Restriction map for pF1KA1469
 (Linear) (Six-base) MAPSORT of: KIAA1469.seq  Check: 4998  from: 1  to: 1947



KIAA1469.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     212    1947 
   Size:        178      34    1735

  Fragments arranged by size: 

                1735     178      34
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1732    1947 
   Size:       1732     215
 
ApoI r'AATT_y

Cuts at:      0     289     377     702    1947 
   Size:        289      88     325    1245

  Fragments arranged by size: 

                1245     325     289      88
 
BanI G'GyrC_C

Cuts at:      0     783    1947 
   Size:        783    1164
 
BanII G_rGCy'C

Cuts at:      0     995    1947 
   Size:        995     952
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     802     836    1947 
   Size:        802      34    1111

  Fragments arranged by size: 

                1111     802      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1133    1947 
   Size:       1133     814
 
BclI T'GATC_A

Cuts at:      0       2    1947 
   Size:          2    1945
 
BfrBI ATG'CAT

Cuts at:      0    1433    1947 
   Size:       1433     514
 
Bme1580I G_kGCm'C

Cuts at:      0     788    1947 
   Size:        788    1159
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1336    1947 
   Size:       1336     611
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      36    1133    1426    1947 
   Size:         36    1097     293     521

  Fragments arranged by size: 

                1097     521     293      36
 
Bpu10I CC'TnA_GC

Cuts at:      0     496    1947 
   Size:        496    1451
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1203    1662    1947 
   Size:       1203     459     285

  Fragments arranged by size: 

                1203     459     285
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     595    1947 
   Size:        595    1352
 
BsaAI yAC'GTr

Cuts at:      0     949    1947 
   Size:        949     998
 
BsaBI GATnn'nnATC

Cuts at:      0     551    1947 
   Size:        551    1396
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1719    1834    1947 
   Size:       1719     115     113

  Fragments arranged by size: 

                1719     115     113
 
BseYI C'CCAG_C

Cuts at:      0     217    1947 
   Size:        217    1730
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     701    1487    1947 
   Size:        701     786     460

  Fragments arranged by size: 

                 786     701     460
 
BsmI GAATG_Cn'

Cuts at:      0     445    1947 
   Size:        445    1502
 
Bsp1286I G_dGCh'C

Cuts at:      0     788     995    1947 
   Size:        788     207     952

  Fragments arranged by size: 

                 952     788     207
 
BsrDI GCAATG_nn'

Cuts at:      0    1622    1806    1947 
   Size:       1622     184     141

  Fragments arranged by size: 

                1622     184     141
 
BsrGI T'GTAC_A

Cuts at:      0    1517    1865    1947 
   Size:       1517     348      82

  Fragments arranged by size: 

                1517     348      82
 
BstBI TT'CG_AA

Cuts at:      0    1512    1947 
   Size:       1512     435
 
BstEII G'GTnAC_C

Cuts at:      0    1617    1947 
   Size:       1617     330
 
BstYI r'GATC_y

Cuts at:      0    1038    1348    1947 
   Size:       1038     310     599

  Fragments arranged by size: 

                1038     599     310
 
Bsu36I CC'TnA_GG

Cuts at:      0     745     860    1947 
   Size:        745     115    1087

  Fragments arranged by size: 

                1087     745     115
 
BtgI C'CryG_G

Cuts at:      0    1442    1947 
   Size:       1442     505
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     125    1947 
   Size:        125    1822
 
BtsI GCAGTG_nn'

Cuts at:      0    1100    1389    1947 
   Size:       1100     289     558

  Fragments arranged by size: 

                1100     558     289
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     969    1004    1120    1155    1947 
   Size:        969      35     116      35     792

  Fragments arranged by size: 

                 969     792     116      35      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     415     687    1947 
   Size:        415     272    1260

  Fragments arranged by size: 

                1260     415     272
 
EaeI y'GGCC_r

Cuts at:      0    1141    1947 
   Size:       1141     806
 
EarI CTCTTCn'nnn_

Cuts at:      0     431     587    1678    1947 
   Size:        431     156    1091     269

