Restriction map for pF1KA1464
 (Linear) (Six-base) MAPSORT of: KIAA1464.seq  Check: 5864  from: 1  to: 1860



KIAA1464.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     544    1860 
   Size:        544    1316
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1611    1860 
   Size:       1611     249
 
AfeI AGC'GCT

Cuts at:      0     946    1860 
   Size:        946     914
 
AlwNI CAG_nnn'CTG

Cuts at:      0     102     123     900    1743    1860 
   Size:        102      21     777     843     117

  Fragments arranged by size: 

                 843     777     117     102      21
 
ApoI r'AATT_y

Cuts at:      0    1593    1860 
   Size:       1593     267
 
AvaI C'yCGr_G

Cuts at:      0      21     456    1808    1860 
   Size:         21     435    1352      52

  Fragments arranged by size: 

                1352     435      52      21
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1314    1347    1860 
   Size:       1314      33     513

  Fragments arranged by size: 

                1314     513      33
 
BanI G'GyrC_C

Cuts at:      0     532    1429    1860 
   Size:        532     897     431

  Fragments arranged by size: 

                 897     532     431
 
BanII G_rGCy'C

Cuts at:      0     175    1057    1860 
   Size:        175     882     803

  Fragments arranged by size: 

                 882     803     175
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1158    1192    1860 
   Size:       1158      34     668

  Fragments arranged by size: 

                1158     668      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     137    1860 
   Size:        137    1723
 
BglI GCCn_nnn'nGGC

Cuts at:      0     920    1667    1860 
   Size:        920     747     193

  Fragments arranged by size: 

                 920     747     193
 
BlpI GC'TnA_GC

Cuts at:      0     103    1860 
   Size:        103    1757
 
Bme1580I G_kGCm'C

Cuts at:      0     718     744    1860 
   Size:        718      26    1116

  Fragments arranged by size: 

                1116     718      26
 
BmgBI CAC'GTC

Cuts at:      0     501    1860 
   Size:        501    1359
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1115    1657    1860 
   Size:       1115     542     203

  Fragments arranged by size: 

                1115     542     203
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1311    1860 
   Size:       1311     549
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     928     985    1674    1860 
   Size:        189     739      57     689     186

  Fragments arranged by size: 

                 739     689     189     186      57
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     979    1860 
   Size:        356     623     881

  Fragments arranged by size: 

                 881     623     356
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     205     928    1339    1860 
   Size:        205     723     411     521

  Fragments arranged by size: 

                 723     521     411     205
 
BsaHI Gr'CG_yC

Cuts at:      0     377     641     868    1175    1860 
   Size:        377     264     227     307     685

  Fragments arranged by size: 

                 685     377     307     264     227
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1075    1105    1860 
   Size:       1075      30     755

  Fragments arranged by size: 

                1075     755      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      42     444     973    1218    1233    1236    1239    1242 
   Size:         42     402     529     245      15       3       3       3

Cuts at:   1242    1860 
   Size:        618

  Fragments arranged by size: 

                 618     529     402     245      42      15       3       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0      28      67     941    1096    1159    1222    1316    1860 
   Size:         28      39     874     155      63      63      94     544

  Fragments arranged by size: 

                 874     544     155      94      63      63      39      28
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1202    1860 
   Size:       1202     658
 
BsiHKAI G_wGCw'C

Cuts at:      0     971    1057    1382    1860 
   Size:        971      86     325     478

  Fragments arranged by size: 

                 971     478     325      86
 
BsmI GAATG_Cn'

Cuts at:      0     441    1860 
   Size:        441    1419
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     491    1860 
   Size:        491    1369
 
Bsp1286I G_dGCh'C

Cuts at:      0     175     718     744     971    1057    1382    1860 
   Size:        175     543      26     227      86     325     478

  Fragments arranged by size: 

                 543     478     325     227     175      86      26
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     297     544    1457    1751    1860 
   Size:        297     247     913     294     109

  Fragments arranged by size: 

                 913     297     294     247     109
 
BsrDI GCAATG_nn'

Cuts at:      0    1176    1860 
   Size:       1176     684
 
BsrFI r'CCGG_y

Cuts at:      0     107    1001    1860 
   Size:        107     894     859

  Fragments arranged by size: 

                 894     859     107
 
BssSI C'ACGA_G

Cuts at:      0     793    1860 
   Size:        793    1067
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     765    1860 
   Size:        765    1095
 
BstEII G'GTnAC_C

Cuts at:      0     421    1860 
   Size:        421    1439
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     475     565    1860 
   Size:        475      90    1295

  Fragments arranged by size: 

                1295     475      90
 
BstYI r'GATC_y

Cuts at:      0     890    1860 
   Size:        890     970
 
BtgI C'CryG_G

Cuts at:      0     131     426     821    1860 
   Size:        131     295     395    1039

  Fragments arranged by size: 

                1039     395     295     131
 
BtsI GCAGTG_nn'

Cuts at:      0     723     770    1860 
   Size:        723      47    1090

  Fragments arranged by size: 

                1090     723      47
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     810     845    1401    1436    1860 
   Size:        810      35     556      35     424

  Fragments arranged by size: 

                 810     556     424      35      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1039    1461    1860 
   Size:       1039     422     399

  Fragments arranged by size: 

