Restriction map for pF1KA1741
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AflIII      
AgeI        Alw44I      ApaLI       AscI        AseI        
AsnI        Asp700I     AspI        AviII       AvrII       
BciVI       BclI        BfrI        BlnI        BsaBI       
BsaWI       BseAI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      Bst1107I    
BstZ17I     ClaI        CpoI        DraI        EagI        
EclXI       Eco52I      EcoRV       EcoT22I     FbaI        
FspI        HpaI        MamI        MfeI        MluI        
MroI        MunI        NdeI        NotI        NsiI        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SbfI        ScaI        SexAI       SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccI            9  GTCTAC               GT^[AC][GT]AC
AhdI         2125  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        2125  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          494  TGGCCA               TGG^CCA
BamHI        3067  GGATCC               G^GATCC
BbeI         4044  GGCGCC               GGCGC^C
BbrPI        3100  CACGTG               CAC^GTG
BglII        2178  AGATCT               A^GATCT
BsaAI        3100  CACGTG               [CT]AC^GT[GA]
BsiEI         537  CGACCG               CG[GA][CT]^CG
BssSI        1443  CACGAG               CACGAG(-5/-1)
BstAPI       4465  GCAGGTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3963  GGTGACC              G^GT[ACGT]ACC
BstPI        3963  GGTGACC              G^GT[ACGT]ACC
Eam1105I     2125  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      3963  GGTGACC              G^GT[ACGT]ACC
EcoRI        2596  GAATTC               G^AATTC
FseI         3049  GGCCGGCC             GGCCGG^CC
KasI         4044  GGCGCC               G^GCGCC
KspI         5045  CCGCGG               CCGC^GG
MluNI         494  TGGCCA               TGG^CCA
MscI          494  TGGCCA               TGG^CCA
MslI          474  CACCACGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI         3050  GCCGGC               GCC^GGC
NarI         4044  GGCGCC               GG^CGCC
NgoMIV       3050  GCCGGC               G^CCGGC
NheI         3157  GCTAGC               G^CTAGC
NruI          583  TCGCGA               TCG^CGA
PmaCI        3100  CACGTG               CAC^GTG
PmlI         3100  CACGTG               CAC^GTG
SacII        5045  CCGCGG               CCGC^GG
SfiI         4429  GGCCTTGGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         4044  GGCGCC               GGC^GCC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Acc65I       2620  GGTACC               G^GTACC
             4774  GGTACC              
AcyI         2911  GACGCC               G[GA]^CG[CT]C
             4044  GGCGCC              
Aor51HI       461  AGCGCT               AGC^GCT
             2863  AGCGCT              
Asp718I      2620  GGTACC               G^GTACC
             4774  GGTACC              
BcgI          772  GCAGCCAGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4795  GCAGCAGACTCG        
BsaHI        2911  GACGCC               G[GA]^CG[CT]C
             4044  GGCGCC              
BsrFI        3050  GCCGGC               [GA]^CCGG[CT]
             3616  ACCGGC              
BstBI        1426  TTCGAA               TT^CGAA
             5058  TTCGAA              
Cfr10I       3050  GCCGGC               [GA]^CCGG[CT]
             3616  ACCGGC              
DraIII       2078  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3439  CACTTTGTG           
DrdI         3771  GACTGACTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4899  GACACGGATGTC        
Eco47III      461  AGCGCT               AGC^GCT
             2863  AGCGCT              
HgaI         1243  GACGC                GACGC(5/10)
             2911  GACGC               
Hin1I        2911  GACGCC               G[GA]^CG[CT]C
             4044  GGCGCC              
HincII       1679  GTCAAC               GT[CT]^[GA]AC
             4933  GTCAAC              
HindII       1679  GTCAAC               GT[CT]^[GA]AC
             4933  GTCAAC              
HindIII       971  AAGCTT               A^AGCTT
             2925  AAGCTT              
KpnI         2620  GGTACC               GGTAC^C
             4774  GGTACC              
NcoI           32  CCATGG               C^CATGG
             2742  CCATGG              
NspI          245  ACATGC               [GA]CATG^[CT]
             1646  GCATGT              
NspV         1426  TTCGAA               TT^CGAA
             5058  TTCGAA              
PshAI        1793  GACAGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2296  GACCCAGGTC          
SacI         2946  GAGCTC               GAGCT^C
             4060  GAGCTC              
SfuI         1426  TTCGAA               TT^CGAA
             5058  TTCGAA              


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        ApoI        
AspHI       AvaI        AvaII       BanI        BanII       
BbsI        BbvCI       BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmaCI       XmaI        
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