Restriction map for
pF1KA1757
(Linear) (Six-base) MAPSORT of: KIAA1757.seq Check: 5422 from: 1 to: 3993
KIAA1757.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AatII G_ACGT'C
Cuts at: 0 1968 3993
Size: 1968 2025
AccI GT'mk_AC
Cuts at: 0 115 1112 3863 3993
Size: 115 997 2751 130
Fragments arranged by size:
2751 997 130 115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 1022 1367 1382 1667 3415 3435 3518
Size: 266 756 345 15 285 1748 20 83
Cuts at: 3518 3993
Size: 475
Fragments arranged by size:
1748 756 475 345 285 266 83 20
15
AfeI AGC'GCT
Cuts at: 0 2014 3993
Size: 2014 1979
AflIII A'CryG_T
Cuts at: 0 1877 3993
Size: 1877 2116
AhdI GACnn_n'nnGTC
Cuts at: 0 3833 3993
Size: 3833 160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1592 3993
Size: 1592 2401
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 923 955 3993
Size: 923 32 3038
Fragments arranged by size:
3038 923 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1696 2003 2962 3301 3390 3954 3993
Size: 1696 307 959 339 89 564 39
Fragments arranged by size:
1696 959 564 339 307 89 39
ApaLI G'TGCA_C
Cuts at: 0 3931 3993
Size: 3931 62
ApoI r'AATT_y
Cuts at: 0 234 255 391 655 1520 2907 3052 3993
Size: 234 21 136 264 865 1387 145 941
Fragments arranged by size:
1387 941 865 264 234 145 136 21
AseI AT'TA_AT
Cuts at: 0 425 3993
Size: 425 3568
AvaI C'yCGr_G
Cuts at: 0 2648 3993
Size: 2648 1345
BamHI G'GATC_C
Cuts at: 0 1723 1848 2785 3016 3993
Size: 1723 125 937 231 977
Fragments arranged by size:
1723 977 937 231 125
BanI G'GyrC_C
Cuts at: 0 2998 3993
Size: 2998 995
BanII G_rGCy'C
Cuts at: 0 2155 3257 3993
Size: 2155 1102 736
Fragments arranged by size:
2155 1102 736
BbsI GAAGACnn'nnnn_
Cuts at: 0 1427 3993
Size: 1427 2566
BbvCI CC'TCA_GC
Cuts at: 0 815 3021 3993
Size: 815 2206 972
Fragments arranged by size:
2206 972 815
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2980 3014 3993
Size: 2980 34 979
Fragments arranged by size:
2980 979 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1101 2311 2422 2874 3472 3993
Size: 1101 1210 111 452 598 521
Fragments arranged by size:
1210 1101 598 521 452 111
BclI T'GATC_A
Cuts at: 0 1098 3993
Size: 1098 2895
BfrBI ATG'CAT
Cuts at: 0 1224 3993
Size: 1224 2769
BglI GCCn_nnn'nGGC
Cuts at: 0 1500 3993
Size: 1500 2493
BlpI GC'TnA_GC
Cuts at: 0 3333 3993
Size: 3333 660
Bme1580I G_kGCm'C
Cuts at: 0 1819 1831 3003 3935 3993
Size: 1819 12 1172 932 58
Fragments arranged by size:
1819 1172 932 58 12
BmgBI CAC'GTC
Cuts at: 0 3557 3993
Size: 3557 436
BmrI ACTGGGnnnn_n'
Cuts at: 0 3836 3993
Size: 3836 157
BmtI G_CTAG'C
Cuts at: 0 3704 3993
Size: 3704 289
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3309 3993
Size: 3309 684
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1469 2207 2893 3458 3858 3931 3993
Size: 1469 738 686 565 400 73 62
Fragments arranged by size:
1469 738 686 565 400 73 62
Bpu10I CC'TnA_GC
Cuts at: 0 815 2900 3021 3993
Size: 815 2085 121 972
Fragments arranged by size:
2085 972 815 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3125 3993
Size: 3125 868
BsaI GGTCTCn'nnnn_
Cuts at: 0 3498 3993
Size: 3498 495
BsaAI yAC'GTr
Cuts at: 0 1837 3993
Size: 1837 2156
BsaBI GATnn'nnATC
Cuts at: 0 2561 3993
Size: 2561 1432
BsaHI Gr'CG_yC
Cuts at: 0 1965 3993
Size: 1965 2028
BsaWI w'CCGG_w
Cuts at: 0 512 890 3993
Size: 512 378 3103
Fragments arranged by size:
3103 512 378
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 923 953 2422 2452 3341 3371 3410 3440
Size: 923 30 1469 30 889 30 39 30
Cuts at: 3440 3456 3486 3856 3886 3912 3942 3993
Size: 16 30 370 30 26 30 51
Fragments arranged by size:
1469 923 889 370 51 39 30 30
30 30 30 30 30 26 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 941 1057 2317 2366 2492 2666 3693
Size: 103 838 116 1260 49 126 174 1027
Cuts at: 3693 3993
