Restriction map for pF1KA1590
 (Linear) (Six-base) MAPSORT of: KIAA1590.seq  Check: 4332  from: 1  to: 4176



KIAA1590.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1017    4176 
   Size:       1017    3159
 
AatII G_ACGT'C

Cuts at:      0     656    4176 
   Size:        656    3520
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     372     458    1207    1698    3373    3986    4021    4176 
   Size:        372      86     749     491    1675     613      35     155

  Fragments arranged by size: 

                1675     749     613     491     372     155      86      35
            
 
AflIII A'CryG_T

Cuts at:      0     468    4176 
   Size:        468    3708
 
AleI CACnn'nnGTG

Cuts at:      0    2659    3754    4176 
   Size:       2659    1095     422

  Fragments arranged by size: 

                2659    1095     422
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3122    4176 
   Size:       3122    1054
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      52      84    2561    2593    4176 
   Size:         52      32    2477      32    1583

  Fragments arranged by size: 

                2477    1583      52      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     640    2334    3219    3535    3741    4176 
   Size:        640    1694     885     316     206     435

  Fragments arranged by size: 

                1694     885     640     435     316     206
 
ApaI G_GGCC'C

Cuts at:      0    3024    4176 
   Size:       3024    1152
 
ApaLI G'TGCA_C

Cuts at:      0    3887    4176 
   Size:       3887     289
 
ApoI r'AATT_y

Cuts at:      0     342     455     703    1789    2032    2479    2786    2920 
   Size:        342     113     248    1086     243     447     307     134

Cuts at:   2920    3655    3925    4176 
   Size:        735     270     251

  Fragments arranged by size: 

                1086     735     447     342     307     270     251     248
                 243     134     113
 
AseI AT'TA_AT

Cuts at:      0    1091    4176 
   Size:       1091    3085
 
BanI G'GyrC_C

Cuts at:      0    3469    4176 
   Size:       3469     707
 
BanII G_rGCy'C

Cuts at:      0    3024    3146    4176 
   Size:       3024     122    1030

  Fragments arranged by size: 

                3024    1030     122
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    3842    3972    4176 
   Size:       3842     130     204

  Fragments arranged by size: 

                3842     204     130
 
BbvCI CC'TCA_GC

Cuts at:      0    2050    4176 
   Size:       2050    2126
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     237     271    4176 
   Size:        237      34    3905

  Fragments arranged by size: 

                3905     237      34
 
BclI T'GATC_A

Cuts at:      0    3902    4176 
   Size:       3902     274
 
BglII A'GATC_T

Cuts at:      0     477    3576    4176 
   Size:        477    3099     600

  Fragments arranged by size: 

                3099     600     477
 
BlpI GC'TnA_GC

Cuts at:      0    3164    4176 
   Size:       3164    1012
 
Bme1580I G_kGCm'C

Cuts at:      0    3024    3079    3891    4176 
   Size:       3024      55     812     285

  Fragments arranged by size: 

                3024     812     285      55
 
BmgBI CAC'GTC

Cuts at:      0    2941    4176 
   Size:       2941    1235
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     143     650    1554    4176 
   Size:        143     507     904    2622

  Fragments arranged by size: 

                2622     904     507     143
 
BmtI G_CTAG'C

Cuts at:      0    3155    4176 
   Size:       3155    1021
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2821    4176 
   Size:       2821    1355
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     368    1909    1930    3034    3055    3130    3178    3199 
   Size:        368    1541      21    1104      21      75      48      21

Cuts at:   3199    3553    4176 
   Size:        354     623

  Fragments arranged by size: 

                1541    1104     623     368     354      75      48      21
                  21      21
 
Bpu10I CC'TnA_GC

Cuts at:      0     864    1678    2050    4028    4176 
   Size:        864     814     372    1978     148

  Fragments arranged by size: 

                1978     864     814     372     148
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1148    2651    4118    4176 
   Size:       1148    1503    1467      58

  Fragments arranged by size: 

                1503    1467    1148      58
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     671    1906    2092    4176 
   Size:        671    1235     186    2084

