Restriction map for pF1KA1726
 (Linear) (Six-base) MAPSORT of: KIAA1726.seq  Check: 3515  from: 1  to: 2652



KIAA1726.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2138    2652 
   Size:       2138     514
 
AccI GT'mk_AC

Cuts at:      0    1499    1757    2652 
   Size:       1499     258     895

  Fragments arranged by size: 

                1499     895     258
 
Acc65I G'GTAC_C

Cuts at:      0    2366    2652 
   Size:       2366     286
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     293     449     548    2652 
   Size:        266      27     156      99    2104

  Fragments arranged by size: 

                2104     266     156      99      27
 
AflII C'TTAA_G

Cuts at:      0     511    2652 
   Size:        511    2141
 
AflIII A'CryG_T

Cuts at:      0      82    2652 
   Size:         82    2570
 
AgeI A'CCGG_T

Cuts at:      0    1937    2652 
   Size:       1937     715
 
AleI CACnn'nnGTG

Cuts at:      0    2406    2652 
   Size:       2406     246
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2075    2107    2652 
   Size:       2075      32     545

  Fragments arranged by size: 

                2075     545      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1333    2652 
   Size:       1333    1319
 
ApoI r'AATT_y

Cuts at:      0     285     407     923    1977    2037    2652 
   Size:        285     122     516    1054      60     615

  Fragments arranged by size: 

                1054     615     516     285     122      60
 
AseI AT'TA_AT

Cuts at:      0    1126    2652 
   Size:       1126    1526
 
AvaI C'yCGr_G

Cuts at:      0    2496    2652 
   Size:       2496     156
 
AvrII C'CTAG_G

Cuts at:      0     114    2217    2652 
   Size:        114    2103     435

  Fragments arranged by size: 

                2103     435     114
 
BamHI G'GATC_C

Cuts at:      0    2465    2652 
   Size:       2465     187
 
BanI G'GyrC_C

Cuts at:      0    2149    2208    2366    2652 
   Size:       2149      59     158     286

  Fragments arranged by size: 

                2149     286     158      59
 
BbeI G_GCGC'C

Cuts at:      0    2153    2652 
   Size:       2153     499
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     201    1745    2652 
   Size:        201    1544     907

  Fragments arranged by size: 

                1544     907     201
 
BclI T'GATC_A

Cuts at:      0     291     627    2652 
   Size:        291     336    2025

  Fragments arranged by size: 

                2025     336     291
 
BfrBI ATG'CAT

Cuts at:      0    1786    2652 
   Size:       1786     866
 
BglI GCCn_nnn'nGGC

Cuts at:      0     972    2318    2460    2652 
   Size:        972    1346     142     192

  Fragments arranged by size: 

                1346     972     192     142
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1184    2652 
   Size:       1184    1468
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520    1655    2652 
   Size:        520    1135     997

  Fragments arranged by size: 

                1135     997     520
 
Bpu10I CC'TnA_GC

Cuts at:      0    2441    2652 
   Size:       2441     211
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379    2652 
   Size:        379    2273
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     374     732    2652 
   Size:        374     358    1920

  Fragments arranged by size: 

                1920     374     358
 
BsaAI yAC'GTr

Cuts at:      0      85     931    1923    2652 
   Size:         85     846     992     729

  Fragments arranged by size: 

                 992     846     729      85
 
BsaBI GATnn'nnATC

Cuts at:      0    2527    2652 
   Size:       2527     125
 
BsaHI Gr'CG_yC

Cuts at:      0     241    2150    2357    2600    2652 
   Size:        241    1909     207     243      52

  Fragments arranged by size: 

                1909     243     241     207      52
 
BsaWI w'CCGG_w

Cuts at:      0    1937    2652 
   Size:       1937     715
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     584     614    1379    1409    2652 
   Size:        584      30     765      30    1243

  Fragments arranged by size: 

                1243     765     584      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1002    1987    2096    2174    2652 
   Size:       1002     985     109      78     478

  Fragments arranged by size: 

                1002     985     478     109      78
 
BseYI C'CCAG_C

Cuts at:      0    1575    2330    2603    2639    2643    2652 
   Size:       1575     755     273      36       4       9

  Fragments arranged by size: 

                1575     755     273      36       9       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2110    2652 
   Size:       2110     542
 
