Restriction map for
pF1KA1489
(Linear) (Six-base) MAPSORT of: KIAA1489.seq Check: 9610 from: 1 to: 1869
KIAA1489.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AclI AA'CG_TT
Cuts at: 0 354 1869
Size: 354 1515
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 809 1003 1014 1370 1469 1869
Size: 411 398 194 11 356 99 400
Fragments arranged by size:
411 400 398 356 194 99 11
AflIII A'CryG_T
Cuts at: 0 151 500 1707 1869
Size: 151 349 1207 162
Fragments arranged by size:
1207 349 162 151
AgeI A'CCGG_T
Cuts at: 0 1676 1869
Size: 1676 193
AhdI GACnn_n'nnGTC
Cuts at: 0 1680 1869
Size: 1680 189
AleI CACnn'nnGTG
Cuts at: 0 484 1869
Size: 484 1385
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1280 1869
Size: 1280 589
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 460 492 1869
Size: 460 32 1377
Fragments arranged by size:
1377 460 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1492 1869
Size: 1492 377
ApoI r'AATT_y
Cuts at: 0 121 1409 1499 1593 1869
Size: 121 1288 90 94 276
Fragments arranged by size:
1288 276 121 94 90
BbsI GAAGACnn'nnnn_
Cuts at: 0 632 1475 1869
Size: 632 843 394
Fragments arranged by size:
843 632 394
BfrBI ATG'CAT
Cuts at: 0 265 1869
Size: 265 1604
Bme1580I G_kGCm'C
Cuts at: 0 118 1869
Size: 118 1751
BmrI ACTGGGnnnn_n'
Cuts at: 0 624 1869
Size: 624 1245
BsaI GGTCTCn'nnnn_
Cuts at: 0 1688 1869
Size: 1688 181
BsaWI w'CCGG_w
Cuts at: 0 1676 1869
Size: 1676 193
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 460 490 1151 1181 1835 1865 1869
Size: 460 30 661 30 654 30 4
Fragments arranged by size:
661 654 460 30 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1172 1869
Size: 1172 697
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1286 1869
Size: 1286 583
BsiHKAI G_wGCw'C
Cuts at: 0 473 1869
Size: 473 1396
Bsp1286I G_dGCh'C
Cuts at: 0 118 473 1869
Size: 118 355 1396
Fragments arranged by size:
1396 355 118
BspHI T'CATG_A
Cuts at: 0 1281 1869
Size: 1281 588
BsrDI GCAATG_nn'
Cuts at: 0 290 1264 1869
Size: 290 974 605
Fragments arranged by size:
974 605 290
BsrFI r'CCGG_y
Cuts at: 0 1676 1869
Size: 1676 193
BsrGI T'GTAC_A
Cuts at: 0 213 1869
Size: 213 1656
Bsu36I CC'TnA_GG
Cuts at: 0 220 1869
Size: 220 1649
BtgI C'CryG_G
Cuts at: 0 1783 1869
Size: 1783 86
BtsI GCAGTG_nn'
Cuts at: 0 479 1869
Size: 479 1390
DraI TTT'AAA
Cuts at: 0 557 1869
Size: 557 1312
DrdI GACnn_nn'nnGTC
Cuts at: 0 1374 1869
Size: 1374 495
EaeI y'GGCC_r
Cuts at: 0 1355 1869
Size: 1355 514
EarI CTCTTCn'nnn_
Cuts at: 0 430 855 1769 1820 1869
Size: 430 425 914 51 49
Fragments arranged by size:
914 430 425 51 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 809 1003 1014 1370 1469 1869
Size: 411 398 194 11 356 99 400
Fragments arranged by size:
411 400 398 356 194 99 11
EcoO109I rG'GnC_Cy
Cuts at: 0 1376 1869
Size: 1376 493
EcoRI G'AATT_C
Cuts at: 0 121 1869
Size: 121 1748
