Restriction map for pF1KA1682
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflIII      AgeI        AhdI        
AscI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       BamHI       BbeI        BbrPI       
BcgI        BglII       BlpI        Bpu1102I    BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
EagI        Eam1105I    EclXI       Eco52I      EcoNI       
EcoO65I     EcoRV       FseI        FspI        HgaI        
Hin1I       HpaI        KasI        KpnI        KspI        
MamI        MfeI        MflI        MluI        MroI        
MslI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspI        NspV        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SpeI        
SphI        SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

AflII         622  CTTAAG               C^TTAAG
Alw44I       1387  GTGCAC               G^TGCAC
AocI           76  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1387  GTGCAC               G^TGCAC
AseI         1795  ATTAAT               AT^TAAT
AsnI         1795  ATTAAT               AT^TAAT
BanI           34  GGCACC               G^G[CT][GA]CC
BciVI        1527  GGATAC               GTATCC(6/5)
BclI          342  TGATCA               T^GATCA
BcnI         1401  CCCGG                CC^[GC]GG
BfrI          622  CTTAAG               C^TTAAG
BglI         2059  GCCCCCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI         2131  CTCCAG               CTGGAG(16/14)
BsaAI          67  TACGTA               [CT]AC^GT[GA]
BsrDI        1601  CATTGC               GCAATG(2/0)
BstAPI       1380  GCAGCTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I         76  CCTGAGG              CC^T[ACGT]AGG
DraI         1000  TTTAAA               TTT^AAA
DraIII       1557  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1431  GACTGTTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1869  CCATGG               C^C[GA][CT]GG
Eco81I         76  CCTGAGG              CC^T[ACGT]AGG
EcoRI        1411  GAATTC               G^AATTC
EcoT22I      1178  ATGCAT               ATGCA^T
FauI         2099  CCCGC                CCCGC(4/6)
FbaI          342  TGATCA               T^GATCA
HapII        1402  CCGG                 C^CGG
HincII        685  GTCAAC               GT[CT]^[GA]AC
HindII        685  GTCAAC               GT[CT]^[GA]AC
HindIII      2177  AAGCTT               A^AGCTT
HpaII        1402  CCGG                 C^CGG
MspI         1402  CCGG                 C^CGG
NciI         1401  CCCGG                CC^[GC]GG
NcoI         1869  CCATGG               C^CATGG
NsiI         1178  ATGCAT               ATGCA^T
PacI         1756  TTAATTAA             TTAAT^TAA
PflMI        2165  CCAGTGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        1795  ATTAAT               AT^TAAT
SacI         1810  GAGCTC               GAGCT^C
SbfI          969  CCTGCAGG             CCTGCA^GG
SmlI          622  CTTAAG               C^T[CT][GA]AG
SnaBI          67  TACGTA               TAC^GTA
Sse8387I      969  CCTGCAGG             CCTGCA^GG
Tsp45I        583  GTCAC                ^GT[GC]AC
Van91I       2165  CCAGTGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwI          589  GATCC                GGATC(4/5)
             1305  GGATC               
AlwNI         581  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2166  CAGTGGCTG           
Aor51HI       764  AGCGCT               AGC^GCT
             1922  AGCGCT              
ApaI         1897  GGGCCC               GGGCC^C
             1916  GGGCCC              
AspHI        1387  GTGCAC               G[AT]GC[AT]^C
             1810  GAGCTC              
AvrII        1956  CCTAGG               C^CTAGG
             2229  CCTAGG              
BbsI          943  GAAGAC               GAAGAC(2/6)
             2191  GAAGAC              
BbvCI        2068  GCTGAGG              CCTCAGC(-5/-2)
             2085  GCTGAGG             
BlnI         1956  CCTAGG               C^CTAGG
             2229  CCTAGG              
BpuAI         943  GAAGAC               GAAGAC(2/6)
             2191  GAAGAC              
BseRI        1171  GAGGAG               GAGGAG(10/8)
             1339  GAGGAG              
BsiHKAI      1387  GTGCAC               G[AT]GC[AT]^C
             1810  GAGCTC              
BsiWI          65  CGTACG               C^GTACG
               69  CGTACG              
BsmI         1176  GAATGC               GAATGC(1/-1)
             1661  GAATGC              
Eco47III      764  AGCGCT               AGC^GCT
             1922  AGCGCT              
HaeII         764  AGCGCT               [GA]GCGC^[CT]
             1922  AGCGCT              
PstI          970  CTGCAG               CTGCA^G
             1236  CTGCAG              
ScaI          171  AGTACT               AGT^ACT
             1027  AGTACT              
SfaNI        1180  GCATC                GCATC(5/9)
             1395  GCATC               
SfcI          970  CTGCAG               C^T[GA][CT]AG
             1236  CTGCAG              
XcmI          787  CCAATATGGTGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1950  CCAAATCCTAGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaII       BalI        BanII       BbvI        BfaI        
BmyI        BsaJI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HhaI        HinP1I      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MseI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       TthHB8I     
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