Restriction map for
pF1KA1707
(Linear) (Six-base) MAPSORT of: KIAA1707.seq Check: 7267 from: 1 to: 3051
KIAA1707.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 980 1664 1997 3051
Size: 713 267 684 333 1054
Fragments arranged by size:
1054 713 684 333 267
AflII C'TTAA_G
Cuts at: 0 1273 2254 3051
Size: 1273 981 797
Fragments arranged by size:
1273 981 797
AflIII A'CryG_T
Cuts at: 0 334 1360 3051
Size: 334 1026 1691
Fragments arranged by size:
1691 1026 334
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1078 1110 3051
Size: 1078 32 1941
Fragments arranged by size:
1941 1078 32
ApoI r'AATT_y
Cuts at: 0 49 235 1268 1750 2754 3051
Size: 49 186 1033 482 1004 297
Fragments arranged by size:
1033 1004 482 297 186 49
BamHI G'GATC_C
Cuts at: 0 945 2916 3051
Size: 945 1971 135
Fragments arranged by size:
1971 945 135
BanII G_rGCy'C
Cuts at: 0 583 3008 3051
Size: 583 2425 43
Fragments arranged by size:
2425 583 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 701 808 2400 3051
Size: 701 107 1592 651
Fragments arranged by size:
1592 701 651 107
BciVI GTATCCnnnnn_n'
Cuts at: 0 377 1581 2559 3051
Size: 377 1204 978 492
Fragments arranged by size:
1204 978 492 377
BclI T'GATC_A
Cuts at: 0 723 3051
Size: 723 2328
BglII A'GATC_T
Cuts at: 0 2196 3051
Size: 2196 855
BlpI GC'TnA_GC
Cuts at: 0 2567 3051
Size: 2567 484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2221 3051
Size: 2221 830
BsaI GGTCTCn'nnnn_
Cuts at: 0 1680 3051
Size: 1680 1371
BsaAI yAC'GTr
Cuts at: 0 2611 3051
Size: 2611 440
BsaBI GATnn'nnATC
Cuts at: 0 1634 2603 3051
Size: 1634 969 448
Fragments arranged by size:
1634 969 448
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2944 2974 3051
Size: 2944 30 77
Fragments arranged by size:
2944 77 30
BseYI C'CCAG_C
Cuts at: 0 2552 3051
Size: 2552 499
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2657 3051
Size: 2657 394
BsiHKAI G_wGCw'C
Cuts at: 0 3008 3051
Size: 3008 43
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2480 3051
Size: 2480 571
Bsp1286I G_dGCh'C
Cuts at: 0 583 3008 3051
Size: 583 2425 43
Fragments arranged by size:
2425 583 43
BsrDI GCAATG_nn'
Cuts at: 0 301 1576 3051
Size: 301 1275 1475
Fragments arranged by size:
1475 1275 301
BsrFI r'CCGG_y
Cuts at: 0 1060 3051
Size: 1060 1991
BsrGI T'GTAC_A
Cuts at: 0 1371 2736 3051
Size: 1371 1365 315
Fragments arranged by size:
1371 1365 315
BstYI r'GATC_y
Cuts at: 0 945 1449 1544 2196 2832 2916 3051
Size: 945 504 95 652 636 84 135
Fragments arranged by size:
945 652 636 504 135 95 84
Bsu36I CC'TnA_GG
Cuts at: 0 100 3051
Size: 100 2951
BtgI C'CryG_G
Cuts at: 0 2506 3051
Size: 2506 545
BtsI GCAGTG_nn'
Cuts at: 0 91 2874 3051
Size: 91 2783 177
Fragments arranged by size:
2783 177 91
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 897 932 2810 2845 3051
Size: 897 35 1878 35 206
Fragments arranged by size:
1878 897 206 35 35
DraI TTT'AAA
Cuts at: 0 1601 3051
Size: 1601 1450
EaeI y'GGCC_r
Cuts at: 0 2024 3051
Size: 2024 1027
EarI CTCTTCn'nnn_
Cuts at: 0 359 1683 1908 3051
Size: 359 1324 225 1143
Fragments arranged by size:
1324 1143 359 225
EcoICRI GAG'CTC
Cuts at: 0 3006 3051
Size: 3006 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 980 1664 1997 2221 3051
Size: 713 267 684 333 224 830