EcoRV GAT'ATC
Cuts at: 0 951 1869
Size: 951 918
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1666 1698 1869
Size: 1666 32 171
Fragments arranged by size:
1666 171 32
MfeI C'AATT_G
Cuts at: 0 1151 1869
Size: 1151 718
MluI A'CGCG_T
Cuts at: 0 500 1869
Size: 500 1369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 1869
Size: 32 1837
MscI TGG'CCA
Cuts at: 0 1357 1869
Size: 1357 512
MslI CAynn'nnrTG
Cuts at: 0 178 484 1294 1628 1869
Size: 178 306 810 334 241
Fragments arranged by size:
810 334 306 241 178
MspA1I CmG'CkG
Cuts at: 0 513 907 1390 1869
Size: 513 394 483 479
Fragments arranged by size:
513 483 479 394
NcoI C'CATG_G
Cuts at: 0 1783 1869
Size: 1783 86
NsiI A_TGCA'T
Cuts at: 0 267 1869
Size: 267 1602
NspI r_CATG'y
Cuts at: 0 155 1869
Size: 155 1714
PacI TTA_AT'TAA
Cuts at: 0 374 1869
Size: 374 1495
PciI A'CATG_T
Cuts at: 0 151 1869
Size: 151 1718
PflMI CCAn_nnn'nTGG
Cuts at: 0 781 1869
Size: 781 1088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 460 492 1869
Size: 460 32 1377
Fragments arranged by size:
1377 460 32
PpuMI rG'GwC_Cy
Cuts at: 0 1376 1869
Size: 1376 493
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1300 1332 1869
Size: 1300 32 537
Fragments arranged by size:
1300 537 32
PstI C_TGCA'G
Cuts at: 0 1395 1869
Size: 1395 474
PvuII CAG'CTG
Cuts at: 0 513 907 1390 1869
Size: 513 394 483 479
Fragments arranged by size:
513 483 479 394
SexAI A'CCwGG_T
Cuts at: 0 1075 1869
Size: 1075 794
SfcI C'TryA_G
Cuts at: 0 299 331 1391 1472 1869
Size: 299 32 1060 81 397
Fragments arranged by size:
1060 397 299 81 32
SmlI C'TyrA_G
Cuts at: 0 8 1869
Size: 8 1861
SpeI A'CTAG_T
Cuts at: 0 1369 1869
Size: 1369 500
SspI AAT'ATT
Cuts at: 0 367 1869
Size: 367 1502
StyI C'CwwG_G
Cuts at: 0 1330 1783 1869
Size: 1330 453 86
Fragments arranged by size:
1330 453 86
SwaI ATTT'AAAT
Cuts at: 0 557 1869
Size: 557 1312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 729 1869
Size: 729 1140
TatI w'GTAC_w
Cuts at: 0 213 1316 1710 1869
Size: 213 1103 394 159
Fragments arranged by size:
1103 394 213 159
XmnI GAAnn'nnTTC
Cuts at: 0 578 1869
Size: 578 1291
Enzymes that do cut:
AclI AcuI AflIII AgeI AhdI AleI AlfI AloI
AlwNI ApoI BbsI BfrBI Bme1580I BmrI BsaI BsaWI
BsaXI BseRI BsgI BsiHKAI Bsp1286I BspHI BsrDI BsrFI
BsrGI Bsu36I BtgI BtsI DraI DrdI EaeI EarI
Eco57MI EcoO109I EcoRI EcoRV Hin4I MfeI MluI MmeI
MscI MslI MspA1I NcoI NsiI NspI PacI PciI
PflMI PpiI PpuMI PsrI PstI PvuII SexAI SfcI
SmlI SpeI SspI StyI SwaI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaHI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgZI ClaI CspCI DraIII EagI EciI
EcoICRI EcoNI FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI NaeI NarI NdeI NgoMIV
NheI NotI NruI PasI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI StuI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI