Restriction map for
pF1KA1708
(Linear) (Six-base) MAPSORT of: KIAA1708.seq Check: 202 from: 1 to: 4983
KIAA1708.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1393 2541 4983
Size: 1393 1148 2442
Fragments arranged by size:
2442 1393 1148
AccI GT'mk_AC
Cuts at: 0 3162 4411 4983
Size: 3162 1249 572
Fragments arranged by size:
3162 1249 572
AclI AA'CG_TT
Cuts at: 0 1072 4983
Size: 1072 3911
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1034 1171 2462 3103 3356 3461 3740
Size: 862 172 137 1291 641 253 105 279
Cuts at: 3740 3955 4010 4226 4672 4983
Size: 215 55 216 446 311
Fragments arranged by size:
1291 862 641 446 311 279 253 216
215 172 137 105 55
AflIII A'CryG_T
Cuts at: 0 574 957 1259 4983
Size: 574 383 302 3724
Fragments arranged by size:
3724 574 383 302
AhdI GACnn_n'nnGTC
Cuts at: 0 4556 4983
Size: 4556 427
AleI CACnn'nnGTG
Cuts at: 0 3972 4983
Size: 3972 1011
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1175 4983
Size: 1175 3808
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2502 2534 4983
Size: 2502 32 2449
Fragments arranged by size:
2502 2449 32
AlwNI CAG_nnn'CTG
Cuts at: 0 832 1702 1864 1980 2563 3748 4766 4983
Size: 832 870 162 116 583 1185 1018 217
Fragments arranged by size:
1185 1018 870 832 583 217 162 116
ApoI r'AATT_y
Cuts at: 0 527 551 784 2024 2609 3662 4907 4983
Size: 527 24 233 1240 585 1053 1245 76
Fragments arranged by size:
1245 1240 1053 585 527 233 76 24
AseI AT'TA_AT
Cuts at: 0 3047 4983
Size: 3047 1936
AvaI C'yCGr_G
Cuts at: 0 344 1006 4983
Size: 344 662 3977
Fragments arranged by size:
3977 662 344
AvrII C'CTAG_G
Cuts at: 0 126 4434 4983
Size: 126 4308 549
Fragments arranged by size:
4308 549 126
BanI G'GyrC_C
Cuts at: 0 7 4348 4737 4983
Size: 7 4341 389 246
Fragments arranged by size:
4341 389 246 7
BanII G_rGCy'C
Cuts at: 0 25 452 670 1199 2595 3593 4545 4983
Size: 25 427 218 529 1396 998 952 438
Fragments arranged by size:
1396 998 952 529 438 427 218 25
BbeI G_GCGC'C
Cuts at: 0 11 4983
Size: 11 4972
BbsI GAAGACnn'nnnn_
Cuts at: 0 114 3109 3362 4158 4232 4983
Size: 114 2995 253 796 74 751
Fragments arranged by size:
2995 796 751 253 114 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2557 2591 4983
Size: 2557 34 2392
Fragments arranged by size:
2557 2392 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 229 2025 4794 4983
Size: 229 1796 2769 189
Fragments arranged by size:
2769 1796 229 189
BclI T'GATC_A
Cuts at: 0 1374 1785 4983
Size: 1374 411 3198
Fragments arranged by size:
3198 1374 411
BfrBI ATG'CAT
Cuts at: 0 1342 2578 4983
Size: 1342 1236 2405
Fragments arranged by size:
2405 1342 1236
BglII A'GATC_T
Cuts at: 0 188 1461 3279 3375 4482 4940 4956 4983
Size: 188 1273 1818 96 1107 458 16 27
Fragments arranged by size:
1818 1273 1107 458 188 96 27 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 340 4097 4117 4744 4983
Size: 340 3757 20 627 239
Fragments arranged by size:
3757 627 340 239 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 401 566 872 3897 4328 4983
Size: 290 111 165 306 3025 431 655
Fragments arranged by size:
3025 655 431 306 290 165 111
Bpu10I CC'TnA_GC
