Restriction map for pF1KA1685
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AocI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BamHI       BbeI        BfrI        BlnI        BsaHI       
BseAI       BsiWI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraIII      Eam1105I    Eco81I      EcoO65I     EcoRV       
EcoT22I     FseI        FspI        Hin1I       HincII      
HindII      HpaI        KasI        KpnI        KspI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NotI        
NruI        NsiI        PacI        PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
Sse8387I    SspBI       SwaI        XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AflIII       1755  ACGTGT               A^C[GA][CT]GT
Aor51HI      2973  AGCGCT               AGC^GCT
BalI         4134  TGGCCA               TGG^CCA
BanI         2527  GGTGCC               G^G[CT][GA]CC
BbrPI        1754  CACGTG               CAC^GTG
BcgI         2750  CGAGCACTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2834  GTATCC               GTATCC(6/5)
BglI         2437  GCCACAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         754  AGATCT               A^GATCT
BsaAI        1754  CACGTG               [CT]AC^GT[GA]
BsaBI        1453  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         819  ACCGGA               [AT]^CCGG[AT]
BsiEI        3393  CGGCCG               CG[GA][CT]^CG
BsmBI        4284  CGTCTC               CGTCTC(1/5)
BsrBI        1628  GAGCGG               CCGCTC(-3/-3)
BstBI        4191  TTCGAA               TT^CGAA
BstXI        3606  CCAGAGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI         2796  TTTAAA               TTT^AAA
DrdI          935  GACAGGTTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         3393  CGGCCG               C^GGCCG
EclXI        3393  CGGCCG               C^GGCCG
Eco47III     2973  AGCGCT               AGC^GCT
Eco52I       3393  CGGCCG               C^GGCCG
EcoNI        2077  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI         4283  GCGTC                GACGC(5/10)
HindIII      2692  AAGCTT               A^AGCTT
MamI         1453  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        4134  TGGCCA               TGG^CCA
MscI         4134  TGGCCA               TGG^CCA
NspV         4191  TTCGAA               TT^CGAA
PaeR7I       2748  CTCGAG               C^TCGAG
PmaCI        1754  CACGTG               CAC^GTG
PmlI         1754  CACGTG               CAC^GTG
SacI         2566  GAGCTC               GAGCT^C
ScaI         3553  AGTACT               AGT^ACT
SexAI        4035  ACCAGGT              A^CC[AT]GGT
SfuI         4191  TTCGAA               TT^CGAA
SpeI         1939  ACTAGT               A^CTAGT
SspI         3379  AATATT               AAT^ATT
StuI         1247  AGGCCT               AGG^CCT
XhoI         2748  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

ApaI         2175  GGGCCC               GGGCC^C
             2467  GGGCCC              
AspI         2093  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2152  GACAGAGTC           
AvaI         2748  CTCGAG               C^[CT]CG[GA]G
             3856  CCCGAG              
BbsI         3292  GAAGAC               GAAGAC(2/6)
             1707  GTCTTC              
BbvCI        1397  CCTCAGC              CCTCAGC(-5/-2)
             3129  GCTGAGG             
BcnI         1863  CCCGG                CC^[GC]GG
             3396  CCGGG               
BpuAI        3292  GAAGAC               GAAGAC(2/6)
             1707  GTCTTC              
BsgI         1357  GTGCAG               GTGCAG(16/14)
             2546  GTGCAG              
BsmI          168  GAATGC               GAATGC(1/-1)
             1427  GCATTC              
BsoBI        2748  CTCGAG               C^[CT]CG[GA]G
             3856  CCCGAG              
EaeI         3393  CGGCCG               [CT]^GGCC[GA]
             4134  TGGCCA              
EcoRI         988  GAATTC               G^AATTC
             1064  GAATTC              
FauI          417  CCCGC                CCCGC(4/6)
             1630  GCGGG               
MslI         1233  CATGCCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             4218  CATGATCATG          
NciI         1863  CCCGG                CC^[GC]GG
             3396  CCGGG               
NheI         3679  GCTAGC               G^CTAGC
             4105  GCTAGC              
NspI          160  GCATGC               [GA]CATG^[CT]
             2362  GCATGC              
PflFI        2093  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2152  GACAGAGTC           
PflMI        2071  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2083  CCAGGGGGTGG         
PstI         2042  CTGCAG               CTGCA^G
             3890  CTGCAG              
SphI          160  GCATGC               GCATG^C
             2362  GCATGC              
Tth111I      2093  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2152  GACAGAGTC           
Van91I       2071  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2083  CCAGGGGGTGG         
XcmI         2180  CCACACTGGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3347  CCAAACCTGCAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AvaII       
BanII       BbvI        BclI        BfaI        BlpI        
BmyI        BpmI        Bpu1102I    BsaI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrDI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstYI       
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EarI        EcoO109I    
EcoRII      EcoT14I     FbaI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NcoI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com