Restriction map for pF1KA1469
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BbvCI       BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaHI       BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BsoBI       BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstUI       BstXI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO109I    EcoRV       FseI        
FspI        HaeII       HgaI        Hin1I       HpaI        
KasI        KpnI        KspI        MluI        MroI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NspI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SspI        StuI        SwaI        Tth111I     
Van91I      XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AlwNI        1727  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I      1012  GAAAAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         1141  TGGCCA               TGG^CCA
BanI          783  GGTGCC               G^G[CT][GA]CC
BanII         991  GGGCTC               G[GA]GC[CT]^C
BcgI          813  GCAGCTCTATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1122  GTATCC               GTATCC(6/5)
BclI            2  TGATCA               T^GATCA
BcnI         1338  CCCGG                CC^[GC]GG
BsaAI         947  TACGTG               [CT]AC^GT[GA]
BsaBI         547  GATGATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          589  GGTCTC               GGTCTC(1/5)
BsmI          445  GCATTC               GAATGC(1/-1)
BstBI        1511  TTCGAA               TT^CGAA
BstEII       1617  GGTTACC              G^GT[ACGT]ACC
BstPI        1617  GGTTACC              G^GT[ACGT]ACC
CfoI            8  GCGC                 GCG^C
DsaI         1442  CCATGG               C^C[GA][CT]GG
EaeI         1141  TGGCCA               [CT]^GGCC[GA]
EcoNI        1126  CCTGCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1617  GGTTACC              G^GT[ACGT]ACC
EcoRI         702  GAATTC               G^AATTC
EcoT22I      1431  ATGCAT               ATGCA^T
FbaI            2  TGATCA               T^GATCA
HapII        1339  CCGG                 C^CGG
HhaI            8  GCGC                 GCG^C
HinP1I          8  GCGC                 G^CGC
HincII        979  GTCAAC               GT[CT]^[GA]AC
HindII        979  GTCAAC               GT[CT]^[GA]AC
HindIII       751  AAGCTT               A^AGCTT
HpaII        1339  CCGG                 C^CGG
MamI          547  GATGATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1364  CAATTG               C^AATTG
MluNI        1141  TGGCCA               TGG^CCA
MscI         1141  TGGCCA               TGG^CCA
MspA1I       1314  CAGCTG               C[AC]G^C[GT]G
MspI         1339  CCGG                 C^CGG
MunI         1364  CAATTG               C^AATTG
MwoI         1627  GCCCTTCTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI         1338  CCCGG                CC^[GC]GG
NcoI         1442  CCATGG               C^CATGG
NsiI         1431  ATGCAT               ATGCA^T
NspV         1511  TTCGAA               TT^CGAA
PstI          422  CTGCAG               CTGCA^G
PvuII        1314  CAGCTG               CAG^CTG
SapI         1671  GCTCTTC              GCTCTTC(1/4)
ScaI         1388  AGTACT               AGT^ACT
SfuI         1511  TTCGAA               TT^CGAA
SpeI          595  ACTAGT               A^CTAGT
XbaI          615  TCTAGA               T^CTAGA
XcmI         1574  CCAATTTACCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1012  GAAAAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AocI          744  CCTGAGG              CC^T[ACGT]AGG
              859  CCTCAGG             
BmyI          784  GTGCCC               G[AGT]GC[ACT]^C
              991  GGGCTC              
BseRI        1704  GAGGAG               GAGGAG(10/8)
             1819  GAGGAG              
BsgI          716  CTGCAC               GTGCAG(16/14)
             1502  CTGCAC              
Bsp1286I      784  GTGCCC               G[AGT]GC[ACT]^C
              991  GGGCTC              
Bsp1407I     1517  TGTACA               T^GTACA
             1865  TGTACA              
BsrDI        1623  CATTGC               GCAATG(2/0)
             1799  GCAATG              
BsrGI        1517  TGTACA               T^GTACA
             1865  TGTACA              
BstYI        1038  GGATCT               [GA]^GATC[CT]
             1348  GGATCT              
Bsu36I        744  CCTGAGG              CC^T[ACGT]AGG
              859  CCTCAGG             
Cfr13I       1329  GGGCC                G^G[ACGT]CC
             1611  GGCCC               
DrdI          409  GACAACTCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              681  GACAGAGCTGTC        
Eco81I        744  CCTGAGG              CC^T[ACGT]AGG
              859  CCTCAGG             
EcoT14I      1131  CCAAGG               C^C[AT][AT]GG
             1442  CCATGG              
FauI          115  GCGGG                CCCGC(4/6)
              926  GCGGG               
MaeII         948  ACGT                 A^CGT
              983  ACGT                
MflI         1038  GGATCT               [GA]^GATC[CT]
             1348  GGATCT              
Sau96I       1329  GGGCC                G^G[ACGT]CC
             1611  GGCCC               
SmlI         1218  CTCAAG               C^T[CT][GA]AG
             1677  CTCAAG              
SspBI        1517  TGTACA               T^GTACA
             1865  TGTACA              
StyI         1131  CCAAGG               C^C[AT][AT]GG
             1442  CCATGG              
XhoII        1038  GGATCT               [GA]^GATC[CT]
             1348  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BbvI        BfaI        BpmI        BsaJI       
BsiYI       BslI        BsmAI       BsmFI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       DdeI        
DpnII       EarI        EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MslI        MvaI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      ScrFI       
SfaNI       SfcI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
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