Restriction map for pF1KA1452
 (Linear) (Six-base) MAPSORT of: KIAA1452.seq  Check: 9933  from: 1  to: 2124



KIAA1452.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     629    2124 
   Size:        629    1495
 
Acc65I G'GTAC_C

Cuts at:      0    2099    2124 
   Size:       2099      25
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1705    1779    2124 
   Size:       1705      74     345

  Fragments arranged by size: 

                1705     345      74
 
AflIII A'CryG_T

Cuts at:      0    1415    2124 
   Size:       1415     709
 
AleI CACnn'nnGTG

Cuts at:      0    1669    2124 
   Size:       1669     455
 
AlwNI CAG_nnn'CTG

Cuts at:      0     672    1351    1857    2124 
   Size:        672     679     506     267

  Fragments arranged by size: 

                 679     672     506     267
 
ApaI G_GGCC'C

Cuts at:      0     907    2124 
   Size:        907    1217
 
ApoI r'AATT_y

Cuts at:      0    1964    2124 
   Size:       1964     160
 
AvaI C'yCGr_G

Cuts at:      0    1606    1676    2124 
   Size:       1606      70     448

  Fragments arranged by size: 

                1606     448      70
 
BamHI G'GATC_C

Cuts at:      0    1379    2124 
   Size:       1379     745
 
BanI G'GyrC_C

Cuts at:      0    1461    1640    1860    2099    2124 
   Size:       1461     179     220     239      25

  Fragments arranged by size: 

                1461     239     220     179      25
 
BanII G_rGCy'C

Cuts at:      0     371     611     713     907    1502    1553    2124 
   Size:        371     240     102     194     595      51     571

  Fragments arranged by size: 

                 595     571     371     240     194     102      51
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     506    2124 
   Size:        506    1618
 
BbvCI CC'TCA_GC

Cuts at:      0    1037    2124 
   Size:       1037    1087
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1285    2124 
   Size:       1285     839
 
BclI T'GATC_A

Cuts at:      0    1938    2124 
   Size:       1938     186
 
BglI GCCn_nnn'nGGC

Cuts at:      0     469     902    1356    1608    2124 
   Size:        469     433     454     252     516

  Fragments arranged by size: 

                 516     469     454     433     252
 
BglII A'GATC_T

Cuts at:      0    2034    2124 
   Size:       2034      90
 
BlpI GC'TnA_GC

Cuts at:      0    1732    2124 
   Size:       1732     392
 
Bme1580I G_kGCm'C

Cuts at:      0     682     907    1624    1643    2124 
   Size:        682     225     717      19     481

  Fragments arranged by size: 

                 717     682     481     225      19
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      16     719     981    2124 
   Size:         16     703     262    1143

  Fragments arranged by size: 

                1143     703     262      16
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2053    2124 
   Size:       2053      71
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1177    1444    1946    2124 
   Size:       1177     267     502     178

  Fragments arranged by size: 

                1177     502     267     178
 
Bpu10I CC'TnA_GC

Cuts at:      0    1037    2124 
   Size:       1037    1087
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     672    1726    2003    2124 
   Size:        193     479    1054     277     121

  Fragments arranged by size: 

                1054     479     277     193     121
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     256    2124 
   Size:        256    1868
 
BsaAI yAC'GTr

Cuts at:      0     272    2124 
   Size:        272    1852
 
BsaBI GATnn'nnATC

Cuts at:      0     181     847    1943    2124 
   Size:        181     666    1096     181

  Fragments arranged by size: 

                1096     666     181     181
 
BsaHI Gr'CG_yC

Cuts at:      0     437    2124 
   Size:        437    1687
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     619     778     781    1510    1525    1534    1543    1558 
   Size:        619     159       3     729      15       9       9      15

Cuts at:   1558    1561    2124 
   Size:          3     563

  Fragments arranged by size: 

                 729     619     563     159      15      15       9       9
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0     409     754     790     812    1091    1190    1403    1567 
   Size:        409     345      36      22     279      99     213     164

Cuts at:   1567    2124 
   Size:        557

  Fragments arranged by size: 

                 557     409     345     279     213     164      99      36
                  22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     589    1744    2124 
   Size:        332     257    1155     380

  Fragments arranged by size: 

                1155     380     332     257
 
BsiEI CG_ry'CG

Cuts at:      0     538    2124 
   Size:        538    1586
 
BsiHKAI G_wGCw'C

Cuts at:      0     371     713    1046    1163    1727    2124 
   Size:        371     342     333     117     564     397