BsiHKAI G_wGCw'C

Cuts at:      0     151    1461    2652 
   Size:        151    1310    1191

  Fragments arranged by size: 

                1310    1191     151
 
BsmI GAATG_Cn'

Cuts at:      0      23      41     258    1788    2486    2652 
   Size:         23      18     217    1530     698     166

  Fragments arranged by size: 

                1530     698     217     166      23      18
 
Bsp1286I G_dGCh'C

Cuts at:      0     151    1461    2652 
   Size:        151    1310    1191

  Fragments arranged by size: 

                1310    1191     151
 
BspHI T'CATG_A

Cuts at:      0     294    2573    2652 
   Size:        294    2279      79

  Fragments arranged by size: 

                2279     294      79
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     356    2138    2652 
   Size:        356    1782     514

  Fragments arranged by size: 

                1782     514     356
 
BsrDI GCAATG_nn'

Cuts at:      0     408     768     776    1626    2652 
   Size:        408     360       8     850    1026

  Fragments arranged by size: 

                1026     850     408     360       8
 
BsrFI r'CCGG_y

Cuts at:      0    1937    2369    2652 
   Size:       1937     432     283

  Fragments arranged by size: 

                1937     432     283
 
BssSI C'ACGA_G

Cuts at:      0    2238    2652 
   Size:       2238     414
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2628    2652 
   Size:       2628      24
 
BstYI r'GATC_y

Cuts at:      0    1476    2465    2490    2652 
   Size:       1476     989      25     162

  Fragments arranged by size: 

                1476     989     162      25
 
Bsu36I CC'TnA_GG

Cuts at:      0    1652    2652 
   Size:       1652    1000
 
BtgI C'CryG_G

Cuts at:      0     777    2652 
   Size:        777    1875
 
BtsI GCAGTG_nn'

Cuts at:      0     827    1890    1917    2652 
   Size:        827    1063      27     735

  Fragments arranged by size: 

                1063     827     735      27
 
DraI TTT'AAA

Cuts at:      0     423    2652 
   Size:        423    2229
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1753    2652 
   Size:       1753     899
 
EaeI y'GGCC_r

Cuts at:      0     129    2234    2652 
   Size:        129    2105     418

  Fragments arranged by size: 

                2105     418     129
 
EarI CTCTTCn'nnn_

Cuts at:      0       7     242     425    2114    2652 
   Size:          7     235     183    1689     538

  Fragments arranged by size: 

                1689     538     235     183       7
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2478    2652 
   Size:       2478     174
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     293     449     520     548    1655    2652 
   Size:        266      27     156      71      28    1107     997

  Fragments arranged by size: 

                1107     997     266     156      71      28      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2225    2652 
   Size:       2225     427
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1537    2318    2652 
   Size:       1537     781     334

  Fragments arranged by size: 

                1537     781     334
 
HaeII r_GCGC'y

Cuts at:      0    2153    2652 
   Size:       2153     499
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     283     315     584     616    2164    2196    2239    2271 
   Size:        283      32     269      32    1548      32      43      32

Cuts at:   2271    2652 
   Size:        381

  Fragments arranged by size: 

                1548     381     283     269      43      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     185    1758    1990    2652 
   Size:        185    1573     232     662

  Fragments arranged by size: 

                1573     662     232     185
 
HindIII A'AGCT_T

Cuts at:      0    1519    2652 
   Size:       1519    1133
 
KasI G'GCGC_C

Cuts at:      0    2149    2652 
   Size:       2149     503
 
KpnI G_GTAC'C

Cuts at:      0    2370    2652 
   Size:       2370     282
 
MfeI C'AATT_G

Cuts at:      0     307    2652 
   Size:        307    2345
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1019    2652 
   Size:       1019    1633
 
MscI TGG'CCA

Cuts at:      0    2236    2652 
   Size:       2236     416
 
MslI CAynn'nnrTG

Cuts at:      0     137    2406    2626    2652 
   Size:        137    2269     220      26

  Fragments arranged by size: 

                2269     220     137      26
 
MspA1I CmG'CkG

Cuts at:      0     172    1323    2143    2162    2643    2652 
   Size:        172    1151     820      19     481       9

  Fragments arranged by size: 

