Restriction map for pF1KA1492
 (Linear) (Six-base) MAPSORT of: KIAA1492.seq  Check: 3941  from: 1  to: 2367



KIAA1492.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AccI GT'mk_AC

Cuts at:      0     436    2237    2367 
   Size:        436    1801     130

  Fragments arranged by size: 

                1801     436     130
 
Acc65I G'GTAC_C

Cuts at:      0     759    2367 
   Size:        759    1608
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1370    1561    1576    2292    2367 
   Size:       1370     191      15     716      75

  Fragments arranged by size: 

                1370     716     191      75      15
 
AflIII A'CryG_T

Cuts at:      0     282    2367 
   Size:        282    2085
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     625    1552    2367 
   Size:        625     927     815

  Fragments arranged by size: 

                 927     815     625
 
AlwNI CAG_nnn'CTG

Cuts at:      0     552    1144    1803    2367 
   Size:        552     592     659     564

  Fragments arranged by size: 

                 659     592     564     552
 
ApoI r'AATT_y

Cuts at:      0     165    1126    1168    1289    1808    1873    2302    2367 
   Size:        165     961      42     121     519      65     429      65

  Fragments arranged by size: 

                 961     519     429     165     121      65      65      42
            
 
AseI AT'TA_AT

Cuts at:      0    2137    2191    2367 
   Size:       2137      54     176

  Fragments arranged by size: 

                2137     176      54
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     242     275    2367 
   Size:        242      33    2092

  Fragments arranged by size: 

                2092     242      33
 
BamHI G'GATC_C

Cuts at:      0    1990    2367 
   Size:       1990     377
 
BanI G'GyrC_C

Cuts at:      0     759    2367 
   Size:        759    1608
 
BanII G_rGCy'C

Cuts at:      0     902    1103    2367 
   Size:        902     201    1264

  Fragments arranged by size: 

                1264     902     201
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     200    2367 
   Size:        200    2167
 
BbvCI CC'TCA_GC

Cuts at:      0    2031    2367 
   Size:       2031     336
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     809     827     833    2367 
   Size:        809      18       6    1534

  Fragments arranged by size: 

                1534     809      18       6
 
BfrBI ATG'CAT

Cuts at:      0    1373    2367 
   Size:       1373     994
 
BglII A'GATC_T

Cuts at:      0    1259    2367 
   Size:       1259    1108
 
BmgBI CAC'GTC

Cuts at:      0    1162    2367 
   Size:       1162    1205
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      52    1254    1468    1737    2367 
   Size:         52    1202     214     269     630

  Fragments arranged by size: 

                1202     630     269     214      52
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479     809    2234    2367 
   Size:        479     330    1425     133

  Fragments arranged by size: 

                1425     479     330     133
 
Bpu10I CC'TnA_GC

Cuts at:      0    2031    2367 
   Size:       2031     336
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1021    2367 
   Size:       1021    1346
 
BsaAI yAC'GTr

Cuts at:      0    1076    2367 
   Size:       1076    1291
 
BsaBI GATnn'nnATC

Cuts at:      0    1258    2367 
   Size:       1258    1109
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1090    1120    1885    1915    2367 
   Size:       1090      30     765      30     452

  Fragments arranged by size: 

                1090     765     452      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1111    2367 
   Size:       1111    1256
 
BseYI C'CCAG_C

Cuts at:      0    1503    2367 
   Size:       1503     864
 
BsiHKAI G_wGCw'C

Cuts at:      0     902    1306    2367 
   Size:        902     404    1061

  Fragments arranged by size: 

                1061     902     404
 
BsmI GAATG_Cn'

Cuts at:      0     687    2367 
   Size:        687    1680
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1109    2367 
   Size:       1109    1258
 
Bsp1286I G_dGCh'C

Cuts at:      0     902    1103    1306    2367 
   Size:        902     201     203    1061

  Fragments arranged by size: 

                1061     902     203     201
 
BspHI T'CATG_A

Cuts at:      0    1388    2367 
   Size:       1388     979
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     823    2223    2367 
   Size:        823    1400     144

  Fragments arranged by size: 

                1400     823     144
 
BsrDI GCAATG_nn'

Cuts at:      0     607    2367 
   Size:        607    1760
 
BsrGI T'GTAC_A

Cuts at:      0    1334    1402    2367 
   Size:       1334      68     965

  Fragments arranged by size: 

                1334     965      68
 
BstEII G'GTnAC_C

Cuts at:      0     710    2367 
   Size:        710    1657
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     975    2367 
   Size:        975    1392
 
BstYI r'GATC_y

Cuts at:      0    1259    1990    2367 
   Size:       1259     731     377

  Fragments arranged by size: 

                1259     731     377
 
BstZ17I GTA'TAC

Cuts at:      0     437    2367 
   Size:        437    1930
 
Bsu36I CC'TnA_GG

Cuts at:      0     827    2367 
   Size:        827    1540
 
BtgI C'CryG_G

Cuts at:      0      18     767    1994    2367 
   Size:         18     749    1227     373

  Fragments arranged by size: 

                1227     749     373      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     681    2367 
   Size:        681    1686
 
DraI TTT'AAA

Cuts at:      0     386     921    1983    2367 
   Size:        386     535    1062     384

  Fragments arranged by size: 

                1062     535     386     384
 
EarI CTCTTCn'nnn_

Cuts at:      0     668    1670    2263    2367 
   Size:        668    1002     593     104

  Fragments arranged by size: 

                1002     668     593     104
 
EcoICRI GAG'CTC

Cuts at:      0     900    2367 
   Size:        900    1467
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479     809    1370    1561    1576    2234    2292    2367 
   Size:        479     330     561     191      15     658      58      75

  Fragments arranged by size: 

                 658     561     479     330     191      75      58      15
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     830    2367 
   Size:        830    1537
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1470    2367 
   Size:       1470     897
 
FspI TGC'GCA

Cuts at:      0       4    2367 
   Size:          4    2363
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1051    1083    2262    2294    2367 
   Size:       1051      32    1179      32      73

  Fragments arranged by size: 

                1179    1051      73      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1975    2367 
   Size:       1975     392
 
KpnI G_GTAC'C

Cuts at:      0     763    2367 
   Size:        763    1604
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2199    2367 
   Size:       2199     168
 
MslI CAynn'nnrTG

Cuts at:      0     127    1183    1747    2367 
   Size:        127    1056     564     620

  Fragments arranged by size: 

                1056     620     564     127
 
MspA1I CmG'CkG

Cuts at:      0      20     552    1332    1701    1867    2367 
   Size:         20     532     780     369     166     500

  Fragments arranged by size: 

                 780     532     500     369     166      20
 
NcoI C'CATG_G

Cuts at:      0     767    2367 
   Size:        767    1600
 
NdeI CA'TA_TG

Cuts at:      0     405     447    2367 
   Size:        405      42    1920

  Fragments arranged by size: 

                1920     405      42
 
NsiI A_TGCA'T

Cuts at:      0    1375    2367 
   Size:       1375     992
 
NspI r_CATG'y

Cuts at:      0     286    2367 
   Size:        286    2081
 
PciI A'CATG_T

Cuts at:      0     282    2367 
   Size:        282    2085
 
PpuMI rG'GwC_Cy

Cuts at:      0    1470    2367 
   Size:       1470     897
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1601    1633    2367 
   Size:       1601      32     734

  Fragments arranged by size: 

                1601     734      32
 
PvuII CAG'CTG

Cuts at:      0     552    1332    1701    1867    2367 
   Size:        552     780     369     166     500

  Fragments arranged by size: 

                 780     552     500     369     166
 
SacI G_AGCT'C

Cuts at:      0     902    2367 
   Size:        902    1465
 
SacII CC_GC'GG

Cuts at:      0      21    2367 
   Size:         21    2346
 
SanDI GG'GwC_CC

Cuts at:      0    1470    2367 
   Size:       1470     897
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1670    2263    2367 
   Size:       1670     593     104

  Fragments arranged by size: 

                1670     593     104
 
SfcI C'TryA_G

Cuts at:      0    1034    2286    2367 
   Size:       1034    1252      81

  Fragments arranged by size: 

                1252    1034      81
 
SspI AAT'ATT

Cuts at:      0    1513    2367 
   Size:       1513     854
 
StyI C'CwwG_G

Cuts at:      0     540     767    1465    2052    2367 
   Size:        540     227     698     587     315

  Fragments arranged by size: 

                 698     587     540     315     227
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1664    2367 
   Size:       1664     703
 
TatI w'GTAC_w

Cuts at:      0    1334    1402    2367 
   Size:       1334      68     965

  Fragments arranged by size: 

                1334     965      68
 
Tth111I GACn'n_nGTC

Cuts at:      0     511    2367 
   Size:        511    1856
 
XbaI T'CTAG_A

Cuts at:      0    1111    2367 
   Size:       1111    1256
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      68     186     236     324    1641    2367 
   Size:         68     118      50      88    1317     726

  Fragments arranged by size: 

                1317     726     118      88      68      50
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII     AlfI    AlwNI     ApoI     AseI
     BaeI    BamHI     BanI    BanII     BbsI    BbvCI    BciVI    BfrBI
    BglII    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaXI    BseRI    BseYI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI    BsrDI    BsrGI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     DraI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FspI    Hin4I  HindIII     KpnI     MmeI     MslI   MspA1I     NcoI
     NdeI     NsiI     NspI     PciI    PpuMI     PsrI    PvuII     SacI
    SacII    SanDI     SapI     SfcI     SspI     StyI    TaqII     TatI
  Tth111I     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BbeI
     BcgI     BclI     BglI     BlpI Bme1580I     BmtI     BplI    BpuEI
    BsaHI    BsaWI     BsgI    BsiEI    BsiWI    BspEI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI    BstBI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI    EcoRI    EcoRV     FalI     FseI
    FspAI    HaeII   HincII     HpaI     KasI     MfeI     MluI     MscI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PstI     PvuI    RsrII     SalI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI     TstI     XhoI     XmaI     XmnI
     ZraI
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