  Fragments arranged by size: 

                 564     397     371     342     333     117
 
Bsp1286I G_dGCh'C

Cuts at:      0     371     611     682     713     907    1046    1163    1502 
   Size:        371     240      71      31     194     139     117     339

Cuts at:   1502    1553    1624    1643    1727    2124 
   Size:         51      71      19      84     397

  Fragments arranged by size: 

                 397     371     339     240     194     139     117      84
                  71      71      51      31      19
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     629     680    2124 
   Size:        629      51    1444

  Fragments arranged by size: 

                1444     629      51
 
BsrBI CCG'CTC

Cuts at:      0    1428    2124 
   Size:       1428     696
 
BsrFI r'CCGG_y

Cuts at:      0    1840    2124 
   Size:       1840     284
 
BsrGI T'GTAC_A

Cuts at:      0      27    2124 
   Size:         27    2097
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1260    2124 
   Size:       1260     864
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      60    2124 
   Size:         60    2064
 
BstYI r'GATC_y

Cuts at:      0     848    1379    2034    2124 
   Size:        848     531     655      90

  Fragments arranged by size: 

                 848     655     531      90
 
Bsu36I CC'TnA_GG

Cuts at:      0     637    2124 
   Size:        637    1487
 
BtgI C'CryG_G

Cuts at:      0     920    1169    1553    2124 
   Size:        920     249     384     571

  Fragments arranged by size: 

                 920     571     384     249
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     967    2124 
   Size:        967    1157
 
ClaI AT'CG_AT

Cuts at:      0      38     914    2124 
   Size:         38     876    1210

  Fragments arranged by size: 

                1210     876      38
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     677     712    2124 
   Size:        677      35    1412

  Fragments arranged by size: 

                1412     677      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     727    2124 
   Size:        727    1397
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2118    2124 
   Size:       2118       6
 
EaeI y'GGCC_r

Cuts at:      0       2      50     179     461     535     833    1166    1589 
   Size:          2      48     129     282      74     298     333     423

Cuts at:   1589    1766    1782    1835    2124 
   Size:        177      16      53     289

  Fragments arranged by size: 

                 423     333     298     289     282     177     129      74
                  53      48      16       2
 
EagI C'GGCC_G

Cuts at:      0     535    2124 
   Size:        535    1589
 
EarI CTCTTCn'nnn_

Cuts at:      0       5     315     486     976    1756    1798    2124 
   Size:          5     310     171     490     780      42     326

  Fragments arranged by size: 

                 780     490     326     310     171      42       5
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      79     511    1458    1555    2124 
   Size:         79     432     947      97     569

  Fragments arranged by size: 

                 947     569     432      97      79
 
EcoICRI GAG'CTC

Cuts at:      0     369     711    2124 
   Size:        369     342    1413

  Fragments arranged by size: 

                1413     369     342
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1177    1444    1705    1779    1946    2124 
   Size:       1177     267     261      74     167     178

  Fragments arranged by size: 

                1177     267     261     178     167      74
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     427    2124 
   Size:        427    1697
 
EcoO109I rG'GnC_Cy

Cuts at:      0     248     904    1348    2124 
   Size:        248     656     444     776

  Fragments arranged by size: 

                 776     656     444     248
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     318    1767    2124 
   Size:        318    1449     357

  Fragments arranged by size: 

                1449     357     318
 
FspI TGC'GCA

Cuts at:      0     820    2124 
   Size:        820    1304
 
HaeII r_GCGC'y

Cuts at:      0     564    1749    2124 
   Size:        564    1185     375

  Fragments arranged by size: 

                1185     564     375
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     630     634     662     666    1327    1359    1992    2024 
   Size:        630       4      28       4     661      32     633      32

Cuts at:   2024    2124 
   Size:        100

  Fragments arranged by size: 

                 661     633     630     100      32      32      28       4
                   4
 
HincII GTy'rAC

Cuts at:      0    2015    2124 
   Size:       2015     109
 
KpnI G_GTAC'C

Cuts at:      0    2103    2124 
   Size:       2103      21
 
MluI A'CGCG_T

Cuts at:      0    1415    2124 
   Size:       1415     709
 
MscI TGG'CCA

Cuts at:      0       4      52     181    1591    1768    2124 
   Size:          4      48     129    1410     177     356

