Restriction map for pF1KA1649
 (Linear) (Six-base) MAPSORT of: KIAA1649.seq  Check: 958  from: 1  to: 1263



KIAA1649.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448    1216    1263 
   Size:        448     768      47

  Fragments arranged by size: 

                 768     448      47
 
AflIII A'CryG_T

Cuts at:      0     755    1263 
   Size:        755     508
 
AgeI A'CCGG_T

Cuts at:      0    1007    1263 
   Size:       1007     256
 
AleI CACnn'nnGTG

Cuts at:      0     685    1263 
   Size:        685     578
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     260    1263 
   Size:        260    1003
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1135    1167    1263 
   Size:       1135      32      96

  Fragments arranged by size: 

                1135      96      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     715     808    1263 
   Size:        715      93     455

  Fragments arranged by size: 

                 715     455      93
 
ApaI G_GGCC'C

Cuts at:      0     915    1263 
   Size:        915     348
 
ApoI r'AATT_y

Cuts at:      0     537    1246    1263 
   Size:        537     709      17

  Fragments arranged by size: 

                 709     537      17
 
AvaI C'yCGr_G

Cuts at:      0      95     221     350     866    1263 
   Size:         95     126     129     516     397

  Fragments arranged by size: 

                 516     397     129     126      95
 
BamHI G'GATC_C

Cuts at:      0     104    1263 
   Size:        104    1159
 
BanI G'GyrC_C

Cuts at:      0     318     471     835    1263 
   Size:        318     153     364     428

  Fragments arranged by size: 

                 428     364     318     153
 
BanII G_rGCy'C

Cuts at:      0     915     927    1263 
   Size:        915      12     336

  Fragments arranged by size: 

                 915     336      12
 
BfrBI ATG'CAT

Cuts at:      0     703    1263 
   Size:        703     560
 
BlpI GC'TnA_GC

Cuts at:      0     640     809    1263 
   Size:        640     169     454

  Fragments arranged by size: 

                 640     454     169
 
Bme1580I G_kGCm'C

Cuts at:      0     323     915    1263 
   Size:        323     592     348

  Fragments arranged by size: 

                 592     348     323
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     214    1263 
   Size:        214    1049
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     901    1263 
   Size:        901     362
 
BsaWI w'CCGG_w

Cuts at:      0    1007    1263 
   Size:       1007     256
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     943     973    1135    1165    1263 
   Size:        943      30     162      30      98

  Fragments arranged by size: 

                 943     162      98      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     387     892    1143    1146    1263 
   Size:        387     505     251       3     117

  Fragments arranged by size: 

                 505     387     251     117       3
 
BseYI C'CCAG_C

Cuts at:      0     634    1263 
   Size:        634     629
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     844    1263 
   Size:        292     552     419

  Fragments arranged by size: 

                 552     419     292
 
BsiHKAI G_wGCw'C

Cuts at:      0     927    1263 
   Size:        927     336
 
Bsp1286I G_dGCh'C

Cuts at:      0     323     915     927    1263 
   Size:        323     592      12     336

  Fragments arranged by size: 

                 592     336     323      12
 
BsrFI r'CCGG_y

Cuts at:      0    1007    1263 
   Size:       1007     256
 
BstBI TT'CG_AA

Cuts at:      0     255     735    1263 
   Size:        255     480     528

  Fragments arranged by size: 

                 528     480     255
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     634    1263 
   Size:        634     629
 
BstYI r'GATC_y

Cuts at:      0     104    1263 
   Size:        104    1159
 
Bsu36I CC'TnA_GG

Cuts at:      0     609    1215    1263 
   Size:        609     606      48

  Fragments arranged by size: 

                 609     606      48
 
BtgI C'CryG_G

Cuts at:      0     314     467    1263 
   Size:        314     153     796

  Fragments arranged by size: 

                 796     314     153
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     756    1263 
   Size:        756     507
 
EarI CTCTTCn'nnn_

Cuts at:      0     364    1210    1263 
   Size:        364     846      53

  Fragments arranged by size: 

                 846     364      53
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1263 
   Size:         19    1244
 
EcoICRI GAG'CTC

Cuts at:      0     925    1263 
   Size:        925     338
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448    1216    1263 
   Size:        448     768      47

  Fragments arranged by size: 

