Restriction map for pF1KA1682
 (Linear) (Six-base) MAPSORT of: KIAA1682.seq  Check: 5148  from: 1  to: 2241



KIAA1682.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     415     770    2241 
   Size:        196     219     355    1471

  Fragments arranged by size: 

                1471     355     219     196
 
AfeI AGC'GCT

Cuts at:      0     766    1924    2241 
   Size:        766    1158     317

  Fragments arranged by size: 

                1158     766     317
 
AflII C'TTAA_G

Cuts at:      0     622    2241 
   Size:        622    1619
 
AlwNI CAG_nnn'CTG

Cuts at:      0     586    2171    2241 
   Size:        586    1585      70

  Fragments arranged by size: 

                1585     586      70
 
ApaI G_GGCC'C

Cuts at:      0    1901    1920    2241 
   Size:       1901      19     321

  Fragments arranged by size: 

                1901     321      19
 
ApaLI G'TGCA_C

Cuts at:      0    1387    2241 
   Size:       1387     854
 
ApoI r'AATT_y

Cuts at:      0      83     896     997    1033    1411    2241 
   Size:         83     813     101      36     378     830

  Fragments arranged by size: 

                 830     813     378     101      83      36
 
AseI AT'TA_AT

Cuts at:      0    1796    2241 
   Size:       1796     445
 
AvrII C'CTAG_G

Cuts at:      0    1956    2229    2241 
   Size:       1956     273      12

  Fragments arranged by size: 

                1956     273      12
 
BanI G'GyrC_C

Cuts at:      0      34    2241 
   Size:         34    2207
 
BanII G_rGCy'C

Cuts at:      0    1814    1901    1920    2241 
   Size:       1814      87      19     321

  Fragments arranged by size: 

                1814     321      87      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     950    2198    2241 
   Size:        950    1248      43

  Fragments arranged by size: 

                1248     950      43
 
BbvCI CC'TCA_GC

Cuts at:      0    2069    2086    2241 
   Size:       2069      17     155

  Fragments arranged by size: 

                2069     155      17
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1521    2241 
   Size:       1521     720
 
BclI T'GATC_A

Cuts at:      0     342    2241 
   Size:        342    1899
 
BfrBI ATG'CAT

Cuts at:      0    1180    2241 
   Size:       1180    1061
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2065    2241 
   Size:       2065     176
 
Bme1580I G_kGCm'C

Cuts at:      0     822    1391    1901    1920    2241 
   Size:        822     569     510      19     321

  Fragments arranged by size: 

                 822     569     510     321      19
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2116    2241 
   Size:       2116     125
 
Bpu10I CC'TnA_GC

Cuts at:      0    2069    2086    2241 
   Size:       2069      17     155

  Fragments arranged by size: 

                2069     155      17
 
BsaAI yAC'GTr

Cuts at:      0      69    2241 
   Size:         69    2172
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     504     534    2241 
   Size:        504      30    1707

  Fragments arranged by size: 

                1707     504      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1186    1354    2241 
   Size:       1186     168     887

  Fragments arranged by size: 

                1186     887     168
 
BseYI C'CCAG_C

Cuts at:      0       3    1919    2241 
   Size:          3    1916     322

  Fragments arranged by size: 

                1916     322       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     551     781    1597    2241 
   Size:        213     338     230     816     644

  Fragments arranged by size: 

                 816     644     338     230     213
 
BsiHKAI G_wGCw'C

Cuts at:      0    1391    1814    2241 
   Size:       1391     423     427

  Fragments arranged by size: 

                1391     427     423
 
BsiWI C'GTAC_G

Cuts at:      0      65      69    2241 
   Size:         65       4    2172

  Fragments arranged by size: 

                2172      65       4
 
BsmI GAATG_Cn'

Cuts at:      0    1182    1667    2241 
   Size:       1182     485     574

  Fragments arranged by size: 

                1182     574     485
 
Bsp1286I G_dGCh'C

Cuts at:      0     822    1391    1814    1901    1920    2241 
   Size:        822     569     423      87      19     321

  Fragments arranged by size: 

                 822     569     423     321      87      19
 
BsrDI GCAATG_nn'

Cuts at:      0    1600    2241 
   Size:       1600     641
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1386    2241 
   Size:       1386     855
 
Bsu36I CC'TnA_GG

Cuts at:      0      77    2241 
   Size:         77    2164
 
BtgI C'CryG_G

Cuts at:      0    1869    2241 
   Size:       1869     372
 
BtsI GCAGTG_nn'

Cuts at:      0     423     683     838    1233    2241 
   Size:        423     260     155     395    1008

  Fragments arranged by size: 

                1008     423     395     260     155
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1258    1293    2241 
   Size:       1258      35     948

  Fragments arranged by size: 

                1258     948      35
 
DraI TTT'AAA

Cuts at:      0    1002    2241 
   Size:       1002    1239
 
DraIII CAC_nnn'GTG

Cuts at:      0    1562    2241 
   Size:       1562     679
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1437    2241 
   Size:       1437     804
 
EaeI y'GGCC_r

Cuts at:      0    1290    1965    1973    2201    2241 
   Size:       1290     675       8     228      40

  Fragments arranged by size: 