Size: 300
Fragments arranged by size:
1260 1027 838 300 174 126 116 103
49
BseYI C'CCAG_C
Cuts at: 0 80 281 483 2264 2962 3847 3993
Size: 80 201 202 1781 698 885 146
Fragments arranged by size:
1781 885 698 202 201 146 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 365 2918 3031 3482 3993
Size: 227 138 2553 113 451 511
Fragments arranged by size:
2553 511 451 227 138 113
BsiEI CG_ry'CG
Cuts at: 0 1510 3993
Size: 1510 2483
BsiHKAI G_wGCw'C
Cuts at: 0 644 3935 3993
Size: 644 3291 58
Fragments arranged by size:
3291 644 58
BsmI GAATG_Cn'
Cuts at: 0 2643 3993
Size: 2643 1350
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1956 3993
Size: 1956 2037
Bsp1286I G_dGCh'C
Cuts at: 0 644 1819 1831 2155 3003 3257 3935 3993
Size: 644 1175 12 324 848 254 678 58
Fragments arranged by size:
1175 848 678 644 324 254 58 12
BspHI T'CATG_A
Cuts at: 0 1101 3993
Size: 1101 2892
BsrDI GCAATG_nn'
Cuts at: 0 2106 3808 3993
Size: 2106 1702 185
Fragments arranged by size:
2106 1702 185
BsrGI T'GTAC_A
Cuts at: 0 351 727 3993
Size: 351 376 3266
Fragments arranged by size:
3266 376 351
BssSI C'ACGA_G
Cuts at: 0 497 2572 2780 3993
Size: 497 2075 208 1213
Fragments arranged by size:
2075 1213 497 208
BstEII G'GTnAC_C
Cuts at: 0 819 3993
Size: 819 3174
BstYI r'GATC_y
Cuts at: 0 518 1723 1848 2671 2785 3016 3993
Size: 518 1205 125 823 114 231 977
Fragments arranged by size:
1205 977 823 518 231 125 114
BstZ17I GTA'TAC
Cuts at: 0 116 3993
Size: 116 3877
Bsu36I CC'TnA_GG
Cuts at: 0 523 3993
Size: 523 3470
BtgI C'CryG_G
Cuts at: 0 3003 3915 3993
Size: 3003 912 78
Fragments arranged by size:
3003 912 78
BtsI GCAGTG_nn'
Cuts at: 0 72 3129 3400 3952 3993
Size: 72 3057 271 552 41
Fragments arranged by size:
3057 552 271 72 41
DraIII CAC_nnn'GTG
Cuts at: 0 1411 2800 3582 3916 3993
Size: 1411 1389 782 334 77
Fragments arranged by size:
1411 1389 782 334 77
DrdI GACnn_nn'nnGTC
Cuts at: 0 1559 3993
Size: 1559 2434
EaeI y'GGCC_r
Cuts at: 0 1507 2270 3993
Size: 1507 763 1723
Fragments arranged by size:
1723 1507 763
EagI C'GGCC_G
Cuts at: 0 1507 3993
Size: 1507 2486
EarI CTCTTCn'nnn_
Cuts at: 0 483 649 1133 1142 1328 1507 1607 2121
Size: 483 166 484 9 186 179 100 514
Cuts at: 2121 2294 3555 3993
Size: 173 1261 438
Fragments arranged by size:
1261 514 484 483 438 186 179 173
166 100 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 48 3993
Size: 48 3945
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 1022 1367 1382 1469 1667 2207 2893
Size: 266 756 345 15 87 198 540 686
Cuts at: 2893 3415 3435 3458 3518 3858 3931 3993
Size: 522 20 23 60 340 73 62
Fragments arranged by size:
756 686 540 522 345 340 266 198
87 73 62 60 23 20 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2732 3993
Size: 2732 1261
EcoO109I rG'GnC_Cy
Cuts at: 0 1502 1973 2810 3216 3718 3993
Size: 1502 471 837 406 502 275
Fragments arranged by size:
1502 837 502 471 406 275
EcoRI G'AATT_C
Cuts at: 0 234 3993
Size: 234 3759
HaeII r_GCGC'y
Cuts at: 0 2016 3993
Size: 2016 1977
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 1045 1077 1715 1747 2064 2096
Size: 34 32 979 32 638 32 317 32
Cuts at: 2096 2309 2341 3191 3223 3819 3851 3993
Size: 213 32 850 32 596 32 142
Fragments arranged by size:
979 850 638 596 317 213 142 34
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1734 3993
Size: 1734 2259
HindIII A'AGCT_T
Cuts at: 0 323 3993
Size: 323 3670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1876 2465 3014 3993
Size: 1876 589 549 979
Fragments arranged by size:
1876 979 589 549
MscI TGG'CCA
Cuts at: 0 2272 3993
Size: 2272 1721
MslI CAynn'nnrTG
Cuts at: 0 2708 3993
Size: 2708 1285
MspA1I CmG'CkG
Cuts at: 0 483 2268 2453 3859 3993
Size: 483 1785 185 1406 134
Fragments arranged by size:
1785 1406 483 185 134
NcoI C'CATG_G
Cuts at: 0 3003 3993
Size: 3003 990
NheI G'CTAG_C