Cuts at: 0 2537 3245 4983
Size: 2537 708 1738
Fragments arranged by size:
2537 1738 708
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2758 2797 4983
Size: 2758 39 2186
Fragments arranged by size:
2758 2186 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 2042 4983
Size: 2042 2941
BsaAI yAC'GTr
Cuts at: 0 577 4983
Size: 577 4406
BsaHI Gr'CG_yC
Cuts at: 0 8 4983
Size: 8 4975
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 7 37 157 187 1554 1584 3895 3925
Size: 7 30 120 30 1367 30 2311 30
Cuts at: 3925 4983
Size: 1058
Fragments arranged by size:
2311 1367 1058 120 30 30 30 30
7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1432 1819 1822 1825 1828 1831 2800 3876
Size: 1432 387 3 3 3 3 969 1076
Cuts at: 3876 4027 4983
Size: 151 956
Fragments arranged by size:
1432 1076 969 956 387 151 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 176 2494 2594 4983
Size: 176 2318 100 2389
Fragments arranged by size:
2389 2318 176 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2576 3039 4983
Size: 2576 463 1944
Fragments arranged by size:
2576 1944 463
BsiHKAI G_wGCw'C
Cuts at: 0 25 452 670 1199 3593 4013 4545 4983
Size: 25 427 218 529 2394 420 532 438
Fragments arranged by size:
2394 532 529 438 427 420 218 25
BsmI GAATG_Cn'
Cuts at: 0 438 1278 4223 4983
Size: 438 840 2945 760
Fragments arranged by size:
2945 840 760 438
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1324 4983
Size: 1324 3659
Bsp1286I G_dGCh'C
Cuts at: 0 25 452 670 1199 2595 3593 4013 4545
Size: 25 427 218 529 1396 998 420 532
Cuts at: 4545 4983
Size: 438
Fragments arranged by size:
1396 998 532 529 438 427 420 218
25
BspHI T'CATG_A
Cuts at: 0 531 1028 1266 1788 3380 4833 4983
Size: 531 497 238 522 1592 1453 150
Fragments arranged by size:
1592 1453 531 522 497 238 150
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1310 1393 2541 3086 4983
Size: 1310 83 1148 545 1897
Fragments arranged by size:
1897 1310 1148 545 83
BsrDI GCAATG_nn'
Cuts at: 0 921 4983
Size: 921 4062
BsrFI r'CCGG_y
Cuts at: 0 2643 3794 4983
Size: 2643 1151 1189
Fragments arranged by size:
2643 1189 1151
BsrGI T'GTAC_A
Cuts at: 0 91 4983
Size: 91 4892
BssSI C'ACGA_G
Cuts at: 0 488 4983
Size: 488 4495
BstBI TT'CG_AA
Cuts at: 0 1509 4227 4983
Size: 1509 2718 756
Fragments arranged by size:
2718 1509 756
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3441 4983
Size: 3441 1542
BstYI r'GATC_y
Cuts at: 0 188 829 1461 3279 3375 3907 4392 4482
Size: 188 641 632 1818 96 532 485 90
Cuts at: 4482 4940 4956 4983
Size: 458 16 27
Fragments arranged by size:
1818 641 632 532 485 458 188 96
90 27 16
Bsu36I CC'TnA_GG
Cuts at: 0 116 4983
Size: 116 4867
BtsI GCAGTG_nn'
Cuts at: 0 613 1499 3471 4983
Size: 613 886 1972 1512
Fragments arranged by size:
1972 1512 886 613
ClaI AT'CG_AT
Cuts at: 0 2948 4983
Size: 2948 2035
DraI TTT'AAA
Cuts at: 0 426 3338 4983
Size: 426 2912 1645
Fragments arranged by size:
2912 1645 426
EaeI y'GGCC_r
Cuts at: 0 2691 4983
Size: 2691 2292
EarI CTCTTCn'nnn_
Cuts at: 0 455 1229 1796 1991 2438 2462 2915 4045
Size: 455 774 567 195 447 24 453 1130
Cuts at: 4045 4983
Size: 938
Fragments arranged by size:
1130 938 774 567 455 453 447 195
24
EcoICRI GAG'CTC
Cuts at: 0 23 450 668 1197 3591 4543 4983
Size: 23 427 218 529 2394 952 440
Fragments arranged by size:
2394 952 529 440 427 218 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 401 566 862 872 1034 1171 2462
Size: 290 111 165 296 10 162 137 1291
Cuts at: 2462 3103 3356 3461 3740 3897 3955 4010 4226
Size: 641 253 105 279 157 58 55 216
Cuts at: 4226 4328 4672 4983
Size: 102 344 311
Fragments arranged by size:
1291 641 344 311 296 290 279 253
216 165 162 157 137 111 105 102
58 55 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3915 4983
Size: 3915 1068
EcoO109I rG'GnC_Cy
Cuts at: 0 465 4438 4983
Size: 465 3973 545
Fragments arranged by size:
3973 545 465
EcoRI G'AATT_C
Cuts at: 0 527 2609 4983
Size: 527 2082 2374
Fragments arranged by size:
2374 2082 527
EcoRV GAT'ATC
Cuts at: 0 4203 4983
Size: 4203 780
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 484 3587 4273 4561 4702 4983
Size: 484 3103 686 288 141 281
Fragments arranged by size:
3103 686 484 288 281 141
HaeII r_GCGC'y
Cuts at: 0 11 1543 4983
Size: 11 1532 3440
Fragments arranged by size:
3440 1532 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 157 189 1116 1148 1852 1884
Size: 7 32 118 32 927 32 704 32
Cuts at: 1884 2914 2946 4377 4409 4542 4574 4815 4847
Size: 1030 32 1431 32 133 32 241 32
Cuts at: 4847 4983
Size: 136
Fragments arranged by size:
1431 1030 927 704 241 136 133 118
32 32 32 32 32 32 32 32
7
HincII GTy'rAC
Cuts at: 0 312 3163 4424 4983
Size: 312 2851 1261 559
Fragments arranged by size:
2851 1261 559 312
HindIII A'AGCT_T
Cuts at: 0 3385 4983
Size: 3385 1598
HpaI GTT'AAC
Cuts at: 0 312 4424 4983
Size: 312 4112 559
Fragments arranged by size:
4112 559 312
KasI G'GCGC_C
Cuts at: 0 7 4983
Size: 7 4976
MfeI C'AATT_G
Cuts at: 0 2224 4983
Size: 2224 2759
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2807 4983
Size: 2807 2176
MslI CAynn'nnrTG
Cuts at: 0 697 1271 1793 2761 3439 3972 4519 4589
Size: 697 574 522 968 678 533 547 70
Cuts at: 4589 4983
Size: 394
Fragments arranged by size:
968 697 678 574 547 533 522 394
70
MspA1I CmG'CkG
Cuts at: 0 1553 2560 4888 4983
Size: 1553 1007 2328 95
Fragments arranged by size:
2328 1553 1007 95
NaeI GCC'GGC
Cuts at: 0 2645 3796 4983
Size: 2645 1151 1187
Fragments arranged by size:
2645 1187 1151
NarI GG'CG_CC
Cuts at: 0 8 4983
Size: 8 4975
NdeI CA'TA_TG
Cuts at: 0 3215 3699 4855 4983
Size: 3215 484 1156 128
Fragments arranged by size:
3215 1156 484 128
NgoMIV G'CCGG_C
Cuts at: 0 2643 3794 4983
Size: 2643 1151 1189
Fragments arranged by size:
2643 1189 1151
NsiI A_TGCA'T
Cuts at: 0 1344 2580 4983
Size: 1344 1236 2403
Fragments arranged by size:
2403 1344 1236
NspI r_CATG'y
Cuts at: 0 682 961 1040 1263 2188 4983
Size: 682 279 79 223 925 2795
Fragments arranged by size:
2795 925 682 279 223 79
PciI A'CATG_T
Cuts at: 0 957 1259 4983
Size: 957 302 3724
Fragments arranged by size:
3724 957 302
PflMI CCAn_nnn'nTGG
Cuts at: 0 2421 4983
Size: 2421 2562
PfoI T'CCnGG_A
Cuts at: 0 631 4983
Size: 631 4352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 657 689 3420 3452 4983
