Restriction map for pF1KA1478
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp718I     AspI        AvaI        AviII       BalI        
BamHI       BanI        BbeI        BbrPI       BcgI        
BclI        BfrI        BglII       BlpI        Bpu1102I    
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspEI       BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EaeI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FbaI        FseI        FspI        
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          622  GTAGAC               GT^[AC][GT]AC
AflIII       1659  ACATGT               A^C[GA][CT]GT
AhdI         1057  GACTTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI          682  GGGCCC               GGGCC^C
AspEI        1057  GACTTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         197  GTGCTC               G[AT]GC[AT]^C
AvrII        1854  CCTAGG               C^CTAGG
BanII         682  GGGCCC               G[GA]GC[CT]^C
BciVI           3  GGATAC               GTATCC(6/5)
BcnI           51  CCGGG                CC^[GC]GG
BglI         1480  GCCAATCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1854  CCTAGG               C^CTAGG
BsaAI         224  CACGTA               [CT]AC^GT[GA]
BsaI          963  GGTCTC               GGTCTC(1/5)
BsiHKAI       197  GTGCTC               G[AT]GC[AT]^C
BspLU11I     1659  ACATGT               A^CATGT
BsrDI         407  GCAATG               GCAATG(2/0)
BstAPI         39  GCAGCTTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1277  TTCGAA               TT^CGAA
BstXI        1377  CCAAGCTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII        1725  GGGACCT              [GA]G^G[ACGT]CC[CT]
Eam1105I     1057  GACTTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     1725  GGGACCT              [GA]G^G[ACGT]CC[CT]
FauI          680  GCGGG                CCCGC(4/6)
HapII          51  CCGG                 C^CGG
HgaI         1566  GCGTC                GACGC(5/10)
HincII       1568  GTCAAC               GT[CT]^[GA]AC
HindII       1568  GTCAAC               GT[CT]^[GA]AC
HpaII          51  CCGG                 C^CGG
MaeII         225  ACGT                 A^CGT
MspI           51  CCGG                 C^CGG
NciI           51  CCGGG                CC^[GC]GG
NspV         1277  TTCGAA               TT^CGAA
PflMI         144  CCATGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1725  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI         985  GACCGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          617  CTGCAG               CTGCA^G
SfuI         1277  TTCGAA               TT^CGAA
SphI          219  GCATGC               GCATG^C
StuI         1143  AGGCCT               AGG^CCT
Van91I        144  CCATGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcsI          922  AAATTT               [GA]^AATT[CT]
             1195  AAATTC              
AlwNI         182  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
              659  CAGTGACTG           
ApoI          922  AAATTT               [GA]^AATT[CT]
             1195  AAATTC              
Asp700I       516  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1291  GAACCTCTTC          
BbsI          516  GAAGAC               GAAGAC(2/6)
             1351  GAAGAC              
BbvCI        1224  CCTCAGC              CCTCAGC(-5/-2)
              265  GCTGAGG             
BpmI          796  CTGGAG               CTGGAG(16/14)
             1606  CTGGAG              
BpuAI         516  GAAGAC               GAAGAC(2/6)
             1351  GAAGAC              
BseRI        1222  CTCCTC               GAGGAG(10/8)
              355  GAGGAG              
BspHI        1427  TCATGA               T^CATGA
             1625  TCATGA              
BstYI         165  GGATCT               [GA]^GATC[CT]
             1154  GGATCT              
EcoRV         463  GATATC               GAT^ATC
             1240  GATATC              
HaeII         548  GGCGCT               [GA]GCGC^[CT]
             1586  AGCGCC              
MflI          165  GGATCT               [GA]^GATC[CT]
             1154  GGATCT              
MslI         1092  CATTGTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1626  CATGATGGTG          
NspI          219  GCATGC               [GA]CATG^[CT]
             1659  ACATGT              
PvuII         301  CAGCTG               CAG^CTG
              793  CAGCTG              
RcaI         1427  TCATGA               T^CATGA
             1625  TCATGA              
SmlI         1756  CTCAAG               C^T[CT][GA]AG
             1891  CTCAAG              
XhoII         165  GGATCT               [GA]^GATC[CT]
             1154  GGATCT              
XmnI          516  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1291  GAACCTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BbvI        BfaI        BmyI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MnlI        MseI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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