Fragments arranged by size:
830 713 684 333 267 224
EcoO109I rG'GnC_Cy
Cuts at: 0 443 1243 3051
Size: 443 800 1808
Fragments arranged by size:
1808 800 443
EcoRI G'AATT_C
Cuts at: 0 1750 3051
Size: 1750 1301
FspI TGC'GCA
Cuts at: 0 629 3051
Size: 629 2422
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2136 2168 2263 2295 2993 3025 3051
Size: 2136 32 95 32 698 32 26
Fragments arranged by size:
2136 698 95 32 32 32 26
HincII GTy'rAC
Cuts at: 0 203 321 866 1028 2928 3051
Size: 203 118 545 162 1900 123
Fragments arranged by size:
1900 545 203 162 123 118
HindIII A'AGCT_T
Cuts at: 0 2904 3051
Size: 2904 147
HpaI GTT'AAC
Cuts at: 0 2928 3051
Size: 2928 123
MfeI C'AATT_G
Cuts at: 0 1217 3051
Size: 1217 1834
MspA1I CmG'CkG
Cuts at: 0 1210 3051
Size: 1210 1841
NcoI C'CATG_G
Cuts at: 0 2506 3051
Size: 2506 545
NspI r_CATG'y
Cuts at: 0 338 1364 3051
Size: 338 1026 1687
Fragments arranged by size:
1687 1026 338
PciI A'CATG_T
Cuts at: 0 334 1360 3051
Size: 334 1026 1691
Fragments arranged by size:
1691 1026 334
PfoI T'CCnGG_A
Cuts at: 0 796 3051
Size: 796 2255
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2403 2435 3051
Size: 2403 32 616
Fragments arranged by size:
2403 616 32
PpuMI rG'GwC_Cy
Cuts at: 0 443 1243 3051
Size: 443 800 1808
Fragments arranged by size:
1808 800 443
PshAI GACnn'nnGTC
Cuts at: 0 208 406 3051
Size: 208 198 2645
Fragments arranged by size:
2645 208 198
PstI C_TGCA'G
Cuts at: 0 2051 3051
Size: 2051 1000
PvuII CAG'CTG
Cuts at: 0 1210 3051
Size: 1210 1841
SacI G_AGCT'C
Cuts at: 0 3008 3051
Size: 3008 43
ScaI AGT'ACT
Cuts at: 0 1323 3051
Size: 1323 1728
SfcI C'TryA_G
Cuts at: 0 2047 3051
Size: 2047 1004
SmlI C'TyrA_G
Cuts at: 0 1273 2254 3051
Size: 1273 981 797
Fragments arranged by size:
1273 981 797
SnaBI TAC'GTA
Cuts at: 0 2611 3051
Size: 2611 440
SspI AAT'ATT
Cuts at: 0 763 844 3051
Size: 763 81 2207
Fragments arranged by size:
2207 763 81
StyI C'CwwG_G
Cuts at: 0 2506 3051
Size: 2506 545
TatI w'GTAC_w
Cuts at: 0 847 1321 1371 2736 3051
Size: 847 474 50 1365 315
Fragments arranged by size:
1365 847 474 315 50
Tth111I GACn'n_nGTC
Cuts at: 0 2625 3051
Size: 2625 426
XbaI T'CTAG_A
Cuts at: 0 120 3051
Size: 120 2931
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1104 2102 3051
Size: 1104 998 949
Fragments arranged by size:
1104 998 949
XmnI GAAnn'nnTTC
Cuts at: 0 353 2534 2996 3051
Size: 353 2181 462 55
Fragments arranged by size:
2181 462 353 55
Enzymes that do cut:
AcuI AflII AflIII AloI ApoI BamHI BanII BbsI
BciVI BclI BglII BlpI BpmI BsaI BsaAI BsaBI
BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrDI BsrFI
BsrGI BstYI Bsu36I BtgI BtsI CspCI DraI EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI FspI Hin4I HincII
HindIII HpaI MfeI MspA1I NcoI NspI PciI PfoI
PpiI PpuMI PshAI PstI PvuII SacI ScaI SfcI
SmlI SnaBI SspI StyI TatI Tth111I XbaI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbvCI BcgI BfrBI
BglI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaHI BsaWI BseRI BsiEI BsiWI BsmI BspEI BspHI
BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I BtgZI ClaI DraIII DrdI EagI EciI EcoNI
EcoRV FalI FseI FspAI HaeII KasI KpnI MluI
MmeI MscI MslI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SpeI SphI SrfI StuI SwaI TaqII TstI
XhoI XmaI ZraI