                 768     448      47
 
EcoO109I rG'GnC_Cy

Cuts at:      0     669     911     912    1220    1263 
   Size:        669     242       1     308      43

  Fragments arranged by size: 

                 669     308     242      43       1
 
EcoRI G'AATT_C

Cuts at:      0    1246    1263 
   Size:       1246      17
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      76     381    1221    1263 
   Size:         76     305     840      42

  Fragments arranged by size: 

                 840     305      76      42
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      89     121     242     274     408     440    1090    1122 
   Size:         89      32     121      32     134      32     650      32

Cuts at:   1122    1263 
   Size:        141

  Fragments arranged by size: 

                 650     141     134     121      89      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     131    1263 
   Size:        131    1132
 
HindIII A'AGCT_T

Cuts at:      0     229     652    1258    1263 
   Size:        229     423     606       5

  Fragments arranged by size: 

                 606     423     229       5
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      60    1184    1263 
   Size:         60    1124      79

  Fragments arranged by size: 

                1124      79      60
 
MslI CAynn'nnrTG

Cuts at:      0     685     841    1049    1263 
   Size:        685     156     208     214

  Fragments arranged by size: 

                 685     214     208     156
 
MspA1I CmG'CkG

Cuts at:      0     805    1263 
   Size:        805     458
 
NcoI C'CATG_G

Cuts at:      0     467    1263 
   Size:        467     796
 
NsiI A_TGCA'T

Cuts at:      0     705    1263 
   Size:        705     558
 
NspI r_CATG'y

Cuts at:      0     759    1263 
   Size:        759     504
 
PciI A'CATG_T

Cuts at:      0     755    1263 
   Size:        755     508
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     406     630    1263 
   Size:        406     224     633

  Fragments arranged by size: 

                 633     406     224
 
PfoI T'CCnGG_A

Cuts at:      0     760    1263 
   Size:        760     503
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1135    1167    1263 
   Size:       1135      32      96

  Fragments arranged by size: 

                1135      96      32
 
PspOMI G'GGCC_C

Cuts at:      0     911    1263 
   Size:        911     352
 
PspXI vC'TCGA_Gb

Cuts at:      0     866    1263 
   Size:        866     397
 
PvuII CAG'CTG

Cuts at:      0     805    1263 
   Size:        805     458
 
SacI G_AGCT'C

Cuts at:      0     927    1263 
   Size:        927     336
 
SmaI CCC'GGG

Cuts at:      0     223     352    1263 
   Size:        223     129     911

  Fragments arranged by size: 

                 911     223     129
 
SmlI C'TyrA_G

Cuts at:      0      95     866     916    1263 
   Size:         95     771      50     347

  Fragments arranged by size: 

                 771     347      95      50
 
StuI AGG'CCT

Cuts at:      0     116    1263 
   Size:        116    1147
 
StyI C'CwwG_G

Cuts at:      0     467     665    1263 
   Size:        467     198     598

  Fragments arranged by size: 

                 598     467     198
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     417    1263 
   Size:        417     846
 
TatI w'GTAC_w

Cuts at:      0     998    1263 
   Size:        998     265
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     631    1263 
   Size:        631     632
 
XhoI C'TCGA_G

Cuts at:      0      95     866    1263 
   Size:         95     771     397

  Fragments arranged by size: 

                 771     397      95
 
XmaI C'CCGG_G

Cuts at:      0     221     350    1263 
   Size:        221     129     913

  Fragments arranged by size: 

                 913     221     129
 

 Enzymes that do cut:

     AcuI   AflIII     AgeI     AleI     AlfI     AloI    AlwNI     ApaI
     ApoI     AvaI    BamHI     BanI    BanII    BfrBI     BlpI Bme1580I
     BmrI    BpuEI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BsrFI    BstBI    BstXI    BstYI   Bsu36I     BtgI     DrdI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI    Hin4I
   HincII  HindIII     MmeI     MslI   MspA1I     NcoI     NsiI     NspI
     PciI    PflMI     PfoI     PpiI   PspOMI    PspXI    PvuII     SacI
     SmaI     SmlI     StuI     StyI    TaqII     TatI     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AhdI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII    BmgBI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI   BstEII  BstZ17I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EaeI     EagI    EcoNI    EcoRV
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PmeI     PmlI    PpuMI    PshAI     PsiI
     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI  Tth111I
     XbaI     XmnI     ZraI
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