Restriction map for
pF1KA1726
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccII AccIII AclI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AspI AviII BanII BbrPI
BbsI BbvCI BcgI BglII BlpI
Bpu1102I BpuAI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV FseI FspI
HpaI KspI MluI MroI MvnI
NaeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacI SacII SapI SbfI ScaI
SfiI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 2366 GGTACC G^GTACC
AflII 511 CTTAAG C^TTAAG
AflIII 82 ACACGT A^C[GA][CT]GT
AgeI 1937 ACCGGT A^CCGGT
AlwNI 1328 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1651 CCTCAGG CC^T[ACGT]AGG
AseI 1125 ATTAAT AT^TAAT
AsnI 1125 ATTAAT AT^TAAT
Asp718I 2366 GGTACC G^GTACC
AvaI 2496 CCCGAG C^[CT]CG[GA]G
BalI 2234 TGGCCA TGG^CCA
BamHI 2465 GGATCC G^GATCC
BbeI 2149 GGCGCC GGCGC^C
BfrI 511 CTTAAG C^TTAAG
BsaBI 2523 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1937 ACCGGT [AT]^CCGG[AT]
BsgI 2125 CTGCAC GTGCAG(16/14)
BsoBI 2496 CCCGAG C^[CT]CG[GA]G
BssSI 2238 CACGAG CACGAG(-5/-1)
BstXI 2621 CCATTTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1651 CCTCAGG CC^T[ACGT]AGG
DraI 421 TTTAAA TTT^AAA
DraII 2224 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 1747 GACTCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 777 CCATGG C^C[GA][CT]GG
Eco81I 1651 CCTCAGG CC^T[ACGT]AGG
EcoO109I 2224 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1784 ATGCAT ATGCA^T
FauI 2309 CCCGC CCCGC(4/6)
HaeII 2149 GGCGCC [GA]GCGC^[CT]
HindIII 1519 AAGCTT A^AGCTT
KasI 2149 GGCGCC G^GCGCC
KpnI 2366 GGTACC GGTAC^C
MamI 2523 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 307 CAATTG C^AATTG
MluNI 2234 TGGCCA TGG^CCA
MscI 2234 TGGCCA TGG^CCA
MunI 307 CAATTG C^AATTG
NarI 2149 GGCGCC GG^CGCC
NcoI 777 CCATGG C^CATGG
NdeI 1253 CATATG CA^TATG
NsiI 1784 ATGCAT ATGCA^T
NspI 1994 ACATGC [GA]CATG^[CT]
PinAI 1937 ACCGGT A^CCGGT
PpuMI 2224 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1491 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1125 ATTAAT AT^TAAT
SalI 1756 GTCGAC G^TCGAC
SexAI 1546 ACCAGGT A^CC[AT]GGT
SfoI 2149 GGCGCC GGC^GCC
SnaBI 929 TACGTA TAC^GTA
SspI 1289 AATATT AAT^ATT
StuI 2319 AGGCCT AGG^CCT
XbaI 1354 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1498 GTCTAC GT^[AC][GT]AC
1756 GTCGAC
AspHI 147 GAGCAC G[AT]GC[AT]^C
1457 GTGCTC
AvrII 114 CCTAGG C^CTAGG
2217 CCTAGG
BciVI 207 GGATAC GTATCC(6/5)
1734 GTATCC
BclI 291 TGATCA T^GATCA
627 TGATCA
BcnI 971 CCCGG CC^[GC]GG
2153 CCCGG
BlnI 114 CCTAGG C^CTAGG
2217 CCTAGG
BmyI 147 GAGCAC G[AGT]GC[ACT]^C
1457 GTGCTC
BpmI 499 CTGGAG CTGGAG(16/14)
1634 CTGGAG
BsaI 738 GAGACC GGTCTC(1/5)
368 GGTCTC
BsiHKAI 147 GAGCAC G[AT]GC[AT]^C
1457 GTGCTC
Bsp1286I 147 GAGCAC G[AGT]GC[ACT]^C
1457 GTGCTC
BspHI 294 TCATGA T^CATGA
2573 TCATGA
BspMI 2129 ACCTGC ACCTGC(4/8)
365 GCAGGT
BsrFI 1937 ACCGGT [GA]^CCGG[CT]
2369 ACCGGC
CfoI 399 GCGC GCG^C
2150 GCGC
Cfr10I 1937 ACCGGT [GA]^CCGG[CT]
2369 ACCGGC
EaeI 129 CGGCCA [CT]^GGCC[GA]
2234 TGGCCA
FbaI 291 TGATCA T^GATCA
627 TGATCA
HhaI 399 GCGC GCG^C
2150 GCGC
HinP1I 399 GCGC G^CGC
2150 GCGC
NciI 971 CCCGG CC^[GC]GG
2153 CCCGG
PflMI 2341 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2470 CCATACTGTGG
RcaI 294 TCATGA T^CATGA
2573 TCATGA
SmlI 394 CTCAAG C^T[CT][GA]AG
511 CTTAAG
Van91I 2341 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2470 CCATACTGTGG
XcmI 169 CCAGCTGTACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1529 CCAAAAACAAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI Asp700I AvaII BanI
BbvI BfaI BglI BsaAI BsaHI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsmI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI