Restriction map for pF1KA1685
 (Linear) (Six-base) MAPSORT of: KIAA1685.seq  Check: 9432  from: 1  to: 4305



KIAA1685.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     377    1047    2355    2736    4305 
   Size:        377     670    1308     381    1569

  Fragments arranged by size: 

                1569    1308     670     381     377
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     983    1364    2588    3091    3184    3458    3488    4305 
   Size:        983     381    1224     503      93     274      30     817

  Fragments arranged by size: 

                1224     983     817     503     381     274      93      30
            
 
AfeI AGC'GCT

Cuts at:      0    2975    4305 
   Size:       2975    1330
 
AflIII A'CryG_T

Cuts at:      0    1755    4305 
   Size:       1755    2550
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3085    4305 
   Size:       3085    1220
 
AlwNI CAG_nnn'CTG

Cuts at:      0      35    2020    2041    2077    2200    2512    2641    2728 
   Size:         35    1985      21      36     123     312     129      87

Cuts at:   2728    3057    4087    4305 
   Size:        329    1030     218

  Fragments arranged by size: 

                1985    1030     329     312     218     129     123      87
                  36      35      21
 
ApaI G_GGCC'C

Cuts at:      0    2179    2471    4305 
   Size:       2179     292    1834

  Fragments arranged by size: 

                2179    1834     292
 
ApoI r'AATT_y

Cuts at:      0      98     988    1064    2897    4305 
   Size:         98     890      76    1833    1408

  Fragments arranged by size: 

                1833    1408     890      98      76
 
AvaI C'yCGr_G

Cuts at:      0    2748    3856    4305 
   Size:       2748    1108     449

  Fragments arranged by size: 

                2748    1108     449
 
BanI G'GyrC_C

Cuts at:      0    2527    4305 
   Size:       2527    1778
 
BanII G_rGCy'C

Cuts at:      0    1388    1687    1920    2179    2471    2570    3403    3596 
   Size:       1388     299     233     259     292      99     833     193

Cuts at:   3596    4305 
   Size:        709

  Fragments arranged by size: 

                1388     833     709     299     292     259     233     193
                  99
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1700    3299    4305 
   Size:       1700    1599    1006

  Fragments arranged by size: 

                1700    1599    1006
 
BbvCI CC'TCA_GC

Cuts at:      0    1398    3130    4305 
   Size:       1398    1732    1175

  Fragments arranged by size: 

                1732    1398    1175
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2739    2773    4305 
   Size:       2739      34    1532

  Fragments arranged by size: 

                2739    1532      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2845    4305 
   Size:       2845    1460
 
BclI T'GATC_A

Cuts at:      0     857    1677    3989    4220    4305 
   Size:        857     820    2312     231      85

  Fragments arranged by size: 

                2312     857     820     231      85
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2443    4305 
   Size:       2443    1862
 
BglII A'GATC_T

Cuts at:      0     754    4305 
   Size:        754    3551
 
BlpI GC'TnA_GC

Cuts at:      0     972    1420    3675    4305 
   Size:        972     448    2255     630

  Fragments arranged by size: 

                2255     972     630     448
 
Bme1580I G_kGCm'C

Cuts at:      0    1447    2179    2471    3368    4186    4305 
   Size:       1447     732     292     897     818     119

  Fragments arranged by size: 

                1447     897     818     732     292     119
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1181    1275    2250    2326    2465    4305 
   Size:       1181      94     975      76     139    1840

  Fragments arranged by size: 

                1840    1181     975     139      94      76
 
BmtI G_CTAG'C

Cuts at:      0    3683    4109    4305 
   Size:       3683     426     196

  Fragments arranged by size: 

                3683     426     196
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     174     672     908    1854    2327    3123    4305 
   Size:        174     498     236     946     473     796    1182

  Fragments arranged by size: 

                1182     946     796     498     473     236     174
 
Bpu10I CC'TnA_GC

Cuts at:      0    1150    1398    3122    3130    4305 
   Size:       1150     248    1724       8    1175

  Fragments arranged by size: 

                1724    1175    1150     248       8
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1681    1840    4305 
   Size:       1681     159    2465

  Fragments arranged by size: 

                2465    1681     159
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1800    1872    1911    2257    4305 
   Size:       1800      72      39     346    2048

  Fragments arranged by size: 

                2048    1800     346      72      39
 
BsaAI yAC'GTr

Cuts at:      0    1756    4305 
   Size:       1756    2549
 
BsaBI GATnn'nnATC

Cuts at:      0    1457    4305 
   Size:       1457    2848
 
BsaWI w'CCGG_w

Cuts at:      0     819    4305 
   Size:        819    3486
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     172     202     670     700    1167    1197    4305 
   Size:        172      30     468      30     467      30    3108

  Fragments arranged by size: 

                3108     468     467     172      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     143     555    2147    3604    4305 
   Size:        143     412    1592    1457     701

  Fragments arranged by size: 

                1592    1457     701     412     143
 
BseYI C'CCAG_C

Cuts at:      0    1212    1739    3057    3321    4123    4305 
   Size:       1212     527    1318     264     802     182

  Fragments arranged by size: 

                1318    1212     802     527     264     182
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1378    2567    4305 
   Size:       1378    1189    1738

  Fragments arranged by size: 

                1738    1378    1189
 
BsiEI CG_ry'CG

Cuts at:      0    3396    4305 
   Size:       3396     909
 
BsiHKAI G_wGCw'C

Cuts at:      0     855    2313    2570    2755    4305 
   Size:        855    1458     257     185    1550

  Fragments arranged by size: 

                1550    1458     855     257     185
 
BsmI GAATG_Cn'

Cuts at:      0     174    1427    4305 
   Size:        174    1253    2878

  Fragments arranged by size: 

                2878    1253     174
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    4290    4305 
   Size:       4290      15
 
Bsp1286I G_dGCh'C

Cuts at:      0     855    1388    1447    1687    1920    2179    2313    2471 
   Size:        855     533      59     240     233     259     134     158

Cuts at:   2471    2570    2755    3368    3403    3596    4186    4305 
   Size:         99     185     613      35     193     590     119

  Fragments arranged by size: 

                 855     613     590     533     259     240     233     193
                 185     158     134     119      99      59      35
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     377     442     627     636    1047    1893    2355    2566 
   Size:        377      65     185       9     411     846     462     211

Cuts at:   2566    2736    3360    4305 
   Size:        170     624     945

  Fragments arranged by size: 

                 945     846     624     462     411     377     211     185
                 170      65       9
 
BsrBI CCG'CTC

Cuts at:      0    1630    4305 
   Size:       1630    2675
 
BsrDI GCAATG_nn'

Cuts at:      0     179     547    1303    3362    4062    4305 
   Size:        179     368     756    2059     700     243

  Fragments arranged by size: 

                2059     756     700     368     243     179
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     906    2020    2041    2901    4305 
   Size:        906    1114      21     860    1404

  Fragments arranged by size: 

                1404    1114     906     860      21
 
BstBI TT'CG_AA

Cuts at:      0    4192    4305 
   Size:       4192     113
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3613    4305 
   Size:       3613     692
 
BstYI r'GATC_y

Cuts at:      0     754    1458    1859    3194    4305 
   Size:        754     704     401    1335    1111

  Fragments arranged by size: 

                1335    1111     754     704     401
 
BtgI C'CryG_G

Cuts at:      0    1102    2888    4131    4305 
   Size:       1102    1786    1243     174

  Fragments arranged by size: 

                1786    1243    1102     174
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1553    1883    4305 
   Size:       1553     330    2422

  Fragments arranged by size: 

                2422    1553     330
 
BtsI GCAGTG_nn'

Cuts at:      0     309     357    3833    4305 
   Size:        309      48    3476     472

  Fragments arranged by size: 

                3476     472     309      48
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    4218    4253    4305 
   Size:       4218      35      52

  Fragments arranged by size: 

                4218      52      35
 
DraI TTT'AAA

Cuts at:      0    2798    4305 
   Size:       2798    1507
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     941    4305 
   Size:        941    3364
 
EaeI y'GGCC_r

Cuts at:      0    3393    4134    4305 
   Size:       3393     741     171

  Fragments arranged by size: 

                3393     741     171
 
EagI C'GGCC_G

Cuts at:      0    3393    4305 
   Size:       3393     912
 
EarI CTCTTCn'nnn_

Cuts at:      0     194    1328    1718    1741    4160    4305 
   Size:        194    1134     390      23    2419     145

  Fragments arranged by size: 

                2419    1134     390     194     145      23
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      55    4305 
   Size:         55    4250
 
EcoICRI GAG'CTC

Cuts at:      0    2568    4305 
   Size:       2568    1737
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     174     672     908     983    1364    1854    2327    2588 
   Size:        174     498     236      75     381     490     473     261

Cuts at:   2588    3091    3123    3184    3458    3488    4305 
   Size:        503      32      61     274      30     817

  Fragments arranged by size: 

                 817     503     498     490     473     381     274     261
                 236     174      75      61      32      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2081    4305 
   Size:       2081    2224
 
EcoO109I rG'GnC_Cy

Cuts at:      0      32    1728    2074    2175    2176    2467    2509    2578 
   Size:         32    1696     346     101       1     291      42      69

Cuts at:   2578    3101    3419    3637    4305 
   Size:        523     318     218     668

  Fragments arranged by size: 

                1696     668     523     346     318     291     218     101
                  69      42      32       1
 
EcoRI G'AATT_C

Cuts at:      0     988    1064    4305 
   Size:        988      76    3241

  Fragments arranged by size: 

                3241     988      76
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      75     118     164    4305 
   Size:         75      43      46    4141

  Fragments arranged by size: 

                4141      75      46      43
 
HaeII r_GCGC'y

Cuts at:      0     508    2977    4244    4305 
   Size:        508    2469    1267      61

  Fragments arranged by size: 

                2469    1267     508      61
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     138     170     298     330     842     874    1167    1199 
   Size:        138      32     128      32     512      32     293      32

Cuts at:   1199    3016    3048    3216    3248    3556    3588    3974    4006 
   Size:       1817      32     168      32     308      32     386      32

Cuts at:   4006    4009    4041    4057    4089    4305 
   Size:          3      32      16      32     216

  Fragments arranged by size: 

                1817     512     386     308     293     216     168     138
                 128      32      32      32      32      32      32      32
                  32      32      32      16       3
 
HindIII A'AGCT_T

Cuts at:      0    2692    4305 
   Size:       2692    1613
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     593    2037    2365    2527    4305 
   Size:        593    1444     328     162    1778

  Fragments arranged by size: 

                1778    1444     593     328     162
 
MscI TGG'CCA

Cuts at:      0    4136    4305 
   Size:       4136     169
 
MslI CAynn'nnrTG

Cuts at:      0    1237    4222    4305 
   Size:       1237    2985      83

  Fragments arranged by size: 

                2985    1237      83
 
MspA1I CmG'CkG

Cuts at:      0    1216    2020    2026    2038    2551    2865    3057    3288 
   Size:       1216     804       6      12     513     314     192     231

Cuts at:   3288    4305 
   Size:       1017

  Fragments arranged by size: 

                1216    1017     804     513     314     231     192      12
                   6
 
NcoI C'CATG_G

Cuts at:      0    1102    2888    4131    4305 
   Size:       1102    1786    1243     174

  Fragments arranged by size: 

                1786    1243    1102     174
 
NheI G'CTAG_C

Cuts at:      0    3679    4105    4305 
   Size:       3679     426     200

  Fragments arranged by size: 

                3679     426     200
 
NspI r_CATG'y

Cuts at:      0     164    2366    4305 
   Size:        164    2202    1939

  Fragments arranged by size: 

                2202    1939     164
 
PasI CC'CwG_GG

Cuts at:      0    1913    2083    4305 
   Size:       1913     170    2222

  Fragments arranged by size: 

                2222    1913     170
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2077    2089    4305 
   Size:       2077      12    2216

  Fragments arranged by size: 

                2216    2077      12
 
PfoI T'CCnGG_A

Cuts at:      0     885    2070    2100    4305 
   Size:        885    1185      30    2205

  Fragments arranged by size: 

                2205    1185     885      30
 
PmlI CAC'GTG

Cuts at:      0    1756    4305 
   Size:       1756    2549
 
PpuMI rG'GwC_Cy

Cuts at:      0      32    2074    2509    2578    3419    3637    4305 
   Size:         32    2042     435      69     841     218     668

