Restriction map for pF1KA1597
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
AocI        Aor51HI     ApaI        AscI        AspEI       
AspI        AviII       BbeI        BbrPI       BbvCI       
BcgI        BclI        BcnI        BfrI        BglI        
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoT22I     FbaI        FseI        
FspI        HaeII       HapII       HgaI        Hin1I       
HpaI        HpaII       KasI        KspI        MaeII       
MamI        MluI        MroI        MspI        MvnI        
NaeI        NarI        NciI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SwaI        TaqI        Tth111I     TthHB8I     
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I       2138  GGTACC               G^GTACC
Alw44I       2263  GTGCAC               G^TGCAC
ApaLI        2263  GTGCAC               G^TGCAC
AseI         1204  ATTAAT               AT^TAAT
AsnI         1204  ATTAAT               AT^TAAT
Asp718I      2138  GGTACC               G^GTACC
AspHI        2263  GTGCAC               G[AT]GC[AT]^C
AvaI          339  CCCGAG               C^[CT]CG[GA]G
AvrII        2070  CCTAGG               C^CTAGG
BalI          105  TGGCCA               TGG^CCA
BamHI         767  GGATCC               G^GATCC
BanII         755  GGGCTC               G[GA]GC[CT]^C
BciVI        2046  GGATAC               GTATCC(6/5)
BglII        2448  AGATCT               A^GATCT
BlnI         2070  CCTAGG               C^CTAGG
BlpI          208  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      208  GCTGAGC              GC^T[ACGT]AGC
BsaI         1400  GAGACC               GGTCTC(1/5)
BsiHKAI      2263  GTGCAC               G[AT]GC[AT]^C
BsoBI         339  CCCGAG               C^[CT]CG[GA]G
CelII         208  GCTGAGC              GC^T[ACGT]AGC
DraI          693  TTTAAA               TTT^AAA
EaeI          105  TGGCCA               [CT]^GGCC[GA]
EcoNI        2070  CCTAGGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1260  GAATTC               G^AATTC
EcoRV         163  GATATC               GAT^ATC
FauI           15  GCGGG                CCCGC(4/6)
HindIII      2242  AAGCTT               A^AGCTT
KpnI         2138  GGTACC               GGTAC^C
MluNI         105  TGGCCA               TGG^CCA
MscI          105  TGGCCA               TGG^CCA
PpuMI         634  GGGTCCC              [GA]G^G[AT]CC[CT]
PshAI        1244  GACCACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1204  ATTAAT               AT^TAAT
PstI          959  CTGCAG               CTGCA^G
SapI          307  GAAGAGC              GCTCTTC(1/4)
SspI         1774  AATATT               AAT^ATT
XcmI         2501  CCAACCAATTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AlwI          768  GATCC                GGATC(4/5)
              767  GGATC               
AlwNI          85  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2228  CAGTCCCTG           
AvaII         635  GGTCC                G^G[AT]CC
             2484  GGACC               
BanI         1380  GGTGCC               G^G[CT][GA]CC
             2138  GGTACC              
BmyI          755  GGGCTC               G[AGT]GC[ACT]^C
             2263  GTGCAC              
Bsp1286I      755  GGGCTC               G[AGT]GC[ACT]^C
             2263  GTGCAC              
BstAPI        609  GCAGACTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2161  GCACCAGTTGC         
BstYI         767  GGATCC               [GA]^GATC[CT]
             2448  AGATCT              
DraII         193  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              634  GGGTCCC             
DsaI          153  CCATGG               C^C[GA][CT]GG
              999  CCATGG              
EcoO109I      193  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              634  GGGTCCC             
HincII        262  GTCAAC               GT[CT]^[GA]AC
              564  GTCAAC              
HindII        262  GTCAAC               GT[CT]^[GA]AC
              564  GTCAAC              
MfeI         1787  CAATTG               C^AATTG
             2128  CAATTG              
MflI          767  GGATCC               [GA]^GATC[CT]
             2448  AGATCT              
MslI         2268  CATCTGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2418  CACTATGGTG          
MunI         1787  CAATTG               C^AATTG
             2128  CAATTG              
NcoI          153  CCATGG               C^CATGG
              999  CCATGG              
NdeI         1151  CATATG               CA^TATG
             1883  CATATG              
NspI          945  GCATGT               [GA]CATG^[CT]
             1818  GCATGT              
PvuII          88  CAGCTG               CAG^CTG
              206  CAGCTG              
SfcI          959  CTGCAG               C^T[GA][CT]AG
             2384  CTGTAG              
SmlI          772  CTCAAG               C^T[CT][GA]AG
             1143  CTCAAG              
XbaI         1396  TCTAGA               T^CTAGA
             1488  TCTAGA              
XhoII         767  GGATCC               [GA]^GATC[CT]
             2448  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
Asp700I     BbsI        BbvI        BfaI        BpmI        
BpuAI       BsaJI       BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HhaI        HinP1I      HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MspA1I      
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PflMI       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StuI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Van91I      XmnI        
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