                1039     422     399
 
EaeI y'GGCC_r

Cuts at:      0     465     555     590    1542    1668    1860 
   Size:        465      90      35     952     126     192

  Fragments arranged by size: 

                 952     465     192     126      90      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     333     991    1266    1860 
   Size:        333     658     275     594

  Fragments arranged by size: 

                 658     594     333     275
 
EcoICRI GAG'CTC

Cuts at:      0    1055    1860 
   Size:       1055     805
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     928     985    1611    1674    1860 
   Size:        189     739      57     626      63     186

  Fragments arranged by size: 

                 739     626     189     186      63      57
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1777    1860 
   Size:       1777      83
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1446    1860 
   Size:       1446     414
 
HaeII r_GCGC'y

Cuts at:      0      68     114     123     948    1724    1860 
   Size:         68      46       9     825     776     136

  Fragments arranged by size: 

                 825     776     136      68      46       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     247     279     488     520     875     907    1860 
   Size:        247      32     209      32     355      32     953

  Fragments arranged by size: 

                 953     355     247     209      32      32      32
 
HincII GTy'rAC

Cuts at:      0     138     381     519    1860 
   Size:        138     243     138    1341

  Fragments arranged by size: 

                1341     243     138     138
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1316    1860 
   Size:       1316     544
 
MscI TGG'CCA

Cuts at:      0     467     557    1670    1860 
   Size:        467      90    1113     190

  Fragments arranged by size: 

                1113     467     190      90
 
MslI CAynn'nnrTG

Cuts at:      0     254     416     431    1609    1788    1860 
   Size:        254     162      15    1178     179      72

  Fragments arranged by size: 

                1178     254     179     162      72      15
 
MspA1I CmG'CkG

Cuts at:      0     133     156    1163    1860 
   Size:        133      23    1007     697

  Fragments arranged by size: 

                1007     697     133      23
 
NaeI GCC'GGC

Cuts at:      0     109    1003    1860 
   Size:        109     894     857

  Fragments arranged by size: 

                 894     857     109
 
NcoI C'CATG_G

Cuts at:      0     426    1860 
   Size:        426    1434
 
NgoMIV G'CCGG_C

Cuts at:      0     107    1001    1860 
   Size:        107     894     859

  Fragments arranged by size: 

                 894     859     107
 
NspI r_CATG'y

Cuts at:      0     690    1137    1410    1860 
   Size:        690     447     273     450

  Fragments arranged by size: 

                 690     450     447     273
 
PasI CC'CwG_GG

Cuts at:      0      88     207     955    1773    1860 
   Size:         88     119     748     818      87

  Fragments arranged by size: 

                 818     748     119      88      87
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     900    1860 
   Size:        900     960
 
PfoI T'CCnGG_A

Cuts at:      0     166    1860 
   Size:        166    1694
 
PpuMI rG'GwC_Cy

Cuts at:      0    1446    1860 
   Size:       1446     414
 
PstI C_TGCA'G

Cuts at:      0     620     773    1454    1619    1860 
   Size:        620     153     681     165     241

  Fragments arranged by size: 

                 681     620     241     165     153
 
PvuII CAG'CTG

Cuts at:      0    1163    1860 
   Size:       1163     697
 
SacI G_AGCT'C

Cuts at:      0    1057    1860 
   Size:       1057     803
 
SacII CC_GC'GG

Cuts at:      0     134    1860 
   Size:        134    1726
 
SfcI C'TryA_G

Cuts at:      0     616     769    1164    1450    1615    1860 
   Size:        616     153     395     286     165     245

  Fragments arranged by size: 

                 616     395     286     245     165     153
 
SmaI CCC'GGG

Cuts at:      0      23    1860 
   Size:         23    1837
 
SmlI C'TyrA_G

Cuts at:      0     371     994    1860 
   Size:        371     623     866

  Fragments arranged by size: 

                 866     623     371
 
StuI AGG'CCT

Cuts at:      0     614    1817    1860 
   Size:        614    1203      43

  Fragments arranged by size: 

                1203     614      43
 
StyI C'CwwG_G

Cuts at:      0     176     426     564     704    1860 
   Size:        176     250     138     140    1156

  Fragments arranged by size: 

                1156     250     176     140     138
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     292     955    1236    1860 
   Size:        292     663     281     624

  Fragments arranged by size: 

                 663     624     292     281
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1020    1052    1687    1719    1860 
   Size:       1020      32     635      32     141

  Fragments arranged by size: 

                1020     635     141      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     433    1860 
   Size:        433    1427
 
XmaI C'CCGG_G

Cuts at:      0      21    1860 
   Size:         21    1839
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AlwNI     ApoI     AvaI     BaeI     BanI
    BanII     BcgI    BciVI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrDI    BsrFI
    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI     BtsI    CspCI
     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NspI     PasI    PflMI     PfoI    PpuMI     PstI    PvuII
     SacI    SacII     SfcI     SmaI     SmlI     StuI     StyI    TaqII
     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII    BamHI     BbeI     BbsI    BbvCI     BclI    BfrBI    BglII
     BmtI   Bpu10I    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI    BspEI
    BspHI    BsrBI    BsrGI   BssHII    BstBI  BstZ17I   Bsu36I    BtgZI
     ClaI     DraI   DraIII     EagI     EciI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TatI  Tth111I     XbaI     XhoI     XmnI
     ZraI
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