Restriction map for pF1KA1575
##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AcsI        AflII       AgeI        AhdI        Alw44I      
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AspI        BbrPI       
BclI        BfrI        BsaBI       BsaI        BsiWI       
BspHI       BsrBI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstZ17I     CpoI        DraI        
Eam1105I    EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HincII      HindII      HpaI        MamI        
MfeI        MluI        MseI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       PvuI        
RcaI        RsrII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmlI        SpeI        Sse8387I    SspI        SwaI        
Tru9I       Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII        1303  GACGTC               GACGT^C
Acc65I       2656  GGTACC               G^GTACC
AocI         1987  CCTGAGG              CC^T[ACGT]AGG
Asp718I      2656  GGTACC               G^GTACC
AviII         844  TGCGCA               TGC^GCA
AvrII        2673  CCTAGG               C^CTAGG
BalI         2493  TGGCCA               TGG^CCA
BamHI        2757  GGATCC               G^GATCC
BbeI         1846  GGCGCC               GGCGC^C
BbvCI         667  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1176  GTATCC               GTATCC(6/5)
BglI         2106  GCCCTATGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1492  AGATCT               A^GATCT
BlnI         2673  CCTAGG               C^CTAGG
BsmBI         408  CGTCTC               CGTCTC(1/5)
BsmI         1933  GCATTC               GAATGC(1/-1)
Bsp1407I      650  TGTACA               T^GTACA
BspDI         190  ATCGAT               AT^CGAT
BspLU11I      647  ACATGT               A^CATGT
BsrGI         650  TGTACA               T^GTACA
BstXI        1388  CCACGGTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1987  CCTGAGG              CC^T[ACGT]AGG
ClaI          190  ATCGAT               AT^CGAT
DrdI         2401  GACAAGCTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1987  CCTGAGG              CC^T[ACGT]AGG
FspI          844  TGCGCA               TGC^GCA
HindIII      2404  AAGCTT               A^AGCTT
KasI         1846  GGCGCC               G^GCGCC
KpnI         2656  GGTACC               GGTAC^C
KspI         1151  CCGCGG               CCGC^GG
MluNI        2493  TGGCCA               TGG^CCA
MscI         2493  TGGCCA               TGG^CCA
NaeI         2529  GCCGGC               GCC^GGC
NarI         1846  GGCGCC               GG^CGCC
NgoMIV       2529  GCCGGC               G^CCGGC
PshAI        2385  GACTGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII          36  CAGCTG               CAG^CTG
SacI         1755  GAGCTC               GAGCT^C
SacII        1151  CCGCGG               CCGC^GG
SfoI         1846  GGCGCC               GGC^GCC
SnaBI        2413  TACGTA               TAC^GTA
SphI         1224  GCATGC               GCATG^C
SspBI         650  TGTACA               T^GTACA
Tsp509I      1950  AATT                 ^AATT
XcmI          183  CCATAGGATCGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII        1152  CGCG                 CG^CG
             1634  CGCG                
AclI           28  AACGTT               AA^CGTT
             1666  AACGTT              
AfaI          651  GTAC                 GT^AC
             2657  GTAC                
AflIII        647  ACATGT               A^C[GA][CT]GT
             2459  ACGTGT              
AlwNI         691  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1982  CAGCCCCTG           
BcgI         2721  CGATGGCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2946  CGAACTGTATGC        
BlpI         2503  GCTGAGC              GC^T[ACGT]AGC
             2970  GCTCAGC             
Bpu1102I     2503  GCTGAGC              GC^T[ACGT]AGC
             2970  GCTCAGC             
BsaAI        2413  TACGTA               [CT]AC^GT[GA]
             2914  TACGTG              
BsgI          711  CTGCAC               GTGCAG(16/14)
             1688  GTGCAG              
BsiEI        1012  CGGCCG               CG[GA][CT]^CG
             2124  CGGCCG              
BsrFI        2122  ACCGGC               [GA]^CCGG[CT]
             2529  GCCGGC              
BstEII        960  GGTCACC              G^GT[ACGT]ACC
             2920  GGTGACC             
BstPI         960  GGTCACC              G^GT[ACGT]ACC
             2920  GGTGACC             
BstUI        1152  CGCG                 CG^CG
             1634  CGCG                
CelII        2503  GCTGAGC              GC^T[ACGT]AGC
             2970  GCTCAGC             
Cfr10I       2122  ACCGGC               [GA]^CCGG[CT]
             2529  GCCGGC              
DraIII        157  CACAACGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2789  CACCAAGTG           
EagI         1012  CGGCCG               C^GGCCG
             2124  CGGCCG              
EarI          139  GAAGAG               CTCTTC(1/4)
             1552  GAAGAG              
EclXI        1012  CGGCCG               C^GGCCG
             2124  CGGCCG              
Eco52I       1012  CGGCCG               C^GGCCG
             2124  CGGCCG              
EcoNI        1955  CCTACCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2562  CCTGAGAGAGG         
EcoO65I       960  GGTCACC              G^GT[ACGT]ACC
             2920  GGTGACC             
HgaI          109  GACGC                GACGC(5/10)
              875  GCGTC               
Ksp632I       139  GAAGAG               CTCTTC(1/4)
             1552  GAAGAG              
MslI         2326  CATCCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2454  CACAAACGTG          
MvnI         1152  CGCG                 CG^CG
             1634  CGCG                
Psp1406I       28  AACGTT               AA^CGTT
             1666  AACGTT              
PstI         1049  CTGCAG               CTGCA^G
             2536  CTGCAG              
RsaI          651  GTAC                 GT^AC
             2657  GTAC                
StuI          508  AGGCCT               AGG^CCT
              656  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AccI        AccIII      AciI        AcyI        AluI        
AlwI        Aor51HI     AspHI       AvaI        AvaII       
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaHI       
BsaJI       BsaWI       BseAI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspEI       BspMI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        Eco47III    EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MroI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
StyI        TaqI        TfiI        TseI        Tsp45I      
TspRI       TthHB8I     Van91I      XhoII       XmaCI       
XmaI        
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