Restriction map for pF1KA1477
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BciVI       BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BsiEI       BsiWI       BsmI        BspLU11I    BssHII      
Bst1107I    BstAPI      BstBI       BstXI       BstYI       
BstZ17I     CelII       CfoI        CpoI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HaeII       HhaI        HinP1I      HpaI        KasI        
KpnI        KspI        MamI        MfeI        MflI        
MluI        MslI        MunI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SphI        
Sse8387I    StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   67 enzymes) #
###################################

AccI          299  GTCTAC               GT^[AC][GT]AC
AccII        1979  CGCG                 CG^CG
AccIII        915  TCCGGA               T^CCGGA
AflII         481  CTTAAG               C^TTAAG
AflIII        234  ACACGT               A^C[GA][CT]GT
AocI         1252  CCTCAGG              CC^T[ACGT]AGG
AspHI        1755  GAGCTC               G[AT]GC[AT]^C
BalI          708  TGGCCA               TGG^CCA
BanII        1755  GAGCTC               G[GA]GC[CT]^C
BbsI         2007  GAAGAC               GAAGAC(2/6)
BbvCI        1568  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1344  CGAGATGACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1203  TGATCA               T^GATCA
BfrI          481  CTTAAG               C^TTAAG
BmyI         1755  GAGCTC               G[AGT]GC[ACT]^C
BpuAI        2007  GAAGAC               GAAGAC(2/6)
BsaAI        1474  TACGTA               [CT]AC^GT[GA]
BseAI         915  TCCGGA               T^CCGGA
BseRI        1285  GAGGAG               GAGGAG(10/8)
BsgI         2146  GTGCAG               GTGCAG(16/14)
BsiHKAI      1755  GAGCTC               G[AT]GC[AT]^C
BsmBI        2123  GAGACG               CGTCTC(1/5)
Bsp1286I     1755  GAGCTC               G[AGT]GC[ACT]^C
Bsp1407I      441  TGTACA               T^GTACA
BspEI         915  TCCGGA               T^CCGGA
BspHI         876  TCATGA               T^CATGA
BspMI        1824  ACCTGC               ACCTGC(4/8)
BsrBI          34  GAGCGG               CCGCTC(-3/-3)
BsrFI        1661  ACCGGC               [GA]^CCGG[CT]
BsrGI         441  TGTACA               T^GTACA
BstEII        951  GGTCACC              G^GT[ACGT]ACC
BstNI        1666  CCTGG                CC^[AT]GG
BstPI         951  GGTCACC              G^GT[ACGT]ACC
BstUI        1979  CGCG                 CG^CG
Bsu36I       1252  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       1661  ACCGGC               [GA]^CCGG[CT]
DraI          717  TTTAAA               TTT^AAA
DraII        1099  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DrdI          943  GACATTATGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          708  TGGCCA               [CT]^GGCC[GA]
Eco81I       1252  CCTCAGG              CC^T[ACGT]AGG
EcoO109I     1099  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       951  GGTCACC              G^GT[ACGT]ACC
EcoRII       1666  CCTGG                ^CC[AT]GG
FbaI         1203  TGATCA               T^GATCA
HincII       1878  GTCAAC               GT[CT]^[GA]AC
HindII       1878  GTCAAC               GT[CT]^[GA]AC
HindIII      1655  AAGCTT               A^AGCTT
MluNI         708  TGGCCA               TGG^CCA
MroI          915  TCCGGA               T^CCGGA
MscI          708  TGGCCA               TGG^CCA
MspA1I       1189  CAGCGG               C[AC]G^C[GT]G
MvaI         1666  CCTGG                CC^[AT]GG
MvnI         1979  CGCG                 CG^CG
NdeI         1445  CATATG               CA^TATG
NspI         1860  ACATGC               [GA]CATG^[CT]
PpuMI        1099  AGGTCCC              [GA]G^G[AT]CC[CT]
PstI         1538  CTGCAG               CTGCA^G
RcaI          876  TCATGA               T^CATGA
SacI         1755  GAGCTC               GAGCT^C
SapI         1544  GCTCTTC              GCTCTTC(1/4)
ScaI         1717  AGTACT               AGT^ACT
SfcI         1538  CTGCAG               C^T[GA][CT]AG
SnaBI        1474  TACGTA               TAC^GTA
SpeI         2014  ACTAGT               A^CTAGT
SspBI         441  TGTACA               T^GTACA
SspI          517  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcyI           12  GACGCC               G[GA]^CG[CT]C
              664  GGCGTC              
AlwI          913  GATCC                GGATC(4/5)
             1333  GGATC               
Asp700I       490  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              787  GAAAATCTTC          
AvaI          607  CCCGAG               C^[CT]CG[GA]G
             1748  CCCGAG              
BpmI         1727  CTCCAG               CTGGAG(16/14)
             1418  CTGGAG              
BsaHI          12  GACGCC               G[GA]^CG[CT]C
              664  GGCGTC              
BsaWI         915  TCCGGA               [AT]^CCGG[AT]
             1733  ACCGGA              
BsmAI        1949  GAGAC                GTCTC(1/5)
             2123  GAGAC               
BsoBI         607  CCCGAG               C^[CT]CG[GA]G
             1748  CCCGAG              
BspDI         857  ATCGAT               AT^CGAT
             1469  ATCGAT              
BssSI         968  CACGAG               CACGAG(-5/-1)
             2143  CTCGTG              
ClaI          857  ATCGAT               AT^CGAT
             1469  ATCGAT              
EcoNI         349  CCTAATATAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1360  CCTCTTGTAGG         
FauI         1580  CCCGC                CCCGC(4/6)
               36  GCGGG               
Hin1I          12  GACGCC               G[GA]^CG[CT]C
              664  GGCGTC              
MwoI         1788  GCTCCGGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1980  GCGTTCTTTGC         
PleI         1725  GACTC                GAGTC(4/5)
              657  GAGTC               
SmlI          481  CTTAAG               C^T[CT][GA]AG
             1758  CTCAAG              
XcmI          215  CCAAAGTCAAATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2012  CCACTAGTTCAATGG     
XmnI          490  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              787  GAAAATCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaII       BbvI        BcnI        BfaI        BsaJI       
BsiYI       BslI        BsmFI       BsrDI       BsrI        
BssKI       BstF5I      Cac8I       Cfr13I      DdeI        
DpnII       DsaI        EarI        EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HgaI        HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MspI        NciI        NcoI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com