                1290     675     228      40       8
 
EarI CTCTTCn'nnn_

Cuts at:      0     131     467     746     749    2241 
   Size:        131     336     279       3    1492

  Fragments arranged by size: 

                1492     336     279     131       3
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1312    2241 
   Size:       1312     929
 
EcoICRI GAG'CTC

Cuts at:      0    1812    2241 
   Size:       1812     429
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     415     770    2116    2241 
   Size:        196     219     355    1346     125

  Fragments arranged by size: 

                1346     355     219     196     125
 
EcoO109I rG'GnC_Cy

Cuts at:      0      42    1897    2058    2073    2241 
   Size:         42    1855     161      15     168

  Fragments arranged by size: 

                1855     168     161      42      15
 
EcoRI G'AATT_C

Cuts at:      0    1411    2241 
   Size:       1411     830
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      72     142     264     903    1559    2172    2241 
   Size:         72      70     122     639     656     613      69

  Fragments arranged by size: 

                 656     639     613     122      72      70      69
 
HaeII r_GCGC'y

Cuts at:      0     768    1926    2241 
   Size:        768    1158     315

  Fragments arranged by size: 

                1158     768     315
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     250     282    1165    1197    2241 
   Size:        250      32     883      32    1044

  Fragments arranged by size: 

                1044     883     250      32      32
 
HincII GTy'rAC

Cuts at:      0     687    2241 
   Size:        687    1554
 
HindIII A'AGCT_T

Cuts at:      0    2177    2241 
   Size:       2177      64
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1049    1208    1915    2241 
   Size:       1049     159     707     326

  Fragments arranged by size: 

                1049     707     326     159
 
MscI TGG'CCA

Cuts at:      0    1292    1967    1975    2203    2241 
   Size:       1292     675       8     228      38

  Fragments arranged by size: 

                1292     675     228      38       8
 
MspA1I CmG'CkG

Cuts at:      0     150    1082    1383    1844    2241 
   Size:        150     932     301     461     397

  Fragments arranged by size: 

                 932     461     397     301     150
 
NcoI C'CATG_G

Cuts at:      0    1869    2241 
   Size:       1869     372
 
NsiI A_TGCA'T

Cuts at:      0    1182    2241 
   Size:       1182    1059
 
PacI TTA_AT'TAA

Cuts at:      0    1760    2241 
   Size:       1760     481
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2171    2241 
   Size:       2171      70
 
PfoI T'CCnGG_A

Cuts at:      0     185    2241 
   Size:        185    2056
 
PspOMI G'GGCC_C

Cuts at:      0    1897    1916    2241 
   Size:       1897      19     325

  Fragments arranged by size: 

                1897     325      19
 
PstI C_TGCA'G

Cuts at:      0     974    1240    2241 
   Size:        974     266    1001

  Fragments arranged by size: 

                1001     974     266
 
PvuII CAG'CTG

Cuts at:      0     150    1082    1383    1844    2241 
   Size:        150     932     301     461     397

  Fragments arranged by size: 

                 932     461     397     301     150
 
SacI G_AGCT'C

Cuts at:      0    1814    2241 
   Size:       1814     427
 
SbfI CC_TGCA'GG

Cuts at:      0     974    2241 
   Size:        974    1267
 
ScaI AGT'ACT

Cuts at:      0     173    1029    2241 
   Size:        173     856    1212

  Fragments arranged by size: 

                1212     856     173
 
SfcI C'TryA_G

Cuts at:      0     970    1236    2241 
   Size:        970     266    1005

  Fragments arranged by size: 

                1005     970     266
 
SmlI C'TyrA_G

Cuts at:      0     622    2241 
   Size:        622    1619
 
SnaBI TAC'GTA

Cuts at:      0      69    2241 
   Size:         69    2172
 
StyI C'CwwG_G

Cuts at:      0      38    1869    1956    2229    2241 
   Size:         38    1831      87     273      12

  Fragments arranged by size: 

                1831     273      87      38      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     244    1276    2241 
   Size:        244    1032     965

  Fragments arranged by size: 

                1032     965     244
 
TatI w'GTAC_w

Cuts at:      0     171     668    1027    2241 
   Size:        171     497     359    1214

  Fragments arranged by size: 

                1214     497     359     171
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     480     512     729     761    2241 
   Size:        480      32     217      32    1480

  Fragments arranged by size: 

                1480     480     217      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     794    1957    2241 
   Size:        794    1163     284

  Fragments arranged by size: 

                1163     794     284
 

 Enzymes that do cut:

     AcuI     AfeI    AflII    AlwNI     ApaI    ApaLI     ApoI     AseI
    AvrII     BanI    BanII     BbsI    BbvCI    BciVI     BclI    BfrBI
     BglI Bme1580I     BpmI   Bpu10I    BsaAI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsiWI     BsmI Bsp1286I    BsrDI   BstAPI   Bsu36I
     BtgI     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I
   HincII  HindIII     MmeI     MscI   MspA1I     NcoI     NsiI     PacI
    PflMI     PfoI   PspOMI     PstI    PvuII     SacI     SbfI     ScaI
     SfcI     SmlI    SnaBI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     AscI    AsiSI     AvaI     BaeI    BamHI
     BbeI     BcgI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsiEI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I    BtgZI     ClaI     EagI    EcoNI
    EcoRV     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI
     MluI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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