                1151     820     481     172      19       9
 
NarI GG'CG_CC

Cuts at:      0    2150    2652 
   Size:       2150     502
 
NcoI C'CATG_G

Cuts at:      0     777    2652 
   Size:        777    1875
 
NdeI CA'TA_TG

Cuts at:      0    1254    2652 
   Size:       1254    1398
 
NsiI A_TGCA'T

Cuts at:      0    1788    2652 
   Size:       1788     864
 
NspI r_CATG'y

Cuts at:      0    1998    2652 
   Size:       1998     654
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2347    2476    2652 
   Size:       2347     129     176

  Fragments arranged by size: 

                2347     176     129
 
PpuMI rG'GwC_Cy

Cuts at:      0    2225    2652 
   Size:       2225     427
 
PshAI GACnn'nnGTC

Cuts at:      0    1495    2652 
   Size:       1495    1157
 
PstI C_TGCA'G

Cuts at:      0     367     805    1882    2036    2652 
   Size:        367     438    1077     154     616

  Fragments arranged by size: 

                1077     616     438     367     154
 
PvuII CAG'CTG

Cuts at:      0     172    2162    2643    2652 
   Size:        172    1990     481       9

  Fragments arranged by size: 

                1990     481     172       9
 
SalI G'TCGA_C

Cuts at:      0    1756    2652 
   Size:       1756     896
 
SexAI A'CCwGG_T

Cuts at:      0    1546    2652 
   Size:       1546    1106
 
SfcI C'TryA_G

Cuts at:      0     363     801    1068    1878    2032    2652 
   Size:        363     438     267     810     154     620

  Fragments arranged by size: 

                 810     620     438     363     267     154
 
SfoI GGC'GCC

Cuts at:      0    2151    2652 
   Size:       2151     501
 
SmlI C'TyrA_G

Cuts at:      0     394     511    2652 
   Size:        394     117    2141

  Fragments arranged by size: 

                2141     394     117
 
SnaBI TAC'GTA

Cuts at:      0     931    2652 
   Size:        931    1721
 
SspI AAT'ATT

Cuts at:      0    1291    2652 
   Size:       1291    1361
 
StuI AGG'CCT

Cuts at:      0    2321    2652 
   Size:       2321     331
 
StyI C'CwwG_G

Cuts at:      0     114     178     449     777    1424    2217    2228    2652 
   Size:        114      64     271     328     647     793      11     424

  Fragments arranged by size: 

                 793     647     424     328     271     114      64      11
            
 
TatI w'GTAC_w

Cuts at:      0      79     546     640    2652 
   Size:         79     467      94    2012

  Fragments arranged by size: 

                2012     467      94      79
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1379    1411    2652 
   Size:       1379      32    1241

  Fragments arranged by size: 

                1379    1241      32
 
XbaI T'CTAG_A

Cuts at:      0    1354    2652 
   Size:       1354    1298
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     176    1536    2652 
   Size:        176    1360    1116

  Fragments arranged by size: 

                1360    1116     176
 
XmnI GAAnn'nnTTC

Cuts at:      0     536    1105    1520    1860    2652 
   Size:        536     569     415     340     792

  Fragments arranged by size: 

                 792     569     536     415     340
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI    AflII   AflIII     AgeI     AleI
     AloI    AlwNI     ApoI     AseI     AvaI    AvrII    BamHI     BanI
     BbeI    BciVI     BclI    BfrBI     BglI     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI
    BsrFI    BssSI    BstXI    BstYI   Bsu36I     BtgI     BtsI     DraI
     DrdI     EaeI     EarI     EciI  Eco57MI EcoO109I     FalI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MfeI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NdeI     NsiI     NspI    PflMI
    PpuMI    PshAI     PstI    PvuII     SalI    SexAI     SfcI     SfoI
     SmlI    SnaBI     SspI     StuI     StyI     TatI     TstI     XbaI
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI     AhdI     AlfI     ApaI    ApaLI     AscI
    AsiSI     BaeI    BanII     BbsI    BbvCI     BcgI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI    BsiEI    BsiWI    BsmBI    BspEI
    BsrBI    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I    BtgZI
     ClaI    CspCI   DraIII     EagI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI     HpaI     MluI     NaeI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII    SanDI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI
     SmaI     SpeI     SphI     SrfI     SwaI    TaqII  Tth111I     XhoI
     XmaI     ZraI
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