  Fragments arranged by size: 

                2042     841     668     435     218      69      32
 
PsiI TTA'TAA

Cuts at:      0    1535    4305 
   Size:       1535    2770
 
PspOMI G'GGCC_C

Cuts at:      0    2175    2467    4305 
   Size:       2175     292    1838

  Fragments arranged by size: 

                2175    1838     292
 
PspXI vC'TCGA_Gb

Cuts at:      0    2748    4305 
   Size:       2748    1557
 
PstI C_TGCA'G

Cuts at:      0    2046    3894    4305 
   Size:       2046    1848     411

  Fragments arranged by size: 

                2046    1848     411
 
PvuII CAG'CTG

Cuts at:      0    1216    2020    2038    2551    2865    3057    3288    4305 
   Size:       1216     804      18     513     314     192     231    1017

  Fragments arranged by size: 

                1216    1017     804     513     314     231     192      18
            
 
SacI G_AGCT'C

Cuts at:      0    2570    4305 
   Size:       2570    1735
 
ScaI AGT'ACT

Cuts at:      0    3555    4305 
   Size:       3555     750
 
SexAI A'CCwGG_T

Cuts at:      0    4035    4305 
   Size:       4035     270
 
SfcI C'TryA_G

Cuts at:      0    2042    2317    2720    3890    3978    4305 
   Size:       2042     275     403    1170      88     327

  Fragments arranged by size: 

                2042    1170     403     327     275      88
 
SmlI C'TyrA_G

Cuts at:      0    1696    1855    2748    4305 
   Size:       1696     159     893    1557

  Fragments arranged by size: 

                1696    1557     893     159
 
SpeI A'CTAG_T

Cuts at:      0    1939    4305 
   Size:       1939    2366
 
SphI G_CATG'C

Cuts at:      0     164    2366    4305 
   Size:        164    2202    1939

  Fragments arranged by size: 

                2202    1939     164
 
SspI AAT'ATT

Cuts at:      0    3381    4305 
   Size:       3381     924
 
StuI AGG'CCT

Cuts at:      0    1249    4305 
   Size:       1249    3056
 
StyI C'CwwG_G

Cuts at:      0    1008    1102    1347    2888    3097    4131    4305 
   Size:       1008      94     245    1541     209    1034     174

  Fragments arranged by size: 

                1541    1034    1008     245     209     174      94
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      96     453    1732    3919    3976    4305 
   Size:         96     357    1279    2187      57     329

  Fragments arranged by size: 

                2187    1279     357     329      96      57
 
TatI w'GTAC_w

Cuts at:      0    3553    4305 
   Size:       3553     752
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     172     204    4305 
   Size:        172      32    4101

  Fragments arranged by size: 

                4101     172      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2096    2155    4305 
   Size:       2096      59    2150

  Fragments arranged by size: 

                2150    2096      59
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2187    3354    4305 
   Size:       2187    1167     951

  Fragments arranged by size: 

                2187    1167     951
 
XhoI C'TCGA_G

Cuts at:      0    2748    4305 
   Size:       2748    1557
 
XmnI GAAnn'nnTTC

Cuts at:      0     101     745    2693    4305 
   Size:        101     644    1948    1612

  Fragments arranged by size: 

                1948    1612     644     101
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AlfI    AlwNI     ApaI     ApoI
     AvaI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrDI   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I  HindIII
     MmeI     MscI     MslI   MspA1I     NcoI     NheI     NspI     PasI
    PflMI     PfoI     PmlI    PpuMI     PsiI   PspOMI    PspXI     PstI
    PvuII     SacI     ScaI    SexAI     SfcI     SmlI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BfrBI    BmgBI     BplI    BsaHI    BsiWI    BspEI    BspHI
    BsrFI    BsrGI   BssHII    BssSI   BstEII  BstZ17I   Bsu36I     ClaI
   DraIII    EcoRV     FseI     FspI    FspAI   HincII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NotI
     NruI     NsiI     PacI     PciI     PmeI     PpiI    PshAI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI     SwaI     XbaI
     XmaI     ZraI
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