  Fragments arranged by size: 

                2084    1235     671     186
 
BsaBI GATnn'nnATC

Cuts at:      0    1372    3398    4176 
   Size:       1372    2026     778

  Fragments arranged by size: 

                2026    1372     778
 
BsaHI Gr'CG_yC

Cuts at:      0     653    3384    4176 
   Size:        653    2731     792

  Fragments arranged by size: 

                2731     792     653
 
BsaWI w'CCGG_w

Cuts at:      0     623     784    4176 
   Size:        623     161    3392

  Fragments arranged by size: 

                3392     623     161
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      52      82    2338    2368    2717    2747    3629    3659 
   Size:         52      30    2256      30     349      30     882      30

Cuts at:   3659    4176 
   Size:        517

  Fragments arranged by size: 

                2256     882     517     349      52      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1978    2056    2269    2380    2461    3054    3869    4176 
   Size:       1978      78     213     111      81     593     815     307

  Fragments arranged by size: 

                1978     815     593     307     213     111      81      78
            
 
BseYI C'CCAG_C

Cuts at:      0    1916    4176 
   Size:       1916    2260
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     794    1960    4176 
   Size:        794    1166    2216

  Fragments arranged by size: 

                2216    1166     794
 
BsiHKAI G_wGCw'C

Cuts at:      0    1567    3891    4034    4176 
   Size:       1567    2324     143     142

  Fragments arranged by size: 

                2324    1567     143     142
 
BsmI GAATG_Cn'

Cuts at:      0    2387    3857    4176 
   Size:       2387    1470     319

  Fragments arranged by size: 

                2387    1470     319
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1439    4176 
   Size:       1439    2737
 
Bsp1286I G_dGCh'C

Cuts at:      0    1567    3024    3079    3146    3891    4034    4176 
   Size:       1567    1457      55      67     745     143     142

  Fragments arranged by size: 

                1567    1457     745     143     142      67      55
 
BspHI T'CATG_A

Cuts at:      0     989    4176 
   Size:        989    3187
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     653    1017    2262    4176 
   Size:        653     364    1245    1914

  Fragments arranged by size: 

                1914    1245     653     364
 
BsrBI CCG'CTC

Cuts at:      0    1893    3018    4176 
   Size:       1893    1125    1158

  Fragments arranged by size: 

                1893    1158    1125
 
BsrDI GCAATG_nn'

Cuts at:      0    1503    2933    3818    4176 
   Size:       1503    1430     885     358

  Fragments arranged by size: 

                1503    1430     885     358
 
BssSI C'ACGA_G

Cuts at:      0    2281    2420    4176 
   Size:       2281     139    1756

  Fragments arranged by size: 

                2281    1756     139
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2277    4176 
   Size:       2277    1899
 
BstBI TT'CG_AA

Cuts at:      0     432    2477    4176 
   Size:        432    2045    1699

  Fragments arranged by size: 

                2045    1699     432
 
BstYI r'GATC_y

Cuts at:      0     313     371     477     740    2576    3576    4176 
   Size:        313      58     106     263    1836    1000     600

  Fragments arranged by size: 

                1836    1000     600     313     263     106      58
 
Bsu36I CC'TnA_GG

Cuts at:      0    1318    4106    4176 
   Size:       1318    2788      70

  Fragments arranged by size: 

                2788    1318      70
 
BtgI C'CryG_G

Cuts at:      0    1457    4176 
   Size:       1457    2719
 
BtsI GCAGTG_nn'

Cuts at:      0    2924    4176 
   Size:       2924    1252
 
ClaI AT'CG_AT

Cuts at:      0    1376    4176 
   Size:       1376    2800
 
DraI TTT'AAA

Cuts at:      0    1418    2591    4176 
   Size:       1418    1173    1585

  Fragments arranged by size: 

                1585    1418    1173
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     389    4176 
   Size:        389    3787
 
EaeI y'GGCC_r

Cuts at:      0      17    3026    3777    4176 
   Size:         17    3009     751     399

  Fragments arranged by size: 

                3009     751     399      17
 
EarI CTCTTCn'nnn_

Cuts at:      0     141     391    1049    1681    1687    2033    2077    2090 
   Size:        141     250     658     632       6     346      44      13

Cuts at:   2090    2291    2357    2365    3083    4176 
   Size:        201      66       8     718    1093

  Fragments arranged by size: 

                1093     718     658     632     346     250     201     141
                  66      44      13       8       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     504    4176 
   Size:        504    3672
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     368     372     458    1207    1698    1909    1930    3034 
   Size:        368       4      86     749     491     211      21    1104

Cuts at:   3034    3055    3130    3178    3199    3373    3553    3986    4021 
   Size:         21      75      48      21     174     180     433      35

Cuts at:   4021    4176 
   Size:        155

  Fragments arranged by size: 

                1104     749     491     433     368     211     180     174
                 155      86      75      48      35      21      21      21
                   4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3181    4176 
   Size:       3181     995
 
EcoO109I rG'GnC_Cy

Cuts at:      0     541    1556    2376    2523    2800    3021    3269    4176 
   Size:        541    1015     820     147     277     221     248     907

  Fragments arranged by size: 

                1015     907     820     541     277     248     221     147
            
 
EcoRI G'AATT_C

Cuts at:      0    2479    2920    3925    4176 
   Size:       2479     441    1005     251

  Fragments arranged by size: 

                2479    1005     441     251
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     762     982    2881    4176 
   Size:        762     220    1899    1295

  Fragments arranged by size: 

                1899    1295     762     220
 
HaeII r_GCGC'y

Cuts at:      0    3013    4176 
   Size:       3013    1163
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     544     576    1521    1553    1741    1773    2596    2628 
   Size:        544      32     945      32     188      32     823      32

Cuts at:   2628    4176 
   Size:       1548

  Fragments arranged by size: 

                1548     945     823     544     188      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     944    3675    3965    4176 
   Size:        944    2731     290     211

  Fragments arranged by size: 

                2731     944     290     211
 
HindIII A'AGCT_T

Cuts at:      0     422    2227    4176 
   Size:        422    1805    1949

  Fragments arranged by size: 

                1949    1805     422
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     373    2151    2603    2741    4176 
   Size:        373    1778     452     138    1435

  Fragments arranged by size: 

                1778    1435     452     373     138
 
MscI TGG'CCA

Cuts at:      0    3028    3779    4176 
   Size:       3028     751     397

  Fragments arranged by size: 

                3028     751     397
 
MslI CAynn'nnrTG

Cuts at:      0     334    2281    2659    3754    4176 
   Size:        334    1947     378    1095     422

  Fragments arranged by size: 

                1947    1095     422     378     334
 
MspA1I CmG'CkG

Cuts at:      0    2895    3219    3544    4176 
   Size:       2895     324     325     632

  Fragments arranged by size: 

                2895     632     325     324
 
NdeI CA'TA_TG

Cuts at:      0     300    4176 
   Size:        300    3876
 
NheI G'CTAG_C

Cuts at:      0    3151    4176 
   Size:       3151    1025
 
PasI CC'CwG_GG

Cuts at:      0    3072    4176 
   Size:       3072    1104
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     413    1262    4176 
   Size:        413     849    2914

  Fragments arranged by size: 

                2914     849     413
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      52      84    4176 
   Size:         52      32    4092

  Fragments arranged by size: 

                4092      52      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1556    2376    2523    3269    4176 
   Size:       1556     820     147     746     907

  Fragments arranged by size: 

                1556     907     820     746     147
 
PsiI TTA'TAA

Cuts at:      0     281     461    4176 
   Size:        281     180    3715

  Fragments arranged by size: 

                3715     281     180
 
PspOMI G'GGCC_C

Cuts at:      0    3020    4176 
   Size:       3020    1156
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2708    2740    4176 
   Size:       2708      32    1436

  Fragments arranged by size: 

                2708    1436      32
 
PstI C_TGCA'G

Cuts at:      0    1764    2078    2741    3059    3424    3435    4176 
   Size:       1764     314     663     318     365      11     741

