Restriction map for pF1KA1717
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        AspEI       AspI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HpaI        KasI        KspI        MaeI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspA1I      MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacII       
SbfI        SfcI        SfoI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     Van91I      XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I       1055  GGTACC               G^GTACC
AlwI          625  GATCC                GGATC(4/5)
AlwNI         771  CAGGGACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I      1055  GGTACC               G^GTACC
AspHI         988  GAGCTC               G[AT]GC[AT]^C
AvaI         1024  CCCGGG               C^[CT]CG[GA]G
BbvI          167  GCAGC                GCAGC(8/12)
BciVI         850  GTATCC               GTATCC(6/5)
BglI         1075  GCCTTCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI           75  CTGCAC               GTGCAG(16/14)
BsiHKAI       988  GAGCTC               G[AT]GC[AT]^C
BsmAI         306  GAGAC                GTCTC(1/5)
BsoBI        1024  CCCGGG               C^[CT]CG[GA]G
BstBI         743  TTCGAA               TT^CGAA
BstYI         624  AGATCC               [GA]^GATC[CT]
EcoT14I       869  CCTTGG               C^C[AT][AT]GG
EcoT22I       951  ATGCAT               ATGCA^T
Fnu4HI        167  GCAGC                GC^[ACGT]GC
HapII        1025  CCGG                 C^CGG
HpaII        1025  CCGG                 C^CGG
ItaI          167  GCAGC                GC^[ACGT]GC
KpnI         1055  GGTACC               GGTAC^C
MaeIII         82  GTCAC                ^GT[ACGT]AC
MflI          624  AGATCC               [GA]^GATC[CT]
MseI          785  TTAA                 T^TAA
MslI          822  CATTGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI         1025  CCGG                 C^CGG
NlaIII        346  CATG                 CATG^
NsiI          951  ATGCAT               ATGCA^T
NspV          743  TTCGAA               TT^CGAA
PpuMI         708  GGGACCT              [GA]G^G[AT]CC[CT]
SacI          988  GAGCTC               GAGCT^C
SalI          585  GTCGAC               G^TCGAC
ScaI          857  AGTACT               AGT^ACT
SexAI        1058  ACCAGGT              A^CC[AT]GGT
SfiI         1074  GGCCTTCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          743  TTCGAA               TT^CGAA
SmaI         1024  CCCGGG               CCC^GGG
StyI          869  CCTTGG               C^C[AT][AT]GG
Tru9I         785  TTAA                 T^TAA
TseI          167  GCAGC                G^C[AT]GC
Tsp45I         82  GTCAC                ^GT[GC]AC
XcmI          927  CCACCCCCGTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         624  AGATCC               [GA]^GATC[CT]
XmaCI        1024  CCCGGG               C^CCGGG
XmaI         1024  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccI          259  GTAGAC               GT^[AC][GT]AC
              585  GTCGAC              
AcsI          472  AAATTT               [GA]^AATT[CT]
              561  AAATTC              
ApaI          779  GGGCCC               GGGCC^C
              940  GGGCCC              
ApoI          472  AAATTT               [GA]^AATT[CT]
              561  AAATTC              
Asp700I       147  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1071  GAAGGCCTTC          
BanI           41  GGCACC               G^G[CT][GA]CC
             1055  GGTACC              
BcnI         1024  CCCGG                CC^[GC]GG
             1025  CCGGG               
BsaAI         252  CACGTA               [CT]AC^GT[GA]
              847  CACGTA              
BseRI          90  CTCCTC               GAGGAG(10/8)
               25  GAGGAG              
BstF5I        954  CATCC                GGATG(2/0)
              192  GGATG               
DpnII         312  GATC                 ^GATC
              625  GATC                
DraII         708  GGGACCT              [GA]G^G[ACGT]CC[CT]
              779  GGGCCCT             
EcoO109I      708  GGGACCT              [GA]G^G[ACGT]CC[CT]
              779  GGGCCCT             
FokI          954  CATCC                GGATG(9/13)
              192  GGATG               
HincII        585  GTCGAC               GT[CT]^[GA]AC
              763  GTTGAC              
HindII        585  GTCGAC               GT[CT]^[GA]AC
              763  GTTGAC              
HinfI         634  GAATC                G^A[ACGT]TC
              658  GAATC               
HphI           83  TCACC                GGTGA(8/7)
              992  TCACC               
MaeII         253  ACGT                 A^CGT
              848  ACGT                
MboI          312  GATC                 ^GATC
              625  GATC                
MwoI          970  GCAGTGGAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1075  GCCTTCCAGGC         
NciI         1024  CCCGG                CC^[GC]GG
             1025  CCGGG               
NdeII         312  GATC                 ^GATC
              625  GATC                
SapI          985  GAAGAGC              GCTCTTC(1/4)
              613  GCTCTTC             
Sau3AI        312  GATC                 ^GATC
              625  GATC                
StuI          535  AGGCCT               AGG^CCT
             1073  AGGCCT              
TaqI          586  TCGA                 T^CGA
              744  TCGA                
TfiI          634  GAATC                G^A[AT]TC
              658  GAATC               
TthHB8I       586  TCGA                 T^CGA
              744  TCGA                
XmnI          147  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1071  GAAGGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BanII       
BmyI        BpmI        BsaJI       BsiYI       BslI        
BsmFI       Bsp1286I    BsrI        BssKI       BstNI       
Cfr13I      DdeI        EarI        EcoRII      HaeIII      
Ksp632I     MboII       MnlI        MvaI        NlaIV       
RsaI        Sau96I      ScrFI       SfaNI       Tsp509I     
TspRI       
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