Restriction map for pF1KA1590
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        AhdI        Aor51HI     AscI        Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BciVI       BfrI        
BglI        BlnI        BsaAI       BseAI       BsiEI       
BsiWI       BsoBI       Bsp1407I    BspEI       BspLU11I    
BsrFI       BsrGI       BssHII      Bst1107I    BstEII      
BstPI       BstXI       BstZ17I     Cfr10I      CpoI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRV       EcoT22I     FseI        
FspI        HpaI        KasI        KpnI        KspI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NcoI        NgoMIV      NotI        NruI        
NsiI        NspI        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SbfI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AatII         652  GACGTC               GACGT^C
AccII        2110  CGCG                 CG^CG
AflIII        468  ACGTGT               A^C[GA][CT]GT
Alw44I       3887  GTGCAC               G^TGCAC
ApaI         3020  GGGCCC               GGGCC^C
ApaLI        3887  GTGCAC               G^TGCAC
AseI         1090  ATTAAT               AT^TAAT
AsnI         1090  ATTAAT               AT^TAAT
BanI         3469  GGCACC               G^G[CT][GA]CC
BbvCI        2049  GCTGAGG              CCTCAGC(-5/-2)
BcgI          248  GCACAGATGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         3902  TGATCA               T^GATCA
BlpI         3163  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3163  GCTGAGC              GC^T[ACGT]AGC
BsmBI        1445  GAGACG               CGTCTC(1/5)
BspDI        1375  ATCGAT               AT^CGAT
BspHI         989  TCATGA               T^CATGA
BstAPI       2271  GCAGGTCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        2110  CGCG                 CG^CG
CelII        3163  GCTGAGC              GC^T[ACGT]AGC
ClaI         1375  ATCGAT               AT^CGAT
DrdI          383  GACTCTTCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1457  CCACGG               C^C[GA][CT]GG
EcoNI        3177  CCTGGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         3902  TGATCA               T^GATCA
HaeII        3009  GGCGCT               [GA]GCGC^[CT]
MvnI         2110  CGCG                 CG^CG
NdeI          299  CATATG               CA^TATG
NheI         3151  GCTAGC               G^CTAGC
PshBI        1090  ATTAAT               AT^TAAT
RcaI          989  TCATGA               T^CATGA
SpeI         3975  ACTAGT               A^CTAGT
XbaI         2614  TCTAGA               T^CTAGA
XcmI         2225  CCAAGCTTGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AcyI          652  GACGTC               G[GA]^CG[CT]C
             3383  GACGCC              
AocI         1317  CCTCAGG              CC^T[ACGT]AGG
             4105  CCTGAGG             
Asp700I      1216  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2383  GAAGGCATTC          
BalI         3026  TGGCCA               TGG^CCA
             3777  TGGCCA              
BanII        3020  GGGCCC               G[GA]GC[CT]^C
             3142  GGGCTC              
BbsI         3835  GAAGAC               GAAGAC(2/6)
             3979  GTCTTC              
BglII         477  AGATCT               A^GATCT
             3576  AGATCT              
BpuAI        3835  GAAGAC               GAAGAC(2/6)
             3979  GTCTTC              
BsaBI        1368  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3394  GATTTGCATC          
BsaHI         652  GACGTC               G[GA]^CG[CT]C
             3383  GACGCC              
BsaWI         623  ACCGGA               [AT]^CCGG[AT]
              784  ACCGGA              
BsgI          773  GTGCAG               GTGCAG(16/14)
             1939  GTGCAG              
BsmI         2387  GCATTC               GAATGC(1/-1)
             3857  GCATTC              
BsrBI        1891  GAGCGG               CCGCTC(-3/-3)
             3016  GAGCGG              
BssSI        2281  CTCGTG               CACGAG(-5/-1)
             2420  CTCGTG              
BstBI         431  TTCGAA               TT^CGAA
             2476  TTCGAA              
Bsu36I       1317  CCTCAGG              CC^T[ACGT]AGG
             4105  CCTGAGG             
DraI         1416  TTTAAA               TTT^AAA
             2589  TTTAAA              
Eco81I       1317  CCTCAGG              CC^T[ACGT]AGG
             4105  CCTGAGG             
HgaI         3383  GACGC                GACGC(5/10)
             2111  GCGTC               
Hin1I         652  GACGTC               G[GA]^CG[CT]C
             3383  GACGCC              
HindIII       422  AAGCTT               A^AGCTT
             2227  AAGCTT              
MamI         1368  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3394  GATTTGCATC          
MluNI        3026  TGGCCA               TGG^CCA
             3777  TGGCCA              
MscI         3026  TGGCCA               TGG^CCA
             3777  TGGCCA              
NspV          431  TTCGAA               TT^CGAA
             2476  TTCGAA              
PflMI         407  CCACCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1256  CCAAGGAATGG         
PvuII        2893  CAGCTG               CAG^CTG
             3217  CAGCTG              
SapI         2296  GAAGAGC              GCTCTTC(1/4)
             3088  GAAGAGC             
ScaI         1039  AGTACT               AGT^ACT
             1393  AGTACT              
SexAI        2525  ACCTGGT              A^CC[AT]GGT
             3326  ACCTGGT             
SfuI          431  TTCGAA               TT^CGAA
             2476  TTCGAA              
SspI          820  AATATT               AAT^ATT
             1291  AATATT              
Van91I        407  CCACCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1256  CCAAGGAATGG         
XmnI         1216  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2383  GAAGGCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRI       
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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