Restriction map for pF1KA1464
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# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbsI        BbvCI       BclI        BfrI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KasI        KpnI        MamI        
MfeI        MluI        MroI        MunI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tsp45I      Tth111I     XbaI        XhoI        XmnI        


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# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcsI         1593  GAATTT               [GA]^AATT[CT]
Aor51HI       944  AGCGCT               AGC^GCT
ApoI         1593  GAATTT               [GA]^AATT[CT]
BcgI         1169  GCAATGGCGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         126  GTATCC               GTATCC(6/5)
BfaI         1399  CTAG                 C^TAG
BlpI          102  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      102  GCTGAGC              GC^T[ACGT]AGC
BsgI         1217  CTGCAC               GTGCAG(16/14)
BsmBI         497  GAGACG               CGTCTC(1/5)
BsmI          441  GCATTC               GAATGC(1/-1)
BsrDI        1169  GCAATG               GCAATG(2/0)
BssSI         793  CTCGTG               CACGAG(-5/-1)
BstAPI        759  GCAGGCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        421  GGTTACC              G^GT[ACGT]ACC
BstPI         421  GGTTACC              G^GT[ACGT]ACC
BstYI         890  AGATCC               [GA]^GATC[CT]
CelII         102  GCTGAGC              GC^T[ACGT]AGC
DraII        1445  AGGACCT              [GA]G^G[ACGT]CC[CT]
Eco47III      944  AGCGCT               AGC^GCT
EcoNI        1773  CCTGGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1445  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       421  GGTTACC              G^GT[ACGT]ACC
KspI          131  CCGCGG               CCGC^GG
MaeI         1399  CTAG                 C^TAG
MaeII         500  ACGT                 A^CGT
MflI          890  AGATCC               [GA]^GATC[CT]
MseI         1424  TTAA                 T^TAA
NcoI          426  CCATGG               C^CATGG
PflMI         894  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1445  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII        1161  CAGCTG               CAG^CTG
SacI         1053  GAGCTC               GAGCT^C
SacII         131  CCGCGG               CCGC^GG
SmaI           21  CCCGGG               CCC^GGG
TfiI          852  GATTC                G^A[AT]TC
Tru9I        1424  TTAA                 T^TAA
Van91I        894  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          426  CCATGGTGATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         890  AGATCC               [GA]^GATC[CT]
XmaCI          21  CCCGGG               C^CCGGG
XmaI           21  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AfaI         1331  GTAC                 GT^AC
             1584  GTAC                
AlwI          891  GATCC                GGATC(4/5)
             1529  GGATC               
BanI          532  GGCACC               G^G[CT][GA]CC
             1429  GGTGCC              
BanII         171  GAGCCC               G[GA]GC[CT]^C
             1053  GAGCTC              
BglI          914  GCCGTGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1661  GCCCAGTTGGC         
BsrFI         107  GCCGGC               [GA]^CCGG[CT]
             1001  GCCGGC              
BstXI         468  CCAGCCCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              558  CCACAGCCTTGG        
Cfr10I        107  GCCGGC               [GA]^CCGG[CT]
             1001  GCCGGC              
DrdI         1033  GACAGTGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1455  GACGGATGAGTC        
NaeI          107  GCCGGC               GCC^GGC
             1001  GCCGGC              
NgoMIV        107  GCCGGC               G^CCGGC
             1001  GCCGGC              
RsaI         1331  GTAC                 GT^AC
             1584  GTAC                
SmlI          371  CTCAAG               C^T[CT][GA]AG
              994  CTCAAG              
StuI          612  AGGCCT               AGG^CCT
             1815  AGGCCT              
TaqI          834  TCGA                 T^CGA
              932  TCGA                
Tsp509I      1279  AATT                 ^AATT
             1594  AATT                
TthHB8I       834  TCGA                 T^CGA
              932  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwNI       
AspHI       AvaI        AvaII       BalI        BbvI        
BcnI        BmyI        BpmI        BsaHI       BsaI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DsaI        EaeI        EarI        EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TseI        TspRI       
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