

Cuts at:   3630    3646    3834    3917    4231    4236 
   Size:         16     188      83     314       5

  Fragments arranged by size: 

                1315     577     534     331     319     314     286     188
                 156     112      83      16       5
 
EcoO109I rG'GnC_Cy

Cuts at:      0      19      20     808    2151    3465    3466    4236 
   Size:         19       1     788    1343    1314       1     770

  Fragments arranged by size: 

                1343    1314     788     770      19       1       1
 
EcoRI G'AATT_C

Cuts at:      0    2284    3298    3307    4236 
   Size:       2284    1014       9     929

  Fragments arranged by size: 

                2284    1014     929       9
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1860    2115    2181    3304    3651    4236 
   Size:       1860     255      66    1123     347     585

  Fragments arranged by size: 

                1860    1123     585     347     255      66
 
HaeII r_GCGC'y

Cuts at:      0    1262    1692    2615    4236 
   Size:       1262     430     923    1621

  Fragments arranged by size: 

                1621    1262     923     430
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1450    1482    2873    2905    3057    3089    4052    4084 
   Size:       1450      32    1391      32     152      32     963      32

Cuts at:   4084    4236 
   Size:        152

  Fragments arranged by size: 

                1450    1391     963     152     152      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    3807    4236 
   Size:       3807     429
 
HindIII A'AGCT_T

Cuts at:      0    3370    4236 
   Size:       3370     866
 
HpaI GTT'AAC

Cuts at:      0    3807    4236 
   Size:       3807     429
 
KasI G'GCGC_C

Cuts at:      0    1258    2611    4236 
   Size:       1258    1353    1625

  Fragments arranged by size: 

                1625    1353    1258
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2642    3777    4236 
   Size:       2642    1135     459

  Fragments arranged by size: 

                2642    1135     459
 
MscI TGG'CCA

Cuts at:      0    2767    4236 
   Size:       2767    1469
 
MslI CAynn'nnrTG

Cuts at:      0     330     565     945    1024    1159    1411    1586    1993 
   Size:        330     235     380      79     135     252     175     407

Cuts at:   1993    2880    2951    4236 
   Size:        887      71    1285

  Fragments arranged by size: 

                1285     887     407     380     330     252     235     175
                 135      79      71
 
MspA1I CmG'CkG

Cuts at:      0      60     272    1168    1800    2075    2527    2536    2795 
   Size:         60     212     896     632     275     452       9     259

Cuts at:   2795    3009    3202    3620    3819    4236 
   Size:        214     193     418     199     417

  Fragments arranged by size: 

                 896     632     452     418     417     275     259     214
                 212     199     193      60       9
 
NaeI GCC'GGC

Cuts at:      0    2920    4236 
   Size:       2920    1316
 
NarI GG'CG_CC

Cuts at:      0    1259    2612    4236 
   Size:       1259    1353    1624

  Fragments arranged by size: 

                1624    1353    1259
 
NdeI CA'TA_TG

Cuts at:      0    2846    4236 
   Size:       2846    1390
 
NgoMIV G'CCGG_C

Cuts at:      0    2918    4236 
   Size:       2918    1318
 
NheI G'CTAG_C

Cuts at:      0     906    4236 
   Size:        906    3330
 
NsiI A_TGCA'T

Cuts at:      0    2129    4236 
   Size:       2129    2107
 
NspI r_CATG'y

Cuts at:      0    2127    2962    4015    4236 
   Size:       2127     835    1053     221

  Fragments arranged by size: 

                2127    1053     835     221
 
PasI CC'CwG_GG

Cuts at:      0     713    1906    4236 
   Size:        713    1193    2330

  Fragments arranged by size: 

                2330    1193     713
 
PciI A'CATG_T

Cuts at:      0    4011    4236 
   Size:       4011     225
 
PfoI T'CCnGG_A

Cuts at:      0     845    1732    2576    4208    4236 
   Size:        845     887     844    1632      28

  Fragments arranged by size: 