Cuts at: 0 3700 3993
Size: 3700 293
NsiI A_TGCA'T
Cuts at: 0 1226 3993
Size: 1226 2767
NspI r_CATG'y
Cuts at: 0 1228 1881 3993
Size: 1228 653 2112
Fragments arranged by size:
2112 1228 653
PciI A'CATG_T
Cuts at: 0 1877 3993
Size: 1877 2116
PflMI CCAn_nnn'nTGG
Cuts at: 0 3646 3916 3993
Size: 3646 270 77
Fragments arranged by size:
3646 270 77
PfoI T'CCnGG_A
Cuts at: 0 3474 3993
Size: 3474 519
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 923 955 3993
Size: 923 32 3038
Fragments arranged by size:
3038 923 32
PpuMI rG'GwC_Cy
Cuts at: 0 3216 3718 3993
Size: 3216 502 275
Fragments arranged by size:
3216 502 275
PsiI TTA'TAA
Cuts at: 0 559 860 3993
Size: 559 301 3133
Fragments arranged by size:
3133 559 301
PspXI vC'TCGA_Gb
Cuts at: 0 2648 3993
Size: 2648 1345
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 359 391 3993
Size: 359 32 3602
Fragments arranged by size:
3602 359 32
PstI C_TGCA'G
Cuts at: 0 1461 2733 2958 3136 3306 3395 3716 3993
Size: 1461 1272 225 178 170 89 321 277
Fragments arranged by size:
1461 1272 321 277 225 178 170 89
PvuII CAG'CTG
Cuts at: 0 483 2268 2453 3859 3993
Size: 483 1785 185 1406 134
Fragments arranged by size:
1785 1406 483 185 134
SanDI GG'GwC_CC
Cuts at: 0 3718 3993
Size: 3718 275
SapI GCTCTTCn'nnn_
Cuts at: 0 649 2121 3993
Size: 649 1472 1872
Fragments arranged by size:
1872 1472 649
SbfI CC_TGCA'GG
Cuts at: 0 3716 3993
Size: 3716 277
SexAI A'CCwGG_T
Cuts at: 0 1884 2796 3993
Size: 1884 912 1197
Fragments arranged by size:
1884 1197 912
SfcI C'TryA_G
Cuts at: 0 926 1457 2380 2729 2954 3132 3302 3391
Size: 926 531 923 349 225 178 170 89
Cuts at: 3391 3712 3993
Size: 321 281
Fragments arranged by size:
926 923 531 349 321 281 225 178
170 89
SmlI C'TyrA_G
Cuts at: 0 2648 3140 3993
Size: 2648 492 853
Fragments arranged by size:
2648 853 492
SnaBI TAC'GTA
Cuts at: 0 1837 3993
Size: 1837 2156
SpeI A'CTAG_T
Cuts at: 0 1467 3993
Size: 1467 2526
SphI G_CATG'C
Cuts at: 0 1228 3993
Size: 1228 2765
StuI AGG'CCT
Cuts at: 0 3871 3993
Size: 3871 122
StyI C'CwwG_G
Cuts at: 0 806 1271 1607 3003 3212 3673 3993
Size: 806 465 336 1396 209 461 320
Fragments arranged by size:
1396 806 465 461 336 320 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2306 3993
Size: 2306 1687
TatI w'GTAC_w
Cuts at: 0 338 351 727 1857 3993
Size: 338 13 376 1130 2136
Fragments arranged by size:
2136 1130 376 338 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1833 1865 1937 1969 3410 3442 3568 3600
Size: 1833 32 72 32 1441 32 126 32
Cuts at: 3600 3771 3803 3912 3944 3993
Size: 171 32 109 32 49
Fragments arranged by size:
1833 1441 171 126 109 72 49 32
32 32 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1659 3993
Size: 1659 2334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2162 3594 3643 3993
Size: 2162 1432 49 350
Fragments arranged by size:
2162 1432 350 49
XhoI C'TCGA_G
Cuts at: 0 2648 3993
Size: 2648 1345
ZraI GAC'GTC
Cuts at: 0 1966 3993
Size: 1966 2027
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AhdI AlfI AloI
AlwNI ApaLI ApoI AseI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI
BsrGI BssSI BstEII BstYI BstZ17I Bsu36I BtgI BtsI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I HincII HindIII MmeI MscI
MslI MspA1I NcoI NheI NsiI NspI PciI PflMI
PfoI PpiI PpuMI PsiI PspXI PsrI PstI PvuII
SanDI SapI SbfI SexAI SfcI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI XbaI XcmI
XhoI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AleI ApaI AscI
AsiSI AvrII BaeI BbeI BglII BsiWI BspEI BspMI
BsrBI BsrFI BssHII BstAPI BstBI BstXI BtgZI ClaI
CspCI DraI EcoICRI EcoRV FalI FseI FspI FspAI
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NotI NruI PacI PasI PmeI PmlI PshAI
PspOMI PvuI RsrII SacI SacII SalI ScaI SfiI
SfoI SgfI SgrAI SmaI SrfI SspI SwaI Tth111I
XmaI XmnI