Restriction map for pF1KA1598
 (Linear) (Six-base) MAPSORT of: KIAA1598.seq  Check: 1968  from: 1  to: 1893



KIAA1598.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AclI AA'CG_TT

Cuts at:      0     286    1893 
   Size:        286    1607
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     269     362    1001    1217    1893 
   Size:         67     202      93     639     216     676

  Fragments arranged by size: 

                 676     639     216     202      93      67
 
AleI CACnn'nnGTG

Cuts at:      0    1148    1893 
   Size:       1148     745
 
AlwNI CAG_nnn'CTG

Cuts at:      0     927    1893 
   Size:        927     966
 
ApaI G_GGCC'C

Cuts at:      0    1631    1893 
   Size:       1631     262
 
ApoI r'AATT_y

Cuts at:      0     160     170     567     839     975     996    1047    1585 
   Size:        160      10     397     272     136      21      51     538

Cuts at:   1585    1829    1893 
   Size:        244      64

  Fragments arranged by size: 

                 538     397     272     244     160     136      64      51
                  21      10
 
BanI G'GyrC_C

Cuts at:      0     388    1893 
   Size:        388    1505
 
BanII G_rGCy'C

Cuts at:      0     766    1631    1893 
   Size:        766     865     262

  Fragments arranged by size: 

                 865     766     262
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      80     235    1303    1856    1893 
   Size:         80     155    1068     553      37

  Fragments arranged by size: 

                1068     553     155      80      37
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1548    1893 
   Size:       1548     345
 
BclI T'GATC_A

Cuts at:      0     774    1893 
   Size:        774    1119
 
BglII A'GATC_T

Cuts at:      0    1209    1893 
   Size:       1209     684
 
Bme1580I G_kGCm'C

Cuts at:      0    1631    1893 
   Size:       1631     262
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1142    1366    1496    1673    1893 
   Size:       1142     224     130     177     220

  Fragments arranged by size: 

                1142     224     220     177     130
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     682     688     949    1006    1597    1893 
   Size:        682       6     261      57     591     296

  Fragments arranged by size: 

                 682     591     296     261      57       6
 
Bpu10I CC'TnA_GC

Cuts at:      0    1613    1893 
   Size:       1613     280
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     487    1893 
   Size:        487    1406
 
BsaWI w'CCGG_w

Cuts at:      0    1130    1893 
   Size:       1130     763
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     658    1058    1061    1893 
   Size:        658     400       3     832

  Fragments arranged by size: 

                 832     658     400       3
 
BseYI C'CCAG_C

Cuts at:      0     321     818    1893 
   Size:        321     497    1075

  Fragments arranged by size: 

                1075     497     321
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1352    1893 
   Size:       1352     541
 
BsiHKAI G_wGCw'C

Cuts at:      0     766    1893 
   Size:        766    1127
 
Bsp1286I G_dGCh'C

Cuts at:      0     766    1631    1893 
   Size:        766     865     262

  Fragments arranged by size: 

                 865     766     262
 
BspEI T'CCGG_A

Cuts at:      0    1130    1893 
   Size:       1130     763
 
BsrGI T'GTAC_A

Cuts at:      0     308    1893 
   Size:        308    1585
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1691    1893 
   Size:       1691     202
 
BstBI TT'CG_AA

Cuts at:      0     681    1315    1893 
   Size:        681     634     578

  Fragments arranged by size: 

                 681     634     578
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     492    1150    1893 
   Size:        492     658     743

  Fragments arranged by size: 

                 743     658     492
 
BstYI r'GATC_y

Cuts at:      0    1209    1746    1797    1893 
   Size:       1209     537      51      96

  Fragments arranged by size: 

                1209     537      96      51
 
BtsI GCAGTG_nn'

Cuts at:      0    1161    1340    1893 
   Size:       1161     179     553

  Fragments arranged by size: 

                1161     553     179
 
DraI TTT'AAA

Cuts at:      0     566    1001    1893 
   Size:        566     435     892

  Fragments arranged by size: 

                 892     566     435
 
EaeI y'GGCC_r

Cuts at:      0     314    1893 
   Size:        314    1579
 
EarI CTCTTCn'nnn_

Cuts at:      0      11     338     629     827     866     977    1226    1893 
   Size:         11     327     291     198      39     111     249     667

  Fragments arranged by size: 