Size: 657 32 2731 32 1531
Fragments arranged by size:
2731 1531 657 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 465 4983
Size: 465 4518
PshAI GACnn'nnGTC
Cuts at: 0 2478 4983
Size: 2478 2505
PstI C_TGCA'G
Cuts at: 0 1976 2159 3042 4771 4887 4983
Size: 1976 183 883 1729 116 96
Fragments arranged by size:
1976 1729 883 183 116 96
PvuII CAG'CTG
Cuts at: 0 2560 4888 4983
Size: 2560 2328 95
Fragments arranged by size:
2560 2328 95
SacI G_AGCT'C
Cuts at: 0 25 452 670 1199 3593 4545 4983
Size: 25 427 218 529 2394 952 438
Fragments arranged by size:
2394 952 529 438 427 218 25
SalI G'TCGA_C
Cuts at: 0 3161 4983
Size: 3161 1822
SapI GCTCTTCn'nnn_
Cuts at: 0 2462 4983
Size: 2462 2521
ScaI AGT'ACT
Cuts at: 0 3152 3318 4983
Size: 3152 166 1665
Fragments arranged by size:
3152 1665 166
SexAI A'CCwGG_T
Cuts at: 0 2051 4983
Size: 2051 2932
SfcI C'TryA_G
Cuts at: 0 245 857 966 994 1282 1972 2155 3038
Size: 245 612 109 28 288 690 183 883
Cuts at: 3038 4412 4767 4883 4983
Size: 1374 355 116 100
Fragments arranged by size:
1374 883 690 612 355 288 245 183
116 109 100 28
SfoI GGC'GCC
Cuts at: 0 9 4983
Size: 9 4974
SmlI C'TyrA_G
Cuts at: 0 344 2737 2812 4983
Size: 344 2393 75 2171
Fragments arranged by size:
2393 2171 344 75
SpeI A'CTAG_T
Cuts at: 0 3484 4983
Size: 3484 1499
StuI AGG'CCT
Cuts at: 0 2632 4983
Size: 2632 2351
StyI C'CwwG_G
Cuts at: 0 126 929 3725 4434 4502 4727 4983
Size: 126 803 2796 709 68 225 256
Fragments arranged by size:
2796 803 709 256 225 126 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 610 4579 4763 4885 4983
Size: 610 3969 184 122 98
Fragments arranged by size:
3969 610 184 122 98
TatI w'GTAC_w
Cuts at: 0 91 1205 3150 3316 3487 4983
Size: 91 1114 1945 166 171 1496
Fragments arranged by size:
1945 1496 1114 171 166 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3895 3927 4983
Size: 3895 32 1056
Fragments arranged by size:
3895 1056 32
Tth111I GACn'n_nGTC
Cuts at: 0 2322 4983
Size: 2322 2661
XbaI T'CTAG_A
Cuts at: 0 2326 3282 4639 4983
Size: 2326 956 1357 344
Fragments arranged by size:
2326 1357 956 344
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2784 3438 4983
Size: 2784 654 1545
Fragments arranged by size:
2784 1545 654
XhoI C'TCGA_G
Cuts at: 0 344 4983
Size: 344 4639
XmnI GAAnn'nnTTC
Cuts at: 0 2423 4983
Size: 2423 2560
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApoI AseI AvaI AvrII BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrFI BsrGI BssSI BstBI BstXI BstYI Bsu36I
BtsI ClaI DraI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
HpaI KasI MfeI MmeI MslI MspA1I NaeI NarI
NdeI NgoMIV NsiI NspI PciI PflMI PfoI PpiI
PpuMI PshAI PstI PvuII SacI SalI SapI ScaI
SexAI SfcI SfoI SmlI SpeI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI ApaI ApaLI AscI
AsiSI BaeI BamHI BbvCI BglI BlpI Bme1580I BmgBI
BmtI BplI BsaBI BsaWI BsiEI BsiWI BspEI BsrBI
BssHII BstAPI BstEII BstZ17I BtgI BtgZI CspCI DraIII
DrdI EagI EciI FseI FspI FspAI KpnI MluI
MscI NcoI NheI NotI NruI PacI PasI PmeI
PmlI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI SphI
SrfI SspI SwaI XmaI ZraI