  Fragments arranged by size: 

                1410     356     177     129      48       4
 
MslI CAynn'nnrTG

Cuts at:      0     629    1168    1238    1669    2124 
   Size:        629     539      70     431     455

  Fragments arranged by size: 

                 629     539     455     431      70
 
MspA1I CmG'CkG

Cuts at:      0     346     402     816    1025    1091    1251    1377    1440 
   Size:        346      56     414     209      66     160     126      63

Cuts at:   1440    1455    1571    1799    2124 
   Size:         15     116     228     325

  Fragments arranged by size: 

                 414     346     325     228     209     160     126     116
                  66      63      56      15
 
NcoI C'CATG_G

Cuts at:      0    1553    2124 
   Size:       1553     571
 
NspI r_CATG'y

Cuts at:      0    1263    1818    2097    2124 
   Size:       1263     555     279      27

  Fragments arranged by size: 

                1263     555     279      27
 
PasI CC'CwG_GG

Cuts at:      0     611    1650    2124 
   Size:        611    1039     474

  Fragments arranged by size: 

                1039     611     474
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     948    2124 
   Size:        948    1176
 
PfoI T'CCnGG_A

Cuts at:      0    1154    2124 
   Size:       1154     970
 
PspOMI G'GGCC_C

Cuts at:      0     903    2124 
   Size:        903    1221
 
PstI C_TGCA'G

Cuts at:      0     401     590    1051    1439    2124 
   Size:        401     189     461     388     685

  Fragments arranged by size: 

                 685     461     401     388     189
 
PvuII CAG'CTG

Cuts at:      0     402     816    1455    2124 
   Size:        402     414     639     669

  Fragments arranged by size: 

                 669     639     414     402
 
RsrII CG'GwC_CG

Cuts at:      0    1809    2124 
   Size:       1809     315
 
SacI G_AGCT'C

Cuts at:      0     371     713    2124 
   Size:        371     342    1411

  Fragments arranged by size: 

                1411     371     342
 
SapI GCTCTTCn'nnn_

Cuts at:      0     315    2124 
   Size:        315    1809
 
ScaI AGT'ACT

Cuts at:      0    2063    2124 
   Size:       2063      61
 
SfcI C'TryA_G

Cuts at:      0     354     397     586    1047    1435    1819    2124 
   Size:        354      43     189     461     388     384     305

  Fragments arranged by size: 

                 461     388     384     354     305     189      43
 
SmlI C'TyrA_G

Cuts at:      0     172     687    1741    2018    2124 
   Size:        172     515    1054     277     106

  Fragments arranged by size: 

                1054     515     277     172     106
 
SphI G_CATG'C

Cuts at:      0    1818    2124 
   Size:       1818     306
 
StuI AGG'CCT

Cuts at:      0    1690    2124 
   Size:       1690     434
 
StyI C'CwwG_G

Cuts at:      0     963    1001    1397    1553    2124 
   Size:        963      38     396     156     571

  Fragments arranged by size: 

                 963     571     396     156      38
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     738     768     963    1083    2124 
   Size:        738      30     195     120    1041

  Fragments arranged by size: 

                1041     738     195     120      30
 
TatI w'GTAC_w

Cuts at:      0      27      44    2061    2115    2124 
   Size:         27      17    2017      54       9

  Fragments arranged by size: 

                2017      54      27      17       9
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     918    1668    2124 
   Size:        918     750     456

  Fragments arranged by size: 

                 918     750     456
 
XmnI GAAnn'nnTTC

Cuts at:      0    1150    1690    2124 
   Size:       1150     540     434

  Fragments arranged by size: 

                1150     540     434
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AleI    AlwNI     ApaI     ApoI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI    BsrGI   BstAPI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI     FspI    HaeII    Hin4I   HincII     KpnI     MluI
     MscI     MslI   MspA1I     NcoI     NspI     PasI    PflMI     PfoI
   PspOMI     PstI    PvuII    RsrII     SacI     SapI     ScaI     SfcI
     SmlI     SphI     StuI     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
     BcgI    BfrBI    BmgBI     BmtI    BsaWI    BsaXI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI   BssHII    BssSI    BstBI   BstEII
  BstZ17I     BtsI     DraI    EcoRI    EcoRV     FseI    FspAI  HindIII
     HpaI     KasI     MfeI     MmeI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    SacII
     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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