Restriction map for pF1KA1597
 (Linear) (Six-base) MAPSORT of: KIAA1597.seq  Check: 2982  from: 1  to: 2685



KIAA1597.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

Acc65I G'GTAC_C

Cuts at:      0    2138    2685 
   Size:       2138     547
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     112    1583    1828    2167    2465    2567    2579 
   Size:         49      63    1471     245     339     298     102      12

Cuts at:   2579    2685 
   Size:        106

  Fragments arranged by size: 

                1471     339     298     245     106     102      63      49
                  12
 
AleI CACnn'nnGTG

Cuts at:      0    2422    2685 
   Size:       2422     263
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      35    2685 
   Size:         35    2650
 
AlwNI CAG_nnn'CTG

Cuts at:      0      90    2233    2685 
   Size:         90    2143     452

  Fragments arranged by size: 

                2143     452      90
 
ApaLI G'TGCA_C

Cuts at:      0    2263    2685 
   Size:       2263     422
 
ApoI r'AATT_y

Cuts at:      0     881    1018    1260    1616    2319    2685 
   Size:        881     137     242     356     703     366

  Fragments arranged by size: 

                 881     703     366     356     242     137
 
AseI AT'TA_AT

Cuts at:      0    1205    2685 
   Size:       1205    1480
 
AvaI C'yCGr_G

Cuts at:      0     339    2685 
   Size:        339    2346
 
AvrII C'CTAG_G

Cuts at:      0    2070    2685 
   Size:       2070     615
 
BamHI G'GATC_C

Cuts at:      0     767    2685 
   Size:        767    1918
 
BanI G'GyrC_C

Cuts at:      0    1380    2138    2685 
   Size:       1380     758     547

  Fragments arranged by size: 

                1380     758     547
 
BanII G_rGCy'C

Cuts at:      0     759    2685 
   Size:        759    1926
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     914    1499    2161    2380    2512    2685 
   Size:        914     585     662     219     132     173

  Fragments arranged by size: 

                 914     662     585     219     173     132
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2040    2685 
   Size:       2040     645
 
BglII A'GATC_T

Cuts at:      0    2448    2685 
   Size:       2448     237
 
BlpI GC'TnA_GC

Cuts at:      0     209    2685 
   Size:        209    2476
 
Bme1580I G_kGCm'C

Cuts at:      0    2267    2685 
   Size:       2267     418
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1424    1827    2115    2685 
   Size:       1424     403     288     570

  Fragments arranged by size: 

                1424     570     403     288
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     773     787    2685 
   Size:        773      14    1898

  Fragments arranged by size: 

                1898     773      14
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     401     768     952    2194    2276    2685 
   Size:        269     132     367     184    1242      82     409

  Fragments arranged by size: 

                1242     409     367     269     184     132      82
 
Bpu10I CC'TnA_GC

Cuts at:      0    1097    1367    2302    2685 
   Size:       1097     270     935     383

  Fragments arranged by size: 

                1097     935     383     270
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     757    1128    2685 
   Size:        757     371    1557

  Fragments arranged by size: 

                1557     757     371
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1394    2685 
   Size:       1394    1291
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2192    2222    2685 
   Size:       2192      30     463

  Fragments arranged by size: 

                2192     463      30
 
BseYI C'CCAG_C

Cuts at:      0     248    2685 
   Size:        248    2437
 
BsiHKAI G_wGCw'C

Cuts at:      0    2267    2685 
   Size:       2267     418
 
BsmI GAATG_Cn'

Cuts at:      0     897    1215    1609    2287    2663    2685 
   Size:        897     318     394     678     376      22

  Fragments arranged by size: 

                 897     678     394     376     318      22
 
Bsp1286I G_dGCh'C

Cuts at:      0     759    2267    2685 
   Size:        759    1508     418

  Fragments arranged by size: 

                1508     759     418
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     615    2167    2685 
   Size:        615    1552     518

  Fragments arranged by size: 

                1552     615     518
 
BstYI r'GATC_y

Cuts at:      0     767    2448    2685 
   Size:        767    1681     237

  Fragments arranged by size: 

                1681     767     237
 
BtgI C'CryG_G

Cuts at:      0     153     999    2685 
   Size:        153     846    1686

  Fragments arranged by size: 

                1686     846     153
 
BtsI GCAGTG_nn'

Cuts at:      0     968    1728    2296    2644    2685 
   Size:        968     760     568     348      41

  Fragments arranged by size: 

                 968     760     568     348      41
 
DraI TTT'AAA

Cuts at:      0     695    2685 
   Size:        695    1990
 
EaeI y'GGCC_r

Cuts at:      0     105    2685 
   Size:        105    2580
 
EarI CTCTTCn'nnn_

Cuts at:      0      25      38     184     302     311     418     921    1073 
   Size:         25      13     146     118       9     107     503     152

Cuts at:   1073    2685 
   Size:       1612

  Fragments arranged by size: 

                1612     503     152     146     118     107      25      13
                   9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     112     269     401     768     952    1583    1828 
   Size:         49      63     157     132     367     184     631     245

Cuts at:   1828    2167    2194    2276    2465    2567    2579    2685 
   Size:        339      27      82     189     102      12     106

  Fragments arranged by size: 

                 631     367     339     245     189     184     157     132
                 106     102      82      63      49      27      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2074    2685 
   Size:       2074     611
 
EcoO109I rG'GnC_Cy

Cuts at:      0     194     635    2685 
   Size:        194     441    2050

  Fragments arranged by size: 