                1632     887     845     844      28
 
PmlI CAC'GTG

Cuts at:      0    3432    4236 
   Size:       3432     804
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3008    3040    4236 
   Size:       3008      32    1196

  Fragments arranged by size: 

                3008    1196      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2151    4236 
   Size:       2151    2085
 
PshAI GACnn'nnGTC

Cuts at:      0    1149    4236 
   Size:       1149    3087
 
PspOMI G'GGCC_C

Cuts at:      0      19    3465    4236 
   Size:         19    3446     771

  Fragments arranged by size: 

                3446     771      19
 
PspXI vC'TCGA_Gb

Cuts at:      0    1390    4236 
   Size:       1390    2846
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2191    2223    4236 
   Size:       2191      32    2013

  Fragments arranged by size: 

                2191    2013      32
 
PstI C_TGCA'G

Cuts at:      0     905    2112    4236 
   Size:        905    1207    2124

  Fragments arranged by size: 

                2124    1207     905
 
PvuII CAG'CTG

Cuts at:      0      60     272    1800    2527    2536    2795    3009    3202 
   Size:         60     212    1528     727       9     259     214     193

Cuts at:   3202    3819    4236 
   Size:        617     417

  Fragments arranged by size: 

                1528     727     617     417     259     214     212     193
                  60       9
 
SacI G_AGCT'C

Cuts at:      0    3850    4236 
   Size:       3850     386
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3545    4236 
   Size:       3545     691
 
ScaI AGT'ACT

Cuts at:      0    1534    4236 
   Size:       1534    2702
 
SexAI A'CCwGG_T

Cuts at:      0    1493    4236 
   Size:       1493    2743
 
SfcI C'TryA_G

Cuts at:      0     639     901    2108    4236 
   Size:        639     262    1207    2128

  Fragments arranged by size: 

                2128    1207     639     262
 
SfoI GGC'GCC

Cuts at:      0    1260    2613    4236 
   Size:       1260    1353    1623

  Fragments arranged by size: 

                1623    1353    1260
 
SmlI C'TyrA_G

Cuts at:      0    1390    3025    4236 
   Size:       1390    1635    1211

  Fragments arranged by size: 

                1635    1390    1211
 
SphI G_CATG'C

Cuts at:      0    2127    2962    4236 
   Size:       2127     835    1274

  Fragments arranged by size: 

                2127    1274     835
 
StuI AGG'CCT

Cuts at:      0    2382    4236 
   Size:       2382    1854
 
StyI C'CwwG_G

Cuts at:      0      24    1781    4236 
   Size:         24    1757    2455

  Fragments arranged by size: 

                2455    1757      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     263    1919    2480    4088    4236 
   Size:        263    1656     561    1608     148

  Fragments arranged by size: 

                1656    1608     561     263     148
 
TatI w'GTAC_w

Cuts at:      0    1532    2789    4236 
   Size:       1532    1257    1447

  Fragments arranged by size: 

                1532    1447    1257
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1483    1515    2044    2076    3085    3117    4236 
   Size:       1483      32     529      32    1009      32    1119

  Fragments arranged by size: 

                1483    1119    1009     529      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2710    4236 
   Size:       2710    1526
 
XbaI T'CTAG_A

Cuts at:      0     444    4236 
   Size:        444    3792
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2613    4236 
   Size:       2613    1623
 
XhoI C'TCGA_G

Cuts at:      0    1390    4236 
   Size:       1390    2846
 
XmnI GAAnn'nnTTC

Cuts at:      0    3373    4236 
   Size:       3373     863
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AgeI     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NsiI     NspI
     PasI     PciI     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI    PvuII     SacI     SapI     ScaI    SexAI
     SfcI     SfoI     SmlI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AhdI     AloI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BglI    BsaBI    BsiWI
    BspEI    BsrGI   BssHII   Bsu36I     ClaI     DrdI    EcoNI    EcoRV
     FseI     FspI    FspAI     KpnI     MfeI     MluI     NcoI     NotI
     NruI     PacI    PflMI     PmeI     PsiI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     SwaI     XmaI     ZraI
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