Restriction map for pF1KA1649
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BpmI        BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BseAI       
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        DraIII      EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRV       FbaI        FseI        
FspI        HaeII       HgaI        Hin1I       HpaI        
KasI        KpnI        KspI        MaeI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        PacI        
PflFI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccII          37  CGCG                 CG^CG
AfaI          999  GTAC                 GT^AC
AflIII        755  ACATGT               A^C[GA][CT]GT
AgeI         1007  ACCGGT               A^CCGGT
ApaI          911  GGGCCC               GGGCC^C
AspHI         923  GAGCTC               G[AT]GC[AT]^C
BamHI         104  GGATCC               G^GATCC
BsaWI        1007  ACCGGT               [AT]^CCGG[AT]
BsiHKAI       923  GAGCTC               G[AT]GC[AT]^C
BspLU11I      755  ACATGT               A^CATGT
BsrFI        1007  ACCGGT               [GA]^CCGG[CT]
BstUI          37  CGCG                 CG^CG
BstXI         627  CCACATGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         104  GGATCC               [GA]^GATC[CT]
CfoI           36  GCGC                 GCG^C
Cfr10I       1007  ACCGGT               [GA]^CCGG[CT]
DrdI          750  GACCAACATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        1246  GAATTC               G^AATTC
EcoT22I       701  ATGCAT               ATGCA^T
HhaI           36  GCGC                 GCG^C
HinP1I         36  GCGC                 G^CGC
HincII        129  GTCAAC               GT[CT]^[GA]AC
HindII        129  GTCAAC               GT[CT]^[GA]AC
MflI          104  GGATCC               [GA]^GATC[CT]
MseI           62  TTAA                 T^TAA
MspA1I        803  CAGCTG               C[AC]G^C[GT]G
MvnI           37  CGCG                 CG^CG
NcoI          467  CCATGG               C^CATGG
NsiI          701  ATGCAT               ATGCA^T
NspI          755  ACATGT               [GA]CATG^[CT]
PinAI        1007  ACCGGT               A^CCGGT
PvuII         803  CAGCTG               CAG^CTG
RsaI          999  GTAC                 GT^AC
SacI          923  GAGCTC               GAGCT^C
StuI          114  AGGCCT               AGG^CCT
Tru9I          62  TTAA                 T^TAA
XcmI          624  CCACCACATGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         104  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcsI          537  GAATTT               [GA]^AATT[CT]
             1246  GAATTC              
AlwI          105  GATCC                GGATC(4/5)
              104  GGATC               
AlwNI         710  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              803  CAGCTGCTG           
AocI          608  CCTCAGG              CC^T[ACGT]AGG
             1214  CCTCAGG             
ApoI          537  GAATTT               [GA]^AATT[CT]
             1246  GAATTC              
AvaII         933  GGTCC                G^G[AT]CC
             1179  GGACC               
BanII         911  GGGCCC               G[GA]GC[CT]^C
              923  GAGCTC              
BlpI          639  GCTAAGC              GC^T[ACGT]AGC
              808  GCTGAGC             
Bpu1102I      639  GCTAAGC              GC^T[ACGT]AGC
              808  GCTGAGC             
BsgI          859  CTGCAC               GTGCAG(16/14)
              271  GTGCAG              
BsmFI         409  GGGAC                GGGAC(10/14)
              759  GTCCC               
BstBI         254  TTCGAA               TT^CGAA
              734  TTCGAA              
Bsu36I        608  CCTCAGG              CC^T[ACGT]AGG
             1214  CCTCAGG             
CelII         639  GCTAAGC              GC^T[ACGT]AGC
              808  GCTGAGC             
DsaI          314  CCACGG               C^C[GA][CT]GG
              467  CCATGG              
EarI         1204  CTCTTC               CTCTTC(1/4)
              369  GAAGAG              
Eco81I        608  CCTCAGG              CC^T[ACGT]AGG
             1214  CCTCAGG             
EcoT14I       467  CCATGG               C^C[AT][AT]GG
              665  CCAAGG              
FauI          332  CCCGC                CCCGC(4/6)
               38  GCGGG               
Ksp632I      1204  CTCTTC               CTCTTC(1/4)
              369  GAAGAG              
NspV          254  TTCGAA               TT^CGAA
              734  TTCGAA              
PaeR7I         95  CTCGAG               C^TCGAG
              866  CTCGAG              
PflMI         400  CCACCCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              624  CCACCACATGG         
PleI          209  GAGTC                GAGTC(4/5)
              869  GAGTC               
SfuI          254  TTCGAA               TT^CGAA
              734  TTCGAA              
SmaI          221  CCCGGG               CCC^GGG
              350  CCCGGG              
StyI          467  CCATGG               C^C[AT][AT]GG
              665  CCAAGG              
TfiI          257  GAATC                G^A[AT]TC
              503  GAATC               
Tsp509I       538  AATT                 ^AATT
             1247  AATT                
Van91I        400  CCACCCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              624  CCACCACATGG         
XhoI           95  CTCGAG               C^TCGAG
              866  CTCGAG              
XmaCI         221  CCCGGG               C^CCGGG
              350  CCCGGG              
XmaI          221  CCCGGG               C^CCGGG
              350  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AluI        AvaI        BanI        BbvI        
BcnI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HindIII     HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MslI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
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