                2050     441     194
 
EcoRI G'AATT_C

Cuts at:      0    1260    2685 
   Size:       1260    1425
 
EcoRV GAT'ATC

Cuts at:      0     165    2685 
   Size:        165    2520
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1971    2685 
   Size:       1971     714
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     417     449     529     561     697     729    1279    1311 
   Size:        417      32      80      32     136      32     550      32

Cuts at:   1311    1333    1365    1486    1518    2685 
   Size:         22      32     121      32    1167

  Fragments arranged by size: 

                1167     550     417     136     121      80      32      32
                  32      32      32      32      22
 
HincII GTy'rAC

Cuts at:      0     264     566    2685 
   Size:        264     302    2119

  Fragments arranged by size: 

                2119     302     264
 
HindIII A'AGCT_T

Cuts at:      0    2242    2685 
   Size:       2242     443
 
KpnI G_GTAC'C

Cuts at:      0    2142    2685 
   Size:       2142     543
 
MfeI C'AATT_G

Cuts at:      0    1787    2128    2685 
   Size:       1787     341     557

  Fragments arranged by size: 

                1787     557     341
 
MscI TGG'CCA

Cuts at:      0     107    2685 
   Size:        107    2578
 
MslI CAynn'nnrTG

Cuts at:      0    2272    2422    2685 
   Size:       2272     150     263

  Fragments arranged by size: 

                2272     263     150
 
MspA1I CmG'CkG

Cuts at:      0      15      90     208    1855    2685 
   Size:         15      75     118    1647     830

  Fragments arranged by size: 

                1647     830     118      75      15
 
NcoI C'CATG_G

Cuts at:      0     153     999    2685 
   Size:        153     846    1686

  Fragments arranged by size: 

                1686     846     153
 
NdeI CA'TA_TG

Cuts at:      0    1152    1884    2685 
   Size:       1152     732     801

  Fragments arranged by size: 

                1152     801     732
 
NspI r_CATG'y

Cuts at:      0     949    1822    2685 
   Size:        949     873     863

  Fragments arranged by size: 

                 949     873     863
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1207    2233    2421    2511    2630    2685 
   Size:       1207    1026     188      90     119      55

  Fragments arranged by size: 

                1207    1026     188     119      90      55
 
PpuMI rG'GwC_Cy

Cuts at:      0     635    2685 
   Size:        635    2050
 
PshAI GACnn'nnGTC

Cuts at:      0    1248    2685 
   Size:       1248    1437
 
PsiI TTA'TAA

Cuts at:      0     821    2685 
   Size:        821    1864
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2419    2451    2685 
   Size:       2419      32     234

  Fragments arranged by size: 

                2419     234      32
 
PstI C_TGCA'G

Cuts at:      0     963    2685 
   Size:        963    1722
 
PvuII CAG'CTG

Cuts at:      0      90     208    2685 
   Size:         90     118    2477

  Fragments arranged by size: 

                2477     118      90
 
SanDI GG'GwC_CC

Cuts at:      0     635    2685 
   Size:        635    2050
 
SapI GCTCTTCn'nnn_

Cuts at:      0     302    2685 
   Size:        302    2383
 
SfcI C'TryA_G

Cuts at:      0     959    2384    2685 
   Size:        959    1425     301

  Fragments arranged by size: 

                1425     959     301
 
SmlI C'TyrA_G

Cuts at:      0     772    1143    2685 
   Size:        772     371    1542

  Fragments arranged by size: 

                1542     772     371
 
SspI AAT'ATT

Cuts at:      0    1776    2685 
   Size:       1776     909
 
StuI AGG'CCT

Cuts at:      0     623    1894    2442    2685 
   Size:        623    1271     548     243

  Fragments arranged by size: 

                1271     623     548     243
 
StyI C'CwwG_G

Cuts at:      0     125     153     999    2070    2685 
   Size:        125      28     846    1071     615

  Fragments arranged by size: 

                1071     846     615     125      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     240    2262    2685 
   Size:        240    2022     423

  Fragments arranged by size: 

                2022     423     240
 
XbaI T'CTAG_A

Cuts at:      0    1396    1488    2685 
   Size:       1396      92    1197

  Fragments arranged by size: 

                1396    1197      92
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2508    2685 
   Size:       2508     177
 
XmnI GAAnn'nnTTC

Cuts at:      0     812    1082    1119    2685 
   Size:        812     270      37    1566

  Fragments arranged by size: 

                1566     812     270      37
 

 Enzymes that do cut:

   Acc65I     AcuI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
     AvaI    AvrII    BamHI     BanI    BanII     BbsI    BciVI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaXI    BseYI  BsiHKAI     BsmI Bsp1286I   BstAPI    BstYI     BtgI
     BtsI     DraI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI    Hin4I   HincII  HindIII     KpnI     MfeI     MscI
     MslI   MspA1I     NcoI     NdeI     NspI    PflMI    PpuMI    PshAI
     PsiI     PsrI     PstI    PvuII    SanDI     SapI     SfcI     SmlI
     SspI     StuI     StyI    TaqII     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI     AscI    AsiSI     BaeI     BbeI    BbvCI
     BcgI     BclI    BfrBI     BglI    BmgBI     BmtI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
   DraIII     DrdI     EagI     EciI  EcoICRI     FseI     FspI    FspAI
    HaeII     HpaI     KasI     MluI     MmeI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI   PspOMI    PspXI     PvuI    RsrII     SacI
    SacII     SalI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI     TatI
     TstI  Tth111I     XhoI     XmaI     ZraI
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