  Fragments arranged by size: 

                1091     431     269     156
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      36     178     212    1133    1426    1947 
   Size:         36     142      34     921     293     521

  Fragments arranged by size: 

                 921     521     293     142      36      34
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1130    1947 
   Size:       1130     817
 
EcoRI G'AATT_C

Cuts at:      0     702    1947 
   Size:        702    1245
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     397     429    1947 
   Size:        397      32    1518

  Fragments arranged by size: 

                1518     397      32
 
HincII GTy'rAC

Cuts at:      0     981    1947 
   Size:        981     966
 
HindIII A'AGCT_T

Cuts at:      0     751    1947 
   Size:        751    1196
 
MfeI C'AATT_G

Cuts at:      0    1364    1947 
   Size:       1364     583
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     492    1947 
   Size:        181     311    1455

  Fragments arranged by size: 

                1455     311     181
 
MscI TGG'CCA

Cuts at:      0    1143    1947 
   Size:       1143     804
 
MslI CAynn'nnrTG

Cuts at:      0      99     404    1661    1947 
   Size:         99     305    1257     286

  Fragments arranged by size: 

                1257     305     286      99
 
MspA1I CmG'CkG

Cuts at:      0    1316    1947 
   Size:       1316     631
 
NcoI C'CATG_G

Cuts at:      0    1442    1947 
   Size:       1442     505
 
NsiI A_TGCA'T

Cuts at:      0    1435    1947 
   Size:       1435     512
 
PasI CC'CwG_GG

Cuts at:      0     509    1947 
   Size:        509    1438
 
PfoI T'CCnGG_A

Cuts at:      0    1757    1947 
   Size:       1757     190
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1421    1453    1947 
   Size:       1421      32     494

  Fragments arranged by size: 

                1421     494      32
 
PstI C_TGCA'G

Cuts at:      0     426    1947 
   Size:        426    1521
 
PvuII CAG'CTG

Cuts at:      0    1316    1947 
   Size:       1316     631
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1678    1947 
   Size:       1678     269
 
ScaI AGT'ACT

Cuts at:      0    1390    1947 
   Size:       1390     557
 
SfcI C'TryA_G

Cuts at:      0     422     527    1905    1947 
   Size:        422     105    1378      42

  Fragments arranged by size: 

                1378     422     105      42
 
SmlI C'TyrA_G

Cuts at:      0    1218    1677    1947 
   Size:       1218     459     270

  Fragments arranged by size: 

                1218     459     270
 
SpeI A'CTAG_T

Cuts at:      0     595    1947 
   Size:        595    1352
 
StyI C'CwwG_G

Cuts at:      0    1131    1442    1947 
   Size:       1131     311     505

  Fragments arranged by size: 

                1131     505     311
 
TatI w'GTAC_w

Cuts at:      0    1388    1517    1865    1947 
   Size:       1388     129     348      82

  Fragments arranged by size: 

                1388     348     129      82
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1107    1139    1197    1229    1827    1859    1947 
   Size:       1107      32      58      32     598      32      88

  Fragments arranged by size: 

                1107     598      88      58      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0     615    1947 
   Size:        615    1332
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1581    1947 
   Size:       1581     366
 
XmnI GAAnn'nnTTC

Cuts at:      0    1016    1947 
   Size:       1016     931
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BanI    BanII     BcgI    BciVI     BclI
    BfrBI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BseRI    BseYI     BsgI     BsmI Bsp1286I    BsrDI    BsrGI
    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI
     DrdI     EaeI     EarI  Eco57MI    EcoNI    EcoRI    Hin4I   HincII
  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NsiI
     PasI     PfoI     PsrI     PstI    PvuII     SapI     ScaI     SfcI
     SmlI     SpeI     StyI     TatI     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BglI    BglII     BlpI    BmgBI     BmtI     BplI    BsaHI
    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstXI  BstZ17I
     ClaI     DraI   DraIII     EagI     EciI  EcoICRI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SphI     SrfI     SspI     StuI     SwaI    TaqII
  Tth111I     XhoI     XmaI     ZraI
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