Restriction map for pF1KA1598
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BcnI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRV       EcoT22I     FauI        
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshBI       PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SfiI        SfoI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
SspI        StuI        SwaI        Tth111I     Van91I      
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII       1130  TCCGGA               T^CCGGA
AciI          392  CCGC                 CCGC(-3/-1)
AclI          285  AACGTT               AA^CGTT
AlwNI         922  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1627  GGGCCC               GGGCC^C
Asp700I       157  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         762  GAGCTC               G[AT]GC[AT]^C
BalI          314  TGGCCA               TGG^CCA
BanI          388  GGTGCC               G^G[CT][GA]CC
BciVI        1537  GTATCC               GTATCC(6/5)
BclI          774  TGATCA               T^GATCA
BglII        1209  AGATCT               A^GATCT
BsaJI        1658  CCAAGG               C^C[ACGT][ACGT]GG
BsaWI        1130  TCCGGA               [AT]^CCGG[AT]
BseAI        1130  TCCGGA               T^CCGGA
BsgI         1331  GTGCAG               GTGCAG(16/14)
BsiHKAI       762  GAGCTC               G[AT]GC[AT]^C
Bsp1407I      308  TGTACA               T^GTACA
BspEI        1130  TCCGGA               T^CCGGA
BsrGI         308  TGTACA               T^GTACA
BstAPI       1685  GCATTGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EaeI          314  TGGCCA               [CT]^GGCC[GA]
EcoNI        1612  CCTGAGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I      1658  CCAAGG               C^C[AT][AT]GG
FbaI          774  TGATCA               T^GATCA
HapII        1131  CCGG                 C^CGG
HgaI         1443  GCGTC                GACGC(5/10)
HpaII        1131  CCGG                 C^CGG
MluNI         314  TGGCCA               TGG^CCA
MroI         1130  TCCGGA               T^CCGGA
MscI          314  TGGCCA               TGG^CCA
MspA1I         25  CAGCTG               C[AC]G^C[GT]G
MspI         1131  CCGG                 C^CGG
NspI          302  GCATGT               [GA]CATG^[CT]
PpuMI         674  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         399  GACTTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      285  AACGTT               AA^CGTT
PvuII          25  CAGCTG               CAG^CTG
SacI          762  GAGCTC               GAGCT^C
SapI          871  GAAGAGC              GCTCTTC(1/4)
SexAI        1781  ACCAGGT              A^CC[AT]GGT
SmlI          502  CTCAAG               C^T[CT][GA]AG
SpeI         1378  ACTAGT               A^CTAGT
SspBI         308  TGTACA               T^GTACA
StyI         1658  CCAAGG               C^C[AT][AT]GG
XbaI          936  TCTAGA               T^CTAGA
XcmI         1517  CCATGCCAGTGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          157  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AvaII         325  GGACC                G^G[AT]CC
              675  GGACC               
BanII         762  GAGCTC               G[GA]GC[CT]^C
             1627  GGGCCC              
BmyI          762  GAGCTC               G[AGT]GC[ACT]^C
             1627  GGGCCC              
Bsp1286I      762  GAGCTC               G[AGT]GC[ACT]^C
             1627  GGGCCC              
BstBI         680  TTCGAA               TT^CGAA
             1314  TTCGAA              
BstXI         485  CCATTGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1143  CCACCCCAGTGG        
Cac8I          54  GCAAGC               GC[ACGT]^[ACGT]GC
             1221  GCAAGC              
DraI          564  TTTAAA               TTT^AAA
              999  TTTAAA              
HaeIII        315  GGCC                 GG^CC
             1628  GGCC                
NspV          680  TTCGAA               TT^CGAA
             1314  TTCGAA              
PleI          694  GAGTC                GAGTC(4/5)
             1507  GAGTC               
PstI           28  CTGCAG               CTGCA^G
              757  CTGCAG              
SfuI          680  TTCGAA               TT^CGAA
             1314  TTCGAA              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
BbsI        BbvI        BfaI        BpmI        BpuAI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoO109I    EcoRI       EcoRII      Fnu4HI      FokI        
HindIII     HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com