                 667     327     291     249     198     111      39      11
            
 
EcoICRI GAG'CTC

Cuts at:      0     764    1893 
   Size:        764    1129
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     269     362     682     688     949    1001    1006 
   Size:         67     202      93     320       6     261      52       5

Cuts at:   1006    1217    1597    1893 
   Size:        211     380     296

  Fragments arranged by size: 

                 380     320     296     261     211     202      93      67
                  52       6       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1616    1893 
   Size:       1616     277
 
EcoO109I rG'GnC_Cy

Cuts at:      0     675    1627    1628    1893 
   Size:        675     952       1     265

  Fragments arranged by size: 

                 952     675     265       1
 
EcoRI G'AATT_C

Cuts at:      0     839     975    1047    1585    1893 
   Size:        839     136      72     538     308

  Fragments arranged by size: 

                 839     538     308     136      72
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1428    1460    1498    1530    1893 
   Size:       1428      32      38      32     363

  Fragments arranged by size: 

                1428     363      38      32      32
 
HindIII A'AGCT_T

Cuts at:      0     251     791    1389    1893 
   Size:        251     540     598     504

  Fragments arranged by size: 

                 598     540     504     251
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1507    1825    1893 
   Size:       1507     318      68

  Fragments arranged by size: 

                1507     318      68
 
MscI TGG'CCA

Cuts at:      0     316    1893 
   Size:        316    1577
 
MslI CAynn'nnrTG

Cuts at:      0    1123    1148    1522    1893 
   Size:       1123      25     374     371

  Fragments arranged by size: 

                1123     374     371      25
 
MspA1I CmG'CkG

Cuts at:      0      27    1893 
   Size:         27    1866
 
NspI r_CATG'y

Cuts at:      0     306    1893 
   Size:        306    1587
 
PpuMI rG'GwC_Cy

Cuts at:      0     675    1893 
   Size:        675    1218
 
PshAI GACnn'nnGTC

Cuts at:      0     403    1893 
   Size:        403    1490
 
PspOMI G'GGCC_C

Cuts at:      0    1627    1893 
   Size:       1627     266
 
PstI C_TGCA'G

Cuts at:      0      32     761    1893 
   Size:         32     729    1132

  Fragments arranged by size: 

                1132     729      32
 
PvuII CAG'CTG

Cuts at:      0      27    1893 
   Size:         27    1866
 
SacI G_AGCT'C

Cuts at:      0     766    1893 
   Size:        766    1127
 
SapI GCTCTTCn'nnn_

Cuts at:      0     866    1893 
   Size:        866    1027
 
SexAI A'CCwGG_T

Cuts at:      0    1781    1893 
   Size:       1781     112
 
SfcI C'TryA_G

Cuts at:      0      28     374     629     757    1893 
   Size:         28     346     255     128    1136

  Fragments arranged by size: 

                1136     346     255     128      28
 
SmlI C'TyrA_G

Cuts at:      0     502    1893 
   Size:        502    1391
 
SpeI A'CTAG_T

Cuts at:      0    1378    1893 
   Size:       1378     515
 
StyI C'CwwG_G

Cuts at:      0    1658    1893 
   Size:       1658     235
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     832    1308    1893 
   Size:        832     476     585

  Fragments arranged by size: 

                 832     585     476
 
TatI w'GTAC_w

Cuts at:      0     308     411    1893 
   Size:        308     103    1482

  Fragments arranged by size: 

                1482     308     103
 
XbaI T'CTAG_A

Cuts at:      0     936    1893 
   Size:        936     957
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1524    1893 
   Size:       1524     369
 
XmnI GAAnn'nnTTC

Cuts at:      0     161    1893 
   Size:        161    1732
 

 Enzymes that do cut:

     AclI     AcuI     AleI    AlwNI     ApaI     ApoI     BanI    BanII
     BbsI    BciVI     BclI    BglII Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI
    BsrGI   BstAPI    BstBI    BstXI    BstYI     BtsI     DraI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    Hin4I  HindIII
     MmeI     MscI     MslI   MspA1I     NspI    PpuMI    PshAI   PspOMI
     PstI    PvuII     SacI     SapI    SexAI     SfcI     SmlI     SpeI
     StyI    TaqII     TatI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BfrBI     BglI
     BlpI    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI     EciI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XhoI     XmaI     ZraI
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