  Fragments arranged by size: 

                1764     741     663     365     318     314      11
 
PvuII CAG'CTG

Cuts at:      0    2895    3219    4176 
   Size:       2895     324     957

  Fragments arranged by size: 

                2895     957     324
 
SanDI GG'GwC_CC

Cuts at:      0    1556    3269    4176 
   Size:       1556    1713     907

  Fragments arranged by size: 

                1713    1556     907
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2291    3083    4176 
   Size:       2291     792    1093

  Fragments arranged by size: 

                2291    1093     792
 
ScaI AGT'ACT

Cuts at:      0    1041    1395    4176 
   Size:       1041     354    2781

  Fragments arranged by size: 

                2781    1041     354
 
SexAI A'CCwGG_T

Cuts at:      0    2525    3326    4176 
   Size:       2525     801     850

  Fragments arranged by size: 

                2525     850     801
 
SfcI C'TryA_G

Cuts at:      0    1760    2074    2725    2737    3055    3420    3431    4176 
   Size:       1760     314     651      12     318     365      11     745

  Fragments arranged by size: 

                1760     745     651     365     318     314      12      11
            
 
SmlI C'TyrA_G

Cuts at:      0    1163    2666    4133    4176 
   Size:       1163    1503    1467      43

  Fragments arranged by size: 

                1503    1467    1163      43
 
SpeI A'CTAG_T

Cuts at:      0    3975    4176 
   Size:       3975     201
 
SspI AAT'ATT

Cuts at:      0     822    1293    4176 
   Size:        822     471    2883

  Fragments arranged by size: 

                2883     822     471
 
StyI C'CwwG_G

Cuts at:      0     966    1256    3668    4176 
   Size:        966     290    2412     508

  Fragments arranged by size: 

                2412     966     508     290
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1744    2346    3757    3829    4176 
   Size:       1744     602    1411      72     347

  Fragments arranged by size: 

                1744    1411     602     347      72
 
TatI w'GTAC_w

Cuts at:      0     573    1039    1393    2696    3424    4176 
   Size:        573     466     354    1303     728     752

  Fragments arranged by size: 

                1303     752     728     573     466     354
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2338    2370    3629    3661    4176 
   Size:       2338      32    1259      32     515

  Fragments arranged by size: 

                2338    1259     515      32      32
 
XbaI T'CTAG_A

Cuts at:      0    2614    4176 
   Size:       2614    1562
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2232    4176 
   Size:       2232    1944
 
XmnI GAAnn'nnTTC

Cuts at:      0    1220    2387    4176 
   Size:       1220    1167    1789

  Fragments arranged by size: 

                1789    1220    1167
 
ZraI GAC'GTC

Cuts at:      0     654    4176 
   Size:        654    3522
 

 Enzymes that do cut:

     AarI    AatII     AcuI   AflIII     AleI     AlfI     AloI    AlwNI
     ApaI    ApaLI     ApoI     AseI     BanI    BanII     BbsI    BbvCI
     BcgI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BssSI   BstAPI    BstBI    BstYI
   Bsu36I     BtgI     BtsI     ClaI     DraI     DrdI     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I
   HincII  HindIII     MmeI     MscI     MslI   MspA1I     NdeI     NheI
     PasI    PflMI     PpiI    PpuMI     PsiI   PspOMI     PsrI     PstI
    PvuII    SanDI     SapI     ScaI    SexAI     SfcI     SmlI     SpeI
     SspI     StyI    TaqII     TatI     TstI     XbaI     XcmI     XmnI
     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BciVI    BfrBI
     BglI    BsaAI    BsiEI    BsiWI    BspEI    BsrFI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I    BtgZI    CspCI   DraIII     EagI  EcoICRI
    EcoRV     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NcoI   NgoMIV     NotI     NruI     NsiI
     NspI     PacI     PciI     PfoI     PmeI     PmlI    PshAI    PspXI
     PvuI    RsrII     SacI    SacII     SalI     SbfI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     StuI     SwaI
  